scholarly journals Pituitary follicular cells secrete an inhibitor of aortic endothelial cell growth: identification as leukemia inhibitory factor.

1992 ◽  
Vol 89 (2) ◽  
pp. 698-702 ◽  
Author(s):  
N. Ferrara ◽  
J. Winer ◽  
W. J. Henzel
Keyword(s):  
Endothelial Cell ◽  
Cell Growth ◽  
Leukemia Inhibitory Factor ◽  
Inhibitory Factor ◽  
Follicular Cells ◽  
Aortic Endothelial Cell ◽  
Endothelial Cell Growth ◽  
Aortic Endothelial

scholarly journals Bovine pericardial extracellular matrix niche modulates human aortic endothelial cell phenotype and function

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jeny Shklover ◽  
James McMasters ◽  
Alba Alfonso-Garcia ◽  
Manuela Lopera Higuita ◽  
Alyssa Panitch ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cell ◽  
Cell Growth ◽  
Basement Membrane ◽  
Inflammatory Marker ◽  
Cell Phenotype ◽  
Human Aortic Endothelial Cell ◽  
Aortic Endothelial Cell ◽  
And Function ◽  
Aortic Endothelial

Abstract Xenogeneic biomaterials contain biologically relevant extracellular matrix (ECM) composition and organization, making them potentially ideal surgical grafts and tissue engineering scaffolds. Defining the effect of ECM niche (e.g., basement membrane vs. non-basement membrane) on repopulating cell phenotype and function has important implications for use of xenogeneic biomaterials, particularly in vascular applications. We aim to understand how serous (i.e., basement membrane) versus fibrous (i.e., non-basement membrane) ECM niche of antigen-removed bovine pericardium (AR-BP) scaffolds influence human aortic endothelial cell (hAEC) adhesion, growth, phenotype, inflammatory response and laminin production. At low and moderate seeding densities hAEC proliferation was significantly increased on the serous side. Similarly, ECM niche modulated cellular morphology, with serous side seeding resulting in a more rounded aspect ratio and intact endothelial layer formation. At moderate seeding densities, hAEC production of human laminin was enhanced following serous seeding. Finally, inflammatory marker and pro-inflammatory cytokine expression decreased following long-term cell growth regardless of seeding side. This work demonstrates that at low and moderate seeding densities AR-BP sidedness significantly impacts endothelial cell growth, morphology, human laminin production, and inflammatory state. These findings suggest that ECM niche has a role in modulating response of repopulating recipient cells toward AR-BP scaffolds for vascular applications.

scholarly journals Effects of angiotensin II on endothelial cell growth: role of AT-1 and AT-2 receptors.

1998 ◽  
Vol 9 (6) ◽  
pp. 969-974
Author(s):  
M Montón ◽  
M A Castilla ◽  
M V Alvarez Arroyo ◽  
D Tan ◽  
F R González-Pacheco ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Angiotensin Ii ◽  
Cell Growth ◽  
Endothelial Cell Proliferation ◽  
Bovine Aortic Endothelial Cell ◽  
Promoting Effect ◽  
Aortic Endothelial Cells ◽  
Endothelial Cell Growth ◽  
Aortic Endothelial

Angiotensin II (AngII) is a main mediator in the regulation of vascular tone. Although its effects on vascular smooth muscle cells are well known, data on its role on endothelial biology are still insufficient. The present study examined the effect of endogenous and exogenous AngII on bovine aortic endothelial cells possessing both AT-1 and AT-2 receptors. A DNA synthesis-promoting effect of AT-2 blockade by PD123319 (10(-9) to 10(-7) M) was demonstrated. This effect was transduced through an AT-1-mediated pathway, as shown by using the AT-1 antagonist, losartan. In addition, an AT-1-mediated effect of AngII was demonstrated on bovine aortic endothelial cell proliferation, which occurred despite the absence of AngII-induced Ca2+ transients. In summary, the present study disclosed relevant characteristics of the effect of AngII on endothelial cell growth that have potential pathophysiologic projections, particularly for the use of selective AngII blocking agents.

Platelet-derived endothelial cell growth factor mediates angiogenesis and antiapoptosis in rat aortic endothelial cells

2009 ◽  
Vol 87 (6) ◽  
pp. 883-893 ◽  
Author(s):  
Thiagarajan Hemalatha ◽  
Mitali Tiwari ◽  
Chidambaram Balachandran ◽  
Bhakthavatsalam Murali Manohar ◽  
Rengarajulu Puvanakrishnan
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Hypoxic Stress ◽  
Tubule Formation ◽  
Aortic Endothelial Cells ◽  
Endothelial Cell Growth Factor ◽  
Endothelial Cell Growth ◽  
Aortic Endothelial

This study explores the angiogenic and antiapoptotic activities of platelet-derived endothelial cell growth factor (PDECGF) in rat aortic endothelial cells. The effects of PDECGF on rat aortic endothelial cell (RAEC) proliferation, migration, chemotaxis, and tubule formation were investigated in vitro at various concentrations viz., 1, 2, 4, 8, 16, and 32 ng·mL–1 on endothelial cells. Endothelial cells were induced with hypoxic stress and the antiapoptotic effects of PDECGF were analysed by cell survival assay, fluorescence microscopy, cell viability assay, and flow cytometry. The results demonstrated the angiogenic potential of PDECGF on endothelial cells in a dose-dependent manner. PDECGF at 16 and 32 ng·mL–1 increased cell proliferation (>80%), induced cell migration (>4 fold), stimulated chemotaxis (>2 fold), and increased tubule formation (>3 fold) compared with the control. Studies on hypoxic stress revealed the antiapoptotic nature of PDECGF on endothelial cells. PDECGF treatment enhanced cell survival by 14%, as well as cell viability by 13%, and decreased the percentage of apoptotic cells by 13% as demonstrated by fluorescence-activated cell sorter studies (FACS). In conclusion, this study demonstrated the angiogenic and antiapoptotic potentials of PDECGF on RAEC.

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