scholarly journals Two oncogenes, v-fos and v-ras, cooperate to convert normal keratinocytes to squamous cell carcinoma.

1990 ◽  
Vol 87 (2) ◽  
pp. 643-647 ◽  
Author(s):  
D. A. Greenhalgh ◽  
D. J. Welty ◽  
A. Player ◽  
S. H. Yuspa
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Keratinocytes

Marker profiling of normal keratinocytes identifies the stroma from squamous cell carcinoma of the oral cavity as a modulatory microenvironment in co-culture

2007 ◽  
Vol 83 (11-12) ◽  
pp. 837-848 ◽  
Author(s):  
Lukas Lacina ◽  
Barbora Dvořánkova ◽  
Karel Smetana ◽  
Martin Chovanec ◽  
Jan Plzák ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cavity ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Keratinocytes

scholarly journals Wnt/CTNNB1 Signal Transduction Pathway Inhibits the Expression of ZFP36 in Squamous Cell Carcinoma, by Inducing Transcriptional Repressors SNAI1, SLUG and TWIST

2020 ◽  
Vol 21 (16) ◽  
pp. 5692
Author(s):  
Emma D. Zanfi ◽  
Sebastian Fantini ◽  
Roberta Lotti ◽  
Matteo Bertesi ◽  
Alessandra Marconi ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Signal Transduction Pathway ◽  
Transcriptional Repressors ◽  
Gene Encoding ◽  
Epithelial Cancers ◽  
Normal Keratinocytes ◽  
Mrna Binding Protein ◽  
Mrna Binding

The Wnt/CTNNB1 pathway is often deregulated in epithelial tumors. The ZFP36 gene, encoding the mRNA binding protein Tristetraprolin (TTP), is downregulated in several cancers, where it has been described to behave as a tumor suppressor. By this report, we show that Wnt/CTNNB1 pathway is constitutively activated, and ZFP36 expression is downregulated in Squamous Cell Carcinoma (SCC) cell lines compared to normal keratinocytes. Moreover, we suggest that the decrease of ZFP36 expression might depend on the activity of transcriptional repressors SNAI1, SLUG and TWIST, whose expression is induced by Wnt/CTNNB1, highlighting a potential regulatory mechanism underlying ZFP36 downregulation in epithelial cancers.

scholarly journals The Comparison of In Vitro Photosensitizing Efficacy of Curcumin-Loaded Liposomes Following Photodynamic Therapy on Melanoma MUG-Mel2, Squamous Cell Carcinoma SCC-25, and Normal Keratinocyte HaCaT Cells

2021 ◽  
Vol 14 (4) ◽  
pp. 374
Author(s):  
Marta Woźniak ◽  
Martyna Nowak ◽  
Anastasiia Lazebna ◽  
Kamil Więcek ◽  
Izabella Jabłońska ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Skin ◽  
Human Keratinocytes ◽  
Malignant Cell ◽  
Liposomal Formulation ◽  
Normal Keratinocytes

The research focused on the investigation of curcumin encapsulated in hydrogenated soy phosphatidylcholine liposomes and its increased photoactive properties in photodynamic therapy (PDT). The goal of this study was two-fold: to emphasize the role of a natural photoactive plant-based derivative in the liposomal formulation as an easily bioavailable, alternative photosensitizer (PS) for the use in PDT of skin malignancies. Furthermore, the goal includes to prove the decreased cytotoxicity of phototoxic agents loaded in liposomes toward normal skin cells. Research was conducted on melanoma (MugMel2), squamous cell carcinoma (SCC-25), and normal human keratinocytes (HaCaT) cell lines. The assessment of viability with MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) evaluated cell death after exposure to blue light irradiation after 4 h of pre-incubation with free and encapsulated curcumin. Additionally, the wound healing assay, flow cytometry, and immunocytochemistry to detect apoptosis were performed. The malignant cells revealed increased phototoxicity after the therapy in comparison to normal cells. Moreover, liposome curcumin-based photodynamic therapy showed an increased ratio of apoptotic and necrotic cells. The study also demonstrated that nanocurcumin significantly decreased malignant cell motility following PDT treatment. Acquired results suggest that liposomal formulation of a poor soluble natural compound may improve photosensitizing properties of curcumin-mediated PDT treatment in skin cancers and reduce toxicity in normal keratinocytes.

scholarly journals Phenethyl Isothiocyanate-Containing Carbomer Gel for Use against Squamous Cell Carcinoma

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 106
Author(s):  
Ositomiwa O. Osipitan ◽  
Yi Shi ◽  
Anthony J. Di Pasqua
Keyword(s):  
Squamous Cell Carcinoma ◽  
Skin Cancer ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cancer Drug ◽  
Phenethyl Isothiocyanate ◽  
Topical Gel ◽  
Skin Cells ◽  
Normal Keratinocytes ◽  
Carbomer Gel

It is currently estimated that one in every five Americans will develop skin cancer during their lifetime. Squamous cell carcinoma (SCC) is a common type of skin cancer that can develop due to the skin’s exposure to the sun. Herein, we prepared a topical gel containing 0.5% v/w phenethyl isothiocyanate (PEITC) for the treatment of SCC. PEITC is a naturally occurring isothiocyanate that has been shown to have efficacy against various types of cancer in preclinical studies. We first incorporated PEITC into a carbomer gel. A uniform formulation was prepared, and its viscosity was appropriate for topical application. We then demonstrated the release of PEITC from the gel into and through a Strat-M skin-like membrane. Finally, the effects of the PEITC-containing gel were tested against SCC and normal keratinocytes skin cells in culture, and these results were compared to those obtained for free 5-fluoruracil (5-FU), a commonly used skin-cancer drug. Our results show that a homogeneous PEITC-containing topical gel can be prepared and used to kill SCC cells. Thus, our formulation may be useful for treating SCC in the clinic.

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