scholarly journals Base substitutions, frameshifts, and small deletions constitute ionizing radiation-induced point mutations in mammalian cells.

1988 ◽  
Vol 85 (1) ◽  
pp. 185-188 ◽  
Author(s):  
A. J. Grosovsky ◽  
J. G. de Boer ◽  
P. J. de Jong ◽  
E. A. Drobetsky ◽  
B. W. Glickman
Keyword(s):  
Ionizing Radiation ◽  
Mammalian Cells ◽  
Point Mutations ◽  
Base Substitutions ◽  
Radiation Induced

scholarly journals Molecular Markers of Ionizing Radiation-Induced Gene Mutations in Mammalian Cells

1996 ◽  
Vol 104 ◽  
pp. 675 ◽  
Author(s):  
Abraham W. Hsie ◽  
Ronald C. Porter ◽  
Zhidong Xu ◽  
Yonjia Yu ◽  
Juan Sun ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ionizing Radiation ◽  
Mammalian Cells ◽  
Gene Mutations ◽  
Radiation Induced

scholarly journals Hydrogen peroxide mediates the radiation-induced mutator phenotype in mammalian cells

2008 ◽  
Vol 413 (1) ◽  
pp. 185-191 ◽  
Author(s):  
Disha Dayal ◽  
Sean M. Martin ◽  
Charles L. Limoli ◽  
Douglas R. Spitz
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Ionizing Radiation ◽  
Mammalian Cells ◽  
Mutation Frequency ◽  
Fold Increase ◽  
Mutator Phenotype ◽  
Chronic Oxidative Stress ◽  
Dose Dependent Fashion ◽  
Radiation Induced

Chronic oxidative stress has been associated with genomic instability following exposure to ionizing radiation. However, results showing direct causal linkages between specific ROS (reactive oxygen species) and the ionizing radiation-induced mutator phenotype are lacking. The present study demonstrates that ionizing radiation-induced genomically unstable cells (characterized by chromosomal instability and an increase in mutation and gene amplification frequencies) show a 3-fold increase in steady-state levels of hydrogen peroxide, but not superoxide. Furthermore, stable clones isolated from parallel studies showed significant increases in catalase and GPx (glutathione peroxidase) activity. Treatment of unstable cells with PEG-CAT (polyethylene glycol-conjugated catalase) reduced the mutation frequency and mutation rate in a dose-dependent fashion. In addition, inhibiting catalase activity in the stable clones using AT (3-aminotriazole) increased mutation frequency and rate. These results clearly demonstrate the causal relationship between chronic oxidative stress mediated by hydrogen peroxide and the mutator phenotype that persists for many generations following exposure of mammalian cells to ionizing radiation.

scholarly journals A Shuttle Vector System for Studying Ionizing Radiation-Induced Mutagenesis in Mammalian Cells.

1993 ◽  
Vol 34 (2) ◽  
pp. 148-156
Author(s):  
CHIDORI MURAISO ◽  
JOHN S. MUDGETT ◽  
HIROMICHI MATSUDAIRA ◽  
GARY F. STRNISTE
Keyword(s):  
Ionizing Radiation ◽  
Mammalian Cells ◽  
Shuttle Vector ◽  
Vector System ◽  
Radiation Induced ◽  
Induced Mutagenesis

scholarly journals Molecular markers of ionizing radiation-induced gene mutations in mammalian cells.

1996 ◽  
Vol 104 (suppl 3) ◽  
pp. 675-678 ◽  
Author(s):  
A W Hsie ◽  
R C Porter ◽  
Z Xu ◽  
Y Yu ◽  
J Sun ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Ionizing Radiation ◽  
Mammalian Cells ◽  
Gene Mutations ◽  
Radiation Induced

Radiation-induced surface decomposition

1983 ◽  
Vol 41 ◽  
pp. 368-369
Author(s):  
M. L. Knotek
Keyword(s):  
Ionizing Radiation ◽  
Atomic Structure ◽  
Chemical Bonding ◽  
Neutral Species ◽  
Radiation Induced ◽  
Physical And Chemical ◽  
Surface Decomposition ◽  
The Relationship ◽  
Electron And Photon ◽  
Surface Bond

Modern surface analysis is based largely upon the use of ionizing radiation to probe the electronic and atomic structure of the surfaces physical and chemical makeup. In many of these studies the ionizing radiation used as the primary probe is found to induce changes in the structure and makeup of the surface, especially when electrons are employed. A number of techniques employ the phenomenon of radiation induced desorption as a means of probing the nature of the surface bond. These include Electron- and Photon-Stimulated Desorption (ESD and PSD) which measure desorbed ionic and neutral species as they leave the surface after the surface has been excited by some incident ionizing particle. There has recently been a great deal of activity in determining the relationship between the nature of chemical bonding and its susceptibility to radiation damage.

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