Microbial Activity in the Forestomach of Free-Living Macropodid Marsupials - Comparisons With Laboratory Studies

1988 ◽  
Vol 36 (4) ◽  
pp. 383 ◽  
Author(s):  
DW Dellow ◽  
ID Hume ◽  
RTJ Clarke ◽  
T Bauchop
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Bacterial Flora ◽  
Laboratory Studies ◽  
Free Living ◽  
Fermentation Products ◽  
In The Wild ◽  
Ciliate Protozoa

Parameters of fermentative digestion were measured in five species of macropodid marsupials shot while feeding in the wild. These included details of microbiota, fermentation products (volatile fatty acids, gas, ammonia) and forestomach digesta pH. Ciliate protozoa and fungi, similar to anaerobic rumen fungi, were present in the forestomach of all species except Thylogale thetis. The bacterial flora was complex and numbers were similar to those in the ruminant forestomach. The forestomach gas contained more methane than found previously in captive macropodids, and in Wallabia bicolor hydrogen was present at 10- 11% of total gas. The pH of forestomach digesta was 5.7-6.7, indicative of animals actively feeding. Comparisons of stomach fill, ammonia and volatile fatty acid (VFA) concentrations and molar proportions, and rates of VFA production in the forestomach and hindgut, indicated that conclusions on digestive function in macropodids derived from studies on captive animals are generally applicable to free-living macropodids. The main differences probably lie in greater levels of feed intake in the field, and in greater opportunity for free-living macropodids to select from a more heterogeneous diet.

Rates of production of volatile fatty acids in the rumen. V. Evaluation of fodders in terms of volatile fatty acid produced in the rumen of the sheep

1967 ◽  
Vol 18 (4) ◽  
pp. 625 ◽  
Author(s):  
FV Gray ◽  
RA Weller ◽  
AF Pilgrim ◽  
GB Jones
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Night Time ◽  
Digestible Energy ◽  
Significant Difference ◽  
Freely Moving

l(a). Volatile fatty acid (VFA) production (moles/12 hr) by two sheep fed at 2-hourly intervals on a constant ration containing equal parts of wheaten hay and lucerne hay, showed no significant difference between the sheep or between day-time and night-time values. The precision with which production could be measured over a series of 12-hr periods is given. The quantities of VFA produced in the rumen on successive days varied considerably; the extent of this variation was similar to that occurring in the quantities of faeces passed. (b) Production remained much the same whether the sheep were fed at intervals of 1, 2, or 12 hr. (c) The energy of the VFA produced in the rumen was equivalent to about 54% of the digestible energy of the diet. 2. Similar amounts of VFA were produced from two different mixtures of wheaten hay and lucerne hay, and from lucerne hay alone. 3. A modification in the procedure for measuring VFA production was tested and found to be satisfactory. The necessary apparatus could be readily carried on the back of a freely moving sheep.

scholarly journals Activation of volatile fatty acids in bovine liver and rumen epithelium. Evidence for control by autoregulation

1973 ◽  
Vol 136 (2) ◽  
pp. 311-319 ◽  
Author(s):  
R. Ash ◽  
G. D. Baird
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Bovine Liver ◽  
Mitochondrial Matrix ◽  
Rumen Epithelium

1. The total capacities of homogenates of bovine liver and rumen epithelium to activate acetate, propionate and butyrate were determined. 2. Activating capacities were assayed by measuring the rate of formation of the corresponding CoA esters. The methods used for determining the concentrations of the CoA esters allowed the CoA esters of acetate, propionate and butyrate to be distinguished. It was thus possible to investigate the effect of the presence of a second volatile fatty acid on the rate at which a given volatile fatty acid was activated. 3. The propionate-activating capacity in rumen epithelium was decreased by about 87% in the presence of butyrate, the acetate-activating capacity in liver was decreased by about 55% in the presence of either propionate or butyrate, and the butyrate-activating capacity in liver was decreased by about 40–50% in the presence of propionate. 4. All three activating capacities in liver appeared to be located in the mitochondrial matrix and membrane. The three activating capacities had similar locations to each other in rumen epithelium as well, although in this case activity was more evenly divided between the mitochondria and the cytoplasm. 5. The relative activating capacities towards the volatile fatty acids in the two tissues, together with the ability of one volatile fatty acid to inhibit the activation of another volatile fatty acid, appear to ensure that butyrate is mainly metabolized in the rumen epithelium and that propionate is metabolized in the liver.

Studies on the production of volatile fatty acids from grass by rumen liquor in an artificial rumen: III. A note on the volatile fatty acid production from crude fibre and grass cellulose

1957 ◽  
Vol 49 (2) ◽  
pp. 180-183 ◽  
Author(s):  
A. John ◽  
G. Barnett ◽  
R. L. Reid
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Acid Production ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Relative Yield ◽  
Water Soluble ◽  
Cellulose Powder ◽  
Crude Fibre ◽  
Fatty Acid Production

1. The findings presented in two previous papers on the yields of volatile fatty acids, obtained by the action of rumen liquor in the artificial rumen, from fresh grass, dried grass and the water-soluble and water-insoluble separates of the latter, have been amplified by a consideration of the acids similarly obtained from specimens of chemically prepared crude fibre and cellulose, from four of the dried grass specimens.2. The proportions of different volatile fatty acids from grass crude fibre and grass cellulose resemble those obtained from cellulose powder, propionic acid being produced in greatest relative yield.3. A general review of these latter findings, in relation to those already presented, has been given.

Microbial metabolism in the forestomachs and the large intertine of sheep

1965 ◽  
Vol 16 (1) ◽  
pp. 77 ◽  
Author(s):  
VJ Williams
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Water Content ◽  
Large Intestine ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Wheat Grain ◽  
Maize Grain ◽  
The Individual

Sheep were fed on either lucerne chaff, high wheat grain, or high maize grain rations. Analyses were made on digesta from the rumen and large intestines of cannulated and slaughtered animals. The results for the large intestine showed that, when equal quantities of dry matter are given, the composition of the ration can affect the pH, the water content, and the total dry matter of the digesta; diet can also affect the total quantities of volatile fatty acid and ammonia in the organ.The proportions of the individual volatile fatty acids in the caecal fluid were not affected by the different diets fed in this experiment. The data did not suggest any consistent pattern in the pH or in the concentrations of ammonia and volatile fatty acid in the caecal fluid during the 24 hr between feeds.

Bio-augmentative volatile fatty acid production from waste activated sludge hydrolyzed at pH 12

RSC Advances ◽  
2015 ◽  
Vol 5 (62) ◽  
pp. 50033-50039 ◽  
Author(s):  
Xue Yang ◽  
Lei Wen ◽  
Xiang Liu ◽  
Si Chen ◽  
Yayi Wang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Activated Sludge ◽  
Acid Production ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Waste Activated Sludge ◽  
Soluble Complex ◽  
Fatty Acid Production ◽  
Hydrolysis Of

Although many pretreatment methods are employed to enhance the hydrolysis of waste activated sludge (WAS), the bioconversion of soluble complex substrates needs improvement to produce higher volatile fatty acids (VFAs).

scholarly journals The determination of volatile fatty acids in the caecum of the conscious rabbit

1976 ◽  
Vol 35 (3) ◽  
pp. 365-371 ◽  
Author(s):  
D. S. Parker ◽  
R. T. Mcmillan
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Acid Level ◽  
Volatile Fatty Acids ◽  
Fatty Acid Content ◽  
Diurnal Fluctuation ◽  
Fatty Acid Level ◽  
Conscious Rabbit

1. A method of obtaining dialysed samples from the caecum of the conscious rabbit is described.2. Values for total volatile fatty acid content and for molar proportions of individual volatile fatty acids in dialysate samples were in good agreement with those obtained from caecal material.3. The volatile fatty acid level in the caecum throughout the day was determined using two groups of animals, one group fed ad lib. and the other group on a restricted food intake. These results indicated a marked diurnal fluctuation in volatile fatty acid level in the caecum of rabbits fed once/d which was not evident in those fed ad lib.

scholarly journals Determination of volatile fatty acids clearance in intact ruminal digesta

2019 ◽  
Vol 14 (1) ◽  
pp. 8-17
Author(s):  
João Chrysostomo de Resende ◽  
João Luiz Pratti Daniel ◽  
André Rodrigues da Cunha Barreto-Vianna ◽  
Juliano Vogas Peixoto ◽  
Gregório Correia Guimarães ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Fractional Rate ◽  
Edta Solution ◽  
The Difference ◽  
Cr Concentration ◽  
Over Time

Cannulated cows were allocated to a cross-over design to evaluate the technique for determination of volatile fatty acid (VFA) clearance by infusion of marker solution into intact ruminal digesta, and verify the technique sensibility to the variation in the diet. Animals received diet of forage or forage plus concentrate and infusion of valeric acid and Cr-EDTA solution into intact or evacuated ruminal digesta. The total fractional rate of VFA clearance (FRVFAC) was estimated by the exponential decay of valerate over time. The passage FRVFAC to the omasum was assumed as the decay in ruminal Cr concentration and the absorption FRVFAC was estimated by the difference. The total FRVFAC and absorption did not differ between techniques. The passage FRVFAC, however, was lower when the marker solution was added into the evacuated digesta, probably reflecting the destabilization of the rumen environment during the evacuation and the largest volume of fluid observed in animals with evacuated digesta. In conclusion, the infusion of marker solution into intact digesta with homogenization performed by ruminal motility could be usable for the VFA ruminal clearance determination since it seems to cause lesser disturbance in the ruminal environment.

scholarly journals PENGARUH PERBEDAAN ARAS STARTER PADA FERMENTASI SABUT KELAPA TERHADAP KECERNAAN BAHAN PAKAN DAN PRODUKSI VOLATILE FATTY ACIDS SECARA IN VITRO

2020 ◽  
Vol 8 (2) ◽  
pp. 66
Author(s):  
Aulia Rahman ◽  
Sunarso Sunarso ◽  
B.I.M. Tampoebolon ◽  
L.K. Nuswantara
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids

Penelitian ini bertujuan untuk mengkaji pengaruh aras starter pada fermentasi sabut kelapa terhadap Kecernaan Bahan Kering (KcBK), Kecernaan Bahan Organik (KcBO) dan Volatile Fatty Acids (VFA) dengan metode in vitro. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL). Percobaan terdiri dari 4 perlakuan aras starter yaitu T0 (fermentasi sabut kelapa + 0% starter), T1 (fermentasi sabut kelapa + 2% starter), T2 (fermentasi sabut kelapa + 4% starter), dan T3 (fermentasi sabut kelapa + 6% starter) dengan 5 ulangan tiap perlakuan dan lama fermentasi 10 hari. Hasil penelitian menunjukkan sabut kelapa yang difermentasi dengan aras starter sampai 6% dapat meningkatkan (P<0,05) KcBK dan KcBO tetapi berpengaruh tidak nyata pada VFA. Rata-rata nilai KcBK dan KcBO pada perlakuan T0, T1, T2 dan T3 berturut-turut adalah KcBK 28,08%; 30,71%; 34,27%; 35,78% dan KcBO 28,73%; 31,81%; 35,21%; 36,88%. Rata-rata nilai produksi VFA pada perlakuan T0, T1, T2 dan T3 berturut-turut adalah 130 mM, 125 mM, 118 mM dan 120 mM. Simpulan penelitian adalah pemberian aras starter mampu meningkatkan KcBK dan KcBO dan produksi VFA memiliki kecenderungan menurun serta pemberian aras starter optimal pada level 4%. Kata kunci : Fermentasi, In vitro, Kecernaan, Sabut kelapa, Volatile fatty acid.

scholarly journals Ester-Producing Mechanism of Ethanol O-acyltransferaseEHT1Gene inPichia pastorisfrom Shanxi Aged Vinegar

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Jia Chen ◽  
Ruipeng Nan ◽  
Rufu Wang ◽  
Lixin Zhang ◽  
Junfeng Shi
Keyword(s):  
Fatty Acids ◽  
Esterase Activity ◽  
Volatile Fatty Acids ◽  
The Other ◽  
Fatty Acid Esters ◽  
Wild Type ◽  
Fermentation Products ◽  
Over Expression ◽  
In The Wild ◽  
Methyl Nonanoate

The ethanol O-acyltransferaseEHT1is an important element of key signaling pathways and is widely expressed in yeast strains. In this study, we investigated the expression ofEHT1 in the overexpression lines or knockout system ofPichia pastorisusing qRT-PCR and western blotting. The amount of total protein was determined using the Bradford method; the esterase activity was determined using p-nitrophenyl acetate as a substrate, and the production of volatile fatty acids in wild-type, knockout, and over-expression systems was detected using SPME GC-MS. The esterase activity ofEHT1-knockoutP. pastoriswas significantly lower than that in wild type (P<0.01), and the activities of esterase in threeEHT1-overexpressing strains—OE-1, OE-2, and OE-3—were significantly higher than those in wild type (P<0.01). In theEHT1-knockout strain products, the contents of nine volatile fatty acids were significantly lower than those in wild type (P<0.01), and the relative percentages of three fatty acids, methyl nonanoate, methyl decanoate, and ethyl caprate, were significantly lower than those in the other six species in the wild-type and knockout groups (P<0.05). The nine volatile fatty acids in the fermentation products of the overexpressedEHT1 gene were significantly higher than those in the wild-type group (P<0.01). The relative percentages of the three fatty acid esters, methyl nonanoate, methyl caprate, and ethyl caprate, were significantly higher than those in the other six species (P<0.05).EHT1 plays an important regulatory role in esterase activity and the production of medium-chain volatile fatty acids.

Plasma metabolite and volatile fatty acid concentrations in sheep divergently selected for predicted carcass lean content

1996 ◽  
Vol 1996 ◽  
pp. 107-107
Author(s):  
S.C. Bishop ◽  
J. Bracken ◽  
N.D. Cameron ◽  
K. Maclsaac ◽  
R.C. Noble ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Volatile Fatty Acids ◽  
Fat Metabolism ◽  
Blood Metabolites ◽  
Performance Tests ◽  
Correlated Responses ◽  
Selection Experiments ◽  
Plasma Metabolite

Blood measurements which can be taken on animals undergoing performance tests may be useful aids to selecting genetically superior animals. Possible useful measurements may be identified by correlated responses to selection, in selection experiments. This study investigates correlated responses in volatile fatty acids (VFAs) and blood metabolites, associated with growth and fat metabolism, in sheep selected for carcass lean content.

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