The Subspecific Biochemical Taxonomy of Antechinus minimus, A. swainsonii and Sminthopsis leucopus (Marsupialia: Dasyuridae)

1983 ◽  
Vol 31 (5) ◽  
pp. 753 ◽  
Author(s):  
AMA Smith

Electrophoretic variation in mainland and Tasmanian populations of Antechinus minimus, A. swainsonii and Sminthopsis leucopus was examined. The degree of genetic divergence observed among the trans-Bassian populations of each species corroborates the currently accepted subspecific rankings of A. minimus minimus and A. m. maritimus, A. swainsonii swainsonii and A. s. mimetes, and S. leucopus leucopus and S.l. ferruginifrons. The Flinders Island population of A. minimus was tentatively referred to the mainland subspecies, A. m. maritimus, on the basis of genetic similarity. Generally, levels of genetic divergence were found to be low when compared to those of similar taxonomic ranks in other animals.

2003 ◽  
Vol 60 (1) ◽  
pp. 115-118 ◽  
Author(s):  
Roberto Pedroso de Oliveira ◽  
Carlos Ivan Aguilar-Vildoso ◽  
Marcos Antônio Machado

Molecular markers have been used as tools in breading programs of sexual hybridation, allowing the genetic characterization of a large number of genotypes. The RADP markers are the most used since the employed techniques are simple and of low cost. To evaluate the genetic divergence among F1 hybrids of 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), this study analyses the variability and similarity of the hybrids among themselves and with their parents. Random Amplified Polimorfic DNA marker analysis, with 102 primers, were applied to a population composed of 94 hybrids and their parents. Multivariate genetic divergence analysis of the principal components and Tocher grouping were carried out only considering the polymorphic fragments. Genetic distances were calculated by the arithmetic complement of the Jaccard index. Bidimensional dispersion graphs among hybrid and parent distances and of the divergence analysis by principal components were constructed. High genetic similarity among Cravo and Pêra varieties and their hybrids was verified, showing a casual distribution from the hybrids in relation to the parents, but in intermediary positions. The principal component analysis showed little applicability in the study of hybrid genetic divergence. The hybrids and parents were classified in groups based on the genetic similarity, using the Tocher optimization method.


2021 ◽  
Vol 2 ◽  
Author(s):  
Malin Undin ◽  
Peter J. Lockhart ◽  
Simon F. K. Hills ◽  
Doug P. Armstrong ◽  
Isabel Castro

Reinforcement translocations are increasingly utilised in conservation with the goal of achieving genetic rescue. However, concerns regarding undesirable results, such as genetic homogenisation or replacement, are widespread. One factor influencing translocation outcomes is the rate at which the resident and the introduced individuals interbreed. Consequently, post-release mate choice is a key behaviour to consider in conservation planning. Here we studied mating, and its consequences for genomic admixture, in the North Island brown kiwi Apteryx mantelli population on Ponui Island which was founded by two translocation events over 50 years ago. The two source populations used are now recognised as belonging to two separate management units between which birds differ in size and are genetically differentiated. We examined the correlation between male and female morphometrics for 17 known pairs and quantified the relatedness of 20 pairs from this admixed population. In addition, we compared the genetic similarity and makeup of 106 Ponui Island birds, including 23 known pairs, to birds representing the source populations for the original translocations. We found no evidence for size-assortative mating. On the contrary, genomic SNP data suggested that kiwi of one feather did not flock together, meaning that mate choice resulted in pairing between individuals that were less related than expected by random chance. Furthermore, the birds in the current Ponui Island population were found to fall along a gradient of genomic composition consistent with non-clustered representation of the two parental genomes. These findings indicate potential for successful genetic rescue in future Apteryx reinforcement translocations, a potential that is currently under utilised due to restrictive translocation policies. In light of our findings, we suggest that reconsideration of these policies could render great benefits for the future diversity of this iconic genus in New Zealand.


2001 ◽  
Vol 32 (1) ◽  
pp. 99-106 ◽  
Author(s):  
P.C. Simões ◽  
M.M. Coelho ◽  
J.A. Quartau ◽  
M. Ribeiro

AbstractPortuguese populations from localities of sympatry and allopatry of Cicada orni Linnaeus and C. barbara (StÅl), a pair of closely related cicada species, were screened electrophoretically to clarify their genetic relationships. Thirteen enzyme systems coded by 19 presumptive loci were analysed. Seven loci were polymorphic on the 0.95 criterion. Data on within- and between-species variation, based on several genetic estimates (number of alleles per locus, amount of polymorphism, heterozygosity and genetic divergence) are presented. A dendrogram was constructed based on the Cavalli-Sforza & Edwards chord distance, using the UPGMA clustering method. Two groups were produced, one clustering the populations of C. orni and a second one clustering C. barbara. The former cicada species was much more variable genetically than C. barbara, this latter being a very homogeneous species. The high level of genetic similarity might suggest that C. barbara is a relatively recent immigrant to the Iberian Peninsula from its origin in North Africa. Alternatively, the present populations of C. barbara in Portugal might originate from small relict populations having survived the climatic cooling during the ice ages in a few warm refugia. Moreover, the present results suggest that reproductive isolation and therefore speciation in these cicadas may have occurred at a smaller genetic divergence level than in some other insects.


1977 ◽  
Vol 25 (4) ◽  
pp. 733 ◽  
Author(s):  
PG Johnston ◽  
GB Sharman

Geographic variation in potoroos (genus Potorous) from south-eastern Australia and Tasmania was investigated by electrophoretic and chromosomal techniques. Six of the 10 potoroo blood proteins examined showed electrophoretic variation and the genetic basis of three of these was established by breeding studies. Levels of genic heterozygosity were similar in Tasmanian and mainland populations. Coefficients of genetic similarity based on 10 loci indicated that potoroos from Tasmania and the Bass Strait islands are similar to each other but different from those of mainland Australia. No chromosomal variation was observed in potoroos examined from south-eastern Australia and Tasmania. Crosses between animals from these regions produced fertile offspring. It is concluded from this study and a related investigation on morphological variation that the genus Potorous should be separated into two species, P. platyops and P. tridactylus, with the further subdivision of the latter species into P.t. tridactylus from mainland Australia (including P. gilberti) and P.t. apicalis from Tasmania and the Bass Strait islands.


2012 ◽  
Vol 12 (1) ◽  
pp. 76-84 ◽  
Author(s):  
Marilene Santos de Lima ◽  
José Eustáquio de Souza Carneiro ◽  
Pedro Crescêncio Souza Carneiro ◽  
Camila Santana Pereira ◽  
Rogério Faria Vieira ◽  
...  

The purpose of this study was to characterize the genetic variability in 100 genotypes of the Active Germplasm Bank of common bean of the Federal University of Viçosa, by morphological descriptors, classify them in groups of genetic similarity and to identify the degree of relevance of descriptors of genetic divergence. The genotypes were evaluated based on 22 quantitative and qualitative morphological descriptors. The high-yielding genotypes V 7936, Gold Gate, LM 95103904, 1829 S 349 Venezuela, and PF 9029975, CNFC 9454 and Fe 732015, with upright growth, have potential for use as parents in common bean breeding programs. By genetic divergence analysis, the genotypes were clustered in eight groups of genetic dissimilarity. By methods of principal components, 9 of the 22 descriptors were eliminated, for being redundant or little variable, suggesting that 10-20 morphological descriptors can be used in studies of characterization of genetic variation.


Biologia ◽  
2015 ◽  
Vol 70 (1) ◽  
Author(s):  
Shijing Feng ◽  
Tuxi Yang ◽  
Xiao Li ◽  
Lv Chen ◽  
Zhenshan Liu ◽  
...  

AbstractChinese prickly ash, belonging to the genus Zanthoxylum L., has been one of the most important commerciallyexploited plants for its alimentary, industrial and medicinal applications. However, the breeding and promotion of Chinese prickly ash have been severely restricted due to its confusing classification. Therefore, we assessed genetic diversity and phylogenetic relationship among 45 Chinese prickly ash samples collected from 6 main cultivated regions using 11 ISSR primers. These eleven selected primers generated a total of 102 scorable bands ranging from 150 to 2000 bp, corresponding to an average of approximately 9.3 bands per primer. The percentage of polymorphic loci for all samples ranged from 75% to 100%, with an average of 84.3%. The genetic similarity coefficient across all samples varied from 0.460 to 0.919. Remarkably, UPGMA analysis showed that 45 samples were divided into six clusters with a genetic similarity of 0.7. The closest genetic relationship was observed between Dahongpao collected from Qin’an and Tianshui, and the greatest genetic divergence was found between Dahongpao collected from Hengshui and Jiuyeqing collected from Jiangjin. It could serve as a basis for identifying Chinese prickly ash cultivars, breeding new cultivars and protecting the Chinese prickly ash resources in main regions.


1979 ◽  
Vol 27 (3) ◽  
pp. 433 ◽  
Author(s):  
PG Johnston ◽  
GB Sharman

Geographic variation in island and mainland Australian populations of M. rufogriseus was investigated by electrophoretic, chromosomal and skull morphometric studies. Electrophoretic variation was observed at 9 of the 21 genetic loci examined. The mean proportion of polymorphic loci per population was 0.230 and individuals were on the average heterozygous at 6.5% of their loci. Coefficients of genetic similarity between populations ranged from 0.925 to 0.986, which is indicative of little genetic differentiation between populations. No chromosomal variation was observed in animals from the mainland, Tasmania and the Bass Strait islands. Preliminary data on breeding patterns suggest that females from King I. and Flinders I. are seasonal breeders, as has been reported for M. rufogriseus from Tasmania. It is concluded that M. rufogriseus should be divided into two subspecies: M. r. rufogriseus from Tasmania and the Bass Strait islands; M. r. banksianus from mainland Australia.


1988 ◽  
Vol 36 (1) ◽  
pp. 99 ◽  
Author(s):  
RL Close ◽  
S Ingleby ◽  
RAH Vanoorschot ◽  
AA Gooley ◽  
DA Briscoe ◽  
...  

Two rock-wallabies were captured in the Victoria Range of the Grampians, the first specimens obtained from Victoria for scientific study. Their chromosomes identified them as Petrogale penicillata and, although the animals appeared to be smaller than their nearest studied conspecifics from Jenolan Caves, N.S.W., 800 km to the north-east, analysis of blood proteins, red blood cell epitopes and parasites indicated little genetic divergence. This lack of differentiation is unusual in a genus in which, further north along the Great Dividing Range, nine chromosomally distinct forms occur within 1500 km. One animal was heterozygous for presence and absence of a major C-band on the second largest chromosome; chromosomes without this band have not been found in other mainland P. penicillata. No electrophoretic variation was detected at 23 genetic loci, even though one allele was unique among P. penicillata so far studied. Although only one extant colony was found, other disused sites were located 30 km further north. Despite the apparent low numbers of animals, there is some evidence that additional colonies may be found.


2021 ◽  
Vol 9 ◽  
Author(s):  
Xun Xu ◽  
Bao-Sheng Wang ◽  
Hui Yu

Understanding how intraspecies divergence results in speciation has great importance for our knowledge of evolutionary biology. Here we applied population genomics approaches to a fig wasp species (Valisia javana complex sp 1) to reveal its intraspecies differentiation and the underlying evolutionary dynamics. With re-sequencing data, we prove the Hainan Island population (DA) of sp1 genetically differ from the continental ones, then reveal the differed divergence pattern. DA has reduced SNP diversity but a higher proportion of population-specific structural variations (SVs), implying a restricted gene exchange. Based on SNPs, 32 differentiated islands containing 204 genes were detected, along with 1,532 population-specific SVs of DA overlapping 4,141 genes. The gene ontology (GO) enrichment analysis performed on differentiated islands linked to three significant GO terms on a basic metabolism process, with most of the genes failing to enrich. In contrast, population-specific SVs contributed more to the adaptation than the SNPs by linking to 59 terms that are crucial for wasp speciation, such as host reorganization and development regulation. In addition, the generalized dissimilarity modeling confirms the importance of environment difference on the genetic divergence within sp1. Hence, we assume the genetic divergence between DA and the continent due to not only the strait as a geographic barrier, but also adaptation. We reconstruct the demographic history within sp1. DA shares a similar population history with the nearby continental population, suggesting an incomplete divergence. Summarily, our results reveal how geographic barriers and adaptation both influence the genetic divergence at population-level, thereby increasing our knowledge on the potential speciation of non-model organisms.


Hereditas ◽  
2002 ◽  
Vol 136 (3) ◽  
pp. 240-246 ◽  
Author(s):  
P. M. O'GRADY ◽  
C. M. DURANDO ◽  
W. B. HEED ◽  
M. WASSERMAN ◽  
W. ETGES ◽  
...  

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