Variation in Sperm Head Morphology in the Australian Rodent Notomys alexis

1983 ◽  
Vol 31 (3) ◽  
pp. 313 ◽  
Author(s):  
WG Breed ◽  
V Sarafis
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Functional Significance ◽  
Sperm Head ◽  
Considerable Variability ◽  
Head Morphology ◽  
Scanning Electron

Scanning electron microscopy of the sperm head of the hopping-mouse indicates considerable variability in shape, much of which is probably due to the fragility of the acrosome. However, fluorescence microscopy of the sperm after staining with 4'-6-diamidino-2-phenylindole (DAPI) indicates some nuclear variability as well. Such polymorphism in sperm head morphology is unusual in mammals; its functional significance remains to be determined.

scholarly journals Removal of Composite Restoration from the Root Surface in the Cervical Region Using Er: YAG Laser and Drill—In Vitro Study

Materials ◽  
2020 ◽  
Vol 13 (13) ◽  
pp. 3027 ◽  
Author(s):  
Wojciech Zakrzewski ◽  
Maciej Dobrzynski ◽  
Piotr Kuropka ◽  
Jacek Matys ◽  
Malgorzata Malecka ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Composite Material ◽  
Fluorescence Microscopy ◽  
High Speed ◽  
Root Surface ◽  
Cervical Region ◽  
Tooth Surface ◽  
Yag Laser ◽  
Scanning Electron

Background: Recently, the defects of the tooth surface in the cervical region are often restored using composite filling materials. It should meet the needs of the patients regarding esthetics and material stability. The aim of the study was to analyze the tooth root surface at the cervical region after the removal of the composite filling material by means of the Erbium-doped Yttrium Aluminium Garnet (Er: YAG) laser or drill using the scanning electron microscopy (SEM) and fluorescence microscopy. Materials and Methods: For the purposes of this study, 14 premolar teeth (n = 14) were removed due to orthodontic reasons. The rectangular shape cavities with 3 mm in width and 1.5 mm in height were prepared with a 0.8 mm bur on high-speed contra-angle in the tooth surface just below cemento-enamel junction (CEJ) and filled with the composite material. The composite material was removed with the Er: YAG laser at a power of 3.4 W, energy 170 mJ, frequency 20 Hz, pulse duration 300 μs, tip diameter 0.8 mm, air/fluid cooling 3 mL/s, and time of irradiation: 6 sec, at a distance from teeth of 2 mm (G1 group, n = 7) or a high-speed contra-angle bur (G2 group, n = 7). After the removal of composite material, the surfaces of teeth were examined using the scanning electron microscopy (SEM) and fluorescence microscopy. Results: The Er: YAG irradiation allowed to remove completely the composite material from the tooth cavity. The study confirmed, that the ends of collagen fibers were only partially denatured after the Er: YAG laser application. Conclusion: It has been proved that using the Er: YAG laser is an effective and safe method of composite removal for the dentin surface.

Three-Dimensional Morphology of Microdamage in Peri-Screw Bone: A Scanning Electron Microscopy of Methylmethacrylate Cast Replica

2012 ◽  
Vol 18 (5) ◽  
pp. 1106-1111 ◽  
Author(s):  
Lei Wang ◽  
Jin Shao ◽  
Tingjun Ye ◽  
Lianfu Deng ◽  
Shijing Qiu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Light Microscopy ◽  
Three Dimensional ◽  
Basic Fuchsin ◽  
Bright Field ◽  
Different Types ◽  
Surrounding Bone ◽  
Scanning Electron

AbstractScrew implantation inevitably causes microdamage in surrounding bone. However, little is known about the detailed characteristics of microdamage in peri-screw bone. In this study, we developed a method to construct microdamage cast with methylmethacrylate (MMA) and observed the cast using scanning electron microscopy (SEM). In basic fuchsin stained bone sections observed by bright-field and fluorescence microscopy, diffuse damage, cross-hatched damage, and linear cracks were all presented in peri-screw bone. Using MMA casting/SEM method, we found numerous densely packed microcracks in the areas with diffuse damage. The osteocyte canaliculi and the microcracks consisting of diffuse damage had a similar diameter (or width), usually <0.5 μm, but their morphology was largely different. In the area with cross-hatched damage, the orientation of microcracks was similar to that in diffuse damage, but the number was significantly decreased. Many microcracks were thicker than 1 μm and associated with a rough surface. Large linear cracks (∼10 μm in diameter) occurred in different areas. Plenty of microcracks were present on the surface of some linear cracks. In conclusion, the MMA casting/SEM method can demonstrate the three-dimensional morphology of different types of microdamage, particularly the microcracks in diffuse damage, which are unable to be shown by light microscopy.

scholarly journals Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1407
Author(s):  
Ayoub Stelate ◽  
Eva Tihlaříková ◽  
Kateřina Schwarzerová ◽  
Vilém Neděla ◽  
Jan Petrášek
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Plasma Membrane ◽  
Fluorescence Microscopy ◽  
Environmental Scanning Electron Microscopy ◽  
Super Resolution ◽  
Convincing Evidence ◽  
Environmental Scanning ◽  
Fluorescence Light ◽  
Scanning Electron

Fluorescence light microscopy provided convincing evidence for the domain organization of plant plasma membrane (PM) proteins. Both peripheral and integral PM proteins show an inhomogeneous distribution within the PM. However, the size of PM nanodomains and protein clusters is too small to accurately determine their dimensions and nano-organization using routine confocal fluorescence microscopy and super-resolution methods. To overcome this limitation, we have developed a novel correlative light electron microscopy method (CLEM) using total internal reflection fluorescence microscopy (TIRFM) and advanced environmental scanning electron microscopy (A-ESEM). Using this technique, we determined the number of auxin efflux carriers from the PINFORMED (PIN) family (NtPIN3b-GFP) within PM nanodomains of tobacco cell PM ghosts. Protoplasts were attached to coverslips and immunostained with anti-GFP primary antibody and secondary antibody conjugated to fluorochrome and gold nanoparticles. After imaging the nanodomains within the PM with TIRFM, the samples were imaged with A-ESEM without further processing, and quantification of the average number of molecules within the nanodomain was performed. Without requiring any post-fixation and coating procedures, this method allows to study details of the organization of auxin carriers and other plant PM proteins.

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