Morphological Observations on the Leukocytes of the Koala, Phascolarctos cinereus (Goldfuss)

1982 ◽  
Vol 30 (2) ◽  
pp. 159 ◽  
Author(s):  
PJ Canfield ◽  
RK Dickens
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Light Microscope ◽  
Good Condition ◽  
Morphological Features ◽  
Phascolarctos Cinereus ◽  
Transmission Electron

Leukocytes from four mature koalas in good condition were examined with the light microscope (LM) and the transmission electron microscope (TEM). At the LM level, the leukocytes had morphological features similar to leukocytes of other marsupials and man. At the TEM level, the nuclear and cytoplasmic features of the leukocytes were compared to those of the leukocytes of man, and were found to be similar.

scholarly journals Comparison of pollen morphological structures of some taxa belonging to Asparagus L. and Fritillaria L. (Liliaceae) from Turkey

1970 ◽  
Vol 36 (2) ◽  
pp. 111-120 ◽  
Author(s):  
H Ozler ◽  
S Pehlivan
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Key Words ◽  
Transmission Electron Microscope ◽  
Light Microscope ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Exine Sculpture ◽  
Transmission Electron ◽  
Scanning Electron

Pollen grains of 20 taxa from two genera of the Liliaceae were examined and compared by LM (light microscope), SEM (scanning electron microscope) and pollens of four taxa were also examined with TEM (transmission electron microscope). Pollen grains shed as monads. They are monosulcate and ellipsoidal. Fritillaria crassifolia subsp. crassifolia Freyn & Smt. sometimes sheds the pollen as dyads. Exine is semitectate and the tectum is perforate. Columellae are simplicolumellate. Ectexine is thicker than endexine. Exine sculpture (ornamentation) is reticulate, reticulate-rugulate, rugulate and retipilate in Asparagus pollens and reticulate, suprareticulate, rugulate-reticulate and striate-reticulate in Fritillaria pollens. Sulcus extends from distal to proximal in some pollens of Asparagus and Fritillaria.   Key words: Asparagus, Fritillaria, Liliaceae, Pollen morphology DOI = 10.3329/bjb.v36i2.1498 Bangladesh J. Bot. 36(2): 111-120, 2007 (December)

Marine fungi from Seychelles. III. Aniptodera mangrovii sp.nov. from mangrove wood

1986 ◽  
Vol 64 (12) ◽  
pp. 2989-2992 ◽  
Author(s):  
Kevin D. Hyde ◽  
C. A. Farrant ◽  
E. B. Gareth Jones
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Light Microscope ◽  
Marine Fungi ◽  
Undescribed Species ◽  
Transmission Electron ◽  
Transmission Electron Microscope Micrographs ◽  
Mangrove Wood

Collections of higher marine fungi in Seychelles included an undescribed species of Aniptodera: A. mangrovii Hyde sp.nov. from driftwood and dead mangrove wood. This species differs from A. chesapeakensis in the size of the ascospores and in ascospore appendage morphology. Aniptodera mangrovii is described and illustrated by light microscope and scanning and transmission electron microscope micrographs.

Scanning Electron Miscroscopy of Marsilea Sperm

1975 ◽  
Vol 33 ◽  
pp. 574-575
Author(s):  
C. Y. Shih
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Light Microscope ◽  
Fine Detail ◽  
Transmission Electron ◽  
Whole Mount ◽  
Surface Detail ◽  
Scanning Electron

Although spermatogenesis in Marsilea has been studied initially by Sharp with the light microscope (LM), and subsequently by Mizukami and Gall with the transmission electron microscope (TEM), the overall organization of the sperm has not been recorded in detail with photomicrograph. The small size of the sperm (about 16 micrometers in diameter) makes it difficult to resolve the fine detail with the IM and the specimens are too thick to derive useful information by studying whole mount preparations with the TEM. The SEM with a magnification and resolution range which is intermediate between the LM and TEM can be useful to characterize the organization and surface detail of the Marsilea sperm.Marsilea is a water fern which has the appearance of a four leaf clover. The sporangia are enclosed within a hard, rounded sporocarp which is located at the base of the leaf. Germination of the mature sporocarp can be hastened in the laboratory by excising a small piece of the wall and immersing the sporocarp in water.

Comparison of Scanning Transmission and Backscattered Electron Imaging in the Sem, Using Heavy Metal Stained Sections of Biological Tissue

1976 ◽  
Vol 34 ◽  
pp. 322-323
Author(s):  
Phillip B. DeNee ◽  
Richard G. Frederickson
Keyword(s):  
Heavy Metal ◽  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Light Microscope ◽  
Biological Tissues ◽  
Scanning Transmission Electron Microscope ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Backscattered Electron ◽  
Better Than

The Transmission Electron Microscope (TEM) has been used extensively for the study of biological tissues in thin section (50-100 nm). For sectioned material greater than 100 nm, the Scanning Transmission Electron Microscope (STEM) and the High Voltage Electron Microscope (HVEM) have become the only alternatives for the study of these tissues at a resolution better than that obtained with the light microscope. Recently, it has been shown(1) that tissue stained with heavy metal can be studied in the Scanning Electron Microscope (SEM) by Backscattered Electron (BSE) imaging to give results similar to those obtained with the TEM. Because BSE imaging is a method complementary to STEM, it seemed worthwhile to compare the two techniques using the same specimens and beam conditions.Direct observation of the total specimen is possible with BSE imaging without interference by grid bars. Therefore, an improved perspective of tissue-totissue structural relationships can be obtained at a resolution significantly better than that of the light microscope.

scholarly journals Observing Traffic Within Cells

1997 ◽  
Vol 5 (8) ◽  
pp. 3-4
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Golgi Complex ◽  
Membrane Trafficking ◽  
Light Microscope ◽  
Intracellular Membrane ◽  
Transmission Electron ◽  
Static Images ◽  
Morphologic Data ◽  
Over Time

Ever since membranes could be resolved within cells, the pattern of intracellular membrane trafficking has been under investigation. Images made with the transmission electron microscope revealed numerous small vesicles that appeared to be shuttling between the endoplasmic rettculum (ER) and the Golgi complex. However, these static images offered no information as to the direction trie vesicles were moving and were quite inconclusive. Nevertheless, evidence built over time to suggest that unrefined proteins were moved in small vesicles from one intracellular compartment to another. Recently, a study by John Presley, Nelson Cole, Trina Schroer, Koret Hirschberg, Kristen Zaal, and Jennifer Lippincott- Schwartz with the light microscope has challenged this view. They not only presented convincing morphologic data in their published article, but they also posted some Quicktime movies on a Web site (http://dir.nichd.nih.gov/CBMB/pb1labob.html) that are even more convincing.

Micro-Measurement of the Virus-Infected Region Using NIS-Elements System

2010 ◽  
Vol 44-47 ◽  
pp. 1628-1632
Author(s):  
Hong Yi Yang ◽  
Xin Zhuang ◽  
Li Li Li ◽  
Zhi Wei Qin
Keyword(s):  
Image Processing ◽  
Electron Microscope ◽  
Alcian Blue ◽  
Transmission Electron Microscope ◽  
Light Microscope ◽  
Rt Pcr ◽  
Analysis Software ◽  
Average Area ◽  
Transmission Electron

A method was successfully developed for the measurement of the virus-infected region and distribution of virus. The system consists of a in situ PCR instrument, a light microscope, and an image processing and analysis software. Through developing a in situ RT-PCR system for location of a strawberry virus, the sections were observed on a Nikon light microscope by magnifying 400×. Alcian blue precipitate was observed in palisade tissues, and it indicated that the virus was located in the tissue. The area of the virus-infected region was measured by NIS-Elements software. At last, the distribution of virus in tissue and the area of infected region could be analyzed and accounted on the basis of the micro-measured results. The average area of forty virus-infected regions was 1.5×103 µm2, in addition, the ratio of the area of healthy region to virus-infected one was 3.4. The system achieved to measure quantitatively the virus-infected tissues dispensing with the complicated transmission electron microscope (TEM).

The Tem Evolved from a Lab Experiment Into an Analytical Tool

2001 ◽  
Vol 7 (S2) ◽  
pp. 526-527
Author(s):  
E. Van Cappellen ◽  
J. Ringnalda ◽  
Y.C. Wang ◽  
B. Bormans ◽  
T. Fliervoet
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
The Netherlands ◽  
Transmission Electron Microscope ◽  
Light Microscope ◽  
Analytical Tool ◽  
Ease Of Use ◽  
Transmission Electron ◽  
Significant Step ◽  
The Uk

It all started in Berlin in 1931 where Dr. Ernst Ruska built the first TEM with 2 magnetic lenses. Three years later a third lens was added and a resolution of l00nm was demonstrated, just enough to beat the light microscope by a factor of two. His achievements and vision was acknowledged and celebrated in 1986 when he was awarded the Nobel Prize for Physics. Philips’ commitment to electron microscopy dates back to the mid-1930s, when it collaborated in EM research programs with universities in the UK and the Netherlands. in 1949, the company introduced its first EM production unit, the EM 100 transmission electron microscope, which already was a significant step forward in terms of ease of use and reliability.In fifty years the TEM has come a long way; enumerating all the milestones would be too lengthy.

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