Anticoagulant Effects of Pindone in the Rabbit and Australian Bushtail Possum.

1993 ◽  
Vol 20 (3) ◽  
pp. 371 ◽  
Author(s):  
CT Eason ◽  
SE Jolly
Keyword(s):  
Toxic Effect ◽  
Prothrombin Time ◽  
Dose Group ◽  
Brushtail Possum ◽  
Trichosurus Vulpecula ◽  
Blood Samples ◽  
One Stage ◽  
The Mean ◽  
The One

The anticoagulant and toxic effect of the rodenticide pindone were compared in the rabbit and the Australian brushtail possum (Trichosurus vulpecula). Six possums and six rabbits were dosed orally with 25mg kg-1 pindone, and another five possums were dosed with 100mg kg-1 pindone. Blood samples were taken before dosing and 6 days after dosing. In rabbits, the mean haematocrit was reduced from 0.41 to 0.14, the one-stage prothrombin time increased from 7.1 to more than 120 s, and activated partial prothrombin time increased from 26.1 to more than 180 s. These parameters were unchanged in the possums that received 25mg kg-1. In possums that received 100mg kg-1 the one-stage prothrombin time increased from 41.9 to 80.0 s and the activated partial prothrombin time increased from 30.2 to 103.9 s. The haematocrit was unchanged. Half the rabbits died with extensive haemorrhaging within 6 days, but there was no haemorrhaging or mortality in the possums in either dose group.

Chicken prothrombin, thrombin, and fibrinogen

1961 ◽  
Vol 200 (5) ◽  
pp. 1013-1017 ◽  
Author(s):  
C. H. Bigland ◽  
D. C. Triantaphyllopoulos
Keyword(s):  
Prothrombin Time ◽  
Blood Coagulation ◽  
Thrombin Time ◽  
Bovine Thrombin ◽  
Chicken Brain ◽  
One Stage ◽  
Highly Sensitive ◽  
Avian Blood ◽  
The Mean ◽  
The One

Avian blood coagulation studies revealed the mean one-stage prothrombin time of plasma from 100 chickens, 10 weeks of age, as determined with chicken brain thromboplastin, to be 11.4 seconds, compared with 10–300 seconds found by other workers; the mean prothrombin content was 137% of human prothrombin levels, as determined by the one-stage prothrombin-time technique, but only 53% by the adsorption and elution technique used. Fibrinogen determinations on the same plasma revealed a mean of 346 mg/ 100 ml of plasma, compared with 250–400 for humans. Chicken thrombin was prepared which gave a mean thrombin time on the same, but buffered, plasma of 12 seconds. Coagulation of chicken plasma with chicken thrombin was found to be highly sensitive to variations in pH, being 12 seconds at pH 7.1, but lengthening to over 60 seconds at pH 7.7. Greatly increased clotting times of chicken plasma with bovine thrombin and with rabbit thromboplastin indicated evidence of species specificity.

The Reaction of Thiol Compounds with the Vitamin-K Dependent Clotting Factors

1969 ◽  
Vol 21 (03) ◽  
pp. 573-579 ◽  
Author(s):  
P Fantl
Keyword(s):  
Prothrombin Time ◽  
Vitamin K ◽  
Anaerobic Conditions ◽  
Clotting Factors ◽  
Thiol Compounds ◽  
Aerobic Conditions ◽  
One Stage ◽  
Factor Ii ◽  
Ph Dependent ◽  
The One

SummaryTreatment of human and dog oxalated plasma with 0.2 to 1.0 × 10−1 M 2.3-dithiopropanol (BAL) or dithiothreitol (DTT) at 2–4° C for 30 min results in the reduction of the vitamin-K dependent clotting factors II, VII, IX and X to the respective-SH derivatives. The reaction is pH dependent. Under aerobic conditions the delayed one stage prothrombin time can be partly reversed. Under anaerobic conditions a gradual prolongation of the one stage prothrombin time occurs without reversal.In very diluted plasma treated with the dithiols, prothrombin can be converted into thrombin if serum as source of active factors VII and X is added. In contrast SH factors VII, IX and X are inactive in the specific tests. Reoxidation to active factors II, VII, IX and X takes place during adsorption and elution of the SH derivatives. The experiments have indicated that not only factor II but also factors VII, IX and X have active-S-S-centres.

Two-Stage Procedure for the Quantitative Determination of Autoprothrombin III Concentration and Some Applications

1967 ◽  
Vol 18 (01/02) ◽  
pp. 198-210 ◽  
Author(s):  
Ronald S Reno ◽  
Walter H Seegers
Keyword(s):  
Prothrombin Time ◽  
Factor Vii ◽  
Two Stage ◽  
Pronounced Increase ◽  
One Stage ◽  
Assay Procedure ◽  
Bovine Plasma ◽  
The One ◽  
Autoprothrombin C

SummaryA two-stage assay procedure was developed for the determination of the autoprothrombin C titre which can be developed from prothrombin or autoprothrombin III containing solutions. The proenzyme is activated by Russell’s viper venom and the autoprothrombin C activity that appears is measured by its ability to shorten the partial thromboplastin time of bovine plasma.Using the assay, the autoprothrombin C titre was determined in the plasma of several species, as well as the percentage of it remaining in the serum from blood clotted in glass test tubes. Much autoprothrombin III remains in human serum. With sufficient thromboplastin it was completely utilized. Plasma from selected patients with coagulation disorders was assayed and only Stuart plasma was abnormal. In so-called factor VII, IX, and P.T.A. deficiency the autoprothrombin C titre and thrombin titre that could be developed was normal. In one case (prethrombin irregularity) practically no thrombin titre developed but the amount of autoprothrombin C which generated was in the normal range.Dogs were treated with Dicumarol and the autoprothrombin C titre that could be developed from their plasmas decreased until only traces could be detected. This coincided with a lowering of the thrombin titre that could be developed and a prolongation of the one-stage prothrombin time. While the Dicumarol was acting, the dogs were given an infusion of purified bovine prothrombin and the levels of autoprothrombin C, thrombin and one-stage prothrombin time were followed for several hours. The tests became normal immediately after the infusion and then went back to preinfusion levels over a period of 24 hrs.In other dogs the effect of Dicumarol was reversed by giving vitamin K1 intravenously. The effect of the vitamin was noticed as early as 20 min after administration.In response to vitamin K the most pronounced increase was with that portion of the prothrombin molecule which yields thrombin. The proportion of that protein with respect to the precursor of autoprothrombin C increased during the first hour and then started to go down and after 3 hrs was equal to the proportion normally found in plasma.

Comparison of the Thrombotest with the One-Stage Prothrombin Time

1961 ◽  
Vol 265 (26) ◽  
pp. 1286-1289 ◽  
Author(s):  
Armand J. Quick ◽  
Clara V. Hussey
Keyword(s):  
Prothrombin Time ◽  
One Stage ◽  
The One

INHERITED FACTOR-VII DEFECT IN A NEGRO FAMILY

PEDIATRICS ◽  
1961 ◽  
Vol 27 (2) ◽  
pp. 204-213
Author(s):  
Helen I. Glueck ◽  
James M. Sutherland
Keyword(s):  
Prothrombin Time ◽  
Factor Vii ◽  
Homozygous State ◽  
Control Value ◽  
One Stage ◽  
First Case ◽  
Severe Deficiency ◽  
Low Levels ◽  
The Difference ◽  
The One

A case of factor-VII deficiency of a congenital nature in a Negro male child has been reported. As far as can be determined, this is the first case reported in this race. The defect was detected at 6 hours of age. Prothrombin, as contrasted to factor VII, after initially low levels normally found in infants, rose to adult levels. The patient's one-stage prothrombin time has ranged between 25 to 35 second (normal 11 to 12 seconds). In spite of this, he has never shown any manifestations of hemorrhage. The patient's family was studied and the findings indicate that the patient's defect represented a homozygous state and that both parents with a less severe deficiency were heterozygous for the trait. The defect is an autosomal disorder directly inherited. It is clinically apparent and easily detected only in the homozygous state. The heterozygous state is characterized by a very slight prolongation of the one-stage prothrombin time, the difference from the control value being so minimal as to be overlooked. In one subject studied, an aunt of the propositus, the quantitative defect (42% of normal) could not be regularly detected by the usual methods. Only by using the plasma of the propositus as the test plasma, was the defect in her plasma detected, thus explaining the transmission of the trait to her offspring. These findings explain the difficulties previously encountered in understanding the inheritance of the disorder.

scholarly journals Prothrombin and the One-stage Prothrombin Time

BMJ ◽  
1955 ◽  
Vol 1 (4919) ◽  
pp. 934-937 ◽  
Author(s):  
A. J. Quick ◽  
C. V. Hussey
Keyword(s):  
Prothrombin Time ◽  
One Stage ◽  
The One

Effects of a Pneumatic Tube System on Routine and Novel Hematology and Coagulation Parameters in Healthy Volunteers

2007 ◽  
Vol 131 (2) ◽  
pp. 293-296
Author(s):  
Alexander Kratz ◽  
Raneem O. Salem ◽  
Elizabeth M. Van Cott
Keyword(s):  
Healthy Volunteers ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Activated Partial Thromboplastin Time ◽  
Tube System ◽  
Blood Samples ◽  
Pneumatic Tube ◽  
The Mean ◽  
Clinically Significant ◽  
Pneumatic Tube System

Abstract Context.—Technologic advances affecting analyzers used in clinical laboratories have changed the methods used to obtain many laboratory measurements, and many novel parameters are now available. The effects of specimen transport through a pneumatic tube system on laboratory results obtained with such modern instruments are unclear. Objective.—To determine the effects of sample transport through a pneumatic tube system on routine and novel hematology and coagulation parameters obtained on state-of-the-art analyzers. Design.—Paired blood samples from 33 healthy volunteers were either hand delivered to the clinical laboratory or transported through a pneumatic tube system. Results.—No statistically significant differences were observed for routine complete blood cell count and white cell differential parameters or markers of platelet activation, such as the mean platelet component, or of red cell fragmentation. When 2 donors who reported aspirin intake were excluded from the analysis, there was a statistically, but not clinically, significant impact of transport through the pneumatic tube system on the mean platelet component. There were no statistically significant differences for prothrombin time, activated partial thromboplastin time, waveform slopes for prothrombin time or activated partial thromboplastin time, fibrinogen, or fibrin monomers. Conclusions.—Although further study regarding the mean platelet component may be required, transport through a pneumatic tube system has no clinically significant effect on hematology and coagulation results obtained with certain modern instruments in blood samples from healthy volunteers.

Revised Standardization Method of the One-Stage Prothrombin Time

1987 ◽  
Vol 17 (6) ◽  
pp. 313-320
Author(s):  
Knut Korsbrekke ◽  
Ingebrigt Talstad
Keyword(s):  
Prothrombin Time ◽  
Standardization Method ◽  
One Stage ◽  
The One
Sign in / Sign up

Export Citation Format

Share Document