408. Liver gene expression in suckled postpartum beef cows maintained on moderate and improved subtropical pasture

2008 ◽  
Vol 20 (9) ◽  
pp. 88
Author(s):  
F. Samadi ◽  
H. M. Nadzir ◽  
N. J. Phillips ◽  
M. M. McGowan ◽  
SA Lehnert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Body Condition ◽  
Regulatory Element ◽  
Reproductive Function ◽  
Beef Cows ◽  
Binding Factor ◽  
Dairy Science ◽  
Sterol Regulatory Element ◽  
Hepatic Genes

The liver is the first organ to receive absorbed nutrients from the splanchnic vasculature1 which places the liver at an important juncture between nutrition, metabolic homeostasis and reproductive function. The best described component of this interface is secretion of IGF-1 by the liver and dependence of ovarian follicular maturation on hepatic-derived IGF-12. The aim in the present study was to ascertain the expression of hepatic genes associated with metabolic regulation in postpartum beef cows maintained on moderate or improved pasture, and with contrasting liveweight and body condition. Multiparous Droughtmaster cows (Bos taurus × Bos indicus) were placed on moderate (MP, n = 7) or improved (IP, n = 7) pasture at 6–7 months of gestation and remained on the treatments after calving. Liver biopsies were taken 6 weeks after calving and gene expression was determined by quantitative PCR using SYBR Green3. Week 6 represents attainment of the plateau in nutrient demand for lactation. Data were analysed by ANOVA using SAS STAT. One week after calving, IP cows had greater (P < 0.01) liveweight and body condition score (BCS) than MP cows (585 ± 12 kg and 528 ± 21 kg; 3.7 ± 0.2 BCS and 2.3 ± 0.2 BCS). IP cows had greater (P < 0.05) relative expression of hepatic genes associated with cholesterol biosynthesis (sterol regulatory element binding factor, SREBF2; peroxisome proliferators-activated receptor delta, PPARD), fatty acid biosynthesis (carnitine palmitoyl-transferase, CPT1A; acyl-CoA oxidase, ACOX1; fatty acid desaturase 1, FADS1; sterol regulatory element binding factor 1, SREBF1) and insulin signalling (hepatic insulin receptor substrate 1, IRS1). IP cows resumed cyclic ovarian function between 11–16 weeks postpartum and one MP cow had cycled by 16 weeks. The differences in expression of metabolic genes between MP and IP cows may have partly contributed to differences in reproductive function postpartum. (1) Drackley J et al. 2006 Journal of Dairy Science 89:1324–36 (2) Lucy MC 2000 Journal of Dairy Science 83:1635–47 (3) Loor JJ et al. 2006 Physiological Genomics 27:29–41

scholarly journals Lack of Sterol Regulatory Element Binding Factor-1c Imposes Glial Fatty Acid Utilization Leading to Peripheral Neuropathy

2015 ◽  
Vol 21 (4) ◽  
pp. 571-583 ◽  
Author(s):  
Gaia Cermenati ◽  
Matteo Audano ◽  
Silvia Giatti ◽  
Valentina Carozzi ◽  
Carla Porretta-Serapiglia ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Peripheral Neuropathy ◽  
Regulatory Element ◽  
Fatty Acid Utilization ◽  
Binding Factor ◽  
Sterol Regulatory Element

Impaired fatty acid synthesis affects immune cells activation: focus on sterol regulatory element binding factor-1c on T lymphocytes

2017 ◽  
Vol 263 ◽  
pp. e23
Author(s):  
Fabrizia Bonacina ◽  
Gaia Cermenati ◽  
Donatella Caruso ◽  
Alberico Luigi Catapano ◽  
Nico Mitro ◽  
...  
Keyword(s):  
Fatty Acid ◽  
T Lymphocytes ◽  
Fatty Acid Synthesis ◽  
Immune Cells ◽  
Regulatory Element ◽  
Acid Synthesis ◽  
Binding Factor ◽  
Sterol Regulatory Element ◽  
Impaired Fatty Acid

scholarly journals Insulin and Human Chorionic Gonadotropin Cause a Shift in the Balance of Sterol Regulatory Element-Binding Protein (SREBP) Isoforms Toward the SREBP-1c Isoform in Cultures of Human Granulosa Cells

2005 ◽  
Vol 90 (6) ◽  
pp. 3738-3746 ◽  
Author(s):  
Malcolm C. Richardson ◽  
Iain T. Cameron ◽  
Chantal D. Simonis ◽  
Madhab C. Das ◽  
Tessa E. Hodge ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chorionic Gonadotropin ◽  
Granulosa Cells ◽  
Human Chorionic Gonadotropin ◽  
Binding Protein ◽  
Regulatory Element ◽  
Human Granulosa Cells ◽  
Igf Binding ◽  
Sterol Regulatory Element ◽  
Igf Binding Protein

The isoforms of sterol regulatory element-binding proteins (SREBP) (1a, 1c, and 2) are key transcriptional regulators of lipid biosynthesis. We examined their regulation by gonadotropin and insulin in human granulosa cells. After removal of leukocytes, granulosa cells were exposed to hormonal additions for 16 h starting on d 2 of culture. Progesterone, lactate, and IGF binding protein-1 were measured in culture medium and cellular mRNA measured by competitive RT-PCR. Addition of human chorionic gonadotropin (hCG) (100 ng/ml) stimulated progesterone production (7.0-fold, P &lt; 0.001 vs. control), whereas lactate was increased by hCG (1.6-fold, P &lt; 0.001) and insulin (1.4-fold, P &lt; 0.001; 1000 ng/ml). Insulin decreased IGF binding protein-1 production by 85% (P &lt; 0.001). There were no significant effects on the expression of SREBP-1a but significant increases in mRNA for SREBP-1c with insulin (6.3-fold), hCG (10.4-fold) and in combination (15.2-fold; P &lt; 0.01 for all comparisons). No consistent effects on SREBP-2 were observed. The expression of mRNA for fatty acid synthase, a target gene for SREBP-1c, was increased by hCG (24-fold, P = 0.006) and insulin (19-fold, P = 0.024), which also increased the level of cellular, total fatty acid (1.34-fold; P = 0.03). Thus, hCG and insulin cause a switch toward expression of the SREBP-1c isoform with consequent effects on fatty acid synthesis. We suggest that high circulating insulin, associated with clinically defined insulin resistance, may up-regulate SREBP-1c expression in the ovary.

scholarly journals Sterol regulatory element binding transcription factor 1 (SREBF1) polymorphism and milk fatty acid composition

2013 ◽  
Vol 96 (4) ◽  
pp. 2605-2616 ◽  
Author(s):  
R.A. Nafikov ◽  
J.P. Schoonmaker ◽  
K.T. Korn ◽  
K. Noack ◽  
D.J. Garrick ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Regulatory Element ◽  
Milk Fatty Acid ◽  
Sterol Regulatory Element ◽  
Transcription Factor 1

Regulation of fatty acid synthase expression in breast cancer by sterol regulatory element binding protein-1c

2003 ◽  
Vol 282 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Y.u-A.n Yang ◽  
Patrice J. Morin ◽  
Wan Fang Han ◽  
Tinghua Chen ◽  
Daniel M. Bornman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fatty Acid ◽  
Fatty Acid Synthase ◽  
Binding Protein ◽  
Regulatory Element ◽  
Element Binding Protein ◽  
Sterol Regulatory Element

I. Molecular mechanism for polyunsaturated fatty acid regulation of gene transcription

2001 ◽  
Vol 281 (4) ◽  
pp. G865-G869 ◽  
Author(s):  
Steven D. Clarke
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Dna Binding ◽  
Regulatory Element ◽  
Health Benefit ◽  
Acid Oxidation ◽  
Nuclear Factor ◽  
Genes Encoding ◽  
Nuclear Abundance

This review addresses the hypothesis that polyunsaturated fatty acids (PUFA), particularly those of the n-3 family, play pivotal roles as “fuel partitioners” in that they direct fatty acids away from triglyceride storage and toward oxidation and they enhance glucose flux to glycogen. In doing this, PUFA may reduce the risk of enhanced cellular apoptosis associated with excessive cellular lipid accumulation. PUFA exert their beneficial effects by upregulating the expression of genes encoding proteins involved in fatty acid oxidation while simultaneously downregulating genes encoding proteins of lipid synthesis. PUFA govern oxidative gene expression by activating the transcription factor peroxisome proliferator-activated receptor-α. PUFA suppress lipogenic gene expression by reducing the nuclear abundance and DNA binding affinity of transcription factors responsible for imparting insulin and carbohydrate control to lipogenic and glycolytic genes. In particular, PUFA suppress the nuclear abundance and expression of sterol regulatory element binding protein-1 and reduce the DNA binding activities of nuclear factor Y, stimulatory protein 1, and possibly hepatic nuclear factor-4. Collectively, the studies discussed suggest that the fuel “repartitioning” and gene expression actions of PUFA should be considered among the criteria used in defining the dietary needs of n-6 and n-3 fatty acids and in establishing the dietary ratio of n-6 to n-3 fatty acids needed for optimum health benefit.

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