307. The effect of oxytocin on cell growth and steroid production in normal human prostate cells in vitro

2005 ◽  
Vol 17 (9) ◽  
pp. 131
Author(s):  
K. J. Hogarth ◽  
K. King ◽  
H. D. Nicholson
Keyword(s):  
Cell Growth ◽  
Epithelial Cells ◽  
Stromal Cell ◽  
Human Prostate ◽  
Steroid Production ◽  
Cell Numbers ◽  
Prostate Cells ◽  
Normal Human ◽  
Oxytocin Antagonist

Oxytocin (OT) is present in reproductive tissues of male mammals including human prostate tissue. OT increases prostatic muscle tone and prostatic growth. OT is increased in benign prostatic hyperplasia (BPH), an androgen dependent condition that develops with age. Dihydrotestosterone (DHT) is the active hormone in the prostate and is converted from testosterone by the enzyme 5 a reductase. Conversion has been shown to be augmented in the presence of OT. The aim of this study was to investigate the effect of oxytocin on cell growth and steroid production in cultured normal human prostate cells. Normal human prostate stromal and epithelial cells (Clonetics) were cultured with OT, oxytocin antagonist (OTA) or oxytocin/oxytocin antagonist combination (10 ng/mL, 1 ng/mL or 0.1 ng/mL) in media containing 10 nmol of testosterone. Media was changed daily over the 5 day growth period and frozen. Cell proliferation assay was performed at harvest on day 5 to ascertain cell numbers. Media from days 1, 3 and 5 were extracted and radioimmunoassayed for testosterone and DHT. OT increased stromal cell number in a dose dependent manner (P < 0.001). Treatment with OT or OTA had no significant effect on epithelial cell numbers. In stromal cell media from Day 1, DHT concentrations were higher in cells treated with OT than control cells (P < 0.05). By Day 5 the concentration of DHT was low in all treatment groups except OT (10 ng/mL). No effect of OT or OTA was seen on DHT concentrations of media from epithelial cells. OT may increase cell growth in prostate stromal cells but not epithelial cells grown in vitro. This effect may be related to the conversion of testosterone to DHT and DHT to its metabolites. These results demonstrate that OT may play a role in the regulation of cell growth, steroid production and steroid metabolism in the human prostate.

Effect of resveratrol and zinc on intracellular zinc status in normal human prostate epithelial cells

2009 ◽  
Vol 297 (3) ◽  
pp. C632-C644 ◽  
Author(s):  
Jun Jun Zhang ◽  
Min Wu ◽  
Norberta W. Schoene ◽  
Wen-Hsing Cheng ◽  
Thomas T. Y. Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Growth ◽  
Reactive Oxygen ◽  
Human Prostate ◽  
Oxygen Species ◽  
Zinc Status ◽  
Normal Human ◽  
Cellular Zinc ◽  
In Cells

To evaluate the influence of resveratrol on cellular zinc status, normal human prostate epithelial (NHPrE) cells were treated with resveratrol (0, 0.5, 1, 2.5, 5, and 10 μM) and zinc [0, 4, 16, and 32 μM, representing zinc-deficient (ZD), zinc-normal (ZN), zinc-adequate (ZA), and zinc-supplemented (ZS) conditions, respectively]. A progressive reduction in cell growth was observed in cells treated with increasing amounts of resveratrol (2.5–10 μM). Resveratrol at 5 and 10 μM resulted in a dramatic increase in cellular total zinc concentration, especially in ZS cells. Flow cytometry indicated that 10 μM resveratrol induced arrest of the cell cycle at the G2/M phase in association with the observed cell growth inhibition. Data from an in vitro experiment using zinquin as an indicator of intracellular free Zn(II) status demonstrated complex interactions between resveratrol and Zn(II). Fluorescence spectrofluorometry and fluorescence microscopic analyses revealed that intracellular free labile zinc was progressively elevated from nearly twofold in ZS cells with no resveratrol to multifold in ZA and ZS cells with 10 μM resveratrol compared with the corresponding ZN cells. Furthermore, increases in cellular zinc status were associated with elevated levels of reactive oxygen species and senescence, as evidenced by morphological and histochemical changes in cells treated with 2.5 or 10 μM resveratrol, especially in ZA and ZS cells. Taken together, the interaction between resveratrol and zinc in NHPrE cells increases total cellular zinc and intracellular free labile zinc status and, subsequently, reactive oxygen species production and senescence.

scholarly journals Lycopene Inhibits the Growth of Normal Human Prostate Epithelial Cells in Vitro

2003 ◽  
Vol 133 (11) ◽  
pp. 3356-3360 ◽  
Author(s):  
Ute C. Obermüller-Jevic ◽  
Estibaliz Olano-Martin ◽  
Ana M. Corbacho ◽  
Jason P. Eiserich ◽  
Albert van der Vliet ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Prostate ◽  
Prostate Epithelial Cells ◽  
Normal Human

HEPARIN-BINDING EPIDERMAL GROWTH FACTOR-LIKE GROWTH FACTOR IS AN AUTOCRINE MEDIATOR OF HUMAN PROSTATE STROMAL CELL GROWTH IN VITRO

2001 ◽  
Vol 165 (1) ◽  
pp. 284-288 ◽  
Author(s):  
JOSE LUIS DUQUE ◽  
ROSALYN M. ADAM ◽  
JOHN S. MULLEN ◽  
JIANQING LIN ◽  
JEROME P. RICHIE ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Growth ◽  
Epidermal Growth Factor ◽  
Stromal Cell ◽  
Human Prostate ◽  
Heparin Binding ◽  
Epidermal Growth

Stromal cell and human prostatic epithelial cell in-vitro co-coltures: Growth and morphology

1996 ◽  
Vol 63 (1_suppl) ◽  
pp. 65-68
Author(s):  
S. De Angeli ◽  
A. Fandella ◽  
C. Gatto ◽  
S. Buoro ◽  
C. Favretti ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Marked Reduction ◽  
Stromal Cell ◽  
Optical Microscope ◽  
Neutral Red ◽  
Cellular Growth ◽  
Murine Fibroblasts ◽  
Prostatic Epithelial Cell

A study was carried out on the effect of stroma-epithelium interaction on cellular growth and morphology in co-coltures of U285 prostatic epithelial cells with human prostatic and esophageal stromal cells and with murine fibroblasts of the 3T3-J2 line. The proliferation rate was determined by growth tests of neutral red and kenacid blue. Morphological observations were made under optical microscope on the same cultures used for the growth tests. Results highlighted a marked reduction in cellular growth in the co-cultures compared to control cultures, as well as the tendency of the stromal and epithelial cells to re-organise themselves in pseudo-acinous structures.

scholarly journals Androgen receptor localisation and turnover in human prostate epithelium treated with the antiandrogen, casodex

2000 ◽  
Vol 24 (3) ◽  
pp. 339-351 ◽  
Author(s):  
AS Waller ◽  
RM Sharrard ◽  
P Berthon ◽  
NJ Maitland
Keyword(s):  
Androgen Receptor ◽  
In Vitro Models ◽  
Human Prostate ◽  
Intracellular Targeting ◽  
Natural Ligand ◽  
Prostate Cells ◽  
Established Cell ◽  
Prostatic Epithelium ◽  
Prostate Epithelium

In vitro models of normal and malignant human prostate are currently limited to a few well established cell lines that, with a single exception (LNCaP), fail to express the androgen receptor (AR) - a common characteristic of prostatic epithelium grown in culture. To investigate the molecular mechanism of action of the non-steroidal antiandrogen Casodex (bicalutamide) against wild-type AR, we have established a transient AR expression model in non-tumorigenic prostate cells of both epithelial and mesenchymal origin. In this model, both dihydrotestosterone and Casodex can effectively transport the AR protein into the nucleus of prostate cells. Whereas the natural ligand, dihydrotestosterone, stabilises the receptor, the AR is rapidly degraded at a nuclear location when the transfected cells are treated with Casodex. In contrast, whereas the mutant AR in the LNCaP line is also degraded on Casodex treatment over the same time period, its intracellular targeting is defective.

scholarly journals Cigarette smoke condensate-induced transformation of normal human breast epithelial cells in vitro

Oncogene ◽  
2004 ◽  
Vol 23 (35) ◽  
pp. 5880-5889 ◽  
Author(s):  
Satya Narayan ◽  
Aruna S Jaiswal ◽  
Diana Kang ◽  
Pratima Srivastava ◽  
Gokul M Das ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cigarette Smoke ◽  
Cigarette Smoke Condensate ◽  
Human Breast ◽  
Breast Epithelial Cells ◽  
Normal Human Breast ◽  
Normal Human ◽  
Breast Epithelial ◽  
Human Breast Epithelial Cells

TNF-α-mediated apoptosis in normal human prostate epithelial cells and tumor cell lines

2004 ◽  
Vol 203 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Dharam P. Chopra ◽  
Raymond E. Menard ◽  
Jakub Januszewski ◽  
Raymond R. Mattingly
Keyword(s):  
Epithelial Cells ◽  
Tumor Cell ◽  
Cell Lines ◽  
Human Prostate ◽  
Tumor Cell Lines ◽  
Prostate Epithelial Cells ◽  
Tnf Α ◽  
Normal Human
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