scholarly journals 223.Ovarian pathology in mice following immunisation with recombinant murine cytomegalovirus expressing murine zona pellucida 3

2004 ◽  
Vol 16 (9) ◽  
pp. 223
Author(s):  
S. O'Leary ◽  
M. L. Lloyd ◽  
G. R. Shellam ◽  
S. Maddocks
Keyword(s):  
Zona Pellucida ◽  
Murine Cytomegalovirus ◽  
Research Centre ◽  
Post Inoculation ◽  
Leukocyte Infiltration ◽  
Cooperative Research ◽  
Cx43 Expression ◽  
Wild Mice ◽  
Primordial Follicles ◽  
Ovarian Pathology

Immunocontraception is a promising biological control for wild mice in Australia, having the potential to reduce the socioeconomic cost of plagues with minimal environmental impact. Inoculation of BALB/c mice with recombinant murine cytomegalovirus encoding murine zona pellucida antigen (mCMV-ZP3) confers total infertility characterised by depletion in ovarian tertiary follicles by Day 21 post inoculation followed by a progressive depletion in primordial follicles (1). The mechanisms underlying ovarian pathology are largely unknown but are likely to involve antibody mediated and cell mediated immune responses. The immune pathology may also be facilitated by acute responses involving antibody binding to ZP in growing follicles resulting in recruitment of inflammatory cells and oocyte destruction. The aim of this study was to investigate the effect of mCMV–ZP3 infection on leukocyte infiltration and expression of oocyte-derived signalling molecules in ovarian tissue. Fifteen BALB/c female mice were randomly allocated into three groups of 5 animals. Group one received an injection of PBS, group two and three received intraperitoneal inoculations of 2�×�104�p.f.u. of mCMV and mCMV-ZP3 respectively. Ovaries were retrieved at Day 7 post inoculation and one ovary from each mouse was sectioned for immunohistochemical analysis of resident leukocytes using mAb CD45 reactive with all leukocyte lineages. The other ovary was processed for real time quantitative RT-PCR analysis of growth and differentiation factor 9 (GDF-9) and connexin 43 (Cx43) expression. mCMV-ZP3 inoculation increased the abundance of ovarian leukocytes (P�=�0.08), significantly increased expression of Cx43 mRNA (p<0.05), but did not alter GDF-9 mRNA expression. These results suggest that changes in expression of ovarian regulators due to ZP3 immunisation begins early after recombinant MCMV infection in mice, and implicates leukocyte infiltration in the mechanism leading to permanent ovarian failure. Further experiments are underway to investigate the dynamics of leukocyte trafficking and expression of oocyte-derived signals as the course of infection progresses. This study is funded by the Cooperative Research Centre for Pest Animal Control. (1) Lloyd, M. L., et al. (2003). Biology of Reproduction 68(6): 2024-32.

284. Leukocyte trafficking in the ovary of mice immunised with recombinant murine cyclomegalovirus expressing murine zona pellucida 3

2005 ◽  
Vol 17 (9) ◽  
pp. 119
Author(s):  
S. O'Leary ◽  
M. L. Lloyd ◽  
G. R. Shellam ◽  
S. Maddocks
Keyword(s):  
T Cells ◽  
Zona Pellucida ◽  
Post Infection ◽  
Recombinant Virus ◽  
Murine Cytomegalovirus ◽  
Research Centre ◽  
Post Inoculation ◽  
Cooperative Research ◽  
Primordial Follicles

Inoculation of female BALB/c mice with recombinant murine cytomegalovirus encoding murine zona pellucida antigen (MCMV-ZP3) confers infertility characterised by depletion in ovarian tertiary follicles by day 21 post inoculation followed by a progressive depletion in primordial follicles.1 Cell mediated immune responses begin as early as day 10 post immunisation with MCMV-ZP32 with the recruitment of leukocytes before serum antibody can be clearly detected in mice. The physiological mechanisms leading to infertility in inoculated mice are being progressively delineated with the role of leukocyte subsets implicated in early pathological changes in ovarian architecture. The aim of this study was to investigate the effect of MCMV-ZP3 infection on leukocytes including T cells recruited into the ovary following infection with recombinant virus. Fifteen BALB/c female mice were randomly allocated into three groups of five animals at 6 weeks of age. Group one received an injection of PBS, group two and three received intraperitoneal inoculations of 2 × 104 pfu of MCMV and MCMV-ZP3 respectively. Ovaries were retrieved at day 10, 21 and 35 post inoculation and one ovary from each mouse was sectioned for immunohistochemical analysis of resident leukocytes using mAb CD45 reactive with all leukocyte lineages and mAb for CD4 and CD8 positive T cells. MCMV-ZP3 inoculation increased the abundance of ovarian leukocytes including CD4 and CD8 positive T cells for all time points post immunisation except for CD8 positive T cells 21 days post infection (Table 1). These results suggest that leukocytes, including T cells, are involved in causing early changes in the ovary post infection with MCMV-ZP3 that lead to the depletion of existing ovarian follicles leading to life long infertility in mice. Further experiments are underway to investigate the role of antibody and changes in leukocyte populations in the ovary as the course of infection with recombinant virus progresses. This study is funded by the Cooperative Research Centre for Pest Animal Control. (1)Lloyd ML, et al. (2003). Biol. Reprod. 68, 2024–32.(2)O’Leary S, et al. (2004). Reprod. Fertil. Devel. 16(Supplement), 77.

scholarly journals 222.Characterisation of the infertility effect induced by a recombinant murine cytomegalovirus expressing murine zona pellucida 3

2004 ◽  
Vol 16 (9) ◽  
pp. 222
Author(s):  
M. L. Lloyd ◽  
J. M. Papadimitriou ◽  
S. O'Leary ◽  
M. A. Lawson ◽  
G. R. Shellam
Keyword(s):  
Zona Pellucida ◽  
Recombinant Virus ◽  
Fertility Control ◽  
High Titre ◽  
Autoimmune Response ◽  
Murine Cytomegalovirus ◽  
Post Inoculation ◽  
Primary Role ◽  
Primordial Follicles

A recombinant mouse virus, murine cytomegalovirus, that has been engineered to encode the fertility antigen murine zona pellucida 3 (mZP3), is being developed for fertility control in mice. A single inoculation of the recombinant virus induces complete infertility in female BALB/c mice which persists for the breeding life of the animal. The extent of this autoimmune response was unexpected especially as the incorporation of mZP3 appears to have immunologically attenuated the growth of the recombinant virus. The histological features of the infection are an initial depletion in tertiary follicles by 21 days post inoculation followed by a progressive depletion of primordial follicles, leading to an almost complete absence of follicles by 150 days post-infection. High titre, long lasting, zona pellucida-specific antibody is present in infertile BALB/c mice although infertility has not been linked with either a critical titre or a dominant immunoglobulin isotype. However, our evidence suggests that anti-ZP3 antibody plays a primary role in infertility since antibodies are detected in vivo bound to the zona pellucida of ovaries from recombinant virus-infected mice, and passively transferred antibody from infected animals induces infertility in the absence of recombinant virus. In addition, an experiment in which immunoglobulin-deficient mice remained fertile after inoculation with the recombinant virus indicates that antibody is crucial for the immunocontraceptive effect to occur. Other immune mechanisms are also being explored.

scholarly journals Innovation Performance and Its Impact on Profitability Among Different Sectors in the Australian Construction Industry

2012 ◽  
Vol 6 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Mary Hardie ◽  
Graham Miller ◽  
Karen Manley ◽  
Stephen McFallan
Keyword(s):  
Construction Industry ◽  
Management Practices ◽  
Innovation Performance ◽  
Research Centre ◽  
Research Information ◽  
Cooperative Research ◽  
Building Research ◽  
Evaluation Systems ◽  
Formal Evaluation ◽  
Industry Sectors

The BRITE (Building Research Information Technology and Environment) project was established by the Australian Cooperative Research Centre for Construction Innovation to encourage innovation in the construction industry. While innovation is generally perceived to be broadly beneficial, there has been little formal study of its occurrence or impact in Australian construction or of the factors which foster an innovative atmosphere within an enterprise. In order to benchmark innovation performance, the BRITE project conducted a survey in 2004 into the nature, incidence and variety of technological and organisational innovations in various sectors of the industry. With some exceptions, the survey found that clients and consultants engaged in significantly higher levels of innovation than did suppliers, main contractors or trade contractors. Within the industry sectors those organisations classified as high innovators favoured the adoption of advanced management practices and had formal evaluation systems in place to judge their progress. They reported significant positive impacts on their profitability from innovation and can therefore provide instructive examples for the rest of the industry to follow.

CRC 'Regional Combinations' project — effects of genetics and growth paths on beef production and meat quality: experimental design, methods and measurements.

2005 ◽  
Vol 45 (8) ◽  
pp. 959 ◽  
Author(s):  
W. A. McKiernan ◽  
J. F. Wilkins ◽  
S. A. Barwick ◽  
G. D. Tudor ◽  
B. L. McIntyre ◽  
...  
Keyword(s):  
Meat Quality ◽  
Bos Taurus ◽  
Experimental Designs ◽  
Research Centre ◽  
Eating Quality ◽  
Beef Production ◽  
Cooperative Research ◽  
Beef Quality ◽  
Genetic Potential ◽  
And Storage

As a component of the second term of the Cooperative Research Centre (CRC) for Cattle and Beef Quality, a project to further test and validate the effects of varying nutritional growth paths pre-finishing and slaughter on cattle of varying genetic potential for meat yield and eating quality was designed and implemented. This project, ‘Regional Combinations’, was a multi-site experiment, using Bos taurus cattle generated at 4 locations across southern Australia. The design of imposing different growth paths between weaning and finishing on cattle with specific genetic potential is common across sites. Treatment and interaction effects on beef production and meat quality were examined within and across sites. This paper describes the experimental designs, generation of experimental cattle at the various sites and the measurements, collection and storage of the data for multi-site analyses.

Building on ‘Red Dirt’ Perspectives: What Counts as Important for Remote Education?

2015 ◽  
Vol 44 (2) ◽  
pp. 194-206 ◽  
Author(s):  
John Guenther ◽  
Samantha Disbray ◽  
Sam Osborne
Keyword(s):  
Educational Outcomes ◽  
Torres Strait Islander ◽  
Qualitative Data ◽  
Research Centre ◽  
Cooperative Research ◽  
Remote Communities ◽  
Remote Education ◽  
Community Expectations ◽  
Torres Strait ◽  
Indicators Of Success

The Remote Education Systems (RES) project within the Cooperative Research Centre for Remote Economic Participation (CRC-REP) has, over the last four years, gathered and analysed qualitative data directly from over 230 remote education stakeholders and from more than 700 others through surveys. The research was designed to answer four questions: (1) What is education for in remote Aboriginal and Torres Strait Islander communities?; (2) What defines ‘successful’ educational outcomes from the remote Aboriginal and Torres Strait Islander standpoint?; (3) How does teaching need to change in order to achieve ‘success’ as defined by the Aboriginal and Torres Strait Islander standpoint?; and (4) What would an effective education system in remote Australia look like? Based on this data, the paper reveals how perceptions differ for Aboriginal and Torres Strait Islander people from remote communities compared with people who come from elsewhere. The analysis points to the need for some alternative indicators of ‘success’ to match the aspirations of local people living in remote communities. It also points to the need for school and system responses that resonate with community expectations of education, and to develop narratives of aspiration and success alongside community views.

A review of genetic and non-genetic opportunities for manipulation of marbling

2004 ◽  
Vol 44 (7) ◽  
pp. 687 ◽  
Author(s):  
B. M. Bindon
Keyword(s):  
Vitamin A Deficiency ◽  
Genetic Correlations ◽  
Consumer Preference ◽  
High Energy ◽  
Water Soluble ◽  
Research Centre ◽  
Chemical Extraction ◽  
Eating Quality ◽  
Gene Marker ◽  
Cooperative Research

The biology of marbling is a considerable issue for the Australian beef industry. Measurement of the trait is still a concern: subjective assessment based on the degree of visual fat deposition and its distribution is the 'industry standard' and the basis for payment of marbling grades. Yet this measurement may be subject to operator error and is influenced by chiller temperature. Chemical extraction gives an unequivocal measure of all fat in the muscle (intramuscular fat percentage: IMF%) and has higher heritability and genetic variation than marble score; but does this mirror exactly what the trade regards as 'marbling'?Progeny test results from the Cooperative Research Centre (CRC) for Cattle and Beef Quality breeding projects provide improved understanding of breed and genetic effects on IMF% and marble score. Estimated breeding values (EBVs) for IMF% have been released to the industry for 7 breeds. Heritability estimates confirm that genetic progress will be faster when selection is based on IMF% rather than marble score. Genetic correlations of IMF% with growth, retail beef yield (RBY%), P8 fat, residual feed intake (RFI) and tenderness are now available to underpin selection indices. A favourable allele for marbling (TG5) on chromosome 14 has been identified by CSIRO/MLA as a direct gene marker for the trait. This is now being marketed as GeneSTAR marbling. Other favourable chromosomal regions are under investigation by the CRC.Nutritional manipulation of marbling remains problematic. It is accepted that high-energy grain diets achieve higher marbling than pasture diets. Within grain-based feedlot diets higher marbling is achieved with maize than barley, while barley diets in turn are better than sorghum. Steam flaking produces higher marbling than dry rolled grain and this effect is more marked with sorghum than maize. Beyond these establishments there are many uncertainties: experiments have examined the effects of diets with high protein; low protein; protected lipid; protected protein; added oil with and without calcium; vitamin A deficiency. None of these manipulations gave consistent improvement in marble score or IMF%. Commercial feedlots supplying Japanese B3/B4 markets may have successful dietary manipulations to enhance marbling but because of its proprietary nature the information is not normally available for scientific scrutiny.Japan is the only market for Australian beef where marbling is an important component of the market specification. There can be no doubt that marbling meets a special consumer preference in that niche market. In other markets scientific evidence for a link between marbling and beef tenderness or eating quality has been difficult to define (marbling is a key component of the USA grading scheme for primal cuts but Australia is not a big supplier to that market). In the domestic Meat Standards Australia market there is a trend for marbling to become more important as a consumer issue in 5-star products where higher order sensory attributes of beef come into play. Early meat science investigations concluded that beef flavour elements were water-soluble. This would exclude marbling fat as having a notable influence on flavour.Marbling remains the major determinant of carcass value in Australia's most valuable beef market. Research should continue to assist Australian producers to meet the specifications of that market with increased precision and reduced costs.

scholarly journals A Review of Murine Cytomegalovirus as a Model for Human Cytomegalovirus Disease—Do Mice Lie?

2020 ◽  
Vol 22 (1) ◽  
pp. 214
Author(s):  
Michelle A. Fisher ◽  
Megan L. Lloyd
Keyword(s):  
Human Cytomegalovirus ◽  
Murine Cytomegalovirus ◽  
Clinical Samples ◽  
Mouse Strains ◽  
Cytomegalovirus Disease ◽  
Wild Mice ◽  
Congenital Cytomegalovirus ◽  
Non Invasive ◽  
Species Specific ◽  
Disseminated Disease

Since murine cytomegalovirus (MCMV) was first described in 1954, it has been used to model human cytomegalovirus (HCMV) diseases. MCMV is a natural pathogen of mice that is present in wild mice populations and has been associated with diseases such as myocarditis. The species-specific nature of HCMV restricts most research to cell culture-based studies or to the investigation of non-invasive clinical samples, which may not be ideal for the study of disseminated disease. Initial MCMV research used a salivary gland-propagated virus administered via different routes of inoculation into a variety of mouse strains. This revealed that the genetic background of the laboratory mice affected the severity of disease and altered the extent of subsequent pathology. The advent of genetically modified mice and viruses has allowed new aspects of disease to be modeled and the opportunistic nature of HCMV infection to be confirmed. This review describes the different ways that MCMV has been used to model HCMV diseases and explores the continuing difficulty faced by researchers attempting to model HCMV congenital cytomegalovirus disease using the mouse model.

Current and future limits to automated 3D geological model construction

2021 ◽  
Author(s):  
Mark Jessell
Keyword(s):  
Structural Information ◽  
Mineral Exploration ◽  
Structural Data ◽  
Model Construction ◽  
Research Centre ◽  
Geological Model ◽  
Cooperative Research ◽  
Geological Modelling ◽  
3D Geological Model ◽  
Secondary Information

&lt;p&gt;In geological settings characterised by folded and faulted strata, and where good field data exist, we have been able to automate a large part of the 3D modelling process directly from the raw geological database (maps, bedding orientations and drillhole data). The automation is based upon the deconstruction of the geological maps and databases into positional, gradient and spatial and temporal topology information, and the combination of deconstructed data into augmented inputs for 3D geological modelling systems, notably LoopStructural and GemPy.&lt;/p&gt;&lt;p&gt;When we try to apply this approach to more complex terranes, such as greenstone belts, we come across two types of problem:&lt;/p&gt;&lt;ul&gt;&lt;li&gt;1) Insufficient structural data, since the more complexly deformed the geology, the more we need to rely on secondary structural information, such as fold axial traces and vergence to &amp;#8216;solve&amp;#8217; the structures. Unfortunately these types of data are not always stored in national geological databases. One approach to overcoming this is to analyse the simpler (i.e. bedding) data to try and estimate the secondary information automatically.&lt;/li&gt; &lt;/ul&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;&lt;ul&gt;&lt;li&gt;2) The available information is unsuited to the logic of the modelling system. Most modern modelling platforms assume the knowledge of a chronostratigraphic hierarchy, however, especially in more complexly deformed regions, a lithostratigraphy may be all that is available. Again a pre-processing of the map and stratigraphic information may be possible to overcome this problem.&lt;/li&gt; &lt;/ul&gt;&lt;p&gt;This presentation will highlight the progress that has been made, as well as the road-blocks to universal automated 3D geological model construction.&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;&lt;p&gt;We acknowledge the support of the MinEx CRC and the Loop: Enabling Stochastic 3D Geological Modelling (LP170100985) consortia. The work has been supported by the Mineral Exploration Cooperative Research Centre whose activities are funded by the Australian Government's Cooperative Research Centre Programme. This is MinEx CRC Document 2020/xxx.&lt;/p&gt;&lt;p&gt;&amp;#160;&lt;/p&gt;

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