The chemical nature of soil organic phosphorus. I. Inositol phosphates

Soil Research ◽  
1963 ◽  
Vol 1 (2) ◽  
pp. 203 ◽  
Author(s):  
DJ Cosgrove
Keyword(s):  
Soil Microorganisms ◽  
Chemical Nature ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Inositol Phosphates ◽  
Organic Phosphorus ◽  
Gradient Elution ◽  
Soil Organic Phosphorus ◽  
Derivatives Of ◽  
Elution Chromatography

Gradient elution chromatography, using a column of anion exchange resin, Dowex AG I-X8, has been used to separate and isolate constituents of the 'phytin' fraction of soil organic matter. In addition to the commonly occurring myoinositol hexaphosphate the presence of the corresponding derivatives of DL-inositol and scylloinositol was demonstrated. Of the lower inositol phosphates present pentaphosphates appeared to be the major constituents. The occurrence in nature of inositols, other than the myoinositol, as phosphorylated derivatives, has not previously been recorded. Present knowledge is insufficient to decide whether soil inositol phosphates are of plant origin or accumulated through the activities of soil microorganisms.

Gradient Elution Chromatography of the Acids in Concord and Other Grape Juices and Jellies

1965 ◽  
Vol 48 (3) ◽  
pp. 534-543
Author(s):  
David Jorysch ◽  
Seymour Marcus
Keyword(s):  
Citric Acid ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Free Acid ◽  
Grape Juice ◽  
Gradient Elution ◽  
Fraction Collector ◽  
Grape Juices ◽  
Dowex 1 ◽  
Elution Chromatography

Abstract The gradient elution technique was used to determine the acid profiles of grape juices and jellies prepared from their juices. Acids were separated by placing predetermined volumes of grape juices containing equal milliequivalents of free acid on columns of Dowex 1-X10 anion exchange resin in the formate form and eluting with formic acid in gradually increasing concentration. The eluates were collected in a fraction collector and evaporated to dryness on a waterbath, and the residues were titrated with standard alkali solution. The gradient elution chromatograms of the acids in Concord grape juice samples pressed in the laboratory differed only slightly from similar commercial samples. Grape juices other than Concord have similar acid patterns but show another acid peak. Grape jellies were liquefied and their acids determined by the method; the presence of added citric acid in these jellies is shown to affect the acidity patterns.

scholarly journals Tryptic phosphopeptides from whole casein. I. Preparation and analysis by fast protein liquid chromatography

1989 ◽  
Vol 56 (4) ◽  
pp. 603-611 ◽  
Author(s):  
Marcel A. Juillerat ◽  
Robert Baechler ◽  
Raphael Berrocal ◽  
Serge Chanton ◽  
Jean-Claude Scherz ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Gradient Elution ◽  
Fast Protein Liquid Chromatography ◽  
Parent Species ◽  
Net Charge ◽  
Phosphorylated Peptides ◽  
Salt Gradient

SummaryTryptic phosphopeptides were obtained from whole bovine casein by chromatography on the anion exchange resin QAE-Sephadex A 25. Salt gradient elution of the column allowed separation of non-phosphorylated peptides from phosphorylated species. The preparations obtained contained at least seven distinct phosphopeptides of which the following casein fragments were identified: αs1(43–58):2P, αs1(59–79): 5P, αs2(46–70): 4P, β(1–28): 4P, β(2–28): 4P, and β(33–48): 1P. Fast protein liquid chromatography (FPLC) on Mono Q HR 5/5 resin showed that the phosphopeptides were eluted in the same order as from the QAE-Sephadex resin. However, on the analytical column HR 5/5 the fragments αs1(59–79): 5P and β(2–28): 4P, having the same net charge under the conditions of chromatography, co-eluted, whereas they were at least partly separated on the preparative column HR 16/10. Following enzymic dephosphorylation, the peptides eluted at lower salt strength in the gradient. FPLC on Mono Q resin thus permitted dephosphorylation to be monitored and intermediates between the parent species and the fully dephosphorylated peptide to be identified.

Separation of Fixed Organic Acids in Table Sirups

1969 ◽  
Vol 52 (3) ◽  
pp. 559-564 ◽  
Author(s):  
Arthur R Johnson ◽  
Enrique Fernandez-Flores
Keyword(s):  
Organic Acids ◽  
Gradient Elution ◽  
Derivatives Of ◽  
Elution Chromatography ◽  
Gradient Elution Chromatography

Abstract A number of organic non-nitrogenous acids have been separated from table sirups and identified by gradient elution chromatography and by programmed temperature GLC analysis of the trisilyl derivatives of the acids.

The Gel Route to Yttrium Oxide

1988 ◽  
Vol 121 ◽  
Author(s):  
F. Ribot ◽  
C. Sanchez ◽  
J. Livage
Keyword(s):  
Rare Earth ◽  
Light Scattering ◽  
Anion Exchange ◽  
Yttrium Oxide ◽  
Close Agreement ◽  
Chemical Nature ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Sol Gel ◽  
Sol Gel Process

ABSTRACTSynthesis of pure Y2O3 Powders have been performed through the sol-gel process. Stable colloids in the range of 500 Å to 2000 Å have been obtained by hydroxylation of yttrium-aquo ions performed through an anion exchange resin.The chemical nature of these sols and gels investigated by GTA, DTA and EXAFS is in close agreement with Y(OH)3nH2O. Scattering experiments (S.A.N.S, S.A.X.S, light scattering) show that these colloids are made of anisotropie particles, that can be described as platelets 30 Å thick, 500 Å large and a few thousand Angstroms long.This synthesis can be extended to other rare earth colloids.

The Use of Amberlite IRA-410 for Preparation of Terminal Chlorodeoxy Sugars

1993 ◽  
Vol 58 (4) ◽  
pp. 902-908 ◽  
Author(s):  
Nada Vukojevic ◽  
Dušan Miljković ◽  
Mirjana Popsavin ◽  
Velimir Popsavin
Keyword(s):  
Anion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Nucleophilic Displacement ◽  
Selective Reagent ◽  
Derivatives Of

Anion-exchange resin Amberlite IRA-410 was found to be an effective and selective reagent for the nucleophilic displacement of primary tosyloxy (mesyloxy) groups in 3,5-disulfonates of xylofuranose derivatives I and III, as well as in 3,5,6-trisulfontes and 3,6-ditosyl derivatives of glucofuranose V, VII and IX. In all examined cases only the primary sulfonyloxy groups were replaced with chloride anions, whereupon the corresponding terminal chlorodeoxy derivatives were obtained in good yields.

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