Prevalence of human herpesvirus-8 among HIV-infected patients, intravenous drug users and the general population in Iran

Sexual Health ◽  
2016 ◽  
Vol 13 (3) ◽  
pp. 295 ◽  
Author(s):  
Rezvan Kakavand-Ghalehnoei ◽  
Zabihollah Shoja ◽  
Alireza Najafi ◽  
Mostafa Haji Mollahoseini ◽  
Shohreh Shahmahmoodi ◽  
...  
Keyword(s):  
General Population ◽  
Drug Users ◽  
Blood Donors ◽  
Human Herpesvirus ◽  
Intravenous Drug ◽  
Human Herpesvirus 8 ◽  
Intravenous Drug Users ◽  
Herpesvirus 8 ◽  
Sexual Behaviours ◽  
Polymerase Chain

Studies looking at the frequency of human herpesvirus-8 (HHV-8) among Iranian blood donors have produced conflicting results. The aim of this study was to investigate the prevalence of HHV-8 DNA by using polymerase chain reaction methods among 168 healthy individuals, 60 intravenous drug users and 100 HIV-infected patients from Iran. The prevalence of HHV-8 was significantly higher among intravenous drug users (13.3%) compared with the general population (3.6%; P = 0.017). The HHV-8 genome was mostly detected among intravenous drug users who displayed high-risk sexual behaviours. Moreover, the HHV-8 genome was also detected in 8% of HIV-infected patients. The present study findings support the likelihood that the transmission of HHV-8 is via a sexual route in the Iranian population.

scholarly journals Searching for Human Herpesvirus 8 Antibodies in Serum Samples from Patients Infected with Human Immunodeficiency Virus Type 1 and Blood Donors from São Paulo, Brazil

1999 ◽  
Vol 179 (6) ◽  
pp. 1591-1592 ◽  
Author(s):  
Adele Caterino‐de‐Araujo ◽  
Maria Luisa Calabrò ◽  
Elizabeth de los Santos‐Fortuna ◽  
Jamal Suleiman ◽  
Luigi Chieco‐Bianchi
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Blood Donors ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Serum Samples ◽  
Herpesvirus 8 ◽  
Immunodeficiency Virus

scholarly journals Human herpes virus 8 antibodies in HIV-positive patients in Surabaya, Indonesia

2020 ◽  
Author(s):  
Devi Oktafiani ◽  
Ni Luh Ayu Megasari ◽  
Elsa Fitriana ◽  
Nasronudin ◽  
Maria Inge Lusida ◽  
...  
Keyword(s):  
Drug Users ◽  
Sandwich Elisa ◽  
Human Herpesvirus ◽  
Intravenous Drug ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hiv Positive ◽  
Intravenous Drug Users ◽  
Serum Samples ◽  
Serological Tests ◽  
Human Herpes Virus 8

Background: Human herpesvirus 8 (HHV-8) infection is etiologically related to Kaposi’s sarcoma. Antibodies directed against HHV-8 can be detected in 80%–95% of HIV-seropositive patients with KS. HHV-8 serological tests have been done in several countries in Southeast Asia such as Malaysia, and Thailand however no serological data is available in Indonesia. This study was to examine the presence of HHV-8 antibodies in HIV-positive patients in Surabaya, Indonesia. Material and methods: Ninety-one serum samples were collected from HIV-positive patients in Surabaya, Indonesia. Human immunodeficiency virus-positive serum samples were collected from 10 homosexual men, 25 intravenous drug users (IVDUs) and 56 heterosexuals. Serums were then tested for the presence of HHV-8 antibody by using sandwich ELISA (Abbexa Ltd, Cambridge, UK). Results: The total of 91 HIV-infected were testing with antibodies to HHV-8 using enzyme-linked immunosorbent assay. Antibodies of HHV-8 were detected in 7/91 (7.7%) of the samples. According to a gender, six men (85.7%) and a women (14.3%) were positive of HHV-8 antibodies. No correlation regarding the gender and age from this study. The antibodies of HHV-8 was detected among intravenous drug users (IVDUs) men 5/7 (42.8%) and 2/7 (28.6%) from homosexual and heterosexual, respectively. Conclusion: This study found the presence of HHV-8 antibodies in 7.7% of patients in Surabaya, Indonesia. This finding was higher more than Southeast Asian countries. The patients with a positive result could suggest measures to prevent HHV-8 infection.

Detection and Titration of Human Herpesvirus-8–Specific Antibodies in Sera From Blood Donors, Acquired Immunodeficiency Syndrome Patients, and Kaposi's Sarcoma Patients Using a Whole Virus Enzyme-Linked Immunosorbent Assay

Blood ◽  
1998 ◽  
Vol 92 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Louise G. Chatlynne ◽  
William Lapps ◽  
Michael Handy ◽  
Yao Q. Huang ◽  
Rizwan Masood ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Kaposi's Sarcoma ◽  
Blood Donors ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Antibody Detection ◽  
Human Herpesvirus 8 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Herpesvirus 8

A human herpesvirus-8 (HHV-8) enzyme-linked immunosorbent assay (ELISA) with a whole virus lysate as antigen was developed and used to measure the seroprevalence rate and levels of IgG antibodies to HHV-8 in sera/plasma of various patient groups and blood donors. The virus antigen was prepared from the KS-1 cell line, which produces lytic virus, and therefore contains a broad array of viral proteins. Seroprevalence studies using this ELISA showed the following: 10 of 91 blood donors (11%) had an average HHV-8 antibody titer of 118; 67 of 72 (93%) classic Kaposi's sarcoma (KS) patients were positive with an average titer of 14,111; and 57 of 62 (92%) KS/human immunodeficiency virus (HIV) patients were positive with an average titer of 4,000. A study on a very limited number of serial serum samples from patients before and after diagnosis with KS showed highly elevated antibody titers to HHV-8 virus after KS lesions developed. Preliminary data show that 50% of the sera from HIV-1+ homosexual patients contain IgG antibodies to HHV-8 suggesting that this population is at high risk for developing KS. Antibody results correlated well with the confirmatory immunofluorescent assays (IFA) using KS-1 cells as the substrate. This HHV-8 IgG antibody detection ELISA is sensitive and specific and does not cross-react with Epstein-Barr virus (EBV) or other human herpesviruses. The results of this HHV-8 antibody survey suggest that this rapid ELISA assay can be used to screen large numbers of sera to find those at risk for developing KS.

scholarly journals Seroprevalence of Human Herpesvirus 8 and Hepatitis C Virus among Drug Users in Shanghai, China

Viruses ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 2519-2530 ◽  
Author(s):  
Tiejun Zhang ◽  
Ying Liu ◽  
Yuyan Zhang ◽  
Jun Wang ◽  
Veenu Minhas ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Drug Users ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8

High prevalence in Central Africa of blood donors who are potentially infectious for human herpesvirus 8

Transfusion ◽  
1998 ◽  
Vol 38 (8) ◽  
pp. 771-775 ◽  
Author(s):  
L Belec ◽  
N Cancre ◽  
MC Hallouin ◽  
J Morvan ◽  
A Mohamed ◽  
...  
Keyword(s):  
Blood Donors ◽  
Central Africa ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Herpesvirus 8 ◽  
High Prevalence

Human herpesvirus 8 is not detectable by polymerase chain reaction in angiosarcoma

1998 ◽  
Vol 138 (3) ◽  
pp. 546-547 ◽  
Author(s):  
Martínez‐Escribano ◽  
Del Pino Gil‐Mateo ◽  
Miquel ◽  
Ledesma ◽  
Aliaga
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Herpesvirus ◽  
Human Herpesvirus 8 ◽  
Chain Reaction ◽  
Herpesvirus 8 ◽  
Polymerase Chain
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