The prevalence of Trichomonas vaginalis detected by wet mount and polymerase chain reaction in Sydney women

Sexual Health ◽  
2013 ◽  
Vol 10 (4) ◽  
pp. 385 ◽  
Author(s):  
Ivy Kwon ◽  
Anna McNulty ◽  
Phillip Read
Keyword(s):  
Polymerase Chain Reaction ◽  
Trichomonas Vaginalis ◽  
Health Centre ◽  
Chain Reaction ◽  
Local Data ◽  
Cross Sectional ◽  
Wet Mount ◽  
Polymerase Chain ◽  
Sexually Transmissible Infection ◽  
Transmissible Infection

Objectives Although Trichomonas vaginalis (TV) has a low profile in urban Australia, local data has estimated the prevalence in women to be 10 times higher when using polymerase chain reaction (PCR) versus wet mount microscopy (4.8% v. 0.4%). Our aim was to determine the prevalence of TV in Sydney women using both wet mount and PCR. Methods: A cross-sectional study was conducted of women requiring sexually transmissible infection screening at the Sydney Sexual Health Centre. Vaginal swabs were examined for TV using PCR and wet mount microscopy. Results: In total, 781 of 1263 eligible women were tested; 3 out of 781 tested positive by PCR and 1 out of 781 by wet mount, giving a prevalence of 0.38% (95% confidence interval (CI): 0.14–1.12%) and 0.13% (95% CI: 0.03–0.71%) respectively. There was not enough power to compare PCR and wet mount. Conclusions: The results of this analysis indicate that in our female urban population, TV is a very rare sexually transmissible infection,with 0.38% prevalence, and routine screening by PCR is not indicated.

scholarly journals Detection ofTrichomonas vaginalisUsing the Polymerase Chain Reaction in Pregnant and Non-Pregnant Women

1994 ◽  
Vol 2 (1) ◽  
pp. 16-19 ◽  
Author(s):  
J. Jeremias ◽  
D. Draper ◽  
M. Ziegert ◽  
W. Jones ◽  
S. Inglis ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pregnant Women ◽  
Trichomonas Vaginalis ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Vaginal Infections ◽  
Wet Mount ◽  
Polymerase Chain ◽  
Asymptomatic Women ◽  
Culture Positive

Objective:Trichomonas vaginalisvaginal infections are often both asymptomatic and difficult to detect by current methods. We evaluated the ability of a newly developed polymerase chain reaction (PCR) assay to identifyT. vaginalisin vaginal samples from pregnant and non-pregnant women.Methods:In the 1st study, we compared the prevalence ofT. vaginalisdetection by PCR and culture using Diamond's medium in 52 women with symptoms of vaginal infection. In the 2nd study,T. vaginaliswas detected using PCR and wet mount microscopy in 131 asymptomatic pregnant women.Results:Among the women with symptoms of vaginitis, 7 (13.5%) were PCR-positive forT. vaginalis. Six of the PCR-positive women, but none of the PCR-negative women, were culture-positive for this organism. All but 1 of the women with candidal vaginitis or bacterial vaginosis were PCR-negative for T.vaginalis. Among the asymptomatic pregnant women, all of whom were negative forT. vaginalisby wet mount, l0 (7.6%) were PCR-positive forT. vaginalis.Conclusions:PCR offers a rapid and sensitive alternative to culture and microscopy for the detection ofT. vaginalisvaginal infections in both symptomatic and asymptomatic women.

Accuracy of real-time polymerase chain reaction to detect Schistosoma mansoni – infected individuals from an endemic area with low parasite loads

Parasitology ◽  
2020 ◽  
Vol 147 (10) ◽  
pp. 1140-1148
Author(s):  
Fernanda do Carmo Magalhães ◽  
Samira Diniz Resende ◽  
Carolina Senra ◽  
Carlos Graeff-Teixeira ◽  
Martin Johannes Enk ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Schistosoma Mansoni ◽  
Real Time ◽  
Diagnostic Methods ◽  
Control Measures ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Intestinal Schistosomiasis ◽  
Polymerase Chain

AbstractDue to the efforts to control schistosomiasis transmission in tropical countries, a large proportion of individuals from endemic areas present low parasite loads, which hinders diagnosis of intestinal schistosomiasis by the Kato-Katz (KK) method. Therefore, the development of more sensitive diagnostic methods is essential for efficient control measures. The aim was to evaluate the accuracy of a real-time polymerase chain reaction (RT-PCR) to detect Schistosoma mansoni DNA in fecal samples of individuals with low parasite loads. A cross-sectional population-based study was conducted in a rural community (n = 257) in Brazil. POC-CCA® was performed in urine and feces were used for RT-PCR. In addition, fecal exams were completed by 18 KK slides, saline gradient and Helmintex techniques. The combined results of the three parasitological tests detected schistosome eggs in 118 participants (45.9%) and composed the consolidated reference standard (CRS). By RT-PCR, 117 out of 215 tested samples were positive, showing 91.4% sensitivity, 80.2% specificity and good concordance with the CRS (kappa = 0.71). RT-PCR identified 86.9% of the individuals eliminating less than 12 eggs/g of feces, demonstrating much better performance than POC-CCA® (50.8%). Our results showed that RT-PCR is a valuable alternative for the diagnosis of intestinal schistosomiasis in individuals with very low parasite loads.

A cross-sectional study of Leishmania spp. infection in clinically healthy dogs with polymerase chain reaction and serology in Greece

2002 ◽  
Vol 109 (1-2) ◽  
pp. 19-27 ◽  
Author(s):  
Leonidas S Leontides ◽  
Manolis N Saridomichelakis ◽  
Charalambos Billinis ◽  
Vasilios Kontos ◽  
Alexander F Koutinas ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Leishmania Spp ◽  
Healthy Dogs ◽  
Polymerase Chain

scholarly journals Diagnosis of Trichomonas vaginalis using real-time polymerase chain reaction (PCR) among women at Institut Pasteur of Cte dIvoire

2018 ◽  
Vol 12 (43) ◽  
pp. 988-993
Author(s):  
T. Blavo-Kouamé ◽  
K. E. Angora ◽  
A. Yéo ◽  
A. Ouattara ◽  
A. Ira-Bonouman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Trichomonas Vaginalis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals A new single run polymerase chain reaction assay for cyclosporiasis in immunocompromised patients

2021 ◽  
Vol 7 (3) ◽  
pp. 207-212
Author(s):  
Rakesh Singh ◽  
Manish Katiyar ◽  
Reena Gulati ◽  
Sreejith Parameswaran ◽  
Abdoul Hamide ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Tertiary Care ◽  
Cost Effective ◽  
People Living With Hiv ◽  
Immunocompromised Patients ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Cross Sectional ◽  
Stool Samples ◽  
Polymerase Chain

causes human intestinal cyclosporiasis. It is more common in the immunocompromised patients and mainly seen in people living with HIV/AIDS (PLHA), post-renal transplant (PRT) patients and immunocompromised children (IC). Diagnostic microscopy for the oocysts of the parasite is less sensitive, requiring examination of multiple stool samples. Here we developed a new single run polymerase chain reaction (PCR) assay for the detection of and it was used to know the hospital based prevalence of cyclosporiasis. A cross-sectional study was conducted from June 2016 to October 2020 in a tertiary care teaching hospital. A new single run amplification PCR-based diagnostic assay was developed for . Stool samples were collected from 121 PLHA, 135 PRT and 79 immunocompromised children (IC) other than PLHA and PRT. All stool samples were examined for the presence of oocysts as well as tested with new PCR assay. Modified Ziehl-Neelsen staining of the concentrated stool smear did not reveal oocysts of species in any stool specimen. However, new PCR assay detected in 2 stool specimens – one from a PLHA patient and another from a PRT patient, giving a prevalence of 0.6% (2/335), 0.8% (1/121) in PLHA and 0.7% (1/135) in PRT. It was not detected in IC. Cyclosporiasis is infrequent in southern part of India. The new single run PCR assay developed by us is simple and cost effective molecular assay for the detection of .

scholarly journals Uji Diagnostik C-Reactive Protein pada Pneumonia Bakteri Komunitas Anak

Sari Pediatri ◽  
2016 ◽  
Vol 17 (5) ◽  
pp. 391
Author(s):  
Ikhsan Marzony ◽  
Finny Fitry Yani ◽  
Efrida Efrida
Keyword(s):  
Polymerase Chain Reaction ◽  
C Reactive Protein ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Polymerase Chain

Latar belakang. Polymerase chain reaction (PCR) merupakan teknik untuk menentukan agen penyebab pneumonia dengan sensitivitas dan spesifisitas yang tinggi, tetapi harganya mahal dan tidak tersedia di semua tempat. C-reactive protein (CRP) juga dapat digunakan untuk memprediksi infeksi bakteri dan memiliki keunggulan yang lain, yakni harganya yang murah dan hampir tersedia di semua laboratorium.Tujuan. Mengetahui sensitivitas, spesifisitas, nilai prediksi positif, dan nilai prediksi negatif CRP pada pneumonia bakteri komunitas anak.Metode. Penelitian cross sectional pada 62 subjek di Bagian Ilmu Kesehatan Anak RS. Dr. M. Djamil Padang dari Desember 2013 sampai Oktober 2014. Subjek dipilih dengan teknik konsekutif. Dilakukan uji CRP dan teknik PCR sebagai baku emas. Duapuluh tiga sampel diekslusi karena menderita penyakit jantung bawaan (7), sepsis (6), telah mendapatkan antibiotik (4), dan orang tua menolak pemeriksaan (6) sehingga total sampel menjadi 39 anak.Hasil. Sebagian besar subjek penelitian adalah laki-laki (59%) dan kelompok umur terbanyak 2-11 bulan (48,7%). Sensitivitas CRP dengan cut off point 8 mg/L adalah 51,6%, spesifisitas 75%, nilai prediksi positif 88,8%, nilai prediksi negatif 28,6%. Pada cut off point 10 mg/L, sensitivitas CRP adalah 41,9%, spesifisitas 87,5%, nilai prediksi positif 92,9%, nilai prediksi negatif 28%.Kesimpulan. C-reactive protein tidak dapat digunakan sebagai uji tapis terhadap pneumonia bakteri komunitas anak.

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