Evolutionary relationships of Heimioporus and Boletellus (Boletales), with an emphasis on Australian taxa including new species and new combinations in Aureoboletus, Hemileccinum and Xerocomus

2015 ◽  
Vol 28 (1) ◽  
pp. 1 ◽  
Author(s):  
Roy E. Halling ◽  
Nigel Fechner ◽  
Mitchell Nuhn ◽  
Todd Osmundson ◽  
Kasem Soytong ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
New Combinations ◽  
Additional Species ◽  
Translation Elongation ◽  
Spore Ornamentation ◽  
Translation Elongation Factor 1Α

Boletellus and Heimioporus, two genera of Boletaceae with ornamented basidiospores, are shown to be distinct genera on the basis of phylogenetic analyses of nuclear ribosomal large-subunit and translation elongation-factor 1α DNA sequences. Comparison of spore ornamentation type – longitudinally ribbed in Boletellus v. punctate, alveolate-reticulate in Heimioporus – are further evidence for distinction. Analyses of multiple accessions from the Americas, Asia and Australia support the monophyly of Heimioporus and a ‘core Boletellus’ clade, containing the type species, B. ananas (M.A.Curt.) Murrill, and approximately seven additional species. Tests of alternative phylogenetic topologies could not reject monophyly of a more inclusive group containing the core Boletellus clade and six other species. Heimioporus australis Fechner & Halling, H. cooloolae Fechner & Halling, Boletellus deceptivus Halling & Fechner, B. reminiscens Halling & Fechner and B. sinapipes Fechner, K.Syme, R.Rob. & Halling are described as new species. Phylogenetic analyses also support the following new combinations: Aureoboletus projectellus (Murrill) Halling, A. mirabilis (Murrill) Halling, Hemileccinum subglabripes (Peck) Halling and the new name, Xerocomus tenax Nuhn & Halling.

Affinities of the Boletus chromapes group to Royoungia and the description of two new genera, Harrya and Australopilus

2012 ◽  
Vol 25 (6) ◽  
pp. 418 ◽  
Author(s):  
Roy E. Halling ◽  
Mitchell Nuhn ◽  
Todd Osmundson ◽  
Nigel Fechner ◽  
James M. Trappe ◽  
...  
Keyword(s):  
New Genus ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Sister Group ◽  
Molecular Data ◽  
Translation Elongation ◽  
Spore Deposit ◽  
Large Subunit Rdna ◽  
Translation Elongation Factor 1Α

Harrya is described as a new genus of Boletaceae to accommodate Boletus chromapes, a pink-capped bolete with a finely scabrous stipe adorned with pink scabers, a chrome yellow base and a reddish-brown spore deposit. Phylogenetic analyses of large-subunit rDNA and translation elongation factor 1α confirmed Harrya as a unique generic lineage with two species, one of which is newly described (H. atriceps). Some Chinese taxa were recently placed in a separate genus, Zangia, supported by both morphology and molecular data. Multiple accessions from Queensland, Australia, support the synonymy of at least three species in a separate Australian clade in the new genus, Australopilus. The truffle-like Royoungia is also supported as a separate lineage in this clade of boletes. Even though it lacks stipe characters, it possesses the deep, bright yellow to orange pigments in the peridium. Additional collections from Zambia and Thailand represent independent lineages of uncertain phylogenetic placement in the Chromapes complex, but sampling is insufficient for formal description of new species. Specimens from Java referable to Tylopilus pernanus appear to be a sister group of the Harrya lineage.

Baorangia duplicatopora (Boletaceae, Boletales), a new bolete from tropical China

Phytotaxa ◽  
2021 ◽  
Vol 508 (1) ◽  
Author(s):  
XU ZHANG ◽  
ZHI-QUN LIANG ◽  
SHUAI JIANG ◽  
CHANG XU ◽  
XIN-HUA FU ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Its Region ◽  
Tropical Region ◽  
Translation Elongation ◽  
Line Drawings ◽  
Microscopic Features ◽  
Tropical China

Baorangia duplicatopora is described as a new species from Hainan Province, a tropical region of China. It is morphologically characterized by large to very large basidiomata with a dull rose red, rose pink to purplish red pileus, compound pores, pileus context near hymenophore and stipe context staining blue when injured, a red stipe, and cheilocystidia wider than those of other Baorangia species. Phylogenetic analyses of DNA sequences from part of the 28S gene, the nuc rDNA internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α gene (TEF1) also confirmed that B. duplicatopora forms an independent lineage within Baorangia. Detailed descriptions, color photographs of fresh basidiomata, and line drawings of microscopic features of the new species are presented. A key to species of Baorangia in the world is also provided.

Tylopilus callainus, a new species with a sea-green color change of hymenophore and context from the south of China

Phytotaxa ◽  
2018 ◽  
Vol 343 (3) ◽  
pp. 269 ◽  
Author(s):  
ZHI-QUN LIANG ◽  
MING-SHENG SU ◽  
SHUAI JIANG ◽  
DENG HONG ◽  
NIAN-KAI ZENG
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Color Change ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
The South ◽  
Translation Elongation ◽  
Green Color ◽  
Line Drawings ◽  
A New Species

Tylopilus callainus (Boletaceae, Boletales) is described as a new species from the south of China. It is morphologically characterized by a brown pileus tinged with sea-green, a sea-green color change of hymenophore and context when injured, a stipe with sea-green color on the upper part, and a trichodermal pileipellis with slightly inflated hyphae. Phylogenetic analyses of DNA sequences from partial 28S regions, the nuc rDNA internal transcribed spacer (ITS) and the translation elongation factor 1-α gene (TEF1) also confirm that T. callainus forms an independent lineage within Tylopilus s. str. Consequently, a detailed description, color photos of fresh basidiomata and line-drawings of microstructures are presented.

scholarly journals Differential patterns of ophiostomatoid fungal communities associated with three sympatric Tomicus species infesting pines in south-western China, with a description of four new species

MycoKeys ◽  
2019 ◽  
Vol 50 ◽  
pp. 93-133 ◽  
Author(s):  
Wang HuiMin ◽  
Zheng Wang ◽  
Fu Liu ◽  
Cheng Xu Wu ◽  
Su Fang Zhang ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Bark Beetles ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Forest Health ◽  
Elongation Factor ◽  
Distinct Species ◽  
Western China ◽  
Ophiostomatoid Fungi ◽  
Additional Species

Bark beetles and their associated fungi, which cause forest decline and sometimes high mortality in large areas around the world, are of increasing concern in terms of forest health. ThreeTomicusspp. (T.brevipilosus,T.minorandT.yunnanensis) infect branches and trunks ofPinusyunnanensisandP.kesiyain Yunnan Province, in south-western China.Tomicusspp. are well known as vectors of ophiostomatoid fungi and their co-occurrence could result in serious ecological and economic impact on local forest ecosystems. Nonetheless, knowledge about their diversity, ecology, including pathogenicity and potential economic importance is still quite rudimentary. Therefore, an extensive survey of ophiostomatoid fungi associated with theseTomicusspecies infestingP.yunnanensisandP.kesiyawas carried out in Yunnan. Seven hundred and seventy-two strains of ophiostomatoid fungi were isolated from the adult beetles and their galleries. The strains were identified based on comparisons of multiple DNA sequences, including the nuclear ribosomal large subunit (LSU) region, the internal transcribed spacer regions 1 and 2, together with the intervening 5.8S gene (ITS) and the partial genes of β-tubulin (TUB2), elongation factor 1α (TEF1-α) and calmodulin (CAL). Phylogenetic analyses were performed using maximum parsimony (MP) as well as maximum likelihood (ML). Combinations of culture features, morphological characters and temperature-dependent growth rates were also employed for species identification. Eleven species belonging to five genera were identified. These included six known species,Esteyavermicola,Leptographiumyunnanense,Ophiostomabrevipilosi,O.canum,O.minusandO.tingensand four novel taxa, described asGraphilbumanningense,O.aggregatum,SporothrixpseudoabietinaandS.macroconidia. A residual strain was left unidentified asOphiostomasp. 1. The overall ophiostomatoid community was by far dominated by three species, representing 87.3% of the total isolates; in decreasing order, these wereO.canum,O.brevipilosiandO.minus. Furthermore, the ophiostomatoid community of each beetle, although harbouring a diversity of ophiostomatoid species, was differentially dominated by a single fungal species;Ophiostomacanumwas preferentially associated with and dominated the ophiostomatoid community ofT.minor, whereasO.brevipilosiandO.minuswere exclusively associated with and dominated the ophiostomatoid communities ofT.brevipilosusandT.yunnanensis, respectively. Eight additional species, representing the remaining 12.7% of the total isolates, were marginal or sporadic. These results suggested that sympatricTomicuspopulations are dominated by distinct species showing some level of specificity or even exclusivity.

scholarly journals More pieces to a huge puzzle: Two new Escovopsis species from fungus gardens of attine ants

MycoKeys ◽  
2019 ◽  
Vol 46 ◽  
pp. 97-118 ◽  
Author(s):  
Quimi Vidaurre Montoya ◽  
Maria Jesus Sutta Martiarena ◽  
Danilo Augusto Polezel ◽  
Sérgio Kakazu ◽  
Andre Rodrigues
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Phylogenetic Position ◽  
High Genetic Diversity ◽  
Translation Elongation ◽  
Attine Ants ◽  
Elongation Factor 1 Alpha ◽  
Fungus Growing Ants

Escovopsis (Ascomycota: Hypocreales, Hypocreaceae) is the only known parasite of the mutualistic fungi cultivated by fungus-growing ants (Formicidae: Myrmicinae: Attini: Attina, the “attines”). Despite its ecological role, the taxonomy and systematics of Escovopsis have been poorly addressed. Here, based on morphological and phylogenetic analyses with three molecular markers (internal transcribed spacer, large subunit ribosomal RNA and the translation elongation factor 1-alpha), we describe Escovopsisclavatus and E.multiformis as new species isolated from fungus gardens of Apterostigma ant species. Our analysis shows that E.clavatus and E.multiformis belong to the most derived Escovopsis clade, whose main character is the presence of conidiophores with vesicles. Nevertheless, the most outstanding feature of both new species is the presence of a swollen region in the central hypha of the conidiophore named swollen cell, which is absent in all previously described Escovopsis species. The less derived Escovopsis clades lack vesicles and their phylogenetic position within the Hypocreaceae still remains unclear. Considering the high genetic diversity in Escovopsis, the description of these new species adds barely two pieces to a huge taxonomic puzzle; however, this discovery is an important piece for building the systematics of this group of fungi.

scholarly journals New species of Retiboletus (Boletales, Boletaceae) from China based on morphological and molecular data

MycoKeys ◽  
2020 ◽  
Vol 67 ◽  
pp. 33-44
Author(s):  
Hai-Ying Liu ◽  
Yan-Chun Li ◽  
Tolgor Bau
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Large Subunit ◽  
Elongation Factor ◽  
Molecular Data ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Line Drawings ◽  
Morphological And Molecular Data ◽  
Elongation Factor 1

Species of the genus Retiboletus in China were investigated based on morphology and phylogenetic analysis of DNA sequences from the nuclear ribosomal large subunit (nrLSU) and the translation elongation factor 1-α gene (TEF1-α). Nine species were recovered from China, including two new and seven known species. The new species, namely Retiboletus ater and R. sinogriseus, from southwestern and northeastern China respectively, are documented and illustrated in this paper. Retiboletus ater is morphologically characterized by its black to grayish black pileus, white to grayish hymenophore, black to blackish stipe and white to grayish white context. Retiboletus sinogriseus is morphologically characterized by its brown to grayish-brown pileus, yellow to grayish-yellow hymenophore, pale yellow to brownish stipe and yellow to brownish-yellow context. Descriptions and line drawings of these two novel species and their comparisons with allied taxa are presented.

scholarly journals A morphological and molecular survey of Neoconidiobolus reveals a new species and two new combinations

2021 ◽  
Author(s):  
Yong Nie ◽  
Zi-Min Wang ◽  
Xiao-Yong Liu ◽  
Bo Huang
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
New Combinations ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
A New Species

Abstract The genus Neoconidiobolus was recently established to accommodate all members of the Conidiobolus subgenus Conidiobolus. Based on mitochondrial small subunit (mtSSU), nuclear large subunit (nucLSU) of rDNA and translation elongation factor 1-alpha (TEF1), this study further resolved the genus Neoconidiobolus into three clades, with three new taxa being added. They are N. kunyushanensis B. Huang & Y. Nie, sp. nov., N. lamprauges (Drechsler) B. Huang & Y. Nie, comb. nov., and N. nanodes (Drechsler) B. Huang & Y. Nie, comb. nov. Meanwhile, a morphologial comparison among species in the three clades and a key to the species of the genus Neoconidiobolus are provided herein.

A new species of Chlorociboria (Helotiales, Ascomycota) on herbaceous stems from China

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 111
Author(s):  
HUAN-DI ZHENG ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Central China ◽  
Nuclear Ribosomal Dna ◽  
Light Blue ◽  
Ectal Excipulum ◽  
A New Species

A new species, namely Chlorociboria herbicola, is discovered on herbaceous stems in central China. Morphologically, the new fungus is distinctive by the combination of light blue-green apothecia, rectangular cells in ectal excipulum, and elongate-ellipsoidal ascospores with rounded ends. Phylogenetic analyses of the internal transcribed spacer and large subunit of nuclear ribosomal DNA sequences confirm its ascription in Chlorociboria and distinction from the known species of the genus.

Generic classification of some more hyphomycetes with solitary conidia borne on phialides

1998 ◽  
Vol 76 (9) ◽  
pp. 1570-1583 ◽  
Author(s):  
W Gams ◽  
K O'Donnell ◽  
H -J Schroers ◽  
M Christensen
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
28S Rdna ◽  
New Combination ◽  
Rdna Sequences ◽  
28S Rdna Sequences ◽  
Three New Species ◽  
Morphological Species

Unlike most phialide-producing fungi that liberate a multiplicity of conidia from each conidiogenous cell, only single conidia are formed on phialide-like conidiogenous cells in Aphanocladium, Verticimonosporium, and some species of Sibirina. A group of isolates obtained from soil of native Artemisia tridentata (sagebrush) grassland in Wyoming and from desert soil in Iraq is compared with these genera and classified as a fourth genus, Stanjemonium, honouring Stanley J. Hughes. Phylogenetic analyses of partial nuclear small- (18S) and large-subunit (28S) rDNA sequences indicate that Stanjemonium spp. form a monophyletic group with Emericellopsis. Sequences from the nuclear 18S and 28S rDNA were too conserved to resolve morphological species of Stanjemonium; however, phylogenetic analysis of b-tubulin and translation elongation factor 1a gene exons and introns resolved all species distinguished morphologically. Numerous conidiogenous cells or denticles are scattered along the cells of aerial hyphae in Aphanocladium and Stanjemonium spp., very rapidly collapsing into denticles in the former, somewhat more persistent and leaving broad scars in the latter. In Cladobotryum-Sibirina and Verticimonosporium spp., conidiogenous cells are discrete in terminal and intercalary whorls; phialides of the latter taxon are particularly swollen. The taxonomy of Aphanocladium is not yet resolved. Two species are recognized in Verticimonosporium. Three new species of Stanjemonium are described, and one new combination from Aphanocladium is proposed, along with one new species of Cladobotryum.Key words: Aphanocladium, Cladobotryum, conidiogenesis, hyphomycetes, molecular phylogeny, phialide, Stanjemonium, systematics, Verticimonosporium.

Spegazzinia camelliae sp. nov. (Didymosphaeriaceae, Pleosprales), a new endophytic fungus from northern Thailand

Phytotaxa ◽  
2021 ◽  
Vol 483 (2) ◽  
pp. 117-128
Author(s):  
NAKARIN SUWANNARACH ◽  
JATURONG KUMLA ◽  
SAISAMORN LUMYONG
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Small Subunit ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Full Description ◽  
Translation Elongation ◽  
Spine Length ◽  
Elongation Factor 1 Alpha

A new endophytic ascomycete, described herein as Spegazzinia camelliae, was isolated from leaves of Camellia sinensis var. assamica collected from Nan Province, Thailand. This species is characterized by basauxic conidiophores and dark brown to blackish brown α and β conidia. It can be distinguished from previously described Spegazzinia species by the spine length of the α conidia and the size of the β conidia. Multi-gene phylogenetic analyses of the small subunit (SSU), large subunit (LSU) and internal transcribed spacers (ITS) of the nuclear ribosomal DNA (rDNA) and the translation elongation factor 1-alpha (tef1) genes also support S. camelliae is a distinct new species within Spegazzinia. A full description, color photographs, illustrations and a phylogenetic tree showing the position of S. camelliae are provided.

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