Electrophoretic analysis of Geoffroea (Leguminosae, Papilionoideae): taxonomic inferences in Argentinean populations

2009 ◽  
Vol 22 (2) ◽  
pp. 137
Author(s):  
Alicia L. Lamarque ◽  
Diana O. Labuckas ◽  
Julián Greppi ◽  
Renée H. Fortunato
Keyword(s):  
Profile Analysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Taxonomic Status ◽  
Electrophoretic Analysis ◽  
Dry Forest ◽  
Protein Patterns ◽  
Phenotypic Differences ◽  
Forest Flora ◽  
Sds Page

The genus Geoffroea Jacq. was circumscribed to two species without varietal division. Nevertheless, in the taxonomic treatment of Argentinean dry-forest flora, on the basis of habit and foliar and floral features, it was possible to distinguish G. decorticans (Hook. & Arn.) Burkart var. decorticans from var. subtropicalis (Lillo) Burkart and to recognise intraspecific variation among the populations of G. spinosa. The purposes of the present study were to provide seed-protein data of Geoffroea species, and to analyse the relationships among them. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) was carried out on mature seeds harvested at different locations. Electrophoretic profile analysis showed outstanding differences between G. decorticans var. decorticans and var. subtropicalis and supported the view that these two taxa are less closely related than previously assumed, warranting their recognition at the varietal level. Moreover, attentive to the differences in the protein patterns from the analysed population of G. spinosa (Argentina: Chaco and Salta provinces), in addition to phenotypic differences, materials from other disjunct areas where this species grows need to be studied to verify their taxonomic status.

Protein Expression in Vibrio parahaemolyticus 690 Subjected to Sublethal Heat and Ethanol Shock Treatments

2008 ◽  
Vol 71 (11) ◽  
pp. 2289-2294 ◽  
Author(s):  
MING-LUN CHIANG ◽  
WEI-LI HO ◽  
ROCH-CHUI YU ◽  
CHENG-CHUN CHOU
Keyword(s):  
Heat Shock ◽  
Vibrio Parahaemolyticus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Test Organism ◽  
Protein Patterns ◽  
One Dimensional ◽  
Sds Page ◽  
Molecular Masses ◽  
Stressed Cells

Cells of Vibrio parahaemolyticus 690 were subjected either to heat shock at 42°C for 45 min or to ethanol shock in the presence of 5% ethanol for 60 min. The protein profiles of the unstressed and stressed V. parahaemolyticus cells were compared. Additionally, the induction of DnaK- and GroEL-like proteins in the unstressed and stressed cells of V. parahaemolyticus was also examined. Analysis with one-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) indicated that three proteins with molecular masses of 93, 77, and 58 kDa were induced by both heat shock and ethanol shock. The protein patterns revealed by two-dimensional electrophoresis were more detailed than those revealed by one-dimensional SDS-PAGE. It was found that heat shock and ethanol shock affected the expression of a total of 28 proteins. Among them, four proteins with molecular masses of 94, 32.1, 26.7, and 25.7 kDa were enhanced by both heat shock and ethanol shock. Furthermore, immunoblot analysis showed the presence of a GroEL-like protein with a molecular mass of 61 kDa in the test organism, with the heat-shocked and ethanol-shocked cells producing a GroEL-like protein in a larger quantity than the unstressed cells. However, DnaK-like protein was not detectable in either the unstressed or the stressed cells.

scholarly journals Comparative studies of Yersinia pestis outer membrane isolation techniques and their potential use in plague epidemiology

1990 ◽  
Vol 32 (2) ◽  
pp. 78-83 ◽  
Author(s):  
Frederico Guilherme Coutinho Abath ◽  
Luís Carlos de Sousa Ferreira
Keyword(s):  
Yersinia Pestis ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Patterns ◽  
Isolation Techniques ◽  
Sds Page ◽  
Triton X ◽  
Potential Use

In the present study three techniques for obtaining outer membrane enriched fractions from Yersinia pestis were evaluated. The techniques analysed were: differential solubilization of the cytoplasmic membrane with Sarkosyl or Triton X-100, and centrifugation in sucrose density gradients. The sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of outer membrane isolated by the different methods resulted in similar protein patterns. The measurement of NADH-dehydrogenase and succinate dehydrogenase (inner membrane enzymes) indicated that the outer membrane preparations obtained by the three methods were pure enough for analytical studies. In addition, preliminary evidences on the potential use of outer membrane proteins for the identification of geographic variants of Y. pestis wild isolates are presented.

scholarly journals Evaluation of numerical analysis of SDS-PAGE of protein patterns for typingEnterobacter cloacae

1989 ◽  
Vol 103 (2) ◽  
pp. 265-274 ◽  
Author(s):  
M. Costas ◽  
L. L. Sloss ◽  
R. J. Owen ◽  
M. A. Gaston
Keyword(s):  
Numerical Analysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Phage Typing ◽  
Clinical Isolates ◽  
Protein Patterns ◽  
One Dimensional ◽  
Sds Page ◽  
Typing Methods ◽  
Type Strains

SUMMARYTwenty cultures comprising 13 clinical isolates ofEnterobacter cloacaefrom two hospitals. the type and another reference stain ofE. cloacaeand the type strains of four otherEnterobactersp. and ofEscherichia coli, were characterized by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into nine clearly defined protein types. Comparison with established typing methods indicated that the discrimination of SDS-PAGE was similar to that achieved with conventional typing methods and all strain groups recognized by combined sero/phage typing were also found by SDS-PAGE. In addition, protein typing sub-divided a group of four serotype O3 isolates that were difficult to distinguish by phage typing. We conclude that high-resolution SDS-PAGE of proteins provides an effective method of typing isolates ofE. cloacae.

scholarly journals Numerical analysis of SDS–PAGE protein patterns ofSerratia marcescens: a comparison with other typing methods

1990 ◽  
Vol 105 (1) ◽  
pp. 107-117 ◽  
Author(s):  
B. Holmes ◽  
M. Costas ◽  
L. L. Sloss
Keyword(s):  
Numerical Analysis ◽  
Reference Strain ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Clinical Isolates ◽  
Protein Patterns ◽  
Sds Page ◽  
Typing Methods ◽  
Effective Adjunct ◽  
Reference Strains

SUMMARYTwenty-five cultures comprising 18 clinical isolates ofSerratia marcescensfrom two hospitals, the type strain ofS. marcescens, two reference strains ofS. marinorubra, the type or a reference strain of three other Serratia species and a reference strain of undetermined species, were characterized by one-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into eight protein types. Comparison with O-serotyping indicated that the level of discrimination by SDS–PAGE was similar. As with O-serotyping, a secondary scheme, such as phage typing, is necessary to differentiate strains of the same protein type. We conclude that high-resolution SDS–PAGE of proteins provides an effective adjunct to other methods for typing isolates ofS. marcescens.

scholarly journals Identification of outbreak-associated and other strains ofClostridium difficileby numerical analysis of SDS-PAGE protein patterns

1994 ◽  
Vol 113 (1) ◽  
pp. 1-12 ◽  
Author(s):  
M. Costas ◽  
B. Holmes ◽  
M. Ganner ◽  
S. L. W. On ◽  
P. N. Hoffman ◽  
...  
Keyword(s):  
Numerical Analysis ◽  
High Resolution ◽  
Clostridium Difficile ◽  
Gel Electrophoresis ◽  
Protein Pattern ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Patterns ◽  
One Dimensional ◽  
Sds Page

SUMMARYSeventy-three cultures ofClostridium difficileisolated both during, and in the period immediately following, an outbreak of infection in a group of three hospitals, were characterized by one-dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) of whole-cell proteins. Each protein pattern was characterized by the presence of one or two dense bands which were highly reproducible. The protein patterns were used as the basis for a numerical analysis which divided the strains into five phenons (electrophoretic or EP types). The majority, 60 of the 73 cultures, belonged to a single phenon which included strains from both patients and the environment. We conclude that high-resolution SDS–PAGE of proteins provides an effective method for typingC. difficileand therefore for tracing the possible spread of epidemic strains in hospitals and other institutions, thereby allowing a better understanding of the epidemiology of the organism.

Echinococcus granulosus in Spain: strain differentiation by SDS-PAGE of somatic and excretory/secretory proteins

1996 ◽  
Vol 70 (3) ◽  
pp. 253-257 ◽  
Author(s):  
M. Siles-Lucas ◽  
C. Cuesta-Bandera
Keyword(s):  
Echinococcus Granulosus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Secretory Proteins ◽  
Protein Patterns ◽  
Experimental Conditions ◽  
Strain Differentiation ◽  
Sds Page ◽  
Natural Hosts ◽  
Host Influence

AbstractA comparison was made, by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), of excretory/secretory (ES) – crude and immunopurified (with the corresponding anti-host serum) hydatid fluids – and somatic (S) – protoscoleces – proteins, from several ovine, equine, swine, bovine and human Echinococcus granulosus Spanish isolates. Likewise, the host influence on parasitic ES protein expression was studied, comparing purified hydatid fluids from ovine and equine cysts obtained from natural hosts and in RNMI mice. Purified hydatid fluids patterns, under reducing conditions, yielded the most precise differentiation of Spanish strains of E. granulosus into three groups (ovine—bovine-human, equine and swine), the finding of a characteristic 82 kDa band in equine isolates, and an unusual arrangement of bands between 50 and 6 kDa in swine samples. In addition, differences were found amongst crude and purified hydatid fluids, especially in bovine and swine isolates. The total protein patterns of protoscoleces were most complex, and therefore could not be used for strain differentiation. Finally, the purified hydatid fluids from cysts developed in natural and experimental hosts showed similar protein patterns, suggesting the lack of host influence, under our experimental conditions, on the expression of parasitic ES proteins.

A simple and rapid method for the differentiation and identification of thermophilic bacteria

2006 ◽  
Vol 52 (8) ◽  
pp. 753-758 ◽  
Author(s):  
Bin Liu ◽  
Hebin Li ◽  
Suijie Wu ◽  
Xiaobo Zhang ◽  
Lianhui Xie
Keyword(s):  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Random Amplified Polymorphic Dna ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Cell Protein ◽  
Protein Patterns ◽  
Simple Method ◽  
Whole Cell ◽  
Sds Page

In total, 170 strains of thermophilic bacteria were isolated from deep-sea hydrothermal fields in the Pacific Ocean and a hot spring in Xiamen of China. To facilitate the identification of thermophilic strains, sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS–PAGE) of whole-cell proteins of these strains was first performed. The results showed that there exist four different protein patterns, indicating that the 170 strains might belong to four species or genera. The RAPD (random amplified polymorphic DNA) profiles of nine representative strains were consistent with those of SDS–PAGE. To further identify the species of the nine strains, their 16S rDNA sequences were analyzed. The results showed that the nine strains fell into four species of three genera, which was the same as revealed by SDS–PAGE. Therefore, SDS–PAGE of whole-cell proteins could be used as a rapid and simple method for the discrimination of thermophilic bacteria as the first step of species identification.Key words: thermophilic bacteria, SDS–PAGE, whole-cell protein, discrimination.

scholarly journals Electrophoretic Identification of Garlic and Garlic Products

1996 ◽  
Vol 79 (6) ◽  
pp. 1466-1470 ◽  
Author(s):  
Emiko Mochizuki ◽  
Takao Yamamoto ◽  
Sumiko Suzuki ◽  
Hiroyuki Nakazawa
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Garlic Extract ◽  
Constant Current ◽  
Protein Patterns ◽  
Simple Method ◽  
Sds Page

Abstract We developed a rapid and simple method for identifying garlic and garlic products using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) with silver staining. Samples were homogenized with 1 % SDS or 6M urea and centrifuged. Supernatant containing garlic proteins was mixed with the same volume of loading buffer containing SDS and mercaptoethanol, heated in boiling water for 2 min, and applied to the wells of a ready-to-use polyacrylamide gradient gel (4–20%). Electrophoresis was performed 20 mA constant current for 2 h. The gel was stained with a silver staining kit and dried. Protein patterns of garlic and garlic products are different from those of other Allium plants such as onion, rakkyo, and caucas. The method was used to analyze samples of spice and garlic clove products. Absence of protein bands in garlic extract products suggests the products may contain less proteins and/or denatured proteins.

scholarly journals Evaluation of Genetic Diversity in Barley (Hordeum vulgare) Genotypes using Protein Profiling

2018 ◽  
Vol 6 (1) ◽  
pp. 23-31
Author(s):  
Manal Eid
Keyword(s):  
Genetic Diversity ◽  
Hordeum Vulgare ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Profiling ◽  
Protein Patterns ◽  
Systematic Classification ◽  
Molecular Weights ◽  
Sds Page ◽  
Solid Foundation

The knowledge of the genetic diversity of barley (Hordeum vulgare) genotypes based on protein polymorphism is very important for breeding programs. The purpose of the current study was to determine the genetic diversity and relationships among ten barley genotypes by using Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) for protein profiles. A total number of 30 bands with molecular weights ranging from 12 to 148 KD were detected. Out of these, five bands were observed monomorphic. Rest of the bands had shown polymorphism to the extent of 83.3% among the test genotypes. The genetic similarity of the ten genotypes tested varied from 0.26 to 1.00 with an average of 0.51. Cluster analysis divided the ten genotypes into two major clusters comprising four subclusters, which was consistent with the systematic classification of barley done in previous studies. The results of this study indicated that the genotypes of barley could effectively be differentiated based on polymorphism, detected between protein patterns. SDS-PAGE presented a higher differentiation power and better repeatability; thus, could be used as a rapid and reliable method for genetic diversity analysis and laid a solid foundation for future barley breeding.

scholarly journals Comparison of SDS–PAGE protein patterns with other typing methods for investigating the epidemiology of ‘Klebsiella aerogenes’

1990 ◽  
Vol 104 (3) ◽  
pp. 455-465 ◽  
Author(s):  
M. Costas ◽  
B. Holmes ◽  
L. L. Sloss
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Phage Typing ◽  
Clinical Isolates ◽  
Klebsiella Aerogenes ◽  
Protein Patterns ◽  
Sds Page ◽  
Typing Methods ◽  
Effective Adjunct ◽  
Type Strains

SUMMARYTwenty–four cultures comprising 20 clinical isolates of ‘Klebsiella aerogenes’ from two hospitals, a reference strain of ‘K. aerogenes’ and the type strains of three otherKlebsiellaspecies, were characterized by one–dimensional sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) of whole–cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into 12 protein types. Comparison with established typing methods indicated that the level of discrimination of SDS–PAGE was similar to that achieved with conventional typing methods but the strains were grouped differently. Protein typing sub–divided five serotype K3 isolates that could also be distinguished by phage typing. Conversely, three strains of protein type 11 were clearly distinguishable by both serotyping and phage typing. We conclude that high–resolution SDS–PAGE of proteins provides an effective adjunct to other methods for typing isolates of ‘K. aerogenes’.

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