A survey of swainsonine content in Swainsona species

2017 ◽  
Vol 39 (2) ◽  
pp. 213 ◽  
Author(s):  
Daniel Cook ◽  
Dale R. Gardner ◽  
Kevin D. Welch ◽  
Jeremy G. Allen
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Lysosomal Storage ◽  
Chromatography Mass Spectrometry ◽  
Chemical Assay ◽  
Large Genus ◽  
Glycoprotein Processing ◽  
Modern Instrumentation

The indolizidine alkaloid swainsonine is an inhibitor of α-mannosidase and mannosidase II that causes lysosomal storage disease and alters glycoprotein processing. Several plant species worldwide contain swainsonine, grazing these plants may cause severe toxicosis in livestock, leading to a chronic disease characterised by altered behaviour, depression, weight loss, decreased libido, infertility and death. Swainsona is a large genus of the Fabaceae family with all species but one being endemic to Australia. Swainsonine has previously been reported to be, or expected to be, present in 26 Swainsona species in Australia. Methods of detection in these 26 species were a jack bean α-mannosidase inhibition assay, gas chromatography, or gas or liquid chromatography coupled with mass spectrometry. Seven of these 26 Swainsona species are reported to be toxic, and for three of these no chemical assay for swainsonine has been undertaken. Only 1 of the 26 species has been analysed for swainsonine using modern instrumentation such as gas or liquid chromatography coupled with mass spectrometry. Using both liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry, 248 specimens representing 41 Swainsona species were screened in the present study for swainsonine. Swainsonine was detected in 9 of the 41 Swainsona species, eight of which had not been determined to contain swainsonine previously using modern instrumentation. The list of swainsonine-containing taxa reported here will serve as a reference for diagnostic purposes and risk assessment.

A Comprehensive Review on Importance and Quantitation of Atypical Antipsychotic Drugs and their Active Metabolites in Commercial Dosage Forms

2020 ◽  
Vol 16 (8) ◽  
pp. 989-1019
Author(s):  
Habibur Rahman ◽  
S.K. Manirul Haque ◽  
Masoom Raza Siddiqui
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Antipsychotic Drugs ◽  
Method Development ◽  
Early Death ◽  
Dosage Forms ◽  
Gas Chromatography Mass Spectrometry ◽  
Active Metabolites ◽  
Chromatography Mass Spectrometry

Background: Schizophrenia is a severe mental illness that affects more than twenty-one million people throughout the world. Schizophrenia also causes early death. Schizophrenia and other related psychotic ailments are controlled by the prescription of antipsychotic drugs, which act by blocking certain chemical receptors in the brain and thus relieves the symptoms of psychotic disorder. These drugs are present in the different dosage forms in the market and provided in a certain amount as per the need of the patients. Objective: Since such medications treat mental disorders, it is very important to have a perfect and accurate dose so that the risk factor is not affected by a higher or lower dose, which is not sufficient for the treatment. For accurate assay of these kinds of drugs, different analytical methods were developed ranging from older spectrophotometric techniques to latest hyphenated methods. Results: The current review highlights the role of different analytical techniques that were employed in the determination and identification of antipsychotic drugs and their metabolites. Techniques such as spectrophotometry, fluorimetry, liquid chromatography, liquid chromatography-mass spectrometry, gas chromatography, and gas chromatography-mass spectrometry employed in the method development of such antipsychotic drugs were reported in the review. Different metabolites, identified using the hyphenated techniques, were also mentioned in the review. The synthesis pathways of few of the metabolites were mentioned. Conclusion: The review summarizes the analyses of different antipsychotic drugs and their metabolites. A brief introduction of illnesses and their symptoms and possible medications were highlighted. Synthesis pathways of the associated metabolites were also mentioned.

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