67 In vivo versus in vitro embryo production after superstimulation of donors in the camel (Camelus dromedarius)

2022 ◽  
Vol 34 (2) ◽  
pp. 269
Author(s):  
B. S. Vettical ◽  
N. A. Wani
Keyword(s):  
Camelus Dromedarius ◽  
Embryo Production ◽  
In Vitro Embryo Production

Predictive value of in vitro embryo production characteristics for in vivo fertility of bulls

1997 ◽  
Vol 47 (1) ◽  
pp. 259 ◽  
Author(s):  
L.M.T.E. Lansbergen ◽  
E.H.A.T. Hanenberg ◽  
A.M. van Wagtendonk-de Leeuw
Keyword(s):  
Predictive Value ◽  
Embryo Production ◽  
In Vitro Embryo Production ◽  
Production Characteristics

scholarly journals 279VASCULAR MORPHOMETRY OF BOVINE PLACENTOMES IN LATE GESTATION FROM EMBRYOS PRODUCED IN VIVO OR IN VITRO

2004 ◽  
Vol 16 (2) ◽  
pp. 259
Author(s):  
J.R. Miles ◽  
C.E. Farin ◽  
K.F. Rodriguez ◽  
J.E. Alexander ◽  
P.W. Farin
Keyword(s):  
Blood Vessels ◽  
Maternal Blood ◽  
Volume Density ◽  
Late Gestation ◽  
Embryo Production ◽  
Vascular Volume ◽  
Treatment Groups ◽  
In Vitro Embryo Production

The role of the vascular supply in the development of placentas from embryos produced in vitro is poorly understood. The objective of this study was to determine the effects of in vitro embryo production on morphometry of blood vessels within fetal (cotyledonary) and maternal (caruncular) components of the placentome during late gestation. In vivo-produced embryos were recovered from superovulated Holstein cows on Day 7 after estrus. For in vitro embryo production, oocytes were aspirated from the ovaries of Holstein cows, matured in vitro, and then fertilized. Presumptive zygotes with their cumulus cells were transferred into M-199 with 10% estrus cow serum and cultured for 168h post-insemination. Semen from the same Holstein sire was used for the production of in vivo and in vitro embryos. Single blastocysts from each production system were transferred into the uteri of heifers. On Day 222 of gestation, fetuses and placentas were recovered in utero (in vivo, n=12; in vitro, n=12). Placentomes were collected, fixed and sectioned. Fetal and maternal blood vessels were identified within placentome sections using immunocytochemistry for vascular endothelial growth factor (VEGF) protein. A total of 4.8×105μm2 of tissue were examined from each placentome. Stereological methods were used to determine the volume densities of fetal and maternal blood vessels. Data were analyzed by GLM procedures. Fetuses were heavier (P=0.03) in the in vitro group (20.7±1.0kg, LS mean±SEM) compared to the in vivo group (17.3±1.0kg). Placentas were also heavier (P=0.06) for the in vitro group (2.5±0.2kg) compared to the in vivo group (2.0±0.2kg). Placental efficiency, calculated as fetal weight/placental weight, was similar between the two treatment groups (9.0±0.5 and 8.9±0.5 for in vivo and in vitro, respectively). Fetal vascular volume density in placentomes was not different between the two treatment groups (5.4±0.3% and 5.4±0.3% for in vivo and in vitro, respectively). In contrast, maternal vascular volume density was greater (P=0.02) for placentomes in the in vitro group (5.9±0.3%) compared to in vivo controls (4.9±0.3%). In summary, compared to placentomes from embryos produced in vivo, placentomes from embryos produced in vitro had similar volume density of fetal vessels, but had significantly increased volume density of maternal vessels. Supported by the State of North Carolina.

97 IN VIVO AND IN VITRO EMBRYO PRODUCTION WITH Y-SEXED SORTED OR CONVENTIONAL SEMEN IN BEEF CATTLE

2014 ◽  
Vol 26 (1) ◽  
pp. 162
Author(s):  
H. Tribulo ◽  
J. Carcedo ◽  
R. Tribulo ◽  
J. Menajovsky ◽  
B. Bernal ◽  
...  
Keyword(s):  
Animal Health ◽  
T Test ◽  
High Fertility ◽  
In Vitro Production ◽  
Embryo Production ◽  
Sexed Semen ◽  
Chi Squared ◽  
In Vitro Embryo Production

An experiment was designed to evaluate in vivo and in vitro embryo production following the use of frozen–thawed conventional or Y-sexed semen from a Brangus bull with known high fertility. For in vivo embryo production, Brangus heifers (n = 12) were superovulated twice in a crossover design and inseminated with sexed or conventional semen. On Day 0, all heifers received an intravaginal progesterone device (DIB 1 g, Syntex S.A., Buenos Aires, Argentina) and 2.5 mg oestradiol benzoate and 50 mg progesterone (Progestar, Syntex S.A.) by intramuscular injection (IM). On Day 4, heifers were superstimulated with 200 mg of NIH-FSH-P1 Folltropin-V (Bioniche Animal Health, Belleville, Ontario, Canada) in twice-daily decreasing doses over 4 days. In the a.m. and p.m. of Day 6, all heifers received PGF2a (Ciclase, Syntex) and DIBs were removed in the p.m.. In the a.m. of Day 8, heifers received 100 μg de Gonadolerin (Gonasyn, Syntex S.A.) and were randomly allocated to receive either one straw of conventional semen (24 × 106 sperm per dose) 12 and 24 h later or two straws of sexed semen (2.4 × 106 sperm per dose) 18 and 24 h after GnRH. Ova/embryos were collected nonsurgically on Day 15 and evaluated following IETS recommendations. Means were compared by t-test. Mean ( ± s.e.m.) number of ova/embryos, fertilized ova, and transferable embryos were 14.8 ± 2.7, 9.4 ± 1.8, and 7.1 ± 1.7 v. 16.8 ± 3.1, 9.9 ± 2.5, and 8.1 ± 2.0 for donors inseminated with conventional or sexed semen, respectively (P > 0.6). For in vitro production, oocytes were obtained from 50 ultrasound-guided follicle aspiration (OPU) sessions that was performed at random stages of the oestrous cycle and without superstimulation in 22 Brangus cows and heifers. Oocytes were classified and matured in TCM-199 medium with NaHCO3 and supplemented with 1% fetal bovine serum. Semen samples from the same bull used for in vivo embryo production were selected using Percoll and capacitated in Fert medium and used at a final concentration of sperm/mL for nonsexed semen and 2 × 106 sperm mL–1 for sexed semen. After 16 h (sexed) or 18 h (conventional) in Fert medium, zygotes were denuded and cultured in SOF supplemented with 0.4% BSA under oil at 37°C, 5% CO2 and saturated humidity for 7 days. The total number of oocytes matured and fertilized was 528 and 318 for conventional and sexed semen, respectively. Means were compared by t-test and proportions by chi-squared test. Mean (± s.e.m.) number of cleaved zygotes and blastocysts produced per OPU session did not differ between conventional (11.0 ± 1.4 and 7.1 ± 1.0) and sexed (8.7 ± 0.8 and 4.9 ± 0.7; P > 0.2) semen. However, the proportion of cleaved zygotes and blastocysts produced were significantly higher (P < 0.05) with conventional semen (61.2%; 329/538 and 39.4%; 212/538) than with sexed semen (54.4%; 173/318 and 30.8%; 98/318), respectively. In conclusion, comparable number of embryos can be obtained in vivo with sexed or conventional semen from a bull with proven high fertility. However, the proportion of blastocysts produced in vitro is likely to be reduced following the use of sexed as compared with conventional semen from the same bull.

In vivo oocyte recovery and in vitro embryo production from bovine donors aspirated at different frequencies or following FSH treatment

1999 ◽  
Vol 51 (5) ◽  
pp. 951-961 ◽  
Author(s):  
K.L Goodhand ◽  
R.G Watt ◽  
M.E Staines ◽  
J.S.M Hutchinson ◽  
P.J Broadbent
Keyword(s):  
Embryo Production ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production

In vitro embryo production compared to in vivo production using gamete recovery and follicular transfer (GRAFT)

1997 ◽  
Vol 47 (1) ◽  
pp. 287
Author(s):  
P. Davies ◽  
J. Singh ◽  
J. Thundathil ◽  
G. Brogliatti ◽  
D. Bergfelt ◽  
...  
Keyword(s):  
Embryo Production ◽  
In Vitro Embryo Production

Effect of season and superstimulatory treatment on in vivo and in vitro embryo production in wood bison ( Bison bison athabascae )

2019 ◽  
Vol 55 (1) ◽  
pp. 54-63
Author(s):  
Jesus Manuel Palomino ◽  
Gabriela F. Mastromonaco ◽  
Miriam P. Cervantes ◽  
Reuben J. Mapletoft ◽  
Muhammad Anzar ◽  
...  
Keyword(s):  
Bison Bison ◽  
Embryo Production ◽  
Wood Bison ◽  
In Vitro Embryo Production
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