117 Nerve growth factor in seminal plasma and its association with extracellular vesicles in South American camelids

2022 ◽  
Vol 34 (2) ◽  
pp. 295
Author(s):  
R. A. Carrasco ◽  
D. J. MacPhee ◽  
G. P. Adams
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Extracellular Vesicles ◽  
Seminal Plasma ◽  
South American ◽  
Nerve Growth ◽  
South American Camelids

scholarly journals β-nerve growth factor identification in male rabbit genital tract and seminal plasma and its role in ovulation induction in rabbit does

2017 ◽  
Vol 17 (2) ◽  
pp. 442-453 ◽  
Author(s):  
Rosa M. Garcia-Garcia ◽  
Maria del Mar Masdeu ◽  
Ana Sanchez Rodriguez ◽  
Pilar Millan ◽  
Maria Arias-Alvarez ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Seminal Plasma ◽  
Genital Tract ◽  
Ovulation Induction ◽  
Nerve Growth ◽  
Male Rabbit

Nerve Growth Factor-β production in the bull: Gene expression, immunolocalization, seminal plasma constitution, and association with sire conception rates

2018 ◽  
Vol 197 ◽  
pp. 335-342 ◽  
Author(s):  
Jamie L. Stewart ◽  
Igor F. Canisso ◽  
Robyn E. Ellerbrock ◽  
Vitor R.G. Mercadante ◽  
Fabio S. Lima
Keyword(s):  
Gene Expression ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Seminal Plasma ◽  
Nerve Growth ◽  
Conception Rates

Nerve Growth Factor-Beta, purified from bull seminal plasma, enhances corpus luteum formation and conceptus development in Bos taurus cows

2018 ◽  
Vol 106 ◽  
pp. 30-38 ◽  
Author(s):  
Jamie L. Stewart ◽  
Vitor R.G. Mercadante ◽  
Nicholas W. Dias ◽  
Igor F. Canisso ◽  
Peter Yau ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Corpus Luteum ◽  
Seminal Plasma ◽  
Bos Taurus ◽  
Nerve Growth ◽  
Conceptus Development ◽  
Growth Factor Beta

scholarly journals Seminal Plasma Induces Ovulation in Llamas in the Absence of a Copulatory Stimulus: Role of Nerve Growth Factor as an Ovulation-Inducing Factor

Endocrinology ◽  
2016 ◽  
Vol 157 (8) ◽  
pp. 3224-3232 ◽  
Author(s):  
Marco A. Berland ◽  
Cesar Ulloa-Leal ◽  
Miguel Barría ◽  
Hollis Wright ◽  
Gregory A. Dissen ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Seminal Plasma ◽  
Neural Signals ◽  
Intact Male ◽  
Nerve Growth ◽  
Group 4 ◽  
Group 3 ◽  
Group 2

Llamas are considered to be reflex ovulators. However, semen from these animals is reported to be rich in ovulation-inducing factor(s), one of which has been identified as nerve growth factor (NGF). These findings suggest that ovulation in llamas may be elicited by chemical signals contained in semen instead of being mediated by neural signals. The present study examines this notion. Llamas displaying a preovulatory follicle were assigned to four groups: group 1 received an intrauterine infusion (IUI) of PBS; group 2 received an IUI of seminal plasma; group 3 was mated to a male whose urethra had been surgically diverted (urethrostomized male); and group 4 was mated to an intact male. Ovulation (detected by ultrasonography) occurred only in llamas mated to an intact male or given an IUI of seminal plasma and was preceded by a surge in plasma LH levels initiated within an hour after coitus or IUI. In both ovulatory groups, circulating β-NGF levels increased within 15 minutes after treatment, reaching values that were greater and more sustained in llamas mated with an intact male. These results demonstrate that llamas can be induced to ovulate by seminal plasma in the absence of copulation and that copulation alone cannot elicit ovulation in the absence of seminal plasma. In addition, our results implicate β-NGF as an important mediator of seminal plasma-induced ovulation in llamas because ovulation does not occur if β-NGF levels do not increase in the bloodstream, a change that occurs promptly after copulation with an intact male or IUI of seminal plasma.

13 EFFECTS OF NERVE GROWTH FACTOR-β, PURIFIED FROM BULL SEMINAL PLASMA, ON CORPUS LUTEUM FUNCTION AND CONCEPTUS DEVELOPMENT IN COWS

2017 ◽  
Vol 29 (1) ◽  
pp. 114
Author(s):  
J. L. Stewart ◽  
V. R. G. Mercadante ◽  
I. F. Canisso ◽  
F. S. Lima
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Corpus Luteum ◽  
Seminal Plasma ◽  
Repeated Measures ◽  
Gradient Elution ◽  
Exchange Chromatography ◽  
Systemic Administration ◽  
Nerve Growth ◽  
Conceptus Development

Nerve growth factor-β (NGF) has been identified in the seminal plasma of livestock and is required for the induction of ovulation in camelids. Until recently, it was thought to play a negligible role in species with spontaneous ovulation. On the contrary, recent studies have shown that systemic administration of NGF, purified from llama seminal plasma, was associated with larger corpus luteum (CL) diameter and higher concentrations of progesterone (P) following ovulation. The objective of the current project was to determine if systemic administration of NGF, purified from bovine seminal plasma, would improve CL formation and enhance embryonic development. Our hypothesis was that systemic administration of NGF at the time of artificial insemination in cows would lead to increased CL volume, increased P secretion, and improved expression of markers of conceptus development and maternal recognition of pregnancy. Seminal plasma was harvested from semen collected by electroejaculation in Angus cross bulls. Purification of NGF was performed using a combination of anion- and cation-exchange chromatography and gradient elution. Beef cows were randomly assigned to CONT (n = 30) or NGF (n = 30) groups and synchronized using a 7-day Co-Synch + CIDR program. At time of insemination (day 0), NGF cows received 296 µg of purified NGF, reconstituted in 12 mL of PBS, and CONT cows received 12 mL of PBS intramuscularly. Blood samples were collected from the coccygeal vein of each cow at days 0, 3, 7, 10, 14, 19, 21, 28, 31, 38, 45, and 66. Ultrasound was performed at each time point for determination of ovarian structures (day 0), corpus luteum volume (all time points), and fetus detection (day 28). Statistical analysis was performed using analysis of variance with repeated-measures in R (R version 3.2.2; https://www.r-project.org/). At day 28, 17/30 (57%) CONT cows and 21/30 (70%) NGF cows were diagnosed as pregnant (P = 0.15). At day 0, cows that later became pregnant had a larger follicular diameter (1.46 cm) than those diagnosed as open (1.16 cm; P < 0.01). Follicular diameter at day 0 did not differ significantly between CONT and NGF groups for either open (P = 0.35) or pregnant (P = 0.90) cows. CL volume in open cows was affected by day (P < 0.001) with no treatment (P = 0.84) or treatment by day (P = 0.42) interaction. CL volume in pregnant cows was affected by both day (P < 0.001) and treatment (P < 0.001), with CONT cows having a higher CL volume than NGF cows. Though NGF appeared to have an inhibitory effect on CL volume in pregnant cows, results are still pending for quantification of P, insulin-like growth factor 1, pregnancy-specific protein B, and interferon-stimulated genes, which will more accurately assess the effects that NGF may have on conceptus development. The results of this study will help us to better understand the role of the seminal plasma protein, NGF, at ovulation and determine if it can be utilised to enhance insemination programs in cattle.

Luteotrophic effect of ovulation-inducing factor/nerve growth factor present in the seminal plasma of llamas

2014 ◽  
Vol 81 (8) ◽  
pp. 1101-1107.e1 ◽  
Author(s):  
C. Ulloa-Leal ◽  
O.A. Bogle ◽  
G.P. Adams ◽  
M.H. Ratto
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Seminal Plasma ◽  
Nerve Growth

Proteomic identification of camel seminal plasma: Purification of β-nerve growth factor

2013 ◽  
Vol 136 (4) ◽  
pp. 289-295 ◽  
Author(s):  
Sanjay Kumar ◽  
Vinay Kumar Sharma ◽  
Sudhuman Singh ◽  
Guru Rao Hariprasad ◽  
Gorakh Mal ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Seminal Plasma ◽  
Nerve Growth ◽  
Proteomic Identification

Monoclonal antibodies to nerve growth factor from bovine seminal plasma

1985 ◽  
Vol 22 (9) ◽  
pp. 1061-1072 ◽  
Author(s):  
John G. Steele ◽  
B.A. Dalton ◽  
H. Hoffman ◽  
P.A. Underwood ◽  
D. Rathjen
Keyword(s):  
Monoclonal Antibodies ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Seminal Plasma ◽  
Nerve Growth
Sign in / Sign up

Export Citation Format

Share Document