116 Differences between 5- and 6-day progestogen-based oestrus induction protocol in Saanen and Toggenburg goats

2021 ◽  
Vol 33 (2) ◽  
pp. 166
Author(s):  
J. B. S. Pinheiro ◽  
J. M. G. Souza-Fabjan ◽  
L. F. L. Correia ◽  
M. E. F. Oliveira ◽  
J. F. Fonseca
Keyword(s):  
Animal Health ◽  
Hormonal Treatment ◽  
Induction Treatment ◽  
Device Removal ◽  
Short Term ◽  
Nonbreeding Season ◽  
Follicular Wave ◽  
Minas Gerais State ◽  
Oestrus Induction ◽  
Intravaginal Device

Hormonal oestrus induction treatment is an important tool for the success of reproductive biotechniques in anoestrus goats. In the nonbreeding season, oestrus induction can be performed by the use of several protocols, including those varying in their duration. This study aimed to compare short-term (for either 5 or 6 days) progestogen-based oestrus synchronization protocols in dairy [Toggenburg (n=12) and Saanen (n=12)] goats during the nonbreeding season. This study was performed in Minas Gerais State, Brazil (21°35′S and 43°15′W), with 12 nulliparous (12–14 months old) and 12 pluriparous (2–4 years old in final third of lactation) goats, all with body condition scores (BCS) of 3.0 to 3.5. They were equally assigned to both treatments according to their order of parturition, BCS, and breed. On a random day (Day 0), 24 goats received an intravaginal device of 60mg of medroxyprogesterone acetate (Progespon®, Zoetis) which remained for 5 (G5; n=12) or 6 (G6; n=12) days. On Day 5, goats from both groups received 30µg of cloprostenol intramuscularly (IM) (Prolise®, ARSA S.R.L.) and 200IU of equine chorionic gonadotrophin (eCG) IM (Novormon®, Schering Plough Animal Health). Both groups received cloprostenol and eCG on the same day (regardless of device removal) to ensure they were administered at the same follicular wave. Both device insertion and removal were performed in the morning. Ultrasound evaluations were performed every 12h from device removal to ovulation detection. Ovulations were considered as the average interval between the last observation of follicles and the first exam in which it was no longer seen. The normal distribution of variables was determined by Shapiro-Wilk test and homocedasticity by Levene’s test. All variables were not normally distributed and thus analysed by Mann–Whitney U test, with P<0.05 considered significantly different. Values are presented in median±interquartile range. There was no difference between G5 and G6, respectively, in the interval to oestrus (48.0±0.0 vs. 48.0±12.0h), interval from oestrus onset to ovulation (18.0±9.0 vs. 18.0±12.0h), interval from device removal to ovulation (66.0±18.0 vs. 66.0±24.0h), number of ovulated follicles (2.0±2.0 vs. 2.5±2.0), or mean diameter of ovulated follicles (6.6±1.6 vs. 6.6±1.4mm). All ovulations were detected in the morning. In conclusion, our data demonstrate that short-term hormonal treatment for 5 or 6 days is similarly efficient to induce synchronous oestrus in Saanen and Toggenburg goats. This research was supported by EMBRAPA (Project 20.19.01.004.00.03.001) and CNPq (314952/2018-7).

17 PREGNANCY RATE OBTAINED WITH THE REUTILIZATION OF INTRAVAGINAL DEVICE DICO AFTER 6 DAYS OF TREATMENT FOR ESTRUS SYNCHRONIZATION IN SHEEP

2011 ◽  
Vol 23 (1) ◽  
pp. 115
Author(s):  
P. C. dos Santos-Neto ◽  
C. García-Pintos ◽  
A. Menchaca
Keyword(s):  
Pregnancy Rate ◽  
Body Condition Score ◽  
Intrauterine Insemination ◽  
Prostaglandin F2α ◽  
Follicular Development ◽  
Device Removal ◽  
Fertility Rates ◽  
Short Term ◽  
Serum Progesterone ◽  
Intravaginal Device

The new intravaginal device DICO (Dispositivo Intravaginal Caprino Ovino, 0.3 mg progesterone, Syntex, Buenos Aires, Argentina) is effective in controlling serum progesterone concentrations, follicular development and time of ovulation in sheep, even in its second use after 6 days of treatment (Vilariño et al. 2010 Small Rumin. Res. 91, 219–224). The pregnancy rate obtained with DICO in its first use is similar to that obtained with CIDR-G (Pfizer, New York, NY, USA) (abstract of García-Pintos et al. in this issue). However, pregnancy rate with the reutilisation of DICO has not been evaluated yet. The aim of this study was to determine fertility rates in ewes synchronized with a reused DICO in comparison with a reused CIDR-G. The experiment was carried out on 314 Corriedale multiparous ewes during breeding season (March, 33°S, Uruguay), with a body condition score of 3.0 ± 0.1 (scale 0–5). Ewes received a short-term-protocol using DICO (n = 139) or CIDR-G (n = 175) during 6 days, previously used for 6 days. One dose of prostaglandin F2α analogue (125 μg cloprostenol, Ciclase DL, Syntex) and 300 IU eCG (Novormon, Syntex) were given at the time of device removal. Cervical insemination (200 million spermatozoa) or laparoscopic intrauterine insemination (100 million spermatozoa) was performed with fresh semen at fixed-time 48 or 54 h from device removal, respectively. Pregnancy diagnosis was done by transrectal ultrasonography (5 MHz, Well D, Beijing, China) 40 days after insemination. Pregnancy rate was analysed by logistic regression, and number of fetus/pregnant ewes and fetus/inseminated ewes was analysed by Poisson regression. No differences were found between reused DICO and CIDR-G devices (Table 1). Overall, pregnancy rate was affected by the method of insemination (P ≤ 0.05), being lower for cervical (37.0%; 57/154) than intrauterine insemination (55.0%; 88/160). In conclusion, the reutilization of DICO devices in the short-term protocol (6 days of treatment) allows similar fertility rates compared to re-used CIDR-G devices, by both cervical and intrauterine insemination. Table 1.Fertility rate obtained with the reutilisation of DICO and CIDR in short-term protocol (6 days of treatment) in sheep

scholarly journals Energy supplementation in Santa Inês sheep subjected to estrus induction treatment

2016 ◽  
Vol 37 (1) ◽  
pp. 145 ◽  
Author(s):  
Sabrina Silva Venturi ◽  
Jeferson Ferreira da Fonseca ◽  
Ana Clara Sarzedas Ribeiro ◽  
Marcos Claudio Pinheiro Rogério ◽  
Juliana De Oliveira ◽  
...  
Keyword(s):  
Induction Treatment ◽  
Dietary Energy ◽  
Reproductive Parameters ◽  
Device Removal ◽  
Short Term ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Santa Inês ◽  
Energy Supplementation

The aim of this study was to evaluate the effect of a 20% increase in dietary energy during short-term estrus induction treatment on the reproductive parameters of Santa Inês ewes. Females (n=43) were allocated into two experimental groups according to the amount of energy inclusion in the diet: maintenance diet or maintenance diet plus 20% energy. Ultrasound examinations were performed in order to detect ovulation. To assess sexual behavior, ewes were teased and further mated. Blood samples were collected for the determination of glucose and insulin concentrations. There was no difference (P>0.05) between groups in the following categories: ovulation rate (80.00% vs. 60.00%), largest follicle diameter (6.00 ± 0.20 vs. 5.90 ± 0.60), interval from device removal to ovulation (52.80 ± 14.87 vs. 59.01 ± 8.34 hours), animals in estrus (75.00% vs. 65.21%), interval from device removal to estrus (30.00 ± 15.49 vs. 30.00 ± 13.35 hours) and conception rate (50.00% vs. 21.73%). There were differences (P<0.05) in the concentrations of insulin and glucose. It can be concluded that the 20% increase in energy in the diet during short-term estrus induction treatment did not affect the reproductive parameters studied.

scholarly journals Development of fixed-time artificial insemination protocols for locally adapted Curraleiro Pé-Duro cows

2016 ◽  
Vol 68 (5) ◽  
pp. 1159-1167 ◽  
Author(s):  
R.D. Tortorella ◽  
M.R. Modesto ◽  
J.P. Neves ◽  
A.F. Ramos
Keyword(s):  
Body Condition Score ◽  
Hormonal Treatment ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Device Removal ◽  
Condition Score ◽  
Equine Chorionic Gonadotropin ◽  
Hot Environments ◽  
To Receive ◽  
Intravaginal Device

ABSTRACT Two experiments were conducted aiming to evaluate the effects of two ovulatory inducers (Exp.1) and equine chorionic gonadotropin (eCG; Exp.2) on follicular and luteal dynamics in a fixed-time AI (FTAI) protocol in locally adapted Curraleiro Pé-Duro cows. In Exp. 1 multiparous cows (n=12) received an intravaginal device containing 1g of progesterone (P4) for 8 days and 2mg of estradiol benzoate (EB) intramuscularly (IM) at device insertion (Day 0). At device removal (Day 8) 0.150mg of Sodium D-Cloprostenol was administered IM and the cows were randomly assigned to receive 1mg of EB (EB8) or 1mg of estradiol cypionate (EC8) IM, or to not receive any ovulatory inducer (Control). All the animals participated in all treatments (crossover). The interval from P4 removal to ovulation was shorter and less variable in the EB8 treatment group (P≤0.05). In Exp. 2 (crossover), multiparous cows (n=12) received the same hormonal treatment as the EB8 group in Exp.1. At device removal (Day 8) cows were randomly assigned to receive 300UI of eCG IM or to not receive eCG (Control). No difference was ascertained on follicular and luteal parameters in Exp. 2 (P>0.05). We concluded that EB can be used as the ovulatory inducer (Exp. 1) in a FTAI protocol in Curraleiro Pé-Duro cows. However, eCG (Exp. 2) was not able to stimulate follicular and luteal development. This result is probably due to the adaptive capacity of Curraleiro Pé-Duro cows that maintained a satisfactory body condition score even in dry and hot environments.

30 OVULATION AND PREGNANCY RATES IN CYCLING NELLORE HEIFERS AFTER FIXED-TIME ARTIFICIAL INSEMINATION PROTOCOLS USING DIFFERENT CONCENTRATIONS OF PROGESTERONE, ASSOCIATED OR NOT WITH eCG

2010 ◽  
Vol 22 (1) ◽  
pp. 173
Author(s):  
M. F. Pegorer ◽  
R. L. Ereno ◽  
R. A. Satrapa ◽  
C. M. Barros
Keyword(s):  
Hormonal Treatment ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Repeated Measurements ◽  
Blood Levels ◽  
Dominant Follicle ◽  
Device Removal ◽  
Follicle Diameter ◽  
Intravaginal Device

The objective of the present study was to evaluate the influence of different P4 concentrations, associated or not with eCG, in the ovulation and pregnancy rates of Nellore heifers submitted to hormonal protocols for FTAI. In experiment (exp.) 1, heifers (n = 15) presynchronized with a hormonal treatment were randomly allocated in 4 groups. At the time of intravaginal device insertion (Day 0) all animals received 2 mg of estradiol benzoate (EB) and a intravaginal progesterone-releasing device containing 0.558 g of P4 (Triu®, Biogenesis Bago, Buenos Aires, Argentina; group 0.5G, n = 4) or 1g of P4 (Cronipres®, Biogenesis Bago; group 1G, n = 4), or 0.558 g of P4 and PGF2α (150 μg of D-cloprostenol, group 0.5G/PGF, n = 4), or 1 g of P4 and PGF2α (group 1G/PGF, n = 3). Eight days later (Day 8), PGF2α (150 μg D-cloprostenol) was administered to all heifers, and the P4 source was removed. Twenty-four hours after removing the intravaginal device (Day 9), the heifers were treated with EB (1 mg, i.m.). Blood samples were collected on Days 0, 1, 2, 4, 8, and 10 to evaluate plasma P4 concentration. In exp. 2, cycling Nellore heifers (n = 292) were treated with the same protocols as in the exp. 1. However, 30 to 36 h later all animals were FTAI on Day 10. In exp. 3, heifers (n = 459) received the treatments described for groups 0.5G/PGF and 1G/PGF associated with 300 IU of eCG (i.m. groups 0.5G/PGF/eCG and 1G/PGF/eCG) at the time of intravaginal device removal (Day 8). In a subset of heifers from each group, ovarian ultrasonography was performed to determine the diameter of the dominant follicle on Day 9 (exp.3) and the ovulation rate on Day 12 (exp. 2 and 3). ANOVA for repeated measurements was used to compare P4 levels, and the binary variables (ovulation and pregnancy rates) were analyzed by logistic regression. In exp. 1, P4 concentrations were higher in animals that received 1.0 compared with 0.558 g of P4, and lower in heifers that received PGF2α on Day 0. In both exp. 2 and 3 there were no significant differences in ovulation (65 to 77%) and pregnancy (exp.2: 26 to 33%; exp.3: 39 to 43%) rates among the several groups. In exp. 3, the animals that received 0.558 g of P4 had a larger diameter of the dominant follicle on Day 9 compared with heifers treated with 1.0 g (10.3 ± 0.2 v. 9.3 ± 0.2 mm; P < 0.01). It is concluded that reduction of P4 concentration in the intravaginal device or the administration of PGF2α at the beginning of the treatments decreases blood levels of P4 between Days 1 and 8 of the FTAI protocols. However, although the use of intravaginal devices with lower P4 contributed to increase the dominant follicle diameter on Day 9, there was no significant increase on ovulation and pregnancy rates. Additionally, the association of eCG with FTAI protocols did not improve follicular growth or ovulation and pregnancy rates. Supported by FAPESP (Sao Paulo, Brazil).

scholarly journals The use of a ‘first-wave’ model to study the effect of nutrition on ovarian follicular dynamics and ovulation rate in the sheep

Reproduction ◽  
2010 ◽  
Vol 140 (6) ◽  
pp. 865-874 ◽  
Author(s):  
C Viñoles ◽  
B Paganoni ◽  
K M M Glover ◽  
J T B Milton ◽  
D Blache ◽  
...  
Keyword(s):  
Body Condition ◽  
Wave Model ◽  
Ovulation Rate ◽  
Short Term ◽  
Metabolic Hormones ◽  
Follicular Waves ◽  
Follicular Wave ◽  
The Third ◽  
High Concentrations ◽  
Follicular Dynamics

We have developed an experimental model in which groups of ewes are simultaneously experiencing the first ovarian follicular wave of their oestrous cycle. We used this ‘first-wave model’ in a 2×2 factorial experiment (ten ewes per group) to study the effect of body condition (BC) and a short-term supplement on follicular dynamics and ovulation rate. The ‘first-wave’ was established by giving ewes three injections of prostaglandin (PG), 7 days apart. The 6-day supplement (lupin grain) began 2 days after the second PG injection and continued until the third. Follicles were studied by ultrasound, and blood was sampled to measure glucose and hormones. The supplement increased (P<0.01) the concentrations of glucose, insulin and leptin, decreased FSH concentrations (P<0.01) and tended to increase oestradiol concentrations (P=0.06). The supplement tended to increase the number of 3 mm follicles (P=0.06). Compared with low-BC ewes, high-BC ewes had more follicular waves (P<0.05), higher concentrations of insulin, leptin and IGF1 (P<0.05) and tended to have higher FSH concentrations (P=0.09). Leptin and insulin concentrations remained high until the end of supplementation in high-BC ewes, whereas they decreased after the third day of supplementation in low-BC ewes. In conclusion, high concentrations of metabolic hormones in fat ewes are associated with the development of more follicular waves. When a supplement is superimposed on this situation, changes in glucose and metabolic hormones allow more follicles to be selected to ovulate.

scholarly journals Use of GnRH for Synchronization of the Follicular Wave in Assisted Reproductive Technologies in Sheep: A Preliminary Study

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1208
Author(s):  
Aina Año-Perello ◽  
Zurisaday Santos-Jimenez ◽  
Teresa Encinas ◽  
Paula Martinez-Ros ◽  
Antonio Gonzalez-Bulnes
Keyword(s):  
Luteinizing Hormone ◽  
Assisted Reproductive Technologies ◽  
Ovarian Response ◽  
Reproductive Technologies ◽  
Estrus Synchronization ◽  
Short Term ◽  
Follicular Wave ◽  
Preliminary Study ◽  
Cost Efficient ◽  
Set Up

The present study aimed to set up a short-term protocol for synchronization of follicular wave emergence in sheep, concomitant with estrus synchronization, which would improve ovarian response in assisted reproductive technologies. Administration of a single GnRH dose, concomitant with the insertion of a progesterone-loaded CIDR device, caused regression of gonadotrophin-dependent follicles ≥4 mm in all the GnRH-treated sheep and in around 80% of the controls treated only with CIDR (p < 0.05). Similar percentages of ewes lost all follicles (around 70%) or only the largest one (around 30%) in both groups. Hence, 54.1% and 70% of the sheep lost all large follicles and initiated a new follicular wave in the control and GnRH groups, respectively (p < 0.05). The remaining sheep showed follicles that were still not dependent of luteinizing hormone (LH). So, in fact, all the sheep had non-dominant follicles after treatment. In conclusion, a treatment including GnRH at CIDR insertion would offer a time- and cost-efficient protocol for inducing follicular turnover and synchronizing a new follicular wave at any stage of the estrous cycle.

scholarly journals Suitability of Different Deterrents for Short-Term Reduction of Wild Boar Contacts With Carcasses of African Swine Fever-Infected Animals

2020 ◽  
Author(s):  
Nicolai Denzin ◽  
Frithjof Helmstaedt ◽  
Carolina Probst ◽  
Franz J. Conraths
Keyword(s):  
Viral Infection ◽  
Disease Control ◽  
Wild Boar ◽  
Animal Health ◽  
African Swine Fever ◽  
Short Term ◽  
Major Threat ◽  
Physical And Chemical ◽  
Important Disease

African swine fever (ASF) is a viral infection of pigs and represents a major threat to animal health and trade. Due to the high tenacity of the causative virus also in carcasses of wild boar, contacts of wild boar with infectious carcasses are regarded an important driver of the so-called habitat cycle. The latter is believed to play a major role in maintaining the present ASF situation in wild boar in Europe. Therefore, search campaigns and timely removal and disposal of carcasses are considered important disease control approaches. If timely disposal is not feasible due to logistic reasons, deterrence of wild boar could be a provisionary option. The performance of seven deterrents (physical and chemical) was tested in a forest near Greifswald, Germany. Carcasses as entities of attraction for wild boar were substituted by luring sites. It could be demonstrated that certain physical (LED-Blinkers, aluminum stripes) and chemical (Wildschwein-Stopp&trade;, Hukinol&trade;) deterrents are capable of reducing significantly the odds of wild boar contacts to one third. It is recommended to carry a choice of the aforementioned, reasonable and easy to apply deterrents, when carcass search campaigns are launched in case of an outbreak of ASF in wild boar.

Efficient follicular wave synchronization using a progesterone-releasing intravaginal device (PRIDΔ) in Camelus dromedarius

2018 ◽  
Vol 118 ◽  
pp. 203-211 ◽  
Author(s):  
Ayman Abdel-Aziz Swelum ◽  
Islam M. Saadeldin ◽  
Abdullah Moumen ◽  
Hani Ba-Awadh ◽  
Abdullah N. Alowaimer
Keyword(s):  
Camelus Dromedarius ◽  
Follicular Wave ◽  
Intravaginal Device

Effect of short-term calf removal at three stages of a follicular wave on fate of a dominant follicle in postpartum beef cows.

2001 ◽  
Vol 79 (10) ◽  
pp. 2688 ◽  
Author(s):  
B E Salfen ◽  
F N Kojima ◽  
J F Bader ◽  
M F Smith ◽  
H A Garverick
Keyword(s):  
Beef Cows ◽  
Dominant Follicle ◽  
Short Term ◽  
Follicular Wave ◽  
Three Stages ◽  
Calf Removal
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