164 Gonadotrophin-releasing hormone injection and colour flow Doppler ultrasound of the preovulatory follicle as a tool to increase pregnancy outcome after timed AI in beef cows

2020 ◽  
Vol 32 (2) ◽  
pp. 209
Author(s):  
L. Pfeifer ◽  
J. Andrade ◽  
E. Moreira ◽  
G. Silva ◽  
V. Souza ◽  
...  
Keyword(s):  
Blood Flow ◽  
Color Doppler ◽  
Prostaglandin F2α ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Doppler Imaging ◽  
Preovulatory Follicle ◽  
Colour Doppler ◽  
Releasing Hormone ◽  
Gonadotrophin Releasing Hormone

The aims of this study were to determine (1) the association between Doppler vascularisation scores (DVS) of the preovulatory follicle (POF) and fertility of beef cows submitted to timed AI (TAI) and (2) whether cows with low DVS benefit from a gonadotrophin-releasing hormone (GnRH) treatment at TAI. Multiparous lactating Nelore cows (Bos indicus; n=69) from a commercial beef farm in the state of Rondônia, Brazil, were enrolled in this study. Cows received 2mg of oestradiol benzoate intramuscularly (Bioestrogen, Biogénesis Bagó) and an intravaginal progesterone-releasing device (1.9g of progesterone; controlled internal drug release, CIDR) to synchronise follicular wave emergence on Day 0. The CIDR device was removed and cows were treated with 150μg of D-cloprostenol intramuscularly (prostaglandin F2α analogue; Croniben), 1mg of oestradiol cypionate intramuscularly, and 300IU of equine chorionic gonadotrophin (Novormon) intramuscularly on Day 8. Cows were then painted with a tail chalk marker to identify those displaying oestrus. All cows were submitted to TAI 48h after CIDR removal. At TAI, occurrence of oestrus was recorded and all cows were examined using transrectal ultrasonography. Blood flow of the POF was evaluated using colour Doppler imaging. Colour Doppler signals present on the follicular wall were subjectively scored using a 1-to-4 scale (1=absence or very low blood flow, and 4=intense blood flow detected on most of the follicular wall surface) adapted from Ginther (2007Ultrasonic Imaging and Animal Reproduction: Color-Doppler Ultrasonography, pages 87-114). Then, cows were divided into three groups according their DVS of the POF: (1) high DVS (DVS ≥3; n=36), (2) low DVS (DVS <3; n=16), and (3) low DVS (DVS <3; n=17) plus a GnRH treatment at TAI. The diameter of the POF was analysed using analysis of variance (PROC GLIMMIX of SAS; SAS Institute Inc.), and the means were compared among groups using Tukey's test. The proportion of cows that displayed oestrus and pregnancy rates was analysed using chi-square test. Cows in the high-DVS group had a larger POF than cows in the low-DVS and low-DVS-GnRH groups (13.2±0.2, 11.7±0.5, and 12.2±0.4, respectively; P<0.05). The proportion of cows that displayed oestrus was greater (P<0.05) in the high-DVS group (72%, 26/36) than in the low-DVS (37.5%, 6/16) or low-DVS-GnRH (53%, 9/17) groups. Finally, greater (P<0.05) pregnancy rates were observed in cows from the high-DVS (47.2%; 17/36) and low-DVS-GnRH (52.9%; 9/17) groups than in cows from the low-DVS group (18.7%; 3/16). The preliminary results from this study demonstrated that diameter of POF is positively associated with DVS. Moreover, cows that presented POF with higher DVS are more likely to become pregnant, and the administration of GnRH to females with low DVS can increase the fertility of beef cows submitted to TAI protocols. This study received funding support from Embrapa (MP1/PC3 project no. 01.03.14.011.00.00) and from CNPq (universal project no. 407307/2016-8).

scholarly journals The effects of gonadotrophin releasing hormone in prostaglandin F2α-based timed insemination programs for beef cattle

2001 ◽  
Vol 81 (3) ◽  
pp. 335-343 ◽  
Author(s):  
J. A. Small ◽  
J. D. Ambrose ◽  
W. P. McCaughey ◽  
D. R. Ward ◽  
W. D. Sutherland ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Key Words ◽  
Prostaglandin F2α ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Progesterone Concentration ◽  
Gender Ratio ◽  
Plasma Progesterone ◽  
Estrous Synchronization ◽  
Gonadotrophin Releasing Hormone

Trials were conducted in the spring (May; n = 324) and fall (October; n = 132) with crossbred continental-type beef cows assigned on the basis of parity and postpartum interval to one of three timed-AI treatments and one of two post-AI treatments. The timed-AI treatments were: (DPG) double (14 d apart) PGF2α (Lutalyse®) and AI (day = 0) 72 h after the second PGF2α (day –3); (OVS) Ovsynch® with the second GnRH (Factrel®) at 48 h and AI at 66 h; and (BRC) the same as OVS except that the second GnRH was given at the time of AI. Half of the cows within each treatment were given GnRH on day 14. Plasma progesterone concentrations were determined for the day of the first injection and on days –3, 0, 14, and 21. Timed-AI pregnancy was diagnosed by ultrasonography at day 42 and confirmed at calving. For DPG, OVS and BRC, PGF2α responder rates were 75.9, 51.4 and 71.3%, respectively, in spring (P < 0.05) and 70.4, 70.4 and 59.1% in fall (P > 0.05), and AI pregnancy rates were 28.7, 44.9 and 44.4% in spring (P< 0.05) and 25.0, 40.9 and 43.2% in fall (P > 0.05). Post-AI GnRH had no significant effect on pregnancy or conception rates or day 21 progesterone. The use of GnRH in the PGF2α based timed-AI program improved pregnancy rates and the BRC treatment was as effective as OVS. Neither postpartum interval nor initial progesterone concentration influenced (P >0.05) the effect of GnRH on AI pregnancy rate, and GnRH had no effect (P > 0.05) on twinning rate or gender ratio. Key words: Beef cows, estrous synchronization, pregnancy, timed-AI, progesterone

Follicle vascularity coordinates corpus luteum blood flow and progesterone production

2017 ◽  
Vol 29 (3) ◽  
pp. 448 ◽  
Author(s):  
S. G. S. de Tarso ◽  
G. D. A. Gastal ◽  
S. T. Bashir ◽  
M. O. Gastal ◽  
G. A. Apgar ◽  
...  
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Doppler Ultrasonography ◽  
Beef Cows ◽  
Regression Analyses ◽  
Preovulatory Follicle ◽  
Colour Doppler ◽  
Plasma Progesterone ◽  
Colour Doppler Ultrasonography ◽  
Progesterone Production

Colour Doppler ultrasonography was used to compare the ability of preovulatory follicle (POF) blood flow and its dimensions to predict the size, blood flow and progesterone production capability of the subsequent corpus luteum (CL). Cows (n = 30) were submitted to a synchronisation protocol. Follicles ≥7 mm were measured and follicular wall blood flow evaluated every 12 h for approximately 3.5 days until ovulation. After ovulation, cows were scanned daily for 8 days and similar parameters were evaluated for the CL. Blood samples were collected and plasma progesterone concentrations quantified. All parameters were positively correlated. Correlation values ranged from 0.26 to 0.74 on data normalised to ovulation and from 0.31 to 0.74 on data normalised to maximum values. Correlations between calculated ratios of both POF and CL in data normalised to ovulation and to maximum values ranged from moderate (0.57) to strong (0.87). Significant (P < 0.0001) linear regression analyses were seen in all comparisons. In conclusion, higher correlations were observed between the dimensions of POF and/or CL and blood flow of both structures, as well as POF and/or CL blood flow with plasma progesterone concentrations of the resultant CL. These findings indicate that follicle vascularity coordinates CL blood flow and progesterone production in synchronised beef cows.

Estrous synchronization and fertility of cross-bred dairy cows using prostaglandin F2α and gonadotropin releasing hormone

2021 ◽  
pp. 171-175
Author(s):  
Abubakr O. Ismail ◽  
◽  
Erneo B. Ochi ◽  
Ambrose S. Jubara ◽  
Bakri Ahmed A/Rahim ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Releasing Hormone ◽  
Treatment Groups ◽  
Estrous Synchronization ◽  
Descriptive Statistical Analysis

A comparative hormonal study of prostaglandin, gonadotropin releasing hormone and their combination was conducted on 30 randomly selected cross-bred dairy cows of SEMEX project for artificial insemination in Hillat Kuku, Khartoum North, Sudan. The study attempts to determine the response of the animals to hormonal induction and synchronization of estrous as well as fertility following fixed time artificial insemination. Three treatment groups of 10 cows each were undertaken. Groups 1, 2 and 3 involved administration of prostaglandin F2α (PGF2α), gonadotropin releasing hormone (GnRH) and a combination of PGF2α + GnRH, respectively. Descriptive statistical analysis was performed. The results revealed that all the three protocols induced and synchronized estrous were almost synchronous in all the treated groups. However, protocol 3 revealed a significantly (p<0.05) better result of 70% pregnancy rate compared to other two protocols that equally provided 50% pregnancy rates. The study resolves that protocol 3 be highly recommended to change the mindset of farmers about the spread of reproductive technology in Sudan. Protocols 1 and 2 provided acceptable pregnancy rates that can be enhanced with the improvement of management.

Abstract 15137: Vector Flow Mapping is a Novel Useful Technique in Assessing Inertia Force of Late Systolic Aortic Flow and Left Ventricular Early Diastolic Function

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Kazuaki Wakami ◽  
Kenta Hachiya ◽  
Syunsuke Murai ◽  
Tsuyoshi Ito ◽  
Hiroshi Fujita ◽  
...  
Keyword(s):  
Blood Flow ◽  
Time Constant ◽  
Color Doppler ◽  
Left Ventricular ◽  
Doppler Imaging ◽  
Inertia Force ◽  
Lv Function ◽  
Flow Mapping ◽  
Vector Flow Mapping ◽  
Late Systole

Background: We previously reported that the inertia force (IF) of blood flowing out of left ventricle (LV) during late-systole produces greater LV elastic recoil force and brings faster LV relaxation. Vector flow mapping (VFM TM , Hitachi-Aloka) enables us to see blood flow velocity vectors that are generated from conventional color Doppler imaging data at any phase of cardiac cycle without angle dependency. Using VFM, kinetic energy (KE) of ejecting blood flow during systole at the LV outflow tract (LVOT) can be obtained. Thus, we investigated whether the KE obtained at the LVOT during late systole (KE-ls) had any relations with the IF and invasively obtained LV function parameters. Method: Study subjects were 33 patients who underwent diagnostic cardiac catheterization and echocardiographic examination on the same day. Color Doppler images were acquired in the apical 3-chamber view. The frame rate ranged was from 40 to 51 frames per minute. Data analyses were performed offline using the commercially available software (DAS-RS1 TM, Hitachi-Aloka). A data sampling area was set at the level just below the aortic valve in the LVOT. The KE-ls was computed as the sum of KE values computed in frame by frame basis during late-systole; late-systole was defined as the latter one-third of ejecting time. LV pressure wave was obtained using a catheter-tipped micromanometer, and then, the first derivative of LV pressure (dP/dt) and a time constant τ of LV pressure decay during isovolumic relaxation were calculated. From LV pressure-dP/dt relationships (phase loop), the IF was determined. Results: A significant positive correlation was observed between the KE-ls and the IF (r=0.79, p<0.0001). The log transformed KE-ls had significant correlations with both peak negative dP/dt (r=0.53, p<0.01) and the time constant τ (r=-0.67, p<0.0001). Conclusion: VFM is a new useful technique to see blood flow in the LV chamber. Noninvasively obtained KE-ls using VFM, which may be a noninvasive surrogate for the IF, has significant correlations with the parameters of LV relaxation.

scholarly journals Efficacy of colour doppler imaging in prediction of placenta accrete

2017 ◽  
Vol 6 (10) ◽  
pp. 4377
Author(s):  
Rachna Agarwal ◽  
Sruthi Bhaskaran ◽  
Esha Gupta ◽  
Dipanvita Dutta ◽  
Anupama Tandon
Keyword(s):  
Placenta Accreta ◽  
Placenta Previa ◽  
Color Doppler ◽  
Color Doppler Imaging ◽  
Final Diagnosis ◽  
Doppler Imaging ◽  
Colour Doppler ◽  
Grey Scale ◽  
Transfusion Requirements ◽  
Caesarean Hysterectomy

Background: In present scenario of increasing cases of previous caesarean section the diagnosis of Placenta accreta preoperatively is of great value to the attending obstetrician. This helps in preparing, counselling the patient and also in assembling a multidisciplinary team for effective peripartum clinical management of these patients to prevent maternal morbidity and mortality.Methods: One hundred patient with persistent placenta previa after 28 weeks gestation were screened by grey scale B mode sonography. In suspicious cases of placenta accreta, further assessment by colour Doppler ultrasound was done. The color doppler imaging (CDI) criteria used were - diffuse intra parenchymal placental lacunar flow, focal intra parenchymal placental lacunar flow, bladder-uterine serosa interphase hypervascularity, prominent sub-placental venous complex and loss of sub-placental vascular signal in areas lacking peripheral sub-placental hypoechoic zone. Patients were prospectively followed up till delivery and the CDI findings were analysed with reference to final diagnosis made during caesarean section.Results: Six of hundred patients exhibited characteristic CDI patterns highly specific for placenta accreta according to the criteria used. In all 6 patients, morbidly adherent placenta was present intraoperatively. The sensitivity and specificity of CDI in the diagnosis of placenta accreta in presentstudy was 100%. Caesarean hysterectomy was required in five patients. Patients with CDI features of lacunar flow had higher incidence of blood loss, transfusion requirements and need for caesarean hysterectomy compared to patients with nonlacunar flow. The remaining 94 patients with placenta previa, not suspicious for placenta accreta on sonography underwent uncomplicated caesarean section.Conclusions: The use of CDI along with conventional grey-scale sonography improves the diagnostic accuracy for prediction of placenta accreta in patients with persistent placenta previa.

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