146 Tributyltin chloride exposure alters ejaculated bull sperm function and embryo development

2020 ◽  
Vol 32 (2) ◽  
pp. 199
Author(s):  
B. W. Daigneault ◽  
K. E. Latham
Keyword(s):  
Embryo Development ◽  
Sperm Motility ◽  
Male Fertility ◽  
The United States ◽  
National Institutes Of Health ◽  
Sperm Function ◽  
Tributyltin Chloride ◽  
Bull Sperm ◽  
Chi Square Analysis ◽  
Motility Parameters

Male exposure to environmental toxicants can disrupt spermatogenesis and impair sperm function. However, the consequences of environmentally relevant levels of toxicants to ejaculated mammalian spermatozoa on sperm function and male fertility are not well studied. Tributyltin chloride (TBT) is an organotin with historical use as an antifouling agent in paints and is a contaminant of soil and groundwater in the United States. Tributyltin chloride is an endocrine disruptor, is detectable in human cord blood, and has negative effects on female reproduction. We hypothesised that TBT could affect sperm function and thereby affect male fertility. To test our hypothesis, we exposed frozen-thawed bull sperm to environmentally relevant doses of TBT (0, 0.1, 1.0, 10, and 100nM) for 90min and then measured sperm motility parameters, fertilisation, and embryo development by IVF. Briefly, frozen-thawed sperm from two bulls were isolated through a 45:90 Percoll gradient, pooled, and then maintained in noncapacitating conditions at 37°C in Tyrode's albumin lactate pyruvate medium devoid of bovine serum albumin and HCO3 − for 90min. Vehicle control (VC) samples consisted of 0.1% MeOH. Sperm motility kinematics were objectively measured after the addition of treatment and every 30min thereafter using computer-aided sperm analysis (IVOS System, Hamilton Thorne). Five replicates were evaluated, and differences in motility kinematics were analysed by analysis of variance using SAS statistical software (SAS Institute Inc.). Sperm treated with 100nM TBT displayed decreased total motility (88 vs. 79%), progressive motility (80 vs. 70%), curvilinear velocity (100 vs. 88 µ/s), and beat-cross frequency (38 vs. 34Hz) over 90min compared with the VC samples (P<0.05). No differences (P>0.05) were detected among any other treatments. Following 90min of exposure to TBT 100nM, sperm were washed twice by centrifugation and re-extended in fertilisation medium. Abattoir-derived bovine oocytes were fertilised with 100nM TBT and VC-exposed sperm. Embryo cleavage and 8- to 16-cell embryos were quantified at 48 and 72h, respectively, in three replicates, and results were assessed using chi-square analysis. Embryos fertilised by TBT-exposed sperm had reduced cleavage to 2-cell (80 vs. 62%) and 8- to 16-cell morulae stages (56 vs. 24%, respectively; P<0.05). In summary, although sperm kinematics were decreased in TBT-exposed sperm, gross motility parameters remained within acceptable ranges for IVF, suggesting that sperm motility alone is not a sufficient measure of sperm function or indicator of male fertility. In conclusion, ejaculated bull sperm exposed to environmentally relevant levels of TBT for 90min had reduced sperm motility parameters, impaired sperm function, and reduced embryo development potential. Research reported in this publication was supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development of the National Institutes of Health under award number T32HD087166. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

225 DOES THE SUPPLEMENTATION OF THE BOAR SEMEN EXTENDER WITH CARNOSINE, L-HISTIDINE, AND TAURINE PRESERVE SPERM FUNCTION DURING STORAGE?

2008 ◽  
Vol 20 (1) ◽  
pp. 192
Author(s):  
C. Matás ◽  
J. C. Gardón ◽  
F. A. Garcia-Vazquez ◽  
S. Pacchini ◽  
M. Ducci
Keyword(s):  
Sperm Motility ◽  
Membrane Lipids ◽  
Semen Analysis ◽  
Antioxidant Systems ◽  
Ros Generation ◽  
Computer Assisted ◽  
Sperm Function ◽  
Taurine Supplementation ◽  
Boar Semen ◽  
Motility Parameters

High levels of reactive oxygen species (ROS: superoxide, hydroxyl, hydrogen peroxide, nitric oxide, peroxynitrile) endanger sperm motility, viability, and function by interaction with membrane lipids, proteins, and nuclear and mitochondrial DNA (Sikka 2004 J. Androl. 25, 5–18). ROS generation has a significant negative effect on the fertilization rate after IVF, and so measurement of ROS levels in semen specimens before IVF may be useful in predicting the IVF outcome (Agarwal et al. 2005 Fertil. Steril. 84, 228–231). Several compounds of the antioxidant systems have been identified in the epididymal environment, spermatozoa, and seminal plasma. The antioxidants carnosine, L-histidine (Ducci et al. 2006 Pol. J. Vet. Sci. 9, 159–163), and taurine (Van der Horst and Grooten 1966 Biochim. Biophys. Acta. 117, 495–497) have been detected in boar semen and added to the extender in freezing procedures in several species. The main objective of this study was to evaluate the effect of carnosine, L-histidine, and taurine supplementation of the extender on boar sperm functionality as measured by sperm motility during computer-assisted semen analysis (CASA) and by IVF ability using mature oocytes, as previously described (Selles et al. 2003 Reprod. Domest. Anim. 38, 66–72). The sperm-rich fraction from mature fertile boars was diluted with isothermal Beltsville thawing solution (BTS) extender. Diluted semen was placed at 15�C and centrifuged at 800g for 10 min. The semen pellet was resuspended with BTS supplemented by 5 mm of carnosine, L-histidine, or taurine or not supplemented (control) to provide 75 � 106 spermatozoa mL–1 and stored at 15�C for 24 h (IVF assay), or 48 or 120 h (for CASA assay). We observed that the motility parameters were affected by storage time and that the addition of taurine increased the motility at 48 h of storage. Alternately, the addition of L-histidine to the extender reduced significantly the motility parameters after 120 h. The results showed that the addition of L-histidine induced a significant (P ≤ 0.01) decrease of the penetration rate (L-histidine 75.8% v. control 89.9%) and the number of sperm per oocyte penetrated (L-histidine 3.1 v. control 4.1). The rate of male pronuclear formation was not affected by the addition of antioxidants to the extender (over 85% in all cases). The addition of carnosine and taurine had no effect on the IVF parameters. In conclusion the antioxidants carnosine, taurine, and L-histidine affect sperm functionality differently, and further studies are necessary to elucidate what changes in sperm function take place during storage and the mechanisms by which these antioxidants exert their effects. This work was supported by Italian-Spanish research project HI2005-0165 and AGL2006-03495.

Supplementation of sperm media with zinc, D-aspartate and co-enzyme Q10 protects bull sperm against exogenous oxidative stress and improves their ability to support embryo development

Zygote ◽  
2017 ◽  
Vol 25 (2) ◽  
pp. 168-175 ◽  
Author(s):  
Vincenza Barbato ◽  
Riccardo Talevi ◽  
Sabrina Braun ◽  
Anna Merolla ◽  
Sam Sudhakaran ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Embryo Development ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Reproductive Technologies ◽  
Developmental Competence ◽  
Sperm Dna Fragmentation ◽  
Bull Sperm

SummaryHigh levels of reactive oxygen species in the semen of infertile patients or spontaneously generated during in vitro sperm handling may impair sperm quality, fertilization and embryo developmental competence. We recently reported that zinc, d-aspartate and co-enzyme Q10, contained in the dietary supplement Genadis® (Merck Serono), have protective effects on human and bull sperm motility, lipid peroxidation and DNA fragmentation in vitro; furthermore, in bovine, treated spermatozoa had an improved ability to support embryo development. However, only a few studies have investigated the protective role of antioxidants during in vitro sperm handling in the presence of an exogenous oxidative stress. Herein, to simulate such conditions in an animal model, we induced exogenous oxidative stress on spermatozoa through the xanthine–xanthine oxidase system and investigated its effects on sperm function and subsequent embryo developmental competence in the presence of zinc, d-Asp and CoQ10 protection. The main results showed that exogenous oxidative stress decreased sperm motility, increased sperm DNA fragmentation, and reduced fertilization and blastocyst rates and quality. Pre-treatment with zinc, d-aspartate and co-enzyme Q10 before exogenous oxidative stress was able to prevent these effects. Supplementation of sperm culture media with zinc, d-aspartate and co-enzyme Q10 could protect sperm from oxidative stress damage during in vitro handling in assisted reproductive technologies.

Centrifugation on Percoll density gradient enhances motility, membrane integrity and in vitro fertilizing ability of frozen–thawed boar sperm

Zygote ◽  
2013 ◽  
Vol 23 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Michiko Noguchi ◽  
Koji Yoshioka ◽  
Hirokazu Hikono ◽  
Gentaro Iwagami ◽  
Chie Suzuki ◽  
...  
Keyword(s):  
Density Gradient ◽  
Embryo Development ◽  
Sperm Motility ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Sperm Head ◽  
Boar Sperm ◽  
Percoll Density Gradient ◽  
Motility Parameters

SummaryThe effects of Percoll density gradient centrifugation on sperm quality, in vitro fertilizability and developmental capacity of frozen–thawed boar sperm were evaluated. Two-step density gradient centrifugation by Percoll enhanced significantly the motility parameters of sperm compared with a simple centrifugation procedure. Percentages of motile sperm and sperm with intact plasma and acrosome membranes after Percoll separation were significantly greater than those after simple centrifugation. The rates of penetration, cleavage and blastocyst formation after in vitro fertilization were significantly improved by Percoll separation compared with simple centrifugation and were influenced positively by the intactness of sperm head membranes, but not any sperm motility parameters. However, insemination with increased concentrations of sperm prepared by Percoll gradient centrifugation did not improve the success of fertilization and embryo development in vitro. Our results indicate that the integrity of sperm head membranes after Percoll separation is important for successful embryo development in vitro, more so than sperm motility.

NIH Funding Ranked “Per Capita”: An Alternative Method for Assessing Research Productivity

2019 ◽  
Vol 42 (1) ◽  
pp. 57-60
Author(s):  
Kristine M. Kulage ◽  
Joshua R. Massei ◽  
Elaine L. Larson
Keyword(s):  
Faculty Member ◽  
Research Productivity ◽  
The United States ◽  
National Institutes Of Health ◽  
Alternative Method ◽  
Schools Of Nursing ◽  
Per Capita ◽  
Nih Funding ◽  
Member School ◽  
Total Average

Ordinal rankings of schools of nursing by research funding in total dollars awarded by the National Institutes of Health (NIH) is a common metric for demonstrating research productivity; however, these data are not based on the number of doctorally prepared faculty eligible to apply for funding. Therefore, we examined an alternative method for measuring research productivity which accounts for size differences in schools: NIH funding ranked “per capita.” We extracted data on total average funding and compared them with average funding secured per faculty member across top-ranked schools of nursing in the United States from 2013 to 2017. When examining data by number of doctorally prepared faculty, 4 of 12 (33%) schools that ranked lower in total average funding ranked higher in average funding per faculty member. School size is an important but neglected factor in current funding rankings; therefore, we encourage schools to use multiple approaches to track their research productivity.

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

scholarly journals Characteristics of Siberian sturgeon and sterlet sperm motility parameters compared using CASA

2012 ◽  
Vol 20 (2) ◽  
Author(s):  
Piotr Sieczyński ◽  
Jan Glogowski ◽  
Beata Cejko ◽  
Cezary Grygoruk
Keyword(s):  
Sperm Motility ◽  
Siberian Sturgeon ◽  
Motility Parameters

HUBUNGAN PENGGUNAAN KOMPUTER DENGAN KEJADIAN NYERI KEPALA TIPE TEGANG PADA PEKERJA

2020 ◽  
Vol 9 (1) ◽  
pp. 27-38
Author(s):  
Suryanti Suryanti ◽  
Andi Ipaljri
Keyword(s):  
Sampling Technique ◽  
The United States ◽  
Chi Square ◽  
Cross Sectional ◽  
Tension Headaches ◽  
Technological Developments ◽  
Tension Type Headaches ◽  
Chi Square Analysis ◽  
Relationship Of ◽  
The Relationship

In the current era of globalization, humans always interact with technological developments. One result of the development of technology is the use of computers. The use of computers that are not under the ergonomic position causes effects on health, one of them is tension-type headaches. In the United States, 1-4% of patients coming to the Emergency Care Unit complain of headache, 90% of them are tension-type headaches. The proportion of migraine and other headache syndromes in the hospital. Harapan Bunda in 2011 and RS. Camatha Sahidya in Batam in Batam was 5% and 0.7% respectively. Whereas the incidence of tension headaches in 5 puskesmas in Batam in 2014 ranged from 0.5% - 1.3%. The proportion of tension headaches at the BIP Clinic in the January-April 2018 period is 1.5%. Tension-type headaches that occur continuously can cause barriers to daily activities. The purpose of this study is to determine the relationship of computer use with the incidence of tension-type headaches in workers. This research uses a descriptive-analytic method with a cross-sectional approach. The sampling technique is purposive sampling. Of 97 respondents who visited the clinical BIP that met the criteria, it was found that computer user respondents experienced tension-type headaches of 18 people (42.9%) and not tension-type headaches of 24 people (57.1%). Whereas the respondents who were not computer users experienced tension-type headaches of 10 people (18.2%), and non-tension-type headaches of 45 people (81.8%). Bivariate chi-square analysis showed that p = 0.008 (p <0.05). This means that Ha is accepted and H0 is rejected.

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