35 EFFECTS OF THAWING TEMPERATURE OF FROZEN SEMEN ON VIABILITY OF REFROZEN AND THAWED CHICKSO (KOREAN BRINDLE CATTLE) AND KOREAN ALBINO CATTLE SPERMATOZOA

2016 ◽  
Vol 28 (2) ◽  
pp. 147
Author(s):  
S. W. Kim ◽  
C. Y. Choe ◽  
D. K. Kim ◽  
A. R. Choi ◽  
H. H. Seong
Keyword(s):  
Genetic Resources ◽  
Sperm Concentration ◽  
Cooling Rates ◽  
Mean Values ◽  
Freeze Thaw ◽  
Frozen Semen ◽  
Native Cattle ◽  
Viable Sperm ◽  
The Mean ◽  
Thawing Procedure

Germplasm cryopreservation from a desired species with agricultural and genetic importance would protect them from the risk for extinction. Semen freezing from Korean native cattle would be a good approach for protecting genetic resources due to their limited numbers. It has been known that sperm could resist cryo-damages by freeze-thaw cycles. Thus, we performed 2 refreezing experiments with different initial thawing temperatures using frozen Korean native cattle semen. A total of 5 Hanwoo, Korean Albino, and brindle cattle were used as semen donors. After thawing by using 5°C/2 min or 37°C/40 s with cooling rates, the semen was diluted with the same volume of cryo-media in the first thawing temperature and refrozen. Sperm motilities were determined and compared between animals and groups after rethawing. The mean sperm concentration and motility was 45 × 106 mL–1 (range 2.3 to 89 × 106 mL–1) and 40% (range 13 to 55%). Mean values of motility and viability of sperm that underwent second preservation were significantly higher in 5°C than in 37°C (P < 0.01). However, the activity of viable sperm thawed at 5°C was significantly decreased before refreezing. It is estimated that refreezing of frozen semen from rare Korean native cattle is possible with resistant properties of survived spermatozoa. The higher motility and viability of refrozen semen could be obtained with 5°C thawing procedure for reuse of frozen semen.

scholarly journals Changes in selected physicochemical parameters of breast muscles of geese from Polish conservation flocks depending on duration of the post slaughter period

2008 ◽  
Vol 51 (3) ◽  
pp. 255-265 ◽  
Author(s):  
A. Okruszek ◽  
J. Książkiewicz ◽  
J. Wołoszyn ◽  
G. Haraf ◽  
A. Orkusz ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Physicochemical Parameters ◽  
Ph Value ◽  
The Other ◽  
Mean Values ◽  
Qualitative Traits ◽  
Ph Values ◽  
Colour Parameters ◽  
The Mean

Abstract. In four Polish conservative flocks of Suwałki (Su), Kartuzy (Ka), Kielce (Ki) and Subcarpathian (Sb) geese, registered by FAO as domestic genetic resources the mean values of qualitative traits of breast muscles (pH15', pH30', pH45', pH24h; colour parameters: L*15', L*30', L*45', L24h, a*15', a*30', a*45', a*24h, b*15', b*30', b*45', b*24h and conductivity: EC45', EC3h, EC24h) were determined. The experimental flocks showed significant variations in the investigated parameters. The Kartuzy goose breast muscles were characterized by higher values of pH15' (6.74), pH30' (6.62), pH45' (6.56) and b*15' parameter (0.80) in comparison with the remaining flocks. Moreover, these muscles had the lowest pH24h (5.65) and values of L* parameter (L*15', L*30', L*45' and L*24h) and EC45' (3.23 mS/cm). The pH value and L* and a* parameters (except L*24h and a*24h) of the analysed flocks were decreasing, and conductivity increasing together with the time post slaughter, and the rate of changes depended on the birds’ origin. Greater differences between pH values, measured 15 min and 24 h after slaughter were stated in breast muscles from Ka (1.09) and Su (0.90) geese than from the other two flocks investigated (Ki – 0.74; Sb – 0.58). The highest increase in conductivity (from EC45' to EC24h) was affirmed in Ka and Ki muscles (3.84 mS/cm) and the lowest in breast muscles of Sb geese (2.72 mS/cm).

scholarly journals Reproductive biology of Pêga Jackasses: characteristics of sperm-rich fraction collected using the split-ejaculate method

2016 ◽  
Vol 68 (4) ◽  
pp. 832-838
Author(s):  
L.E. Carvalho ◽  
J.M. Silva Filho ◽  
M.S. Palhares ◽  
A.L.R. Sales ◽  
A.T. Gonczarowska ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
Morphological Characteristics ◽  
High Quality ◽  
Open Model ◽  
Mean Values ◽  
Normal Sperm ◽  
Semen Volume ◽  
The Mean ◽  
Semen Processing ◽  
Artificial Vagina

ABSTRACT The aim of this study was to evaluate the physical and morphological characteristics of the sperm-rich fraction of jackass semen. To this end, 130 ejaculates from five Pêga jackasses were collected using an open model artificial vagina. The sperm-rich fraction was collected using the split-ejaculate method and assessed for the number of mounts/ejaculate, for physical and morphological characteristics of the semen and number of doses produced/ejaculate. It was observed that all characteristics evaluated differed among the five jackasses, except for the head defect rates. The mean values obtained for the jackass sperm-rich fraction collected were: number of mounts/ejaculate - 1.27; semen volume - 20.21mL; motility - 84.53%; vigor - 4.46; motility after dilution - 80.10%; sperm concentration/mL - 894.38 x 106; total sperm/ejaculate - 16.14 x 109; number of insemination doses/ejaculate (400x106 motile sptz) - 33.39; number of insemination doses/ejaculate (800 x 106 motile sptz) - 16.69; and percentage of normal sperm - 90.46%. Thus, in the present experiment the split-ejaculate method using an open artificial vagina worked well with the jackasses, and the sperm-rich fraction of the ejaculate of Pêga jackasses had high quality and sperm concentration, allowing its use for semen processing without reducing the number of insemination doses produced per ejaculate.

scholarly journals Effect of preservation time on the quality of frozen semen in indigenous rams

2015 ◽  
Vol 44 (1) ◽  
pp. 10-15 ◽  
Author(s):  
BBA Mahmuda ◽  
Azizun Nesa ◽  
BF Zohara ◽  
MGS Alam ◽  
FY Bari
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Volume Density ◽  
Normal Morphology ◽  
Mean Values ◽  
Frozen Semen ◽  
Significant Difference ◽  
The Mean ◽  
Fresh Semen

The study was carried out to observe the effects of preservation time on the quality of frozen semen of indigenous rams. Semen was collected using AV once a week from 4 rams. Tris based with 10% egg yolk and 7% glycerol extender was used to extend and freezing the semen. Fresh semen was evaluated for volume, density, mass motility and concentration, and mean values were observed as 0.8±0.2ml, 3.0±0.3, 3.2±0.7, 3.9±0.7×109/ml, respectively. Significant difference (p<0.05) was found for all the parameters among the rams. Mean values of motility, viability and normal morphology percentages were 83.3±4.3%, 88.2±4.4%, 84.2±3.5% in fresh semen while those of chilled semen at 40C were 74.7±2.3, 78.8±4.9 and 79.2±2.9%, respectively. For all the parameters, significant (p<0.05) difference was found among the rams. Frozen sperm motility was observed after thawing at 39-400C for 14-15 seconds. The mean motility, viability and normal morphology percentages after freezing for 24hrs, 7, 15 and 30 days of duration were 39.8±3.1, 41.1±4.3, 40.1±4.1 and 39.4±2.9%; 44.5±2.5, 45.3±2.8, 44.6±2.8 and 43.9±2.8%; 71.0±2.0, 71.7±1.5, 70.7±1.7 and 70.3±1.8%, respectively and values did not decrease significantly (p>0.05) with the increasing time of preservation. Non significantly decrease of the semen quality with advance of preservation time indicates the suitability of the protocol used for freezing of indigenous ram semen in Bangladesh.DOI: http://dx.doi.org/10.3329/bjas.v44i1.23113            Bang. J. Anim. Sci. 2014. 44 (1): 10-15

46 Effect of Bioxcell® and Triladyl® Extenders on Washed Semen of South African Indigenous Bucks

2018 ◽  
Vol 30 (1) ◽  
pp. 162
Author(s):  
L. P. Nethenzheni ◽  
M. L. Mphaphathi ◽  
N. C. Negota ◽  
T. L. Nedambale
Keyword(s):  
Treatment Group ◽  
South African ◽  
Liquid Nitrogen ◽  
Seminal Plasma ◽  
Semen Parameters ◽  
Mean Values ◽  
Treatment Groups ◽  
Frozen Semen ◽  
The Mean

Semen extenders and seminal plasma are vital for cryopreservation of buck semen. The objectives of the study were to evaluate the effect of 2 extenders: Triladyl® (Minitube, Tiefenbach, Bavaria) and Bioxcell® (IMV, L’Aigle, France) and the removal of seminal plasma on buck semen. Six indigenous bucks were used in this study and 6 ejaculates were collected from individual bucks. The semen was pooled and then randomly allocated into 6 groups: (1) raw-washed, (2) raw-non-washed, (3) Triladyl®-washed, 4) Triladyl®-non-washed, (5) Bioxcell®-washed, and (6) Bioxcell®-non-washed. Spermatozoa viability was assessed using Eosin-Nigrosin and morphology using Spermac® (Vitrolife, Göteborg, Sweden) stains. The washed semen samples were all diluted into (1:4 v/v) with PBS and centrifuged at 1500 × g for 10 min. Semen samples were then extended with Triladyl® or Bioxcell® per treatment groups and equilibrated for 2 h at 5°C. The semen samples were loaded into straws per treatment groups and placed 5 cm above a liquid nitrogen vapour for 10 min and then stored at –196°C until use. After 1 month of storage, frozen semen straws per treatment group were thawed at 37°C for 30 s, and spermatozoa parameters were analysed post-thaw. Significant differences among the mean values of semen parameters were determined by Tukey’s test using ANOVA, GLM procedure of SAS version 12.1 of 2010 (SAS Institute Inc., Cary, NC, USA). There was a higher (P < 0.05) live and normal spermatozoa percentage in non-washed semen extended with Bioxcell® (45.7 ± 21.2) than the semen extended with Triladyl® (24.5 ± 22.2%). Live and normal spermatozoa percentages were drastically reduced in the Bioxcell® (5.2 ± 4.9) and Triladyl® (6.9 ± 8.6%) washed semen groups. There was a higher (P < 0.05) percentage of spermatozoa with head abnormalities in non-washed semen extended with Triladyl® (20.4 ± 10.2), compared with the semen extended with Bioxcell® (18.3 ± 12.4%) following freeze-thawing. There was a higher (P < 0.05) percentage of spermatozoa with head abnormalities in washed semen samples extended with Triladyl® (34.0 ± 16.0) compared with the semen extended with Bioxcell® (10.1 ± 7.0%). There were higher (P < 0.05) percentages of spermatozoa with coiled tail abnormalities in washed semen extended with Bioxcell® (65.4 ± 25.0) compared with Triladyl® (35.9 ± 21.6%). In conclusion, the liveability of spermatozoa was negatively affected by washing of semen extended with Bioxcell® and Triladyl® extender. Bioxcell® significantly increased tail abnormalities and Triladyl® gave less protection against head abnormalities following cryopreservation of South African unimproved indigenous bucks’ semen.

scholarly journals Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

2018 ◽  
Vol 4 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Md Abdullah Al Mansur ◽  
Md Golam Shahi Alam ◽  
Pankaj Kumar Jha ◽  
Md Asaduzzaman Rimon ◽  
Nazmun Naher ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Indigenous Sheep ◽  
Field Level ◽  
Frozen Semen ◽  
Significant Difference ◽  
Conception Rates ◽  
Viability Percentage ◽  
The Mean

This study was undertaken to study the AI conception rate using frozen semen at field level. Five farms in Mymensingh, Bangladesh were selected for AI Trial in field ewes. Four rams were selected for semen collection, evaluation, and frozen semen production and further to study conception rate followed by intra-cervical AI in both natural and synchronized ewes. Conception rate were confirmed by non-returned rate and ultrasound scanning at 30-40 days of post insemination. The volume, colour, mass activity, sperm motility, viability, concentration, HOST +ve (%) and normal spermatozoa percentages were 0.8±0.3 ml, 3.9±0.3, 4.4±0.6, 81.3±5.0%, 90.0±4.0%, 3519.0±545.6x106/ml, 87.4±3.3% and 85.6±1.8%, respectively. The sperm concentration of ram R#6 was significantly higher (P<0.05) (4120.5±93.5x106/ml) compared with other rams. The mean motility and viability of pre-dilution, 120 minutes of addition of Part-A, 240 minutes of addition of Part-B and post-thaw were (83.8±4.8%, 81.3±2.5%, 80.0±4.1% and 41.3±9.5%) and (93.3±1.0%, 90.0±1.4%, 88.8±1.0% and 58.3±8.7%), respectively. There were no significant difference (P˃0.05) between pre-dilution and post-dilution sperm motility and viability percentage however, post-thaw sperm motility and viability significantly (P<0.05) decreased compare with the motility and viability of pre-dilution and post-dilution values. Motility and viability percentages of frozen semen did not decrease significantly (P> 0.05) with the increase of preservation time. The mean motility and viability at 24 hrs, day 7, day 15 and day 30 were 41.3±9.5%, 41.5±8.5%, 41.8±9.9% and 40.5±10.2%; and 59.0±10.1%, 58.5±7.7%, 59.0±8.8% and 57.8±8.3%, respectively. The conception rates in natural and synchronized estrous were 26.7% and 25%, respectively. There was no significant difference in conception rates between the natural and synchronized oestrous in field level. However, the present non-return rate and conception rate indicate the suitability of produced frozen semen application in the field level.Asian J. Med. Biol. Res. March 2018, 4(1): 55-62

scholarly journals The effect of freezing on cryosurvival of yak sperm

2018 ◽  
Vol 15 (2) ◽  
pp. 215-218
Author(s):  
S Deori
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Frozen Semen ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
Live Sperm

A study was carried out to study the effect of freezing on cryosurvival of yak semen. Artificial insemination in yak is still in infancy. Semen cryopreservation and use of artificial insemination can be applied in yak husbandry for conservation and rapid multiplication of superior germplasm. Semen was collected from four adult yak bulls using artificial vagina method managed under uniform conditions. A total of 40 ejaculates comprising of 10 ejaculates each bull were collected following twice a week schedule and evaluated for fresh semen characteristics. The fresh yak semen characteristics viz. ejaculate volume (ml), mass activity (0-4), initial sperm motility (%), sperm concentration (x 106/ml), live sperm (%), sperm abnormality (%) and intact acrosome (%) were 3.10 ± 0.18, 3.53 ± 0.96, 83.89 ± 2.87, 1180.22 ± 42.32, 77.63 ± 4.23, 8.45 ± 3.33 and 93.61 ± 3.78 respectively. The ejaculates were diluted (1:10) with Tris extender consisting of 6.4 ml glycerol and 20 ml of fresh egg yolk. Straws were equilibrated at 5°C for 4 hours followed by exposure to liquid nitrogen vapour for 10 minutes and finally transferred to liquid nitrogen container for storage. The cryosurvival rate was studied after 7 days of storage in liquid nitrogen. The frozen semen was thawed in warm water (37°C) for 30 seconds for evaluation. Mean values of postthaw sperm motility (%), live sperm (%) and intact acrosome (%) in yaks were 55.67 ± 4.67, 65.62 ± 3.23 and 89.26 ± 3.67 respectively. In conclusion, yak semen has a better cryosurvival while freezing in tris extender with 6.4 per cent glycerol and 20 per cent egg yolk following an equilibration period of 4h.SAARC J. Agri., 15(2): 215-218 (2017)

Fresh and Post-Thaw Seminal Attributes of Jafarabadi Buffalo Bulls

2020 ◽  
Vol 15 (03) ◽  
pp. 45-48
Author(s):  
K. H. Parmar ◽  
FS Kavani ◽  
TK Patbandha ◽  
SS Parikh
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Mean Values ◽  
Abnormal Sperm ◽  
The Mean ◽  
Live Sperm ◽  
One Year ◽  
Acrosomal Integrity

The study was conducted on four Jafarabadi breeding bulls, 5-6 years old to know the fresh and post-thawed seminal characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. The mean values of fresh neat seminal characteristics of Jafarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOS reactive sperm (%) and acrosomal integrity (%) were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43 and 93.56±0.56, respectively, whereas the mean post-thawed sperm characteristics, viz., progressive sperm motility, live sperm (%), abnormal sperm (%), HOS reactive sperm (%), acrosomal integrity (%) and first insemination conception rate (%) observed were 57.60±0.36, 66.34±0.53, 8.85±0.33, 56.97±0.46, 75.26±0.17 and 44.63±0.14, respectively. The semen quality of fresh and post-thawed samples observed was within normal limit for use in breeding program with satisfactory first insemination conception rate.

scholarly journals Comparative Study of Gir Cattle and Surti Buffalo Bulls Semen under Middle Gujarat Climate

2017 ◽  
Vol 13 (01) ◽  
Author(s):  
P. J. Chaudhary ◽  
A. J. Dhami ◽  
D. V. Chaudhari ◽  
K. K. Hadiya ◽  
J. A. Patel
Keyword(s):  
Sperm Concentration ◽  
Membrane Integrity ◽  
Mean Values ◽  
Abnormal Sperm ◽  
Buffalo Semen ◽  
The Mean ◽  
Mass Activity ◽  
Live Sperm ◽  
Surti Buffalo ◽  
Fresh Semen

This study was undertaken during the favourable breeding season of the year 2016-17 on healthy mature Gir cattle and Surti buffalo bulls, three each, at Sperm Station of the College. The ejaculates (9/bull, total 54) collected in the morning using artificial vagina were evaluated for routine seminal attributes, including acrosomal and plasma membrane integrity. The mean values of ejaculate volume, sperm concentration, mass activity (0-5 score), individual sperm motility, live sperm, abnormal sperm, intact acrosome and HOST reactive sperms observed in fresh semen of Gir cattle and Surti buffalo bulls were 6.20±0.42 and 3.34±0.23 ml (P less than 0.01), 1169.44±61.71 and 846.30±54.82 million (P less than 0.01), 3.44±0.09 and 3.42±0.08, 76.53±0.53 and 80.76±0.39 % (P less than 0.05), 81.00±1.32 and 84.73±0.78 % (P less than 0.05), 6.00±0.37 and 5.81±0.40 %, 95.59±0.35 and 95.54±0.25 % as well as 80.30±1.90 and 84.58±0.88 % (P less than 0.05), respectively. The variation between cattle and buffalo semen was significant for most of these traits. The variations between bulls within breed were however not significant. Significant correlations were observed only between mass activity and initial motility (0.62), and live and abnormal sperm (-0.41) in Gir bulls, and for ejaculate volume with sperm concentration (-0.56) and abnormal sperm (0.45), and between live and HOS reactive sperms (0.48) in Surti bulls.

scholarly journals Investigation On Seminal Attributes And Fertility Of Black Bengal Goat

1970 ◽  
Vol 37 (2) ◽  
pp. 17-24 ◽  
Author(s):  
AS Apu ◽  
SS Husain ◽  
MAMY Khandoker ◽  
AHMS Rahman ◽  
DR Notter
Keyword(s):  
Sperm Concentration ◽  
Fertility Rate ◽  
The Other ◽  
Mean Values ◽  
Other Hand ◽  
Significant Difference ◽  
The Mean ◽  
Black Bengal Goat ◽  
Fresh Semen ◽  
Selection Of

A total of 120 ejaculates from six adult Black Bengal bucks were studied to determine the semen attributes and evaluate their fertility. The various semen attributes of fresh semen were volume, sperm concentration, mass motility, live spermatozoa and normal spermatozoa percentage. All the semen attributes differed significantly (P<0.05) except normal spermatozoa percentage between the bucks. A total of 129 goats were inseminated with diluted semen to assess the fertility rate of Black Bengal buck. The mean values for the various semen attributes were: volume 0.58 ± 0.03 ml; sperm concentration 2797.22 ± 18.73 × 106/ml; mass motility 77.82 ± 0.61%; live spermatozoa 86.72 ± 0.54% and normal spermatozoa 91.39 ± 0.24%, respectively in fresh semen. With regard to the diluted semen, individual bucks showed significant difference (P<0.05) on motility, live and normal spermatozoa percentages. The diluted semen motility varied from 68.64 ± 1.66 to 74.55 ± 1.06%. On the other hand, the live and normal spermatozoa percentages of the diluted semen varied from 83.73 ± 0.94 to 89.27 ± 1.40% and 88.73 ± 0.54 to 91.91 ± 0.78%, respectively. From the insemination trial, the average kidding rate obtained was 58.9% in Black Bengal goat. It is suggested that selection of good quality buck semen could improve the overall fertility of Black Bengal goat. DOI: http://dx.doi.org/10.3329/bjas.v37i2.9877 BJAS 2008; 37(2): 17-24

QUALITY TRAITS OF FRESH, REFRIGERATED AND CRYOPRESERVED BUFFALO BULL SEMEN AND THEIR INTERRELATIONSHIPS

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
A.J. Dhami ◽  
D.V. Chaudhari ◽  
Orin Varghese
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Mean Values ◽  
Bull Semen ◽  
Artificial Breeding ◽  
The Mean ◽  
Live Sperm ◽  
C 30

A study was carried out on semen ejaculates (40) of five healthy Surti buffalo bulls during favourable breeding season. The ejaculates with >70% IM were diluted @ 100 million sperms/ml in Tris-fructoseegg yolk-glycerol (TFYG) diluent supplemented with L-cysteine @ 1 mg/ml, and examined for quality parameters such as sperm progressive motility, viability, morphology, acrosomal and plasma membrane integrity. A part of the extended semen was preserved at 5°C and another was processed for ultra-low temperature (-196°C) preservation in LN2 using biofreezer after filled in French mini straws. The mean values of ejaculate volume, sperm concentration, mass activity (0-5 score), individual sperm motility, live sperm, abnormal sperm, intact acrosome and HOS reactive sperms observed in fresh semen were 3.04±0.11 ml, 963.05±50.97 million/ml, 2.97±0.06, 75.00±0.69 %, 88.22 ±0.48 %, 4.40±0.26 %, 93.67±0.31 % and 89.17±0.57 %, respectively. Just after dilution the percentages of progressively motile, live & abnormal sperms, intact acrosomes and HOS reactive sperms were 80.51±0.65, 85.95±0.65, 4.92±2.20, 91.00±2.30, 85.72±0.67, respectively. The corresponding values after 48 hrs of refrigeration (5°C) were 61.75±0.85, 67.85±0.85, 6.45±0.27, 89.05±0.32, 67.30±0.96%, the values at pre-freeze stage (after equilibration) were 72.62±0.69, 78.97±0.93, 5.45±0.25, 90.90±0.35, 78.12±0.79% and at post-thaw stage (37°C/30 sec) 46.50±0.72, 52.97±0.79, 7.10±0.26, 85.73±0.18, 51.62 ±0.82%, respectively. The mean motile spermatozoa observed after 1, 2 and 3 h of post-thaw incubation at 37°C in water bath were 32.75±0.82, 18.88 ±0.70 and 8.88±0.66% (P<0.01), respectively. The semen quality parameters, fresh and cryopreserved were acceptable for artificial breeding use. The seminal traits in initial, 48 hrs refrigerated, pre-freeze and post-thawed samples revealed significant (p<0.01) interrelationships (r = 0.44 to 0.84) between progressive motile sperm, live sperm and HOST reactive sperm directing more emphasis on these quality parameters for better semen evaluation.

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