255 SEMINAL PLASMA PROTEINS: FUNCTIONAL ATTRIBUTES AND POTENTIAL MARKERS OF FERTILITY

2016 ◽  
Vol 28 (2) ◽  
pp. 259
Author(s):  
A. A. Moura
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Seminal Fluid ◽  
Technological Development ◽  
Shotgun Proteomics ◽  
Reproductive Technologies ◽  
Early Embryo ◽  
Domestic Species ◽  
Functional Attributes ◽  
Seminal Plasma Proteins

Mammalian seminal plasma is a complex milieu containing, mostly, secretions of the epididymides and accessory sex glands. After ejaculation, proteins of the seminal fluid interact with sperm and participate in several events, including sperm motility, protection against immune reactions, oxidative processes and microorganisms, interaction with the oviduct epithelium, capacitation, acrosome reaction, and fertilization. Given these aspects, the present talk is focused on seminal plasma proteins and cross-species comparisons of their functional attributes. Experiments that will be presented used gel-based and shotgun proteomics. Binder of sperm proteins are major components of ruminant seminal plasma, while spermadhesins are predominant in the boar and deer (Mazama gouazoubira; Mazama nemorivag) seminal fluid. Annexins (rabbits), clusterin (peccaries: Pecari tajacu), and arginine esterase (coatis: Nasua nasua; dogs) are the most abundant proteins in seminal fluid of other species. Such diversity may reflect differences in how paternal factors modulate fertility. Studies have also reported empirical associations between seminal proteins, sperm freezability, and fertility indexes in ruminants and other domestic species. As evidenced by experiments using IVF, mainly in the bovine, specific seminal proteins influence the fertilizing capacity of both epididymal and ejaculated sperm, as well as early embryo development. In summary, fundamental knowledge gathered by studies of seminal plasma proteome can be used to identify molecular markers of fertility. Availability of such markers will potentially improve the outcome of artificial reproductive technologies. Diversity of seminal plasma composition, however, represents challenges for discovery of fertility markers in unique species. Seminal plasma proteins may also be related to new phenotypes, such as bull differences in sperm survival after sexation. This research was funded in part by the Brazilian Research Councils (CAPES and CNPq) and Ceara State Foundation for Scientific and Technological Development (FUNCAP).

scholarly journals Ram seminal plasma and its functional proteomic assessment

Reproduction ◽  
2019 ◽  
Vol 157 (6) ◽  
pp. R243-R256 ◽  
Author(s):  
T Leahy ◽  
J P Rickard ◽  
N C Bernecic ◽  
X Druart ◽  
S P de Graaf
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Reproductive Tract ◽  
Reproductive Technologies ◽  
Female Reproductive Tract ◽  
Sperm Function ◽  
Seminal Plasma Proteins ◽  
Ram Sperm

Ejaculation results in the confluence of epididymal spermatozoa with secretions of the accessory sex glands. This interaction is not a prerequisite for fertilisation success, but seminal factors do play a crucial role in prolonging the survival of spermatozoa bothin vitroandin vivoby affording protection from handling induced stress and some selective mechanisms of the female reproductive tract. Reproductive biologists have long sought to identify specific factors in seminal plasma that influence sperm function and fertility in these contexts. Many seminal plasma proteins have been identified as diagnostic predictors of sperm function and have been isolated and appliedin vitroto prevent sperm damage associated with the application of artificial reproductive technologies. Proteomic assessment of the spermatozoon, and its surroundings, has provided considerable advances towards these goals and allowed for greater understanding of their physiological function. In this review, the importance of seminal plasma will be examined through a proteomic lens to provide comprehensive analysis of the ram seminal proteome and detail the use of proteomic studies that correlate seminal plasma proteins with ram sperm function and preservation ability.

Functional attributes of seminal proteins in bull fertility: A systematic review

Reproduction ◽  
2021 ◽  
Author(s):  
Arabela Guedes de Azevedo Viana ◽  
Iara Magalhães Ribeiro ◽  
Renner Philipe Rodrigues Carvalho ◽  
Erdogan Memili ◽  
Arlindo Alencar Moura ◽  
...  
Keyword(s):  
Systematic Review ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Protein Isoforms ◽  
Heparin Binding ◽  
Protein Biomarkers ◽  
Functional Attributes ◽  
Fertilizing Ability ◽  
Seminal Plasma Proteins ◽  
Sperm Fertilizing Ability

Proteomic approaches have been widely used in reproductive studies to uncover protein biomarkers of bull fertility. Seminal plasma is one of the most relevant sources of these proteins that may influence sperm physiology. Nonetheless, there are still gaps in existing knowledge in the functional attributes of seminal proteins. Thus, we reviewed the relationships between seminal plasma proteins and bull fertility by conducting a systematic review with data obtained from 71 studies. This review showed that the associations between fertility improvement with the use of total seminal plasma proteins are still controversial. None of the studies explored the sperm fertilizing ability following these interactions. By contrast, the exposure to a single protein, such as osteopontin, binder of sperm proteins, and heparin binding proteins, can increment sperm motility, capacitation, and fertilizing ability by modulating intracellular calcium concentrations, removing lipids from sperm membranes, and regulating the acrosome reaction. Variations in protein analyses and the protein contents and their abundances between animals contributed to the difficulty of establishing protein biomarkers of fertilizing potential of the bull sperm. Indeed, the heterogenicity of methodologies was a limitation of this review. Standardized methods of seminal protein analyses, as well as sperm endpoints, may minimize such discrepancies. In conclusion, potential biomarkers of sperm parameters are still to be established. Future studies should evaluate protein isoforms and how they interact with sperm to ascertain their biological functions.

scholarly journals Boar seminal plasma proteins and their relevance to reproductive technologies

2019 ◽  
Author(s):  
W.L. Flowers ◽  
K.R. Stewart ◽  
T. Gal ◽  
S. Novak ◽  
M.K. Dyck ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Reproductive Technologies ◽  
Seminal Plasma Proteins

scholarly journals 27 Shotgun proteomics reveal seminal plasma proteomes are reflective of boar reproductive performance

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 115-115
Author(s):  
Kayla M Mills ◽  
Uma Aryal ◽  
Tiago Sobreira ◽  
Amanda Minton ◽  
Theresa Casey ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Seminal Plasma ◽  
Reproductive Performance ◽  
Plasma Proteins ◽  
Proteome Analysis ◽  
Shotgun Proteomics ◽  
High Fertility ◽  
Protein Profiles ◽  
Seminal Plasma Proteins ◽  
Farrowing Rate

Abstract Subfertile boars have a negative economic impact for producers and need to be identified before they enter the breeding herd. Current semen evaluation techniques are not able to identify all subfertile boars. Seminal plasma proteins are essential for normal sperm function and transport and play an important role in fertilization. We hypothesize that seminal plasma proteins affect fertility, and thus differences in protein profiles reflect differences in boar fertility. The objective of this study was to use LC-MS/MS for shotgun proteome analysis to investigate whether differences in boar fertility phenotype can be differentiated by seminal plasma protein expression. Following 50 single-sire matings, boars were categorized into one of four phenotypes: high farrowing rate and high total born (HFHB; n=9), high farrowing rate with low total born (HFLB; n=10), low farrowing rate and low total born (LFLB; n=9), and low farrowing rate with high total born (LFHB; n=4). There were 436 proteins measured in at least one sample across all animals. There were 245 high confidence proteins and 56 were differentially abundant (P < 0.05) between the high fertility phenotype (HFHB) and at least one of the three subfertile groups. Proteins associated with fertility and free radical detoxification were more abundant in HFHB whereas subfertile phenotypes had a greater abundance of blood microparticle proteins, biomarkers of inflammation, and IMPA1, which regulates inositol production. Findings support that seminal plasma protein profiles are distinct between boars with different fertility phenotypes and have the potential to predict boar reproductive performance.

Genetic Polymorphism of Boar Seminal Plasma Proteins

1993 ◽  
Vol 64 (3) ◽  
pp. 221-227
Author(s):  
Soichi TSUJI ◽  
Masatoshi ASAO ◽  
Hiroshi KUSUNOKI ◽  
Takao OISHI
Keyword(s):  
Genetic Polymorphism ◽  
Seminal Plasma ◽  
Plasma Proteins ◽  
Seminal Plasma Proteins

Seasonal differences in seminal plasma proteins from two bovine breeds adapted to a subtropical climate

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Paula Lorena Souto ◽  
Lilian Silveira Travassos Carmouy ◽  
Cristiane Santos ◽  
Edison Martins ◽  
Vera Martins ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Subtropical Climate ◽  
Seasonal Differences ◽  
Seminal Plasma Proteins

scholarly journals Seminal Plasma Proteins Revert the Cold-Shock Damage on Ram Sperm Membrane1

2000 ◽  
Vol 63 (5) ◽  
pp. 1531-1537 ◽  
Author(s):  
Beatriz Barrios ◽  
Rosaura Pérez-Pé ◽  
Margarita Gallego ◽  
Agustín Tato ◽  
Jesús Osada ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Cold Shock ◽  
Seminal Plasma Proteins ◽  
Ram Sperm
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