186 QUANTITATIVE ECHOGENICITY AND ECHOTEXTURE ANALYSIS OF THE ACCESSORY SEX GLANDS OF PUBERTAL AND MATURE DORPER RAMS

2016 ◽  
Vol 28 (2) ◽  
pp. 224
Author(s):  
E. S. C. Camela ◽  
R. P. Nociti ◽  
V. J. C. Santos ◽  
B. I. Macente ◽  
G. S. Maciel ◽  
...  
Keyword(s):  
Image Analysis ◽  
Sexual Maturity ◽  
Calculated Data ◽  
Tukey Test ◽  
Statistical Procedures ◽  
Accessory Sex Glands ◽  
Linear Transducer ◽  
Computerised Image Analysis ◽  
Levene Test ◽  
Transducer Frequency

This study aimed to analyse the ultrasonographic attributes of vesicular, prostate, and bulbourethral glands in pubertal and mature Dorper rams. Forty-five rams were used in the same day (pubertal: 8–11 months, n = 24; mature: ≥12 months, n = 21). The B-mode ultrasounds examinations were performed using MyLab 30Vet equipment (Esaote, Naples, Italy) connected to transrectal linear transducer (frequency of 7.5 MHz). The echogenicity [(mean numerical pixel values (NPV)] and pixel heterogeneity (standard deviation of NPV) of accessory sex glands parenchymas was determined by computerised image analysis using Image ProPlus® software (Media Cybernetics Inc., Rockville, MD, USA). For the pairs organs a mean for each parameter were calculated. Data were analysed by ANOVA with Tukey test (mean ± s.d.; P < 0.05), after the normality and homoscedasticity of residuals were checked with Shapiro-Wilk test and Levene test, respectively, when necessary log-transformation was applied. All statistical procedures was performed with R® software. The echogenicity of parenchymas were different (P < 0.05) between pubertal and mature rams for vesicular glands (181.51 ± 20.80 v. 164.83 ± 26.79) and bulbourethral glands (166.93 ± 16.93 v. 141.80 ± 29.15); however, there was no difference (P > 0.05) for prostate glands (99.39 ± 36.34 v. 87.47 ± 34.24). The pixel heterogeneity did not differ (P > 0.05) between pubertal and mature rams (29.06 ± 2.67 v. 28.09 ± 3.95, 32.14 ± 8.20 v. 30.28 ± 4.71, and 27.82 ± 4.53 v. 28.74 ± 4.29) for vesicular, prostate, and bulbourethral glands, respectively. In conclusion, the sexual maturity only influenced the echogenicity of vesicular and bulbourethral parenchymas of Dorper rams.

22 DYNAMIC OF SYNCHRONIZED FOLLICULAR WAVE IN EWES SUBJECTED TO DIFFERENT DOSES OF 17β-OESTRADIOL GIVEN AT THE BEGINNING OF THE PROGESTERONE PROTOCOL

2017 ◽  
Vol 29 (1) ◽  
pp. 118
Author(s):  
M. E. F. Oliveira ◽  
R. P. Nociti ◽  
E. S. C. Camela ◽  
L. C. Padilha-Nakaghi ◽  
G. S. Maciel ◽  
...  
Keyword(s):  
Financial Support ◽  
Analysis Data ◽  
Maximum Diameter ◽  
Growing Period ◽  
Follicular Wave ◽  
Tukey Test ◽  
Linear Transducer ◽  
Follicle Diameter ◽  
Transducer Frequency ◽  
Different Doses

This study was designed to evaluate the effect of different doses of 17β-oestradiol injection at the beginning of the progesterone protocol on follicular wave dynamic in ewes. In a random day of the oestrous cycle (D0), twenty-four Santa Ines ewes received an intravaginal progesterone device (CIDR®) and a injection of 17β-oestradiol (E2, Sincrodiol®, Ourofino, Brazil) in different doses (350, 500, and 1000 µg) for G-350E2, G-500E2, and G-1000E2, respectively (n = 8 per group). Ultrasound examinations were performed daily during the CIDR permanence (10 days) using MyLab 30Vet equipment (Esaote, Italy) connected to transrectal linear transducer (frequency of 7.5 MHz). Follicular wave was defined as a follicle or a group of follicles 2 to 3 mm in diameter that grew to ≥4.5 mm in size before regression or ovulation. The day of wave emergence was based on the day on which the largest follicle of a wave was first detected at 2 or 3 mm (retrospective analysis). Data were analysed by ANOVA followed by a Tukey test (mean ± SEM; P < 0.05) using SAS software. The emergence of a new follicular wave after the beginning of protocols was not detected in 8 out of 24 ewes (33.3%), being 3 from G-350E2, 2 from G-500E2, and 3 from G-1000E2. There was difference (abP = 0.04) for follicular wave emergence day (3.00 ± 0.32b, 4.00 ± 0.45ab and 5.20 ± 0.73a) and averaged day of maximal follicle diameter (8.20 ± 0.58b, 9.50 ± 0.34ab and 9.80 ± 0.20a) for G-350E2, G-500E2 , and G-1000E2, respectively. However, the growing period and the maximum diameter of the largest follicle of this wave did not differ between groups (124.80 ± 8.98 h and 5.42 ± 0.25 mm for G-350E2; 132.00 ± 5.37 h and 5.75 ± 0.23 mm for G-500E2; and 110.40 ± 16.28 h and 5.20 ± 0.73 mm for G-1000E2, respectively). In conclusion, the 17β-oestradiol injection at the beginning of the progesterone protocol was not able to induce the emergence of a new follicular wave in all of females, regardless of dose. However, the synchronized follicular wave occurred earlier in females treated with the lowest dose of the 17β-oestradiol than those that received the highest dose. The study received financial support from CNPq and FAPESP.

Interaction of Neutrophils with Endothelial Cells, Fibroblasts and Their Extracellular Matrices: Microscopic and Computerised Analysis

1988 ◽  
Vol 16 (1) ◽  
pp. 48-53
Author(s):  
Marina Ziche ◽  
Lucia Morbidelli ◽  
Annalisa Rubino ◽  
Piero Dolara ◽  
Stefano Bianchi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Image Analysis ◽  
Vascular Endothelial Cells ◽  
Extracellular Matrices ◽  
Polymorphonuclear Neutrophil ◽  
Initial Event ◽  
Computerised Image Analysis ◽  
Capillary Endothelial Cells ◽  
Vitro Model

Polymorphonuclear neutrophil (PMN) interaction with vascular endothelial cells is the initial event in the migration of neutrophils through blood vessel walls before reaching inflammation sites in tissues. The interaction between fibroblasts and endothelial cells and their extracellular matrices might be modulated by the activation of neutrophils that occurs at inflammatory reaction sites. We have used an in vitro model to study PMN function, measuring the adhesion of human PMNs to capillary endothelial cells and fibroblasts grown in culture and to their extracellular matrices. The interaction was measured in basal conditions and in the presence of the chemotactic effector, formyl-methionyl-leucyl-phenylalanine (FMLP at the concentration of 10 7M). Adhesion was expressed by the number of adherent PMNs/mm2 on a histological specimen. Moreover, we have adapted a program for image analysis to quantify neutrophil adhesion. Three times more PMNs adhered to matrices than to monolayers, and adherence could be increased by the presence of 10-7M FMLP, except in the case of fibroblast monolayers. We found a good correlation between microscopic observation and computerised image analysis measuring PMN adhesiveness to extracellular matrices.

Computerised Image Analysis of Nuclear Enlargement Assay in HeLa Cells

1991 ◽  
Vol 19 (1) ◽  
pp. 41-47
Author(s):  
Renato Rizzi ◽  
Francesco Re ◽  
Enzo Chiesara
Keyword(s):  
Image Analysis ◽  
Hela Cells ◽  
Automatic Analysis ◽  
Nuclear Size ◽  
Computerised Image Analysis ◽  
Nuclear Enlargement

It has been observed that cells often respond to carcinogens by nuclear enlargement. For this reason, new morphometric approaches have been developed to evaluate cell modifications in pre-carcinogenesis assays. Morphometric computerised automatic analysis, with original software, was performed on HeLa cells treated with various compounds (hydroxyurea, dimethylnitrosamine, N-methyl- N’-nitro-nitrosoguanidine and cyclophosphamide) to evaluate nuclear size changes.

Quantification and enumeration of cytokine producing cells by computerised image analysis

Cytokine ◽  
1994 ◽  
Vol 6 (5) ◽  
pp. 539
Author(s):  
Lars Björk ◽  
Ulf Andersson ◽  
Jan Andersson
Keyword(s):  
Image Analysis ◽  
Computerised Image Analysis

Reproductive characteristics of wild native Australian Rattus (Rodentia : Muridae)

1973 ◽  
Vol 21 (4) ◽  
pp. 437 ◽  
Author(s):  
JM Taylor ◽  
BE Horner
Keyword(s):  
Sexual Maturity ◽  
Reproductive Potential ◽  
Field Conditions ◽  
Museum Specimens ◽  
Seasonal Breeding ◽  
Reproductive Characteristics ◽  
Accessory Sex Glands

Reproduction in field populations of the five species and 13 of 14 subspecies of native Australian Rattus is compared, using both materials personally collected and museum specimens. We have separated each subspecies into juvenile and adult categories, the dividing point reflecting the approximate size at which sexual maturity is reached. Occurrence of pregnancies and juveniles, treated monthly, permit estimates of time and length of seasonal breeding for each subspecies. Information on gonads and accessory sex glands is given for both breeding and non-breeding adults and for juveniles. All Australian subspecies of Rattus have a fundamentally similar mechanism of reproduction. Those attaining high densities have the highest ovulation rates, the largest litter sizes, and the greatest number of teats. Furthermore, in the two subspecies of high reproductive potential for which we have evidence, females can be precocious in attaining sexual maturity under field conditions.

Sign in / Sign up

Export Citation Format

Share Document