176 SERUM ANTIMULLERIAN HORMONE CONCENTRATIONS AND IN VITRO EMBRYO PRODUCTION IN BEEF CATTLE

2016 ◽  
Vol 28 (2) ◽  
pp. 219
Author(s):  
G. A. Bó ◽  
I. Anduaga ◽  
B. Bernal ◽  
G. Moreira ◽  
A. Cedeño ◽  
...  
Keyword(s):  
Pearson Correlation ◽  
Oestrous Cycle ◽  
Serum Concentrations ◽  
Embryo Production ◽  
Antral Follicles ◽  
Antimullerian Hormone ◽  
In Vitro Embryo Production ◽  
Antimüllerian Hormone ◽  
Follicle Aspiration

The collection of high numbers of oocytes for in vitro embryo production in the cow depends on the number of antral follicles available for aspirations. The number of antral follicles varies significantly among cows and can be estimated by ovarian ultrasonography or measurement of serum concentrations of antimullerian hormone (AMH). The AMH is secreted by the granulosa cells of small antral follicles and serum concentrations are independent of the stage of the oestrous cycle. Therefore, it may be useful as a tool to select oocyte donors for in vitro embryo production. An experiment was designed to evaluate the effect of breed of cattle and the correlation of serum AMH concentrations on the number of viable oocytes and embryos produced in vitro. Cumulus-oocyte complex were collected during 44 ultrasound-guided follicle aspiration sessions performed at random stages of the oestrous cycle without superstimulation in 14 Bonsmara, 11 Braford, and 19 Brangus cows that were used for commercial in vitro embryo production using frozen-thawed semen from bulls of the same breed. Blood samples were collected by jugular venipuncture at the time of OPU, centrifuged at 3000 × g for 30 min for serum separation which was frozen at –20°C until AMH analysis. Serum AMH was evaluated using an ELISA (AnshLabs, Webster, TX, USA). The sensitivity of the AMH assay was 0.011 ng mL–1 and intra-assay CV were <5%. The number of viable oocytes collected and embryos produced in each breed were compared by ANOVA and means were compared by the protected l.s.d. test when ANOVA revealed statistically significant differences. Regression analysis and Pearson correlation were used to determine the relationship between the number of viable oocytes aspirated and the number of embryos produced. The mean (± SEM) number of viable oocytes collected was greater (P < 0.05) from Brangus (23.3 ± 4.3) and Braford (22.5 ± 3.6) than from Bonsmara donors (12.2 ± 2.5). Although not statistically different, the numbers of embryos produced followed the same pattern (Brangus, 4.4 ± 0.9; Braford, 3.4 ± 0.6; Bonsmara, 2.9 ± 0.5). Serum AMH concentration was positively correlated with the number of viable oocytes collected in all breeds (R2 = 0.63 for Bonsmara, 0.50 for Braford, 0.32 for Brangus; P < 0.01). Serum AMH concentrations were also correlated with the number of embryos produced in all breeds (R2 = 0.32 for Bonsmara, 0.16 for Braford, 0.39 for Brangus). However, AMH concentrations were not significantly correlated with the proportion of viable oocytes collected and embryos produced. Results demonstrate a breed effect on the number of oocytes collected per session and that circulating AMH concentrations are correlated with the number of viable oocytes collected per session in commercial in vitro embryo production programs.

231 EFFECTS OF DIETARY PROPYLENE GLYCOL ON FOLLICULAR FLUID AND OVUM PICK UP IN VITRO EMBRYO PRODUCTION IN GROWTH-RESTRICTED HEIFERS WITH TWO PROFILES OF ANTI-MÜLLERIAN HORMONE

2015 ◽  
Vol 27 (1) ◽  
pp. 205
Author(s):  
G. Gamarra ◽  
C. Ponsart ◽  
S. Lacaze ◽  
B. Le Guienne ◽  
P. Humblot ◽  
...  
Keyword(s):  
Propylene Glycol ◽  
Follicular Fluid ◽  
Plasma Concentrations ◽  
Oestrous Cycle ◽  
Water Control ◽  
Embryo Production ◽  
Blood Samples ◽  
Metabolic Hormones ◽  
In Vitro Embryo Production

Fertility and embryo quality can be improved in cattle by using diets that induce a programmed modulation of circulating insulin concentrations. The aim of this study was to test whether the daily oral administration of propylene glycol (PG) could modify metabolite and hormone plasma and follicular fluid concentrations and improve in vitro embryo production in superovulated growth-restricted heifers (600 g day–1). Sixteen Holstein heifers were grouped according to their pre-experimental anti-Müllerian hormone (AMH) plasma concentrations: low (L = 1–80 pg mL–1; n = 7) or high (H: >150 pg mL–1; n = 9). Heifers received a single daily drench from Day 1 to Day 9 of an oestrous cycle [first cycle, 400 mL of water (control) and second cycle, 400 mL of PG]. Serial jugular blood samples were collected on Day 7 of each cycle to monitor plasma insulin, glucose, and β-hydroxybutyrate (BHB) concentrations in relation to the drench. Blood samples were also collected to measure insulin-like growth factor-1 (IGF1) and progesterone (P4) concentrations on Days 0, 2, 5, 7, and 9 of the oestrous cycle. Follicular fluid was collected on Day 9 to measure insulin and IGF1 concentrations. Ovarian ultrasonography was performed on Days 2 and 5 to count follicles between 2 and 8 mm in diameter and estimate their size. After ovum pickup (OPU) performed following superovulation on Day 5 of the oestrous cycle, oocytes were matured and fertilized in vitro, then embryos were cultured for 7 days. Propylene glycol increased plasma concentrations of insulin and glucose and reduced BHB in both groups of heifers compared with control. It also increased IGF1 concentrations on Days 5 and 7 in AMH L heifers and on Days 2, 5, and 7 in AMH H heifers, and reduced P4 concentrations on Days 5 and 9 of the oestrous cycle in all heifers. In follicular fluid, there was no difference in insulin concentrations between groups, but PG increased IGF1 concentrations in all heifers. In ovaries, PG increased the number of small follicles (2–3 mm) and total follicles on Day 2 of the cycle in all heifers, and medium follicles (4–8 mm) and total follicles on Day 5 in AMH H heifers. Propylene glycol improved the in vitro embryo development rate (total number of embryos/number of fertilized oocytes) in all heifers (AMH L: control, 37.9% v. PG, 50.0%; P < 0.05; AMH H: control, 36.4% v. PG, 48.3%; P < 0.05). In AMH H, the number of grade 1 blastocysts was increased by PG (control, 5.2 ± 1.0 v. PG, 8.9 ± 1.0; P < 0.01), whereas there was no difference between treatments in AMH L heifers (control, 1.9 ± 1.1 v. PG, 3.2 ± 1.1; P > 0.05). These results indicate that short-term oral PG supplementation affects the concentrations of metabolites and metabolic hormones in blood and IGF1 concentrations in follicular fluid. PG administration is effective in improving in vitro embryo production more markedly in heifers with high AMH compared with low AMH endocrine levels.

233 HIGH NUMBERS OF ANTRAL FOLLICLES INFLUENCE THE IN VITRO EMBRYO PRODUCTION, BUT NOT THE CONCEPTION RATE OF FIXED-TIME ARTIFICIAL INSEMINATION IN NELORE CATTLE

2015 ◽  
Vol 27 (1) ◽  
pp. 206
Author(s):  
G. M. G. Santos ◽  
K. C. Silva-Santos ◽  
T. R. R. Barreiros ◽  
F. Morotti ◽  
B. V. Sanches ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Device Removal ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Antral Follicles ◽  
Conception Rates ◽  
In Vitro Embryo Production ◽  
Embryo Grade

The aim of this study was to compare the conception rates to fixed-time artificial insemination (FTAI) and in vitro embryo production between Nelore cows with high or low antral follicle counts (AFC). First, multiparous Nelore cows (Bos indicus, n = 547, 40–60 days postpartum) were subjected to synchronization of ovulation. Randomly during their oestrous cycle (Day 0), cows received an intravaginal device containing 1.9 g of P4 (CIDR®) and 2 mg of oestradiol benzoate (Estrogin®), intramuscularly. At device removal (Day 8), cows received 500 µg of PGF2α (Ciosin®), 300 IU of eCG (Novormon®), and 1 mg of oestradiol cipionate (ECP®), intramusculary. All cows were inseminated 48 h after P4 device removal. Antral follicles = 3 mm were counted using an intravaginal microconvex transducer (Day 0), and cows were assigned to groups of high (G-High, = 25 follicles, n = 183), intermediate (G-Intermediate, 16–20 follicles, n = 183), or low AFC (G-Low, = 10 follicles, n = 181). In another study to compared the in vitro embryo production, Nelore cows (n = 66, 72–96 months) were subjected to ultrasound-guided follicular aspiration using an intravaginal microconvex array transducer (7.5 MHz). The COC were selected and cows were assigned to groups according to the oocyte production: G-High (n = 22, = 40 oocytes), G-Intermediate (n = 25, 18–25 oocytes), or G-Low (n = 19, = 7 oocytes). Previously tested semen from a single bull was used for IVF using a previously described protocol (Silva-Santos et al. 2014 Reprod. Domest. Anim. 49, 228–232). The oocyte and embryo production (viable embryo: grade I, II, III; vitrifiable embryo: grade I, II) were evaluated. The number of follicles was evaluated by Kruskal-Wallis, and the chi-square test was used for data on oocyte and embryo production (P = 0.05). The average follicular population was 30.7 ± 5.7 (G-High), 18.6 ± 1.64 (G-Intermediate), and 7.8 ± 2.4 follicles (G-Low; P < 0.05), but there were no differences in the conception rates among groups (51.9 v. 48.6 v. 58.6%, respectively; P > 0.05). The total number of oocytes recovered were 1109 (G-High), 534 (G-Intermediate), and 101 (G-Low; P < 0.05). The mean number of viable oocytes was 40.4 ± 10.6 (G-High), 14.8 ± 3.0 (G-Intermediate), and 3.8 ± 1.1 (G-Low; P < 0.05) and the percentage of viable oocytes was 80% (G-High), 69% (371/534, G-Intermediate), and 71% (G-Low; P < 0.05). Cleavage rate was 79% (G-High), 74% (348/472, G-Intermediate), and 71% (G-Low; P < 0.05), and blastocyst rate was 42% (G-High), 32% (153/472, G-Intermediate), and 13% (G-Low; P < 0.05). The number of viable embryos was 18.4 ± 6.7 (G-High), 6.1 ± 3.6 (G-Intermediate), and 0.6 ± 0.7 (G-Low; P < 0.05) and the percentage of vitrifiable embryos was 81% (G-High), 77% (118/153, G-Intermediate), and 58% (G-Low; P < 0.05). Therefore, Nelore cows with high oocyte production had ~10-fold higher oocyte production and produced ~30-fold more embryos compared with the low AFC group. In conclusion, AFC had no influence on the conception rates to FTAI; however, Nelore cows with high oocyte production exhibited higher in vitro embryo production.

143 Effect of corpus luteum on recovery rate, quality, and in vitro developmental competence of oocytes in Bos indicus dairy cows

2019 ◽  
Vol 31 (1) ◽  
pp. 196
Author(s):  
M. Saad ◽  
Z. Sarwar ◽  
M. Saleem ◽  
U. Arshad ◽  
M. Shahzad ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Recovery Rate ◽  
Bos Indicus ◽  
Developmental Competence ◽  
Serum Concentrations ◽  
Embryo Production ◽  
The Mean ◽  
In Vitro Embryo Production

Assisted reproductive technologies have modernized the dairy industry for the rapid multiplication of superior genetic traits. However, the exploitation of genetics through in vitro embryo production in farm animals has been struggling during the last 3 decades. The presence or absence of a corpus luteum (CL) on ovaries from which cumulus-oocyte complexes were recovered has been shown to affect the quality of oocytes and in vitro embryo production outcome in Bos taurus cows. Similar information is lacking in Bos indicus cows. Therefore, the objectives of the present study were to determine the effect of presence or absence of a CL on recovery rate, quality, and in vitro developmental competence of oocytes in Bos indicus dairy cows. This study was conducted from December 2017 to April 2018. The ovaries were harvested from a local abattoir (Bos indicus; 5- to 8-year-old cows having mixed parity with clinically normal reproductive tracts). These ovaries (n=750) were divided into 2 groups [(1) CYCLIC (n=318) and (2) ACYCLIC (n=432)] based on the presence or absence of mature CL having follicles on either left or right ovaries of slaughtered cows. Blood samples from the jugular vein were collected at slaughter. Serum concentrations of progesterone of CYCLIC and ACYCLIC cows were measured using radioimmunoassay. Mean number of recovered oocytes per ovary and serum concentration of progesterone were compared using PROC TTEST model. However, quality, maturation, cleavage, 8-cell, 16-cell, and morula rates were analysed by PROC FREQ model of Chi-squared. All the data was analysed using SAS (SAS Institute Inc., Cary, NC, USA). Results revealed that the mean serum concentrations of progesterone (4.21±0.4v. 0.5±0.2ng mL−1; P&lt;0.05) were higher in CYCLIC as compared with ACYCLIC cows, respectively. The mean number of oocytes recovered per ovary (6.5±4.5v. 4.0±4.4; P&lt;0.05) was higher in CYCLIC than in ACYCLIC cows. The oocytes with grade I+II quality (55.3v. 47.6%; P&lt;0.05) were higher, whereas oocytes with grade III+IV quality (44.5v. 52.4%; P&lt;0.05) were lower, in CYCLIC as compared with ACYCLIC cows, respectively. Furthermore, cleavage rate (70.9v. 52.8%; P&lt;0.05) was higher in CYCLIC than in ACYCLIC cows, respectively. Similarly, the 8-cell (38.5v. 20.8%; P&lt;0.05) and 16-cell (20.0v. 10.9%; P&lt;0.05) stage embryos were greater in CYCLIC as compared with ACYCLIC cows, respectively. In conclusion, the presence of CL has a beneficial effect on recovery rate, quality, and in vitro embryo production outcomes in Bos indicus dairy cows. It is implied that for ovum pickup, cyclic cows should be preferred.

scholarly journals Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Francisco A. García-Vázquez ◽  
Carla Moros-Nicolás ◽  
Rebeca López-Úbeda ◽  
Ernesto Rodríguez-Tobón ◽  
Ascensión Guillén-Martínez ◽  
...  
Keyword(s):  
Western Blot ◽  
Female Genital Tract ◽  
Oestrous Cycle ◽  
Control Group ◽  
Blastocyst Development ◽  
Embryo Production ◽  
Or Efficiency ◽  
In Vitro Embryo Production ◽  
Proteomic Analyses

AbstractRecent evidence supports involvement of the acute phase protein haptoglobin in numerous events during mammalian reproduction. The present study represents an in-depth investigation of haptoglobin expression and secretion in the porcine oviduct and uterus, and assesses its effect on porcine in vitro embryo production. A systematic study was made of sows in different oestrous stages: late follicular, early luteal and late luteal stages. Relative haptoglobin mRNA abundance was quantified by RT-qPCR. In addition, expression of the protein was analysed by immunohistochemistry and the results were complemented by Western-blot and proteomic analyses of the oviductal and uterine fluids. In vitro porcine fertilization and embryo culture were carried out in the presence of haptoglobin. The results indicate that haptoglobin mRNA expression in the porcine oviduct and uterus is most abundant during the late luteal stage of the oestrous cycle. By means of Western blot and proteomic analyses haptoglobin presence was demonstrated in the oviduct epithelium and in the oviductal and uterine fluids in different stages of the oestrous cycle. The addition of haptoglobin during gamete co-incubation had no effect on sperm penetration, monospermy or efficiency rates; however, compared with the control group, blastocyst development was significantly improved when haptoglobin was present (haptoglobin: 64.50% vs. control: 37.83%; p < 0.05). In conclusion, the presence of haptoglobin in the oviduct and uterus of sows at different stages of the oestrous cycle suggests that it plays an important role in the reproduction process. The addition of haptoglobin during in vitro embryo production improved the blastocyst rates.

244 IN VITRO EMBRYO PRODUCTION WITH CONVENTIONAL OR Y-SEXED SEMEN IN BEEF CATTLE

2015 ◽  
Vol 27 (1) ◽  
pp. 211 ◽  
Author(s):  
H. E. Tribulo ◽  
J. Carcedo ◽  
R. J. Tribulo ◽  
B. Bernal ◽  
J. Garzon ◽  
...  
Keyword(s):  
Mixed Models ◽  
Random Variable ◽  
In Vitro Production ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Sexed Semen ◽  
The Mean ◽  
In Vitro Embryo Production ◽  
Follicle Aspiration

An experiment was designed to evaluate in vitro embryo production following the use of frozen-thawed conventional or Y-sexed semen from a single Brangus and a single Braford bull of proven fertility. Semen was obtained by splitting the same ejaculate to be frozen directly or sex-sorted and then frozen. Oocytes were obtained from 69 ultrasound-guided follicle aspiration (ovum pickup) sessions performed at random stages of the oestrous cycle without superstimulation in 24 Brangus and 10 Braford cows and heifers. Viable oocytes (n = 1120) were matured in TCM-199 medium with NaHCO3 and supplemented with 1% fetal bovine serum. Frozen-thawed sperm from the Brangus and Braford bulls were selected with Percoll for IVF, capacitated in Fert Medium, and used at a final concentration of sperm per milliliter for conventional (non-sexed) semen and 2 × 106 sperm mL–1 for Y-sexed semen. After 16 h (sexed) or 18 h (conventional) of co-incubation with oocytes in Fert Medium, presumptive zygotes were denuded and cultured in SOF supplemented with 0.4% BSA under oil at 37°C, 5% CO2, and saturated humidity for 7 days. The total number of oocytes matured and fertilized from the Brangus donors was 538 and 318 for conventional and sexed semen, respectively. The total numbers of oocytes matured and fertilized from the Braford donors were 139 and 125 for conventional and sexed semen, respectively. Data were compared by ANOVA for mixed models, using breed and type of semen as fixed variables and cow (i.d.) as a random variable. Cleavage and blastocyst rates were first transformed by square root and then analysed by ANOVA for mixed models. Mean (± s.e.m.) number of total viable oocytes collected, cleaved zygotes, and blastocysts produced per ovum pickup session did not differ (P = 0.18) between breeds (Brangus: 17.1 ± 1.6, 10.0 ± 0.9, and 6.2 ± 0.7 v. Braford: 13.9 ± 2.8, 7.6 ± 1.5, and 4.0 ± 0.8), and there was no breed × semen interaction on the mean number of cleaved zygotes and blastocysts produced. However, the mean (± s.e.m.) number of cleaved zygotes and blastocysts produced was significantly higher (P < 0.05) when the oocytes were fertilized with conventional semen (10.7 ± 1.2 and 6.5 ± 0.8) than with sexed semen (7.7 ± 0.7 and 4.3 ± 0.6). The mean cleavage rate was also significantly higher (P < 0.05) when the oocytes were fertilized with conventional semen (76.8 ± 3.9) than with sexed semen (54.1 ± 4.2). Blastocyst rate tended to be higher (P = 0.1) with conventional semen (40.5 ± 3.3) than with sexed semen (33.6 ± 4.2). Although in vitro production may be the preferred alternative for the production of embryos of a known sex, the number of blastocysts produced might be reduced as compared with the use of non-sexed semen from the same bull.

246 INFLUENCE OF BREED AND SEASON ON IN VITRO EMBRYO PRODUCTION

2016 ◽  
Vol 28 (2) ◽  
pp. 255
Author(s):  
B. Bernal ◽  
J. Revol ◽  
J. M. Oviedo ◽  
A. Tribulo ◽  
H. Tribulo ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
In Vitro Production ◽  
Embryo Production ◽  
Significant Difference ◽  
In Vitro Embryo Production ◽  
Follicle Aspiration ◽  
Vitro Production ◽  
Difference Test

A retrospective analysis of in vitro production (IVP) data was done to determine the influence of breed and season on the production of viable oocytes and embryos. Cumulus‐oocyte complexes (COC) were obtained from 1946 ultrasound-guided follicle aspiration (ovum pickup) sessions performed at random stages of the oestrous cycle without superstimulation in Bos taurus and Bos indicus donors in commercial IVP in Argentina. Frozen-thawed conventional semen was used in beef cattle and conventional (n = 139) and sexed-selected (n = 481) semen in dairy cattle. The COC were classified, matured in B-199 medium, fertilized in IVF-SOF medium (Day 0), and cultured in SOF medium supplemented with 0.4% BSA under oil at 38.8°C, 5.5% CO2, and saturated humidity for 7 days. The number of viable COC and transferable embryos in each breed and season were compared by ANOVA and means were compared by Fisher’s Least Significant Difference test. Proportions were first transformed by arcsin and then analysed by ANOVA. To simplify the interpretation of the results, breeds were grouped as follows: dairy Bos taurus (Holstein, n = 620), beef Bos taurus (Angus and Bonsmara, n = 229), Bos taurus × Bos indicus (Brangus and Braford, n = 1045), and Bos indicus (Brahman, n = 52). There was no interaction between breed and season for any of the end points analysed (P > 0.1). Mean (± standard error of the mean) numbers of viable COC and transferable embryos were higher (P < 0.01) in Bos indicus × Bos taurus (19.3 ± 0.4 and 5.3 ± 0.2, respectively) and Bos indicus (15.8 ± 1.4 and 6.8 ± 0.9, respectively) than in beef (11.6 ± 0.5 and 3.0 ± 0.2, respectively) and dairy (8.0 ± 0.2 and 1.6 ± 0.1, respectively) Bos taurus donors. Cleavage rates were higher (P < 0.01) in Bos indicus (72%) than in the other breeds (57% for Bos indicus × Bos taurus and dairy Bos taurus and 54% for beef). Transferable embryo rates were higher (P < 0.01) in Bos indicus (41%) and Bos indicus × Bos taurus (30%) than in beef Bos taurus (26%). Dairy Bos taurus had the lowest (P < 0.01) embryo rates of all breeds (21%). In dairy Bos taurus, cleavage rates, the number of embryos produced, and transferable embryo production rates were higher (P < 0.01) when conventional semen was used (62%, 2.8 ± 0.15, and 27%, respectively) compared to sexed-selected semen (55%, 1.3 ± 0.1, and 19%, respectively). With regards to season, the number of viable COC was highest (P < 0.01) in the spring (14.3 ± 0.5), lowest in the summer (11.3 ± 1.0), and intermediate in the fall (12.2 ± 1.2) and winter (13.7 ± 1.2), which did not differ. Although not affected significantly by season, the number of embryos produced was numerically lower in the summer (2.8 ± 0.4) than in the spring (4.2 ± 0.2), winter (4.5 ± 0.5), or fall (4.6 ± 0.5). In conclusion, in vitro embryo production was directly influenced by breed and season. Bos indicus influenced cattle and the spring season were preferable for commercial IVP programs that did not include superstimulation.

High numbers of antral follicles are positively associated with in vitro embryo production but not the conception rate for FTAI in Nelore cattle

2016 ◽  
Vol 165 ◽  
pp. 17-21 ◽  
Author(s):  
Gustavo Martins Gomes dos Santos ◽  
Katia Cristina Silva-Santos ◽  
Thales Ricardo Rigo Barreiros ◽  
Fábio Morotti ◽  
Bruno Valente Sanches ◽  
...  
Keyword(s):  
Conception Rate ◽  
Embryo Production ◽  
Antral Follicles ◽  
In Vitro Embryo Production ◽  
Nelore Cattle

Bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration

2003 ◽  
Vol 59 (5-6) ◽  
pp. 1123-1130 ◽  
Author(s):  
Gianluca Neglia ◽  
Bianca Gasparrini ◽  
Viviana Caracciolo di Brienza ◽  
Rossella Di Palo ◽  
Giuseppe Campanile ◽  
...  
Keyword(s):  
Embryo Production ◽  
In Vitro Embryo Production ◽  
Follicle Aspiration

Likelihood of pregnancy after the transfer of embryos derived from follicle aspiration and in vitro embryo production sessions with different relative efficiencies

2018 ◽  
Vol 193 ◽  
pp. 165-170 ◽  
Author(s):  
Luiz Fernando Feres ◽  
Luiz Gustavo Bruno Siqueira ◽  
Miller Pereira Palhao ◽  
Lívia Loiola dos Santos ◽  
Felipe Zandonadi Brandao ◽  
...  
Keyword(s):  
Embryo Production ◽  
In Vitro Embryo Production ◽  
Follicle Aspiration

scholarly journals Laparoscopic Ovum Pick-Up Followed by In Vitro Embryo Production and Transfer in Assisted Breeding Programs for Ruminants

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 216
Author(s):  
Hernan Baldassarre
Keyword(s):  
Pregnancy Rate ◽  
Commercial Application ◽  
Embryo Production ◽  
Breeding Programs ◽  
Sheep And Goats ◽  
Embryo Recovery ◽  
Marker Selection ◽  
Holstein Calves ◽  
In Vitro Embryo Production

The potential of laparoscopic ovum pick-up (LOPU) followed by in vitro embryo production (IVEP) as a tool for accelerated genetic programs in ruminants is reviewed in this article. In sheep and goats, the LOPU-IVEP platform offers the possibility of producing more offspring from elite females, as the procedure is minimally invasive and can be repeated more times and more frequently in the same animals compared with conventional surgical embryo recovery. On average, ~10 and ~14 viable oocytes are recovered by LOPU from sheep and goats, respectively, which results in 3–5 transferable embryos and >50% pregnancy rate after transfer. LOPU-IVEP has also been applied to prepubertal ruminants of 2–6 months of age, including bovine and buffalo calves. In dairy cattle, the technology has gained momentum in the past few years stemming from the development of genetic marker selection that has allowed predicting the production phenotype of dairy females from shortly after birth. In Holstein calves, we obtained an average of ~22 viable oocytes and ~20% transferable blastocyst rate, followed by >50% pregnancy rate after transfer, declaring the platform ready for commercial application. The present and future of this technology are discussed with a focus on improvements and research needed.

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