353 FOLLICULAR POPULATION STATUS AT THE FIRST FOLLICLE-STIMULATING HORMONE INJECTION IN EWES SUPERSTIMULATED NEAR THE FIRST FOLLICULAR WAVE OF CIDR PROTOCOL

2015 ◽  
Vol 27 (1) ◽  
pp. 264
Author(s):  
M. E. F. Oliveira ◽  
M. A. R. Feliciano ◽  
L. G. Oliveira ◽  
J. F. Fonseca ◽  
W. R. R. Vicente
Keyword(s):  
Pearson Correlation ◽  
Follicle Stimulating Hormone ◽  
Large Population ◽  
Population Status ◽  
Mean Values ◽  
Follicular Wave ◽  
Hormone Injection ◽  
Phase Data ◽  
Santa Inês ◽  
Stimulating Hormone

This study was designed to evaluate the follicular status at the 1st FSH injection in ewes superstimulated near the 1st follicular wave of the CIDR protocol during nonbreeding (NB), transition (T), and breeding (B) season, and thus to correlate them with the superovulatory response (SR). On Day 0, all females (30 Santa Ines ewes; n = 10 per season) received a progesterone (P4) device (CIDR®; Zoetis, Brazil) and 37.5 µg of d-cloprostenol. The superestimulation (ST) was initiated on Day 4, 4, and 6 of protocol in the NB, T, and B season, respectively. These follicular wave emergence days were defined in a previous study that evaluated the follicular dynamic in P4 protocol (Oliveira et al. 2011 Acta Scientiae Veterinariae, 38, 361). The ST consisted of 8 injections of pFSH administrated twice a day in descending order (40/30/20/ and 10 mg of pFSH; Folltropin-V, Bioniche, Canada). The P4 device was removed 2 days after the beginning of the FSH treatment and all ewes received another injection of 37.5 µg of d-cloprostenol and a dose of 200 IU of eCG at the same time. B-mode ultrasound of ovaries was performed immediately before the 1st FSH injection. The follicles were classified according to their diameters into categories based on physiological dynamics: (C1) 2–4.25 mm, representative of the population before dominance phase; (C2) 4.5–5 mm, initial dominance phase; (C3) 5.25–5.75 mm, middle dominance phase; and (C4) 6 mm, preovulatory phase. Data were analysed by GLIMMIX using SAS comparing mean values (± s.e.m.) between seasons (P < 0.05) and Pearson correlation was made. All ewes had small follicles (C1) at the beginning the ST; however, only one female had solely C1 follicles. The number of C1 follicles did not differ between seasons (12.9 ± 0.9, 12.8 ± 0.8, and 12.1 ± 0.5 follicles for the NB, T, and B season, respectively). One-half of the animals from NB and T seasons had no C3 and C4 follicles, whereas 40% of females in B season showed the same follicular status. The percentage of ewes that had no C4 follicles was 80, 100, and 50% for the NB, T, and B season, respectively. There were no difference between season in number of C2 follicles (1.8 ± 0.5, 1.7 ± 0.4, and 1.7 ± 0.4) and C3 follicles (0.3 ± 0.1, 0.6 ± 0.2, 0.1 ± 0.1) for NB, T, and B seasons, respectively. However, the number of C4 follicles was significantly higher (P < 0.05) in the NB (0.2 ± 0.13) and B (0.6 ± 0.2) season than T period, which had no follicles in this category. The SR did not differ between seasons (12.4 ± 0.9, 13.1 ± 2.3, and 17.0 ± 2.3 for the NB, T, and B season, respectively) and had no correlation to any follicular category. In conclusion, the ST started on a day when there was a large population of small follicles, regardless of the season, confirming proximity to follicular wave emergence. However, the ovarian population was not restricted to this category. Therefore, it is possible that wave emergence has already started in some ewes before the initiation of the ST.Financial support is from FAPESP and CNPq.

Early luteal development in Santa Inês ewes superovulated with reduced doses of porcine follicle‐stimulating hormone

2018 ◽  
Vol 54 (3) ◽  
pp. 456-463 ◽  
Author(s):  
Mariana Garcia Kako Rodriguez ◽  
Giovanna Serpa Maciel ◽  
Ricardo Andres Ramirez Uscategui ◽  
Victor José Correia Santos ◽  
Ricardo Perecin Nociti ◽  
...  
Keyword(s):  
Follicle Stimulating Hormone ◽  
Santa Inês ◽  
Stimulating Hormone

scholarly journals HORMONE LEVEL CHANGES IN ENDOMETRIAL ABNORMALITIES IN THE POSTMENOPAUSAL PERIOD

2021 ◽  
Vol 14 (5) ◽  
pp. 18-21
Author(s):  
FIDAN T. ALIEVA ◽  
◽  
DMITRY V. BRYUNIN ◽  
FARAKH T. ALIEVA ◽  
◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Emotional Disorders ◽  
Follicle Stimulating Hormone ◽  
Clinical Manifestations ◽  
Sex Hormone Binding Globulin ◽  
Diagnostic Significance ◽  
Menopausal Syndrome ◽  
Mean Values ◽  
Postmenopausal Period ◽  
Stimulating Hormone

Background. In recent decades, the increase in life expectancy has led to a significant increase in the duration of the postmenopausal period. Prolonged estrogen deficiency contributes to an increase in the incidence of chronic somatic diseases, including neurovegetative, metabolic-endocrine, and psycho-emotional disorders that define the menopausal syndrome. Aim. To study the prognostic and diagnostic significance of hormone parameters in endometrial abnormalities in the postmenopausal period. Material and methods. Forty-seven women with various clinical manifestations of endometrial pathology in the postmenopausal period were examined. The mean age of the examined women was (58,43±1,1) years. All the patients had luteinizing hormone, follicle-stimulating hormone, progesterone, testosterone, estradiol, dehydroepiandrosterone sulfate, and serum sex hormone-binding globulin measured by enzyme immunoassay. The results obtained were subjected to statistical processing using standard computer programs «Statgraph» designed for parametric and nonparametric methods of calculating mean values. We calculated mean and standard deviation (M±Se), as well as minimum-maximum values for the sample. Differences were considered significant at p<0,05. Results and discussion. As a result of the study the abnormality of the endometrium in the postmenopausal period was determined. Endometritis was observed in 4 (8,5%) of 47 patients, atrophic endometrium in 7 (14,9%) patients, diffuse endometrial hyperplasia in 3 (6,4%) cases, endometrial polyp in 29 (61,7%) cases, and endometrial carcinoma in 3 (6,4%) cases. A significant increase in follicle-stimulating hormone [(65,39±1,27) mmE/ mL], luteinizing hormone [(34,85±0,15] mmE/mL), estradiol [(88,73±2,1) pg/mL], and testosterone [(2,29±0,08) ng/mL] values was registered in the postmenopausal patients, which is an important prognostic and diagnostic criterion in this patient population. Conclusion. The study of hormone parameters in the postmenopausal period is one of the most important methods of early detection of various endometrial abnormalities, allowing timely implementation of appropriate therapeutic and preventive measures.

231 SUPEROVULATORY RESPONSE AND EMBRYO YIELD OF SANTA INES EWES SUBMITTED TO FOLLICLE STIMULATING HORMONE TREATMENTS STARTED NEAR THE TIME OF EMERGENCE OF THE FIRST AND LAST FOLLICULAR WAVE OF A PROGESTERONE-BASED PROTOCOL

2012 ◽  
Vol 24 (1) ◽  
pp. 228
Author(s):  
M. E. F. Oliveira ◽  
C. C. D'Amato ◽  
L. G. Oliveira ◽  
S. D. Bicudo ◽  
J. F. Fonseca ◽  
...  
Keyword(s):  
Buenos Aires ◽  
Animal Health ◽  
Follicle Stimulating Hormone ◽  
Corpora Lutea ◽  
Chi Square ◽  
Follicular Waves ◽  
Follicular Wave ◽  
Embryo Yield ◽  
Santa Inês ◽  
Follicular Dynamics

This study was designed to investigate if the superovulatory response and embryo yield in Santa Ines ewes are influenced by the time that FSH treatments are inititiated: near the emergence of the first or last follicular wave of a progesterone-based (P4) protocol. Days of emergence of the follicular waves was defined in a previous study that evaluated follicular dynamics in oestrus synchronization treatments (Oliveira et al. 2011 Acta Sci. Vet. 40). Twenty Santa Ines cyclic ewes were submitted to 2 superovulatory protocols according to the time that FSH treatments were initiated (G-first wave, n = 10 and G-last wave, n = 10). Thus, the FSH treatment started on Day 4 and 10 of protocol for G-first and G-last, respectively (Day 0 = onset of protocol). Ewes were treated with a P4 device (CIDR®; Pfizer-New Zealand) for 6 and 12 days for G-first and G-last, respectively. Additionally, in G-last, the CIDR was replaced by a new one on Day 7. All ewes received 2 injections of 37.5 μg of D-cloprostenol (Prolise®, Arsa-Argentina) on Day 0 and at CIDR removal. The superovulatory regimen consisted of 8 IM injections of pFSH (Folltropin®; Bioniche Animal Health, Belleville, Ontario, Canada) administrated twice daily (40, 40, 30, 30, 20, 20, 10 and 10 mg of porcine FSH). A single IM dose of 200 IU of eCG (Novormon®, Syntex, Buenos Aires, Argentina) was given concurrently with CIDR removal. Ewes were mated by a fertile ram. Embryo collections were accomplished 7 days after CIDR removal. The superovulatory response was classified in scores: (0) 4 or fewer corpora lutea (CL); (1) between 5 and 10 CL and (2) 11 or more CL. Means (± SD) were compared using Kruskal-Wallis test and percentages using Chi-square (P < 0.05). Most donors (70%, 7/10) from G-last had a superovulatory response classified as score 2 and the remainder (30%, 3/10) as score 1, whereas half of ewes from G-first were classified as score 2 and half as score 1 (P > 0.05).There was no effect between treatments (P > 0.05) for ovulation rate (G-first: 90.7 ± 10.2% vs G-last: 93.0 ± 7.2%), number of ovulations (G-first: 13.1 ± 7.3 vs G-last: 14.0 ± 5.8), or number of luteinized unovulated follicles (G-first: 1.4 ± 1.3 vs G-last: 0.9 ± 1.0). Furthermore, there was no effect between G-first and G-last (P > 0.05) in the rate of ova/embryos recovered (55.1 ± 27.1% vs 67.0 ± 19.4%), mean number of ova/embryos recovered (7.0 ± 5.5 vs 9.1 ± 3.8), number of viable embryos (3.9 ± 6.1 vs 5.7 ± 4.4), or viability rate (49.1 ± 43.7 vs 58.5 ± 34.6). In conclusion, superovulatory response and embryo yield did not differ between FSH treatments initiated during the first or last follicular wave of a progesterone-based treatment in Santa Ines ewes. Financial support of FAPESP is acknowledged.

scholarly journals Follicle-stimulating hormone to substitute equine chorionic gonadotropin in the synchronization of ovulation in Santa Inês ewes

2012 ◽  
Vol 41 (3) ◽  
pp. 603-606 ◽  
Author(s):  
Bianor Matias Cardoso Neto ◽  
Larissa Pires Barbosa ◽  
Cristiane da Silva Aguiar ◽  
Rosiléia Silva Souza ◽  
Ana Lúcia Almeida Santana ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Follicle Stimulating Hormone ◽  
Santa Inês ◽  
Equine Chorionic Gonadotropin ◽  
Stimulating Hormone

scholarly journals 213 USE OF ACTIVE CASPASE 3 AND TUNEL ASSAYS TO ESTIMATE EMBRYONIC QUALITY IN IN VIVO SANTA INES EWE EMBRYOS

2014 ◽  
Vol 26 (1) ◽  
pp. 220 ◽  
Author(s):  
M. E. F. Oliveira ◽  
C. S. Oliveira ◽  
M. R. Lima ◽  
F. F. P. C. Barros ◽  
A. P. Perini ◽  
...  
Keyword(s):  
Breeding Season ◽  
Caspase 3 ◽  
Transition Period ◽  
Pearson Correlation ◽  
Apoptotic Cells ◽  
Nonbreeding Season ◽  
Follicular Wave ◽  
Santa Inês ◽  
Active Caspase

This study was designed to quantify the percentage of apoptotic cells using active caspase 3 and TUNEL assays, in order to estimate the quality of ovine embryos produced in vivo. For that, 60 Santa Ines ewes (n = 10 per group) were submitted to superovulation with FSH treatment started near the different follicular wave emergence of the protocols (G-1 or G-2), during breeding season, transition, and nonbreeding season. Follicular wave emergence days were defined in a previous study that evaluated the follicular dynamic in oestrus synchronization treatments (Oliveira et al. 2011 Acta Sci. Vet. 38, 361). On Day 0, all ewes received a P4 device (CIDR®) and 37.5 μg of D-cloprostenol. The P4 device was replaced by a new one on Day 7 just for G-2 in the transition period. The superovulation treatment started on Day 4, 4, and 6 of protocol for G-1 and on Day 10, 10, and 11 for G-2 in nonbreeding, transition, and breeding season, respectively. The FSH treatment consisted of eight injections of pFSH administrated twice a day in descending order (40, 30, 20, and 10 mg of pFSH). The P4 device was removed two days after beginning of FSH treatment. At these times, all ewes received another injection of 37.5 μg of D-cloprostenol and a dose of 200 IU of eCG. Ewes were mated with a fertile ram for 4 days after P4 device removal. Embryo recoveries were carried out by laparotomy, 7 days after CIDR withdrawal. Embryos were morphologically classified. Grade I to III morulas and blastocysts were considered viable. A representative sample of each treatment was fixed and stained by active caspase 3 and TUNEL assays to assess the apoptotic cells percentage. Data were analysed by GLIMMIX using SAS comparing mean values (±s.e.m.) between groups at each season (P = 0.05). Pearson correlation was estimated between active caspase 3 and TUNEL assays. No effect was detected between treatments in each season on the number of viable embryos (3.2 ± 0.8 v. 1.8 ± 0.8, 3.9 ± 1.9 v. 5.7 ± 1.4, and 3.8 ± 1.5 v. 3.4 ± 0.8 for G-1 v. G-2 in nonbreeding, transition and breeding season, respectively). The treatment G-2 increased (P < 0.05) apoptotic cells percentage in nonbreeding season group, assessed by active caspase 3 (G-1: 3.1 ± 1.6% and G-2: 12.8 ± 4.3%) and TUNEL (G-1: 1.6 ± 0.5% and G-2: 11.1 ± 3.5%) assays. The apoptotic cells percentage remained unaltered for Transition and Breeding season groups, assessed by either active caspase 3 (G-1: 6.0 ± 0.9% and 5.6 ± 1.5%; G-2: 5.6 ± 1.1% and 5.1 ± 0.5%) and by TUNEL (G-1: 7.5 ± 1.3% and 5.2 ± 1.0%; G-2: 5.0 ± 0.9% and 6.4 ± 1.1%). The Pearson correlation between active caspase 3 and TUNEL tests was r2 = 0.436 (P < 0.0001). In conclusion, the active caspase 3 and TUNEL assays presented similar results for apoptosis level assessment in Santa Ines ewes in vivo produced embryos, and both assays were considered appropriate for this purpose. The increased apoptosis levels detected in the G-2 nonbreeding season group suggest that this treatment is harmful for Santa Ines ewe embryos. Financial support was provided by FAPESP and FUNDUNESP.

268 BOVINE AND EQUINE RECOMBINANT FOLLICLE-STIMULATING HORMONE PRODUCED IN TWO HETEROLOGOUS HOSTS: ESCHERICHIA COLI AND TRYPANOSOMA BRUCEI

2006 ◽  
Vol 18 (2) ◽  
pp. 241
Author(s):  
M. Wilson ◽  
J. Morris ◽  
J. Gibbons
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Trypanosoma Brucei ◽  
Embryo Transfer ◽  
Follicle Stimulating Hormone ◽  
Production Costs ◽  
Proper Function ◽  
Recombinant Fsh ◽  
Follicular Wave ◽  
Stimulating Hormone

Follicle-stimulating hormone (FSH) is a heterodimeric glycoprotein hormone consisting of � and � subunits produced by different genes. Following release from the anterior pituitary, FSH is responsible for development of ovarian follicles from the time of emergence of a follicular wave until divergence of the dominant follicle. Treatment of cattle and horses with recombinant FSH would be valuable for induction of superovulation in an effort to harvest multiple embryos for use in embryo transfer. Glycosylation of the subunits may be essential for proper function and receptor recognition, thereby complicating the production of recombinant FSH. Therefore, a eukaryotic, rather than a prokaryotic, host would be most beneficial for generation of recombinant protein. To explore recombinant protein synthesis, we utilized the ancient protozoan Trypanosoma brucei. Perhaps best known for its parasitic life style, T. brucei has been the focus of extensive biochemical and genetic research, with the nonpathogenic insect stage of the parasite serving as a model eukaryote for the study of many fundamental eukaryotic pathways, including glycosyl phosphatidylinositol (GPI) anchor biosynthesis and RNA editing. The ease of molecular manipulation of this protozoan, and the organism's robust glycosylation machinery, has led us to explore the possibility of using the non-pathogenic life stage for recombinant protein production. The objective of this study was to produce recombinant bovine and equine FSH in both prokaryotic and eukaryotic expression systems. Protein has been produced in Escherichia coli using the pQE30 expression system. Subunit genes were ligated into pQE30 and expressed in M15 E. coli cells. These cells were found to produce detectable amounts of the individual subunit protein after a three hour induction. To express recombinant protein in T. brucei, subunit genes were cloned into pLEW2T7(6His), a stably integrated vector designed to allow inducible expression of proteins fused to a C-terminal six-histidine tag. Clones were subsequently transformed into T. brucei and are awaiting protein expression. All protein purification will be performed via nickel affinity chromatography and protein concentration will be measured using radioimmunoassay. Bioactivity will be analyzed using a rat ovarian weight assay prior to use in cattle and horses. The purified protein will be administered as intramuscular injections twice daily for four days beginning on the day of follicular wave emergence. Producing recombinant FSH will be useful for the embryo transfer and food safety industries by increasing consistency of biologic activity, decreasing production costs, and, perhaps most importantly, decreasing the opportunity for zoonotic transmission of prions such as bovine spongiform encephalopathy.

Ovarian follicular dynamics and concentrations of oestradiol-17 beta, progesterone, luteinizing hormone and follicle stimulating hormone during the periovulatory phase of the oestrous cycle in the cow

1991 ◽  
Vol 3 (5) ◽  
pp. 529 ◽  
Author(s):  
H Kaneko ◽  
T Terada ◽  
K Taya ◽  
G Watanabe ◽  
S Sasamoto ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Follicular Phase ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Follicular Wave ◽  
Ovulatory Follicle ◽  
Follicular Dynamics ◽  
Stimulating Hormone

Changes in the plasma concentration of oestradiol-17 beta, progesterone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) were characterized during the transition from the luteal to the follicular phase, the periovulatory period and the early luteal phase in five cycling cows. The pattern of growth and the regression of follicles and corpora lutea in the ovary of the same animals were also assessed by daily ultrasonographic examinations. Two waves of follicular growth (ovulatory and non-ovulatory) occurred in all animals. The ovulatory follicular wave started from 4 days before the preovulatory surges of LH and FSH and the wave of next growth of a dominant follicle (non-ovulatory follicle) started within one day after ovulation. Changes in plasma levels of oestradiol-17 beta correlated well with the growth of both ovulatory and non-ovulatory dominant follicles. Suppression of FSH concentration during the follicular phase was inversely related to the increase in plasma concentration of oestradiol-17 beta. These results suggest that, in the cow, ovulatory dominant follicles suppress FSH secretion by increasing the concentration of oestradiol-17 beta (and probably also inhibin) during the follicular phase.

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