155 EFFECT OF VITRIFICATION OF MORULAE ON PREGNANCY RATE IN GOAT

2015 ◽  
Vol 27 (1) ◽  
pp. 168
Author(s):  
M. M. Toishibekov ◽  
G. A. Valieva ◽  
S. M. Askarov
Keyword(s):  
Liquid Nitrogen ◽  
Statistical Difference ◽  
Developmental Competence ◽  
Alternative Methods ◽  
Heat Transmission ◽  
Group V ◽  
Bovine Semen ◽  
Student’S T ◽  
Student’S T Test ◽  
Fresh Embryos

This work evaluated alternative methods for goat morulae cryopreservation by using the High Security Vitrification Kit (Cryobiosystem): vitrification (V) and super-cooling ultra-rapid vitrification (SCURV). Vitrification was applied according to the method described by Vajta et al. (1998). Both treatments used a vitrification solution (VS) containing 20% ethylene glycol (EG), 20% dimethylsulfoxide (Me2SO), 0.5 mol L–1 sucrose in DPBS with 10% BSA. In our experiment we used the Vit-Master™ (MTG, Germany). Super-cooled liquid nitrogen (LN) facilitates heat transmission between LN and the cryosolution interface suggested to be beneficial for bovine semen and blastocyst cryoconservation. By surgical flushing of 30 super-stimulated goats, 137 transferable morulae were harvested; 41 morulae were transferred fresh to synchronized recipients (control) and the others were cryopreserved by V (n = 47) or SCURV (n = 49), respectively thawed, and transferred to recipients. Embryos were vitrified using the HSV Kit. They were first incubated in 50% VS for 2 min and then transferred for 30 s into 100% VS followed by vitrification (group V). Accordingly, morula of SCURV group were exposed to 50% VS for 2 min and to 100% VS for 30 s at 37°C. Thereafter, embryos were transferred into the VIT-Master for freezing with liquid nitrogen using negative pressure. Thawing of vitrified embryos was accomplished by placing the vitrified embryos in solutions of 0.25 M sucrose for 2 min and 0.125 M sucrose for 3 min, respectively. After thawing only survived embryos were transferred. Statistical analyses were performed with Student's t-test. After transfer of fresh or frozen-thawed embryos of V and SCURV groups, 25, 17, and 19 kids were born. No statistical difference was found for the percentage of viability of thawed embryos after vitrification (36.2 ± 4.4%), and SCURV methods (38.7 ± 6.5%). The survival of fresh embryos, however, was significantly higher (60.9 ± 5.3%). Differences were statistically significant (P < 0.05). Importantly, our data suggest that the SCURV method can be used for cryopresevation of goat morulae. Nevertheless, further work regarding the developmental competence of embryos cryopreserved with the SCURV method is needed.

44 SUCCESSFUL KIDDING AFTER ULTRARAPID VITRIFICATION OF GOAT EMBRYOS

2017 ◽  
Vol 29 (1) ◽  
pp. 129
Author(s):  
Y. Toishibekov ◽  
M. Yermekova
Keyword(s):  
Survival Rate ◽  
Liquid Nitrogen ◽  
Statistical Difference ◽  
Developmental Competence ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Heat Transmission ◽  
Bovine Semen ◽  
Fresh Frozen ◽  
Fresh Embryos

This work evaluated methods for goat morulae cryopreservation by using cryoloop: vitrification (V) and super-cooling ultra-rapid vitrification (SCURV). The vitrification method was applied according to the method described by Vajta et al. (1998 Mol. Reprod. Dev. 51, 53–58). Both treatments used a vitrification solution [VS: 20% (3.6 mol L−1), ethylene glycol (EG), 20% (2.4 mol L−1) dimethylsulfoxide (Me2SO)] 0.5 mol L−1 sucrose in DPBS with 10% BSA in both methods. In our experiment, we used the Vit-Master™ (MTG, Bruckberg, Germany). The super-cooled LN facilitates heat transmission between LN and the cryosolution interface and this is efficient for bovine semen and blastocyst cryoconservation (Arav et al. 2002 Mol. Cell. Endocrinol. 187, 77–81). By surgical flushing 25 super stimulated goats, 127 transferable morulae were harvested; 39 morulae were transferred fresh to synchronized recipients (control) and the others were cryopreserved by V (n = 46) or SCURV (n = 42), respectively thawed or warmed, and transferred to recipients. Embryos were vitrified using the cryoloop. They were first incubated in 50% VS for 2 min and then transferred for 30 s into 100% VS. Each embryo was loaded by cryoloop, which was immediately submerged into and stored in liquid nitrogen. Warming was done by placing the narrow end of the cryoloop into DPBS + 0.25 M sucrose for 5 min. Embryos were then transferred into DPBS + 0.125 M sucrose for 3 min and finally to DPBS until transfer. The SCURV morulae were then exposed to 50% and 100% VS at 37°C for 2 min and 30 s, respectively. Embryos after saturation in VS were transferred by cryoloop and using negative pressure of liquid nitrogen in the chamber for freezing with the VIT-Master. Thawing vitrify embryos was accomplished by placing the vitrified embryos in solutions of sucrose 0.25 M and 0.125 M with 2- and 3-min exposures accordingly. After thawing, embryos were transferred. Statistical analysis was done using Student’s test. The kidding rate following transfer of fresh, frozen-thawed vitrification, and SCURV methods were 22, 16, and 16 kids, respectively. No statistical difference was found for the percentage of does kidding following transfer thawed after vitrification (34.7 ± 4.5%a), and SCURV methods (38.1 ± 5.9%b). The survival rate following transfer of fresh embryos (56.4 ± 4.9c) was higher and in line with previous findings using VS. Differences were statistically significant (ac, bc P < 0.05). Importantly, our data suggested that the SCURV method can be used for cryopreservation of goat morulae and has similar success to the vitrification method. While further work on the developmental competence of embryos cryopreserved with the SCURV method is needed, we hypothesise that SCURV, with a faster freeze rate and potentially a lower level of cryoprotectants, may be able to minimize ice crystal formation; SCURV should be further evaluated as a routine mechanism for cryopreserving goat embryos.

120 SUCCESSFUL LAMBING AFTER SUPER-COOLING ULTRARAPID VITRIFICATION SHEEP MORULAE

2010 ◽  
Vol 22 (1) ◽  
pp. 218
Author(s):  
Y. M. Toishibekov ◽  
H. D. Blackburn
Keyword(s):  
Ethylene Glycol ◽  
Liquid Nitrogen ◽  
Toxic Elements ◽  
Developmental Competence ◽  
Alternative Methods ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Heat Transmission ◽  
Bovine Semen ◽  
Loop Volume

The aim of this work was to establish alternative methods for sheep morulae cryopreservation by using vitrification by open pulled straw (OPS) methods and super-cooling ultra-rapid vitrification (SCURV). Both treatments used a vitrification solution (VS) of 20% (3.6 mol L-1) ethylene glycol (EG), 20% (2.4 mol L-1) dimethylsulfoxide (DMSO), 0.5 mol L-1 sucrose in DPBS with 10% BSA in both methods. In our experiment we used the Vit-Master™ (MTG, Germany). The super-cooled LN facilitates heat transmission between LN and the cryosolution interface, and this is efficient for bovine semen and blastocyst cryoconservation (Arav et al. 2002). By surgical flushing of 24 super stimulated ewes 121 transferrable morulae were harvested; 30 morulae were transferred fresh to synchronised recipients and the others were cryopreserved by OPS (n = 49) or SCURV (n = 42), respectively thawed or warmed, and transferred to recipients. Embryos were vitrified using the OPS method. They were first incubated in 50% VS for 2 min and then transferred for 30 s into 100% VS. Each embryo was loaded by touching a 1-μL drop with the straw, which was immediately submerged into and stored in liquid nitrogen. Warming was done by placing the narrow end of the straw into DPBS + 0.25M sucrose for 5 min. Embryos were then transferred into DPBS + 0.125 M sucrose for 3 min and finally to DPBS until transfer. The SCURV morulae were then exposed to 50 and 100% VS at 37°C for 2 min and 30 s, respectively. Embryos after saturation VS have been transferred by on a surface of a nylon loop (volume 20 μL, diameter 0.5 mm) and using negative pressure temperature of liquid nitrogen in the chamber for freezing with the VIT-Master. Thawing vitrified embryos was accomplished by placing the vitrified embryos in solutions of sucrose 0.25 and 0.125 with expositions of 2 and 3 min, accordingly. After embryos were thawed, only good quality embryos were transferred. Importantly, our data suggest that by using the SCURV method, the toxic elements contained in the cryopreservation solution can be reduced while maintaining a similar ability to produce viable morulae for implantation as the OPS method. Although further work on the developmental competence of embryos cryopreserved with the SCURV method are needed, these data suggest that the faster freeze rate and lower levels of cryoprotectants of SCURV are able to minimize ice crystal formation and should be further evaluated as a routine mechanism for cryopreserving sheep morulae. Table 1.Effect vitrification and ultra-rapid super-cooling vitrification on the viability and lambing of sheep morulae

45 EFFECT OF VITRIFICATION ON KIDDING OF CAPRINE EMBRYOS

2014 ◽  
Vol 26 (1) ◽  
pp. 136
Author(s):  
M. M. Toishibekov ◽  
H. Blackburn ◽  
G. A. Valieva ◽  
S. M. Askarov ◽  
B. B. Molzhigitov
Keyword(s):  
Survival Rate ◽  
Ethylene Glycol ◽  
Statistical Difference ◽  
Developmental Competence ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Heat Transmission ◽  
Bovine Semen ◽  
Fresh Frozen ◽  
Fresh Embryos

This work evaluated different methods: vitrification (V) and super-cooling ultra-rapid vitrification (SCURV). The goat morulae were cryopreserved into the High Security Vitrification (HSV) Kit (Cryo Bio System, Paris, France). The vitrification method was applied according to the method described by Vajta et al. (1998). Both treatments used a vitrification solution [VS; 20% (3.6 mol L–1) ethylene glycol (EG), 20% (2.4 mol L–1) dimethyl sulfoxide (Me2SO), and 0.5 mol L–1 of sucrose in Dulbecco's PBS (DPBS) with 10% BSA in both methods]. In our experiment, we used the Vit-Master™ apparatus (MTG GmbH, Bruckberg, Germany). The supercooled LN facilitates heat transmission between LN and the cryosolution interface and this is efficient for bovine semen and blastocyst cryoconservation (Arav et al. 2002). By surgical flushing of 30 superstimulated (1200 IU of Folligon, Intervet International, Boxmeer, the Netherlands) goats, 137 transferable morulae were harvested; 41 morulae were transferred fresh to synchronized recipients (control) and the others were cryopreserved by V (n = 47) or SCURV (n = 49), respectively thawed or warmed, and transferred to recipients. Embryos were vitrified using the HSV Kit. They were first incubated in 50% VS for 2 min and then transferred for 30 s into 100% VS. Each embryo was loaded by HSV Kit, which was immediately submerged into and stored in LN. Warming was done by placing the narrow end of the straw into DPBS + 0.25 M sucrose for 5 min. Embryos were then transferred into DPBS + 0.125 M sucrose for 3 min and finally to DPBS until transfer. The SCURV morulae were then exposed to 50 and 100% VS at 37°C for 2 min and 30 s, respectively. Embryos after saturation in VS were transferred by HSV Kit and using negative pressure of LN in the chamber for freezing with the VIT-Master. Thawing vitrified embryos was accomplished by placing the vitrified embryos in solutions of sucrose 0.25 and 0.125 M, with exposures of 2 and 3 min, accordingly. After thawing embryos, only good-quality embryos were transferred. The kidding rate following transfer of fresh, frozen-thawed vitrification, and SCURV methods were 25, 17, and 19 kids, respectively. No statistical difference was found for the percentage of does kidding following transfer of thawed embryos after vitrification (36.2 ± 4.4%a) and SCURV methods (38.7 ± 6.5%b). The survival rate following transfer of fresh embryos (60.9 ± 5.3c) was higher and in line with previous findings using VS. Differences were statistically significant (ac, bc: P < 0.05). Importantly, our data suggest that the SCURV method can be used for cryopreservation of goat morulae as the vitrification method. Although further work on the developmental competence of embryos cryopreserved with the SCURV method are needed, these data suggest that with SCURV, a faster freeze rate and lower level of cryoprotectants is able to minimize ice crystal formation and should be further evaluated as a routine mechanism for cryopreserving goat embryos.

57 THE EFFECT OF VITRIFICATION FOR SHEEP EMBRYO VIABILITY

2015 ◽  
Vol 27 (1) ◽  
pp. 121
Author(s):  
G. A. Valieva ◽  
M. M. Toishibekov ◽  
S. M. Askarov ◽  
B. B. Molzhigitov
Keyword(s):  
Developmental Competence ◽  
Embryo Cryopreservation ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Embryo Viability ◽  
Fresh Frozen ◽  
Loop Volume ◽  
Student’S T ◽  
Phosphate Buffered Saline ◽  
Student’S T Test

This work evaluated different methods for sheep embryo cryopreservation by vitrification (V) and super-cooling ultra-rapid vitrification (SCURV). The vitrification method was applied according to the method described by Vajta et al. Both treatments used a vitrification solution (VS) containing 20% ethylene glycol, 20% dimethylsulfoxide (Me2SO), 0.5 mol L–1 sucrose in Dulbecco's phosphate buffered saline (DPBS) with 10% BSA. The super-cooled LN facilitates heat transmission between LN and the cryosolution interface, and this is efficient for bovine semen and blastocyst cryoconservation (Arav et al. 2002). By surgical flushing 25 super-stimulated ewes, 109 transferable morulae were harvested; 35 morulae were transferred fresh to synchronized recipients (control) and the others were cryopreserved by V (n = 36) or SCURV (n = 38), respectively, thawed or warmed, and transferred to recipients. Embryos were vitrified using the HSV Kit. They were first incubated in 50% VS for 2 min and then transferred for 30 s into 100% VS. Each embryo was loaded by HSV Kit, which was immediately submerged into and stored in LN. Warming was done by placing the narrow end of the straw into DPBS + 0.25 M sucrose for 5 min. Embryos were then transferred into DPBS + 0.125 M sucrose for 3 min and finally to DPBS until transfer. The SCURV morulae were then exposed to 50 and 100% VS at 37°C for 2 min and 30 s, respectively. Embryos after saturation in VS were transferred on a surface of a nylon loop (volume 20 μL, diameter 0.5 mm) and using negative pressure of LN in the chamber for freezing with the VIT-Master. Thawing vitrified embryos was accomplished by placing the vitrified embryos in solutions of sucrose 0.25 M and 0.125 M with expositions 2 and 3 min accordingly. After thawing embryos, only good-quality embryos were transferred. Statistical analyses were performed with Student's t-test. The lambing rate following transfer of fresh, frozen-thawed vitrification and SCURV methods were 18, 12, 14 lambs accordingly. No statistical difference was found for the percentage of does lambing following transfer thawed after vitrification (33.4 ± 5.2a%) and SCURV methods (36.8 ± 6.3b%). The survival rate following transfer of fresh embryos (51.4 ± 4.8c) was higher and in line with previous findings using VS. Differences were statistically significant (ac,bc P < 0.05). Importantly, our data suggest that the HSV Kit can be used to produce viable morulae for implantation as the SCURV, and to as vitrification method. Although further work on the developmental competence of embryos cryopreserved with the SCURV method are needed, these data suggest that with SCURV a faster freeze rate and lower level of cryoprotectants is able to minimize ice crystal formation and should be further evaluated as a routine mechanism for cryopreserving sheep embryos.

scholarly journals THE INFLUENCE OF STREET TREES ON URBAN MICROCLIMATE

FLORESTA ◽  
2020 ◽  
Vol 50 (3) ◽  
pp. 1486
Author(s):  
Angeline Martini ◽  
Daniela Biondi ◽  
Antonio Carlos Batista
Keyword(s):  
Wind Speed ◽  
Relative Humidity ◽  
Statistical Difference ◽  
Urban Trees ◽  
Street Trees ◽  
Four Seasons ◽  
Three Samples ◽  
Collection Period ◽  
Student’S T ◽  
Student’S T Test

The microclimatic improvement is often cited as one of the benefits generated by urban trees, however, there are few studies which quantitatively address this effect. The aim of this paper was to compare the microclimate of streets with and without trees. Therefore, three samples containing a stretch of street with and without trees were selected, with the street with trees being composed of different species. The temperature, relative humidity and wind speed data were measured by automatic Kestrel® meteorological ministations installed on the street with trees and another in the street without trees. The data collection period was from 9 AM to 3 PM, and the monitoring interval was every 1 minute during all four seasons starting in the winter of 2011. The comparative analyses were done using the Student’s t-test (99%). The results indicated that the air temperature in a street with trees was on average 1.7 °C lower than a street without trees, the relative humidity was 6.9% higher and the wind speed was 0.04 m/s lower.  There was no statistical difference for temperature between the street with “ipê” in the winter and spring. There was no difference for wind speed in the summer between the street with other trees and the street with “ipê” and “tipuana”. It was concluded that street trees provide a pleasant microclimate, and this influence was statistically significant.

scholarly journals Root Dentine Strain Generation during Root Canal Instrumentation with Rotation and Reciprocation Motions

2020 ◽  
Vol 10 (2) ◽  
pp. 66-70
Author(s):  
Bnar O. Hama faraj ◽  
Raid F. Salman
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Strain Measurement ◽  
Statistical Difference ◽  
Strain Gauges ◽  
Student’S T ◽  
Rotation Motion ◽  
Root Canal Instrumentation ◽  
Dentine Surface ◽  
Student’S T Test

The aim of the study is to measure and compare strain generated on root dentine surface by two different instrumentation motions. Thirty extracted single canal premolars were used in this study which randomly was divided into two groups, one group was prepared with ProTaper NEXT X2 file in rotation motion, and the second group was prepared by WaveOne GOLD primary file in reciprocation motion, the canals were irrigated during canal preparation by 2 ml of sodium hypochlorite and 17% EDTA gel was used. Strain measurement was performed by strain gauges and a digital strain output reader, data were recorded every 5 s for 50 s. The results of this study have shown that WaveOne GOLD had higher mean 0.7308±0.41106 than ProTaper next 0.5385±0.39904 which means WaveOne GOLD generates greater strain than ProTaper NEXT. However, according to Student’s t-test between two groups there is no statistical difference between them. Based on the results of this study, there was no difference between the two motions; both motions generated strain on root dentine surface.

scholarly journals Comments on “Short-Term Precipitation and Temperature Trends along an Elevation Gradient in Northeastern Puerto Rico”

2017 ◽  
Vol 21 (6) ◽  
pp. 1-4 ◽  
Author(s):  
Angel R. Torres-Valcárcel ◽  
Cesar Gonzalez-Avilés
Keyword(s):  
Elevation Gradient ◽  
Data Distribution ◽  
Climate Science ◽  
Alternative Methods ◽  
Short Term ◽  
Gaussian Distributions ◽  
Temperature Trends ◽  
Student’S T ◽  
Selection Of ◽  
Student’S T Test

Abstract The selection of statistical methods to evaluate data depends on study questions and characteristics of available data. In climate science, some methods are more popularly used than others; however, the use of applicable alternative methods does not invalidate study findings. Regardless of limitations, some methods like Pearson ordinary correlation are widely used in all sciences including climate and by scientists at government agencies like NOAA and the USGS. In addition, the use of the robust Student’s t test is valid for near-Gaussian distributions with high sample numbers, since it is resistant to data distribution inconsistencies. We wish to put in context the citation about our article and clarify the methods and justification for using them and to educate readers about the use of some conventional statistical tools and tests.

scholarly journals Pathogenicity and virulence of entomopathogenic nematodes against Dichelops melacanthus Dallas (Hemiptera: Pentatomidae)

2019 ◽  
Vol 40 (4) ◽  
pp. 1417
Author(s):  
Bruna Aparecida Guide ◽  
Viviane Sandra Alves ◽  
Thiago Augusto Paes Fernandes ◽  
Matheus Corseti Marcomini ◽  
Ana Maria Meneghin ◽  
...  
Keyword(s):  
Entomopathogenic Nematodes ◽  
Alternative Methods ◽  
Steinernema Feltiae ◽  
Stink Bug ◽  
Greenhouse Test ◽  
Randomized Design ◽  
Selection Test ◽  
Completely Randomized Design ◽  
Student’S T ◽  
Student’S T Test

Infestations of Dichelops melacanthus (Dallas; Hemiptera: Pentatomidae) in corn and wheat in Brazil, and the subsequent damage, have increased in recent years, mainly owing to this insect’s ability to survive the off-season. The control of this insect is mainly carried out with chemical insecticides, but the development of alternative methods, such as biological control, can contribute to a more sustainable management. Thus, the objective of this study was to evaluate the potential of entomopathogenic nematodes (EPNs) for the control of D. melacanthus. A selection test was performed with 15 isolates of genera Steinernema and Heterorhabditis regarding their pathogenicity and virulence on adults of D. melacanthus. Concentration (10, 20, 40, 50, and 100 infective juveniles (IJs)/cm2) and greenhouse tests were carried out only with the Steinernema feltiae isolate (IBCB-n 47). All experiments were conducted in a completely randomized design. The selection test data were submitted to the Scott-Knott averages test (P ? 0.05), and those from the greenhouse test to the Student's t-test. The results of the concentration assay were subjected to regression analysis. All isolates showed pathogenicity and virulence in adults of D. melacanthus. The isolates GL (Heterorhabditis amazonensis), IBCB-n27 (Steinernema sp.), and RSC05 (H. amazonensis) were the most virulent (80.0, 82.0, and 88.0% mortality, respectively). The higher concentrations of S. feltiae (50 and 100 IJs/cm²) were responsible for the highest mortality rates of green belly stink bug (88.0 and 86.0%, respectively). In the greenhouse test, S. feltiae caused higher mortality (38%) than the control.

Serosal and cutaneous recordings of gastric myoelectrical activity in patients with gastroparesis

1994 ◽  
Vol 266 (1) ◽  
pp. G90-G98 ◽  
Author(s):  
J. D. Chen ◽  
B. D. Schirmer ◽  
R. W. McCallum
Keyword(s):  
Electrical Stimulation ◽  
Slow Wave ◽  
Test Meal ◽  
Frequency Change ◽  
Fasting State ◽  
Gastric Myoelectrical Activity ◽  
Myoelectrical Activity ◽  
Gastric Slow Wave ◽  
Student’S T ◽  
Student’S T Test

The aims of this study were to 1) investigate gastric myoelectrical activity in patients with gastroparesis, 2) validate the cutaneous electrogastrogram (EGG) in tracking the frequency change of the gastric slow wave, and 3) investigate the effect of electrical stimulation on gastric myoelectrical activity. Gastric myoelectrical activity was recorded in 12 patients with documented gastroparesis using serosal electrodes for > 200 min in each subject. All recordings were made at least 4 days after surgery. Each session consisted of a 30-min recording in the fasting state and a 30-min recording after a test meal. The test meal (liquid or mixed) was selected according to patient's tolerance. Electrical stimulation was performed in three subjects via the serosal electrodes at a frequency of 3 cycles/min. Gastric myoelectrical activity was recorded using serosal electrodes in each session. The serosal recording showed slow waves of 2.5 to 4.0 cycles/min in all 12 subjects. Absence of spikes was noted in 11 of the 12 subjects. The simultaneous serosal and cutaneous recording of gastric myoelectrical activity showed that the frequency of the EGG was exactly the same as that of the serosal recording. Liquid meals resulted in a significant decrease in slow-wave frequency (Student's t test, P = 0.006), and the EGG accurately reflected this change. Electrical stimulation had no effect on the frequency of the gastric slow wave and did not induce spikes.(ABSTRACT TRUNCATED AT 250 WORDS)

The Effect of Changes in Dialysate Volume on Glucose and Urea Equilibration

1994 ◽  
Vol 14 (3) ◽  
pp. 236-239 ◽  
Author(s):  
Edward C. Kohaut ◽  
F. Bryson Waldo ◽  
Mark R. Benfield
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Prospective Study ◽  
Body Surface ◽  
Patient Population ◽  
Different Ages ◽  
Student’S T ◽  
A Prospective Study ◽  
Dialysate Volume ◽  
Student’S T Test

Objectives To determine the effect of changing dialysate volume on urea and glucoseequilibration curves and to determine, if dialysate volume is prescribed on the basis of body surface area, whether equilibration curves will be consistent in patients of different sizes and ages. Design A prospective study wherein children with acute or chronic renal failure had peritoneal equilibration studies done with dwell volumes of 30 mL/kg, 40 mL/kg, and 1200 mL/m2. Patient Population Twenty-two children: 7 under 3 years of age; 8 between 3 and 10 years of age; 7 older than 10 years of age. Statistics Student's t-test. Results Urea and glucose equilibrated rapidly at dwell volumes of 30 mL/kg, slower at dwell volumes of 40 mL/kg, and slowest at dwell volumes of 1200 mL/m2. Equilibration curves were similar in children of different ages when dialysate volumes of 1200 mL/m2 were infused. Conclusion Dialysate volumes of 1200 mL/m2 should be used when equilibration studies are being done to compare individuals of different ages and sizes.

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