2 TRANSCRIPTOME ANALYSIS OF GRANULOSA CELLS FROM GROWING DOMINANT FOLLICLE REVEALS AGE-ASSOCIATED CHANGES AT THE TIME OF FOLLICLE SELECTION IN AGED BEEF CATTLE

2013 ◽  
Vol 25 (1) ◽  
pp. 148 ◽  
Author(s):  
M. I. R. Khan ◽  
F. C. F. Dias ◽  
M. A. Sirard ◽  
G. P. Adams ◽  
J. Singh
Keyword(s):  
Cytochrome P450 ◽  
Granulosa Cells ◽  
Prostaglandin F2α ◽  
Differentially Expressed ◽  
Nuclear Antigen ◽  
Dominant Follicle ◽  
Age Related ◽  
Junction Protein ◽  
Cytochrome P450 Family ◽  
Follicle Selection

Growing dominant follicles at the time of selection from aged (n = 3; 15 ± 1.5 years) and young (n = 3; 6 ± 1.1 years) Hereford cows were compared using bovine-specific microarrays containing 40 000 targets. The objective of the study was to determine age-associated changes in transcriptome of granulosa cells at the time of dominant follicle selection. Cows were given prostaglandin F2α to cause ovulation (Day 0) and granulosa cells from dominant follicles were collected on Day 3 either by ultrasound-guided follicle aspiration or after ovariectomy. The mRNA was extracted, analyzed for quality, converted into antisense RNA, amplified, labelled with red and green florescent dyes, and hybridized with microarrays. Feature intensities were measured using Array-Pro software (Media Cybernetics Inc., Rockville, MD), and differentially expressed genes were obtained using FlexArray 1.6. A total of 169 transcripts were differentially expressed with a fold change of ≥2 (P ≤ 0.05) in aged cows v. young cows. Ingenuity System Pathway (IPA; Ingenuity Systems Inc., Redwood City, CA) analysis of these transcripts revealed that granulosa cells of aged cows exhibit (1) reduced capability to regulate gonadotropins [↓follistatin (FST), ↓inhibin beta A (INHBA), ↓inhibin beta B (INHBB)] and reduced responsiveness to gonadotropin-induced changes in cytoskeleton [↑tropomyosin 2 (TPM2), ↑actin gamma 2 (ACTG2), ↓tubulin beta] and extracellular matrix [↓tumor necrosis factor alpha-induced protein 6 (TNFAIP6), ↓versican (VCAN)], (2) inefficiency in processing lipids [↓low-density lipoprotein receptor (LDLR), ↓stearoyl-coenzyme A desaturase (SCD), ↑cluster of differentiation 36 (CD36), ↓sterol-C4-methyl oxidase-like (SC4MOL)] and synthesizing steroids [↓cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), ↓cytochrome P450, family 51, subfamily A, polypeptide 1 (CYP51A1)], (3) decreased proliferation [↓proliferating cell nuclear antigen (PCNA)] and control of cell cycle check points [↓checkpoint kinase 1 (CHEK1), ↓centromere-associated protein E (CENPE)] and have poor intercellular communication [↓gap junction protein alpha 1 (GJA1)], and (4) higher expression of oxidative stress responsive genes [↑vanin-1 (VNN1), ↑vanin-2 (VNN2), and ↑glutathione peroxidase 3 (GPX3)]. A total of 6 transcripts: CYP19A1 (aromatase; P ≤ 0.1), VNN1 (P ≤ 0.05), INHBA (P ≥ 0.1), PCNA (P ≤ 0.001), TPM2 (P ≤ 0.1), and GJA1 (P ≤ 0.05) were selected to validate the microarray results via quantitative real-time PCR. In conclusion, granulosa cells of growing dominant follicles exhibit age-related changes in the transcriptome at the time of selection relative to young cows; changes that may explain follicle-associated loss of oocyte competence in aged cows. Research was supported by the Natural Sciences and Engineering Research Council of Canada (Ottawa, ON, Canada).

Role of A-kinase anchoring protein 95 in the regulation of cytochrome P450 family 19 subfamily A member 1 (CYP19A1) in human ovarian granulosa cells

2018 ◽  
Vol 30 (8) ◽  
pp. 1128 ◽  
Author(s):  
Yu Gu ◽  
Wenbin Xu ◽  
Bole Zhuang ◽  
Wei Fu
Keyword(s):  
Cytochrome P450 ◽  
Granulosa Cells ◽  
Crucial Role ◽  
Polycystic Ovary ◽  
Camp Response Element Binding ◽  
Gene Encoding ◽  
Ovarian Granulosa Cells ◽  
Anchoring Protein ◽  
Element Binding Protein ◽  
Cytochrome P450 Family

Irregular expression of cytochrome P450 family 19 subfamily A member 1 (CYP19A1) is involved in the development of polycystic ovary syndrome (PCOS). Activation of the cAMP/protein kinase A (PKA)/cAMP response element-binding protein (CREB) pathway plays a crucial role in FSH regulation of CYP19A1 in human ovarian granulosa cells. A-Kinase anchor protein 95 (AKAP95) is known to confine PKA to the nucleus. However, it is unclear whether anchoring PKA to the nucleus is essential for the induction of CYP19A1 by FSH in human ovarian granulosa cells. Using the human granulosa cell line KGN and primary cultured human luteinised granulosa cells (hLGCs), we found that knockdown of AKAP8, the gene encoding AKAP95, or inhibition of AKAP95 reduced the amount of PKA anchored in the nucleus and attenuated the phosphorylation of CREB by either FSH or activation of the cAMP/PKA pathway. Moreover, knockdown of AKAP8 or inhibition of AKAP95 also significantly attenuated FSH-induced CYP19A1 expression and oestrogen synthesis. Furthermore, significant decreases in AKAP95 and CYP19A1 were observed in hLGCs obtained from PCOS patients. The results of the present study demonstrate a crucial role for AKAP95 in CYP19A1 expression and oestrogen synthesis in hLGCs, which implies that AKAP95 may be involved in the pathogenesis of PCOS.

scholarly journals Development of an experimental ovarian tumor: immunocytochemical analysis

2002 ◽  
pp. 387-395 ◽  
Author(s):  
E Sorianello ◽  
S Fritz ◽  
C Beyer ◽  
DB Hales ◽  
A Mayerhofer ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Regulatory Protein ◽  
Tumor Development ◽  
Female Rats ◽  
Nuclear Antigen ◽  
Normal Ovary ◽  
Rt Pcr ◽  
Thecal Cells ◽  
Junction Protein ◽  
Steroidogenic Acute Regulatory

OBJECTIVE: The aim of the present work was to study whether immunocytochemical parameters present in the normal ovary were altered after tumor development under high gonadotropin levels. METHODS: Ovarian tumors (luteoma): castrated female rats had an ovary grafted into the spleen; tumors were left to develop for 1, 2, 3 or 7 months. The presence of apoptotic cells (TUNEL method) and the expression of proliferating cell nuclear antigen (PCNA), gap junction protein (Cx43), steroidogenic acute regulatory protein (StAR), aromatase and synaptosome-associated protein of 25 kDa (SNAP-25) were determined by immunocytochemistry. Some of these findings were confirmed by RT-PCR (Cx43, StAR, SNAP-25). Inhibin subunit mRNAs were investigated by Northern blot. RESULTS: PCNA staining of tumors was mainly found in granulosa cells of transforming follicles and was absent from luteinized follicles. A nearly complete absence of apoptosis was observed. Cx43 was mainly found in follicles, while it was very weakly expressed or absent in luteinized follicles. StAR protein expression, indicating active steroidogenesis, was demonstrated only in luteinized follicles and in thecal cells, but was absent from granulosa cells. Aromatase immunoreactivity was very intense in granulosa and also present in luteal cells. Membrane-associated and cytoplasmic SNAP-25 immunostaining was determined in patches of endocrine cells in the follicles, as well as in the luteinized follicles. The expression of mRNAs for Cx43, StAR and SNAP-25 (RT-PCR) and inhibin subunits (Northern blots) were confirmed in 1-, 3- and 7-month-old tumors. CONCLUSIONS: These results indicated that luteoma most likely develop from unruptured follicles by hypertrophy and proliferation of follicular cells. Circulating gonadotropins seem to play a fundamental role in maintaining the expression of proteins typically expressed in normal ovary, while avoiding apoptosis in this tissue.

scholarly journals RNAi-Mediated Silencing of Catalase Gene Promotes Apoptosis and Impairs Proliferation of Bovine Granulosa Cells under Heat Stress

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1060
Author(s):  
Adnan Khan ◽  
Muhammad Zahoor Khan ◽  
Jinhuan Dou ◽  
Saqib Umer ◽  
Huitao Xu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Heat Stress ◽  
Granulosa Cells ◽  
Ovarian Follicle ◽  
Regulatory Protein ◽  
Nuclear Antigen ◽  
Cellular Protection ◽  
Catalase Gene ◽  
Induced Apoptosis ◽  
Cytochrome P450 Family

Heat stress in dairy cattle is recognized to compromise fertility by altering the functions of ovarian follicle-enclosed cells, e.g., oocyte and granulosa cells (GCs). Catalase is an antioxidant enzyme that plays a significant role in cellular protection against oxidative damage by the degradation of hydrogen peroxide to oxygen and water. In this study, the role and mechanism of CAT on the heat stress (HS)-induced apoptosis and altered proliferation of bovine GCs were studied. The catalase gene was knocked-down successfully in bovine GCs at both the transcriptional and translational levels. After a successful knockdown using siRNA, GCs were divided into HS (40 °C + NC and 40 °C + CAT siRNA) and 38 °C + NC (NC) groups. The GCs were then examined for ROS, viability, mitochondrial membrane potential (MMP), cell cycle, and biosynthesis of progesterone (P4) and estrogen (E2) hormones. The results indicated that CAT silencing promoted ROS production and apoptosis by up-regulating the Bcl-2-associated X protein (BAX) and Caspase-3 genes both at the transcriptional and translational levels. Furthermore, the knockdown of CAT markedly disrupted the MMP, impaired the production of P4 and E2, altered the progression of the G1 phase of the cell cycle, and decreased the number of cells in the S phase. This was further verified by the down-regulation of proliferating cell nuclear antigen (PCNA), CyclinB1, steroidogenic acute regulatory protein (STAR), and cytochrome P450 family 11 subfamily A member 1 (Cyp11A1) genes. Our study presented a novel strategy to characterize how CAT can regulate cell proliferation and apoptosis in GCs under HS. We concluded that CAT is a broad regulatory marker in GCs by regulating apoptosis, cellular progression, and simultaneously by vital fluctuations in hormonal signaling. Our findings infer a crucial evidence of how to boost the fertility of heat-stressed cows.

Regulation by 3,5,3ʹ-tri-iodothyronine and FSH of cytochrome P450 family 19 (CYP19) expression in mouse granulosa cells

2018 ◽  
Vol 30 (9) ◽  
pp. 1225 ◽  
Author(s):  
Juan Liu ◽  
Yingying Han ◽  
Ye Tian ◽  
Xuechun Weng ◽  
Xusong Hu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Follicular Development ◽  
Sterol Biosynthesis ◽  
Akt Pathway ◽  
Preantral Follicle ◽  
Follicle Growth ◽  
Cytochrome P450 Family ◽  
Cyp19 Expression

Cytochrome P450 family 19 (CYP19) plays an important role in follicular development, which is regulated by FSH. Although 3,5,3′-tri-iodothyronine (T3) combines with FSH to induce preantral follicle growth and granulosa cell development, the mechanism involved remains unclear. The aim of the present study was to determine the cellular and molecular mechanisms by which thyroid hormone (TH) and FSH regulate CYP19 expression and sterol biosynthesis during preantral follicle growth. Mice were injected subcutaneously (s.c.) with eCG (Equine chorionic gonadotropin). The results showed that eCG increased CYP19 expression in ovarian cells. CYP19 expression in granulosa cells was increased after FSH treatment, and this response was enhanced by T3. Knockdown of CYP19 significantly decreased granulosa cell viability and hormone-stimulated proliferation. In addition, CYP19 knockdown also blocked T3- and FSH-induced oestradiol (E2) synthesis in granulosa cells. Furthermore, activation of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway was required for T3 and FSH regulation of CYP19 expression. In conclusion, the results of the present study indicate that CYP19 is important for T3- and FSH-induced granulosa cell development in the early stages. CYP19 could be a downstream effector of the PI3K/Akt pathway in regulating TH and FSH during follicular development and sterol biosynthesis. The findings suggest that CYP19 is a novel mediator of T3- and FSH-induced follicular development.

Serine protease inhibitor-E2 (SERPINE2) is differentially expressed in granulosa cells of dominant follicle in cattle

2002 ◽  
Vol 64 (2) ◽  
pp. 152-165 ◽  
Author(s):  
Julie Bédard ◽  
Sophie Brûlé ◽  
Christopher A. Price ◽  
David W. Silversides ◽  
Jacques G. Lussier
Keyword(s):  
Protease Inhibitor ◽  
Serine Protease ◽  
Granulosa Cells ◽  
Serine Protease Inhibitor ◽  
Differentially Expressed ◽  
Dominant Follicle

scholarly journals The Roles of the miRNAome and Transcriptome in the Ovine Ovary Reveal Poor Efficiency in Juvenile Superovulation

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 239
Author(s):  
Xiaosheng Zhang ◽  
Chunxiao Dong ◽  
Jing Yang ◽  
Yihai Li ◽  
Jing Feng ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
High Throughput Sequencing ◽  
Hormone Receptors ◽  
Hormone Secretion ◽  
Oocyte Quality ◽  
Differentially Expressed ◽  
Messenger Rnas ◽  
Animal Cloning ◽  
Developmental Capacity ◽  
Cytochrome P450 Family

Juvenile superovulation can provide a wealth of oocyte material for embryo production, animal cloning, and genetic modification research, but embryos derived from juvenile oocytes show poor efficiency in subsequent developmental capacity. In order to reveal the formation mechanism of large numbers of follicles and poor oocyte quality in juvenile ovaries under superovulation treatment, differentially expressed microRNAs (miRNAs) and messenger RNAs (mRNAs) were characterized and investigated in the ovaries of lambs and adult sheep using high-throughput sequencing technology. The majority of differentially expressed miRNAs (337/358) were upregulated in lamb libraries. The expression levels of mRNAs related to hormone receptors (follicle-stimulating hormone receptor, FSHR; luteinizing hormone/choriogonadotropin receptor, LHCGR; estrogen receptor 1, ESR1), steroid hormone secretion (cytochrome P450 family 11 subfamily A member 1, CYP11A1; cytochrome P450 family 17 subfamily A member 1, CYP17A1; cytochrome P450 family 19 subfamily A member 1, CYP19A1), and oocyte quality (pentraxin 3, PTX3; BCL2 apoptosis regulator, BCL2; caspase 3, CASP3) were significantly different between the lamb and adult libraries. The miRNA aor-miR-143, which targets FSHR, was highly and differentially expressed, and PTX3 was predicted to be targeted by oar-miR-485-3p and oar-miR-377-3p in the ovine ovary. A considerable number of miRNAs were predicted to inhibit ESR1 expression in lamb ovaries. In conclusion, oar-miR-143 and FSHR molecules, among others, might regulate follicle formation, and oar-miR-485-3p, oar-miR-377-3p, and PTX3, among others, may be associated with oocyte quality. These identified miRNAs and mRNAs will be beneficial for the prediction of ovarian superovulation potential and screening of oocytes.

scholarly journals Abnormal Expression of the Steroid Hormone Synthesis Pathway Associates with Cattle Ovarian Cysts

2021 ◽  
Author(s):  
Xiaoling Xu ◽  
Jiahua Bai ◽  
Yusheng Qin ◽  
Tao Feng ◽  
Linli Xiao ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Differentially Expressed Genes ◽  
Steroid Hormone ◽  
Differentially Expressed ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovarian Cysts ◽  
Hormone Synthesis ◽  
Follicular Cyst ◽  
Preovulatory Follicles ◽  
Cytochrome P450 Family

Abstract BackgroundWhen a mature follicle fails to ovulate, ovarian cysts can develop and persist in the ovary, interfering with normal ovarian functions. However, the etiology of ovarian cysts remains poorly understood.ResultsBy enzyme-linked immunosorbent assay (ELISA) of the bovine follicle fluid from cystic follicles , we found that cystic follicles were characterized by lower oestradiol (E2), Insulin-like growth factor 1(IGF1), and insulin levels but elevated progesterone (P4) compared with preovulatory follicles (p <0.05). Further gene expression profiling of follicle walls by RNA sequencing (RNA-seq) showed that there are 356 differentially expressed genes between preovulatory follicles and corpus luteum cyst groups, and 582 differentially expressed genes between preovulatory follicles and follicular cyst groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis linked steroid hormone synthesis pathway to the formation of ovarian cysts, and steroidogenic acute regulatory protein (STAR), cytochrome P450, family 11, subfamily A, polypeptide 1 (CYP11A1), hydroxy-delta-5-steroid dehydrogenase, 3-beta- and steroid delta-isomerase 1 (HSD3B1), cytochrome P450, family 17, subfamily A, polypeptide 1 (CYP17A1), and cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1) genes in the steroid hormone synthesis pathway play important roles in this process. ConclusionsAbnormal hormone profiles and expression of steroid hormone synthesis pathway genes related to the formation of ovarian cysts. The findings lay a theoretical foundation for the prevention and treatment of ovarian cysts.

113 EXPRESSION PROFILING OF NONCODING microRNAs IN BOVINE GRANULOSA CELLS OF PREOVULATORY DOMINANT FOLLICLE USING DEEP SEQUENCING

2014 ◽  
Vol 26 (1) ◽  
pp. 170 ◽  
Author(s):  
S. Gebremedhn ◽  
I. Ahmad ◽  
D. Salilew-Wondim ◽  
S. Sahadevan ◽  
M. Hoelker ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Deep Sequencing ◽  
Granulosa Cells ◽  
Molecular Mechanisms ◽  
Oestrous Cycle ◽  
Differentially Expressed ◽  
Follicular Atresia ◽  
Dominant Follicle ◽  
Novel Mirna ◽  
Differentially Expressed Mirna

In cattle, follicles grow in a wave-like pattern, with typically 2 or 3 waves per oestrous cycle. During each wave, one follicle of a cohort becomes dominant (DF), whereas the remaining subordinate follicles (SF) in the cohort undergo atresia. If the endocrine conditions are appropriate (low progesterone), the dominant follicle goes on to ovulate. In order to unravel the molecular mechanisms associated with ovulation and follicular atresia, here we aimed to investigate the expression of short regulatory microRNA (miRNA) in granulosa cells of DF and SF using deep sequencing. For this, Simmental heifers (n = 7) were synchronized according to standard protocols and slaughtered at Day 19 of the oestrous cycle. Follicles were categorized as DF (≥12 mm; n = 5) and SF (≤10 mm; n = 78). Granulosa cells from both follicle groups were used for total RNA (enriched with miRNA) isolation using miRNeasy mini kit (Qiagen GmbH, Hilden, Germany). The RNA concentration and integrity were measured using Nano Drop 8000 spectrophotometer (Nano Drop, Wilmington, DE, USA) and Agilent, 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), respectively. Libraries were constructed by GATC BioTech AG (Konstanz, Germany) and sequenced on Illumina HISEqn 2000. Prediction of both known and novel miRNA was done using miRDeep2 software packages. Quantification of differentially expressed miRNA was done using R software and DESEqn 2 packages. The MiRNA with log2 fold change difference ≥1, P-value ≤0.05, and false discovery rate of ≤0.1 were considered to be significant. Results showed that 318 and 322 known miRNA were detected in DF and SF, respectively. It was shown that 28 miRNA including bta-miR-122, bta-miR-139, and bta-miR-375, and 35 others including bta-miR-138, bta-miR-20b, and bta-miR-33a were uniquely detected in DF and SF, respectively. In addition to the known annotated miRNA, 20 and 24 novel miRNA were detected in DF and SF, respectively. Expression analysis revealed that 65 miRNA were differentially expressed in granulosa cells of SF compared with DF. Thirty miRNA including bta-miR-409a (involved in cell death by targeting genes BCL2l11, BIRC5, and PTEN) and bta-miR-335 (involved in cell proliferation, migration, and differentiation) are up-regulated in SF, whereas 35 miRNA including the miR-183 cluster (bta-mir-183, bta-miR-182, and bta-mir-96) involved in apoptosis inhibition are down-regulated in SF. The pathway analysis of potential target genes of differentially expressed miRNA is found to be involved in pathways, namely Wnt signalling, MAPK signalling, TGF-β signaling, and other pathways related to cell proliferation and apoptosis. In conclusion, the presence of stage-specific miRNA in granulosa cells support the potential role of miRNA in posttranscriptional regulation of genes during follicular development, mainly ovulation and follicular atresia.

230 EXPRESSION OF mRNA ENCODING FGF10 AND COGNATE RECEPTORS (FGFR1B AND FGFR2B) AROUND FOLLICLE DEVIATION IN NELORE HEIFERS

2010 ◽  
Vol 22 (1) ◽  
pp. 273
Author(s):  
A. C. S. Castilho ◽  
M. F. Machado ◽  
D. M. Guerra ◽  
R. Ereno ◽  
C. M. Barros ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Granulosa Cells ◽  
Rna Extraction ◽  
Bos Indicus ◽  
Mrna Abundance ◽  
Morphological Divergence ◽  
Dominant Follicle ◽  
Ultrasound Monitoring ◽  
Follicle Diameter ◽  
Follicle Selection

A member of the FGF7 subfamily, FGF10 acts via FGFR2B and FGFR1B. In bovine antral follicles, FGF-10 was detected in oocytes and theca cells (TC). Levels of mRNA were negatively correlated with intrafollicular concentrations of estradiol, and FGF10 inhibited estradiol production from granulosa cells (GC). In Nellore (Bos indicus), morphological divergence occurs on average 2.5 days after ovulation, when dominant follicle diameter is around 6.0 mm. To gain insight into the involvement of the FGF10 system in the control of follicle selection, we assessed mRNA expression of FGF10 in TC and of FGFR1B and FGFR2B in GC from dominant and subordinate follicles around deviation in Nellore heifers. Thirteen Nellore heifers were hormonally synchronized, and ovulation was detected by ultrasound monitoring every 12 h. Heifers were slaughtered 2 (n =4), 2.5 (n = 5), and 3 (n = 4) days after ovulation. Granulosa cells and TC were separated from the 2 largest follicles and submitted to total RNA extraction. mRNA abundance of CYP19 (aromatase), FGF10, FGFR1B, and FGFR2B was measured by real-time RT-PCR and normalized by the expression of cyclophilin A (CYCA) and GAPDH, for TC and GC, respectively. Dominant and subordinate follicles were considered those expressing the greatest and second-greatest abundance of CYP19 mRNA in GC within each heifer. Effects of follicle status and day on CYP19, FGF10, FGFR2B, and FGFR1B mRNA abundance were tested by ANOVA. On Day 2, FGFR2B mRNA abundance was greater in GC of subordinate follicles compared with dominant follicles (P = 0.006), and that of FGF10 in TC tended to exhibit the same pattern (P = 0.06). Follicle diameter was not different between dominant and subordinate follicles on Day 2 (5.5 ± 0 v. 5.12 ± 0.3 cm). On Day 2.5, FGF10 expression was greater in TC from subordinate follicles (P = 0.01), and FGFR2B expression in GC was no longer different between dominant and subordinate follicles. Follicle diameter was greater in dominant follicles on Day 2.5 (6.7 ± 0.2 v. 5.8 ± 0.3 cm; P = 0.04). On Day 3, no differences were observed between dominant and subordinate follicles for any of the genes assessed. mRNA expression of FGFR1B in GC did not change with follicle status or day. In conclusion, expression of FGF10 and FGFR2B was decreased in dominant follicles around morphological divergence, suggesting their involvement in the mechanisms controlling dominant follicle selection. As FGF10 inhibits estradiol production of GC, we propose that FGF10 and FGFR2B are suppressed in the dominant follicle to allow acquisition of full steroidogenic capacity. This research was supported by FAPESP.

scholarly journals Evidence that fibroblast growth factor 10 plays a role in follicle selection in cattle

2017 ◽  
Vol 29 (2) ◽  
pp. 234 ◽  
Author(s):  
A. C. S. Castilho ◽  
C. A. Price ◽  
F. Dalanezi ◽  
R. L. Ereno ◽  
M. F. Machado ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Granulosa Cells ◽  
Mrna Levels ◽  
Fibroblast Growth ◽  
Dominant Follicle ◽  
Significant Difference ◽  
The Future ◽  
Follicle Selection

There is evidence that regulation of follicle selection in cattle involves locally produced growth factors. In the present study, we investigated the expression of members of the fibroblast growth factor (FGF) 7 family during follicle deviation. The largest and second largest follicles were recovered during the second day of a synchronised follicle wave and the future dominant and future subordinate follicles were identified based on diameter and cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1) mRNA levels in granulosa cells. Theca cells of the future dominant follicle contained less mRNA encoding FGF7 and FGF10 compared with those from the future subordinate follicle 2.5 days after ovulation, before a significant difference between the diameters of the future dominant and future subordinate follicles could be observed, but FGF22 mRNA levels did not change. Levels of mRNA encoding FGF receptors FGFR1B and FGFR2B in theca and granulosa cells, respectively, were lower in the future dominant follicle compared with the future subordinate follicle. Addition of FGF10 to granulosa cells in vitro significantly decreased oestradiol secretion, as well as CYP19A1, FSH receptor (FSHR) and insulin-like growth factor 1 receptor (IGF1R) mRNA abundance, whereas FGF22 had no effect. We conclude that FGF10 and FGFR2B expression is increased in the future subordinate follicle before morphological deviation, which may contribute to follicle selection.

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