285 THE EFFICIENCY OF INTRACYTOPLASMIC SPERM INJECTION v. LASER-ASSISTED IN VITRO FERTILIZATION WITH FROZEN SPERM FOR RECOVERY TRANSGENIC C57BL/6 MOUSE STRAINS

2011 ◽  
Vol 23 (1) ◽  
pp. 240
Author(s):  
A. C. Carstea ◽  
Z. Polgar ◽  
L. Kovacs ◽  
A. Dinnyes
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Skim Milk ◽  
Mouse Strains ◽  
Chi Square ◽  
Cell Stage ◽  
Important Indicator ◽  
Vitro Fertilization ◽  
Cryopreserved Sperm

The progress of molecular genetics generated thousands of new transgenic strains of mice which also requires their economic and safe maintenance in the form of genetic banks. Sperm freezing would be one of the easiest options; however, cryosensitivity of sperm in mice strains is prone to variation. In this study, we examined the efficiency of laser-assisted zona drilling in vitro fertilization (ZD-IVF) v. intracytoplasmic sperm injection (ICSI) for attempting to recover two transgenic (UBI-GFP/BL6 and B6;129P2- Hvcn1) and one mutant (C57BL/6J-Tyrc-2J) lines. The sperm was frozen with 18% raffinose and 3% skim milk (Nakagata 2000 Mamm Genome 11, 572–576). Data of the replicates was analysed by chi-square method. The motility rates after thawing of cryopreserved sperm were 10% for UBI-GFP/BL6, 30% C57BL/6J-Tyrc-2J and 50% for B6;129P2-Hvcn1 strains. Regular IVF attempts in the UBI-GFP/BL6 and the mutant strain resulted in very few embryos and no pups (data not shown). Following ZD-IVF, the 2-cell stage rates were 7/60 (12%) for UBI-GFP/BL6, 18/60 (30%) for C57BL/6J-Tyrc-2J, and 34/60 (56%) for B6;129P2- Hvcn1. After ICSI, 66–74% of the oocytes survived the procedure and their development to 2-cells stage were 12/20 (60%) (UBI-GFP/BL6), 25/39 (64%) (C57BL/6J-Tyrc-2J) and 26/37 (70%) (B6;129P2-Hvcn1). The number of 2-cell stage embryos produced by ICSI was significantly (P < 0.05) increased compared with those produced following ZD-IVF in case of UBI-GFP/BL6 and C57BL/6J-Tyrc-2J strains. The 2-cell stage embryos were transferred into recipients and the newborn rates from ZD-IVF v. ICSI embryos were 0% v. 17% (UBI-GFP/BL6), 28% v. 36% (C57BL/6J-Tyrc-2J) and 12% v. 12% (B6;129P2- Hvcn1), respectively; none of them were significantly different. In conclusion, when using cryopreserved sperm, the post-thaw motility is an important indicator for the selection of the rederivation method of cryopreserved transgenic mouse strains; while ZD-IVF, an easier method to perform, is suitable for the higher motility samples, ICSI could be strongly recommended for those showing low motility. This work was financed by EU FP6: CLONET (MRTN-CT-2006-035468), TEAMOHOLIC (MEXT-CT-2003-509582); EU FP7: RESOLVE (FP7-HEALTH-F4-2008-202047), RabPStem (PERG07-GA-2010-268422), and NKFP_07_1-ES2HEART-HU (OM-00202-2007).

scholarly journals Hubungan Anti-Mullerian Hormone (AMH) pada Long dan Short Protocol Terhadap Fertilization Rate Pasien In Vitro Fertilization (IVF)

2021 ◽  
Vol 4 (2) ◽  
pp. 151-161
Author(s):  
Rizki Amalia Wahid ◽  
◽  
Edwin Armawan ◽  
ono Djuwantono
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
Fertilization Rate ◽  
Chi Square ◽  
Vitro Fertilization ◽  
Fertility Clinic ◽  
Long Protocol

Abstrak Tujuan: Untuk mengevaluasi pengaruh kadar anti-mullerian hormone (AMH) dengan fertilization rate (FR) dan menilai perbedaan pengaruh jenis protokol (long protocol (LP) dan short protocol (SP)) pada tiap tingkat cadangan ovarium terhadap FR pada pasien in vitro fertilization (IVF) dengan Intracytoplasmic Sperm Injection. Metode: Data sekunder dari rekam medis pasien yang menjalani IVF di Aster Fertility Clinic Rumah Sakit Umum Pendidikan dr. Hasan Sadikin pada tahun 2016-2020 dan Bandung Fertility Centre Rumah Sakit Ibu Anak Limijati pada tahun 2018-2019. Penelitian ini analitik observational dengan metode Cohort retrospektif. Hubungan antara dua data kategorik diuji dengan uji chi-square dan uji Kruskal-Wallis digunakan pada data numerik dengan distribusi yang tidak rata pada lebih dari 2 kelompok, Hasil: Hasil data diperoleh nilai rerata kadar AMH secara keseluruhan adalah 3.30 ng/ml dengan rerata capaian FR sebesar 71.97%. Berdasarkan metode IVF yang dipilih, mayoritas pasien menjalani pengobatan SP 54.4% (rerata FR 72.80%) dibandingkan dengan LP 45.6% (rerata FR 70.97%). Tidak ditemukan hubungan yang bermakna antara kadar AMH dengan FR, dinyatakan dengan nilai p=0.977. Kadar AMH terhadap FR bila dipisahkan menurut protokol terapi yang diberikan tidak menunjukkan perbedaan yang bermakna pada masing-masing protokol (LP p=0,763; SP p=0,843). Mengenai hubungan antara protokol IVF dengan FR juga tidak diperoleh perbedaan yang signifikan secara statistik dengan nilai p=0,27 (RR 1.17 (0.62-2.15); CI 95%). Penggobatan menggunakan LP (p=0,770) maupun SP (p=0.845) tidak memberikan pengaruh yang bermakna terhadap FR pada setiap kategori AMH. Kesimpulan: Tidak ada pengaruh kadar AMH dan protokol terapi terhadap FR. Kata kunci : In Vitro Fertilization, Fertilization Rate, Anti-Mullerian Hormone, Protokol Stimulasi Ovarium

Sign in / Sign up

Export Citation Format

Share Document