56 EFFECTS OF PHYLOGENIC GENERA OF RECIPIENT CYTOPLASTS ON DEVELOPMENT AND VIABILITY OF CANADA LYNX (LYNX CANADENSIS) CLONED EMBRYOS

2010 ◽  
Vol 22 (1) ◽  
pp. 186 ◽  
Author(s):  
M. C. Gómez ◽  
J. I. Lyons ◽  
C. E. Pope ◽  
M. Biancardi ◽  
C. Dumas ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Donor Cell ◽  
Domestic Cat ◽  
Developmental Competence ◽  
Lynx Lynx ◽  
Phylogenetic Distance ◽  
Lynx Canadensis ◽  
Gestational Length ◽  
Canada Lynx ◽  
Oocyte Donor

Canada lynx (Lynx canadensis; CL) once occupied 16 states in the Unites States of America, but small populations remain in only 3 states. Interspecies-somatic cell nuclear transfer (Is-SCNT) offers the possibility of preventing their extinction; however, developmental constraints on Is-SCNT embryos are proportional to the phylogenetic distance between the donor cell and the recipient oocyte. Mitochondrial DNA (mtDNA) heteroplasmy may be involved in nuclear-cytoplasmic incompatibilities, thus inhibiting development of cloned embryos at the time of genomic activation. Minimizing the phylogenetic distance between the donor cell and recipient oocyte may enhance development of clone embryos. Caracal (Caracal caracal) may be suitable as an oocyte donor for SCNT and a recipient of CL cloned embryos because caracals hybridize with other felid species and share physical characteristics with the lynx family, marked by being previously classified in the lynx genera and having similar gestational length. To ensure compatibilities between the donor nuclei of the CL and the mitochondria of recipient oocytes, we (1) compared in vitro development of CL cloned embryos reconstructed with domestic cat (Felis catus; DSH) or caracal cytoplasts, (2) examined the mtDNA genotypes in CL cloned embryos, and (3) evaluated in vivo developmental competence of CL cloned embryos after transfer into caracal recipients. A total of 160 and 217 preovulatory oocytes were collected by laparoscopy from gonadotropin-treated caracals (n = 8) and DSH (n = 10) and used as recipient cytoplasts for reconstructing CL embryos. Results indicated that the phylogenetic genera of recipient cytoplasts did not affect embryo cleavage at Day 2 (caracal 50/55, 91% v. DSH 63/65, 97%), but development of CL cloned embryos to the blastocyst stage was higher when caracal oocytes were used as recipient cytoplasts (15/50; 30%) than with DSH cytoplasts (9/63, 14%; P < 0.05). The extent of mtDNA homoplasmy or heteroplasmy in CL cloned embryos was calculated by the number of single nucleotide polymorphisms (SNP) derived from the DSH or caracal oocyte donors and from the somatic cell donor CL. DNA was isolated from 25 and 35 CL cloned embryos reconstructed with caracal or DSH cytoplasts, respectively. All amplified products after PCR were sequenced and SNP analyzed. All CL embryos reconstructed with DSH cytoplasts were homoplasmic, carrying mtDNA only from the DSH oocyte donor (n = 35; SNP DSH = 2-6). Embryos reconstructed with caracal cytoplasts were homoplasmic for CL mtDNA (n = 9; SNPCL = 10-12) or heteroplasmic (caracal × CL, n = 17; SNPCL = 7-9; SNP caracal = 2-3). A total of 69 (mean = 34.5 ± 4.9 per caracal) and 70 (mean = 35.0 ± 9.8 per caracal) CL cloned embryos reconstructed with caracal and DSH cytoplasts, respectively, were transferred into 4 caracal recipients; however, no pregnancies were established. In summary, Is-SCNT between 2 phylogenetically closer species favors retention of the donor’s mitochondria, which might lead to a better nucleo-cytoplasmic interaction for reprogramming of donor nucleus.

scholarly journals Spatio-temporal responses of Canada lynx (Lynx canadensis) to silvicultural treatments in the Northern Rockies, U.S.

2018 ◽  
Vol 422 ◽  
pp. 114-124 ◽  
Author(s):  
Joseph D. Holbrook ◽  
John R. Squires ◽  
Barry Bollenbacher ◽  
Russ Graham ◽  
Lucretia E. Olson ◽  
...  
Keyword(s):  
Lynx Lynx ◽  
Lynx Canadensis ◽  
Canada Lynx ◽  
Silvicultural Treatments ◽  
Northern Rockies ◽  
Spatio Temporal

49 INTRA-GENUS CLONED EMBRYOS PRODUCTION AND PREGNANCIES DERIVED FROM LEOPARD CAT NUCLEI AND DOMESTIC CAT ENUCLEATED EGGS

2006 ◽  
Vol 18 (2) ◽  
pp. 133 ◽  
Author(s):  
I. K. Kong ◽  
H. S. Lee ◽  
N. H. Kim ◽  
L. H. Kim ◽  
H. D. Shin ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Nuclear Genome ◽  
Fibroblast Cell ◽  
Domestic Cat ◽  
Developmental Competence ◽  
Cell Nuclei ◽  
Reconstructed Embryos ◽  
Significant Difference ◽  
Leopard Cat

The leopard cat (Prionailurus bengalensis), a member of the felidae family, is currently listed as threatened by the Ministry of Environment in South Korea. In exotic or endangered species, the lack of oocytes and recipients precludes the use of traditional somatic cell nuclear transfer (NT), and an approach such as intragenus NT may be the only alternative for producing embryos and offspring. In the present study, we used the leopard cat (LC) as a somatic cell donor to evaluate the in vivo developmental competence, after transfer into domestic cat recipients, of cloned embryos produced by the fusion of LC fibroblast cell nuclei with domestic cat cytoplasts. A total of 412 enucleated domestic cat oocytes were reconstructed with either male (Treatment A) or female (Treatment B) adult LC fibroblasts. There was no significant difference in fusion rate (60.4 vs. 56.9%) between Treatment A and B. Of the fused couplets, the cleavage and blastocyst developmental rate in Treatment A were greater than those in Treatment B (69.5 vs. 60.9%; 8.3 vs. 7.8%; P < 0.05). In treatment A, in vivo developmental studies at 30-45 days postimplantation demonstrated 4.8% (21/435) of reconstructed embryos (n = 435) had entered into the uterine lining of recipients, but only 1.4% (6/435) formed fetuses. However, all of the reconstructed embryos failed to develop to term (65 days). Microsatellite analyses confirmed that the nuclear genome of the cloned fetuses were LC in origin.

17 Effect of the zona-free aggregation on the developmental competence of kodkod (Leopardus guigna) embryos generated by interspecies somatic cell nuclear transfer

2019 ◽  
Vol 31 (1) ◽  
pp. 134
Author(s):  
D. Veraguas ◽  
C. Aguilera ◽  
D. Echeverry ◽  
D. Saez-Ruiz ◽  
F. O. Castro ◽  
...  
Keyword(s):  
Gene Expression ◽  
Somatic Cell ◽  
Blastocyst Stage ◽  
Domestic Cat ◽  
Developmental Competence ◽  
Relative Expression ◽  
Standard Curve ◽  
Morula Stage ◽  
Leopardus Guigna

The kodkod is considered a vulnerable species by the International Union for Conservation of Nature. Phylogenetically, the kodkod is classified in the Leopardus genus, which has only 36 chromosome pairs compared with the domestic cat, which has 38. The proposed hypothesis was that domestic cat oocytes are capable of reprogramming somatic cells from kodkod after interspecies somatic cell NT (SCNT), allowing the in vitro embryo development up to blastocyst stage. Five experimental groups were made based on the technology and culture system: (1) cat embryos generated by IVF (IVF), (2) cat embryos generated by SCNT (Ca1x), (3) aggregated cat embryos generated by SCNT (Ca2x), (4) kodkod embryos generated by interspecies SCNT (K1x), and (5) aggregated kodkod embryos generated by interspecies SCNT (K2x). Interspecies SCNT was performed using a zona-free method. Reconstructed embryos were activated by 2 electrical pulses of 140 kV cm−1 for 40 µs and then incubated for 5h in 10μg mL−1 of cycloheximide and 5μg mL−1 of cytochalasin B. Embryos were cultured in SOF media using the well of the well system in a 5% O2, 5% CO2, and 90% N2 atmosphere at 38.5°C for 8 days. The morulae and blastocysts rates were estimated, and diameter of cloned blastocysts was measured. The relative expression of OCT4, SOX2, and NANOG was evaluated in blastocysts by RT-qPCR using the standard curve method; SDHA was used for normalization. The Kruskal-Wallis test was used to evaluate the developmental parameters and gene expression. The t-test was used to evaluate blastocyst diameter. Statistical differences were considered at P&lt;0.05. The number of replicates was IVF=10, Ca1x=8, Ca2x=6, K1x=3, and K2x=8. The morulae rate was lower when clone embryos were cultured individually (IVF=97/153, 63.4%; Ca2x=28/51, 54.9%; K2x=63/110, 57.3%; Ca1x=48/126, 38.1%; K1x=22/87, 25.3%; P&lt;0.05). In the domestic cat, blastocysts rate was higher in IVF (58/153, 37.9%) and Ca2x (28/51, 29.4%) groups than in the Ca1x group (21/126, 16.7%; P&lt;0.05). No blastocysts were generated in the K1x group (0/87), whereas 5.5% of blastocysts were obtained from the K2x (6/110; 5.5%); this was not statistically different compared with the K1x group (P&gt;0.05). No differences were found in blastocyst diameter between the Ca1x (220.4µm) and Ca2x (251.2µm) groups (P&gt;0.05). However, the diameter of the blastocysts from the K2x group (172.8µm) tended to be lower than that of the blastocysts from the Ca2x group (P=0.05). Regarding gene expression, only 1 of the 6 kodkod blastocysts expressed OCT4, and none expressed SOX2 and NANOG. On the other hand, the relative expression of OCT4 tended to decrease in blastocysts from the Ca1x and Ca2x groups compared with the IVF group (P=0.09), but no differences were found in the expression of SOX2 and NANOG among groups (P&gt;0.05). In conclusion, after interspecies SCNT, domestic cat oocytes support the development of kodkod embryos until the morula stage. However, the embryo aggregation did not significantly improve the blastocyst rate and gene expression.

scholarly journals Evaluating evolutionary history and adaptive differentiation to identify conservation units of Canada lynx (Lynx canadensis)

2019 ◽  
Vol 20 ◽  
pp. e00708
Author(s):  
Melanie B. Prentice ◽  
Jeff Bowman ◽  
Dennis L. Murray ◽  
Cornelya FC. Klütsch ◽  
Kamal Khidas ◽  
...  
Keyword(s):  
Evolutionary History ◽  
Lynx Lynx ◽  
Lynx Canadensis ◽  
Conservation Units ◽  
Canada Lynx ◽  
Adaptive Differentiation

Latitudinal gradient in cortisol concentrations in Canada lynx (Lynx canadensis) is not explained by diet

2019 ◽  
Vol 97 (8) ◽  
pp. 748-753
Author(s):  
C.M. Burstahler ◽  
C.V. Terwissen ◽  
J.D. Roth
Keyword(s):  
Stable Isotope ◽  
Food Limitation ◽  
Isotope Ratios ◽  
Stress Axis ◽  
Stable Isotope Ratios ◽  
Lynx Lynx ◽  
Alternative Prey ◽  
Lynx Canadensis ◽  
Canada Lynx

Food limitation is an important stressor for most wildlife, and many specialist consumers will expand their dietary niche to contend with preferred prey limitation. How these dietary responses feed back into stress-axis regulation, however, is unknown. If alternative prey does not sufficiently fill the energetic requirements normally satisfied by preferred resources, then long-term glucocorticoid concentrations could be elevated in individuals consuming alternative prey. We measured cortisol concentrations and stable isotope ratios (δ13C and δ15N) in hair of Canada lynx (Lynx canadensis Kerr, 1792) across their distribution to determine the influence of diet on glucocorticoids while controlling for harvest location. We calculated the Euclidean distance between lynx and regional snowshoe hare (Lepus americanus Erxleben, 1777) stable isotope ratios as an index of diet specialization. We found no relationship between this index and cortisol, suggesting that prey types are interchangeable for lynx in terms of long-term stress axis activation. However, lynx cortisol increased significantly towards the northwestern region of lynx distribution, contrasting with our prediction, and highlighting important considerations for future research. This combination of glucocorticoid and diet analyses suggests that dietary plasticity does not necessarily alter an individual’s experience of potential stressors, despite important implications to population and community dynamics.

scholarly journals Spatial and environmental influences on selection in a clock gene coding trinucleotide repeat in Canada lynx (Lynx canadensis)

2020 ◽  
Vol 29 (23) ◽  
pp. 4637-4652
Author(s):  
Melanie B. Prentice ◽  
Jeff Bowman ◽  
Dennis L. Murray ◽  
Kamal Khidas ◽  
Paul J. Wilson
Keyword(s):  
Clock Gene ◽  
Trinucleotide Repeat ◽  
Environmental Influences ◽  
Lynx Lynx ◽  
Lynx Canadensis ◽  
Canada Lynx ◽  
Gene Coding

Patterns of ovarian and luteal activity in captive and wild Canada lynx (Lynx canadensis)

2010 ◽  
Vol 169 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Kerry V. Fanson ◽  
Nadja C. Wielebnowski ◽  
Tanya M. Shenk ◽  
Jennifer H. Vashon ◽  
John R. Squires ◽  
...  
Keyword(s):  
Lynx Lynx ◽  
Lynx Canadensis ◽  
Canada Lynx

scholarly journals Serologic Survey for Viral and Bacterial Infections in Western Populations of Canada Lynx (Lynx canadensis)

2002 ◽  
Vol 38 (4) ◽  
pp. 840-845 ◽  
Author(s):  
Roman Biek ◽  
Randall L. Zarnke ◽  
Colin Gillin ◽  
Margaret Wild ◽  
John R. Squires ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Lynx Lynx ◽  
Lynx Canadensis ◽  
Canada Lynx ◽  
Serologic Survey

Wandering Canada lynx (Lynx canadensis) in Kansas

2008 ◽  
Vol 111 (1 &amp; 2) ◽  
pp. 161-162 ◽  
Author(s):  
Curtis J. Schmidt ◽  
Jerry R. Choate ◽  
Kendra L. Phelps
Keyword(s):  
Lynx Lynx ◽  
Lynx Canadensis ◽  
Canada Lynx
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