420 SUPERSTIMULATION OF ANGUS DONORS WITH A SINGLE INTRAMUSCULAR INJECTION OF FOLLTROPIN®-V

2010 ◽  
Vol 22 (1) ◽  
pp. 367 ◽  
Author(s):  
A. Tríbulo ◽  
H. Tríbulo ◽  
R. Tríbulo ◽  
D. Carballo Guerrero ◽  
P. Tríbulo ◽  
...  
Keyword(s):  
Single Injection ◽  
Animal Health ◽  
Treatment Schedule ◽  
Embryo Production ◽  
Release Formulation ◽  
Treatment Protocols ◽  
Single Intramuscular Injection ◽  
Effect Of Treatment ◽  
Estradiol 17Β ◽  
Donor Cows

Two experiments were designed to determine the superovulatory response of Angus donors treated with a single injection of Folltropin®-V diluted in a slow release formulation (SRF; Bioniche Animal Health Inc., Belleville, Ontario, Canada). Experiment 1 was designed to compare superovulatory response in Angus donor cows (n = 29 per group) treated with a single i.m. injection of Folltropin®-V diluted in SRF with cows treated using the traditional twice-daily i.m. injection treatment schedule. On Day 0, all cows received 5 mg of estradiol-17β plus 50 mg of progesterone and a Cue-Mate® (Bioniche Animal Health). On Day 4, cows were superstimulated with 400 mg of NIH-FSH-P1 Folltropin®-V in twice-daily decreasing doses over 4 days or in a single i.m. injection in the neck. The single injection was prepared by diluting the Folltropin®-V lyophilized powder in 1 mL of saline for injection and mixed with 9 mL of the SRF in the syringe immediately before administration. In the am and pm of Day 6, all cows received PGF2 and Cue-Mates® were removed in the pm. In the am of Day 8, cows received 12.5 mg of porcine LH (Lutropin®-V; Bioniche Animal Health) and were inseminated 12 and 24 h later. Ova/embryos were collected nonsurgically on Day 15 and evaluated following IETS recommendations. Means were compared between groups by t-test Mean (±SEM) number of ova/embryos and transferable embryos were 13.7 ± 2.1 and 7.1 ± 1.3 v. 12.3 ± 1.5 and 5.3 ± 0.8 for donors treated with the single v. twice-daily injections, respectively (P > 0.4). Experiment 2 was designed to confirm the results of Experiment 1 and to compare the effect of different dosages of Folltropin®-V on embryo production in Angus cows. Cows (n = 23) were superstimulated by 6 treatment protocols (2 × 3 factorial) in a crossover design (i.e. all cows received the 6 treatments and all treatments were represented on each collection day). Cows received the same treatments as cows in Experiment 1 except that the dosages of Folltropin®-V used were 200, 300, or 400 mg. Statistical analysis (ANOVA) revealed a significant effect of dosage of Folltropin®-V on embryo production, but there was no effect of treatment (i.e. single v. twice-daily injections; P > 0.2), nor was there a treatment by dosage interaction (P > 0.7). Mean (± SEM) number of ova/embryos and transferable embryos were 10.3 ± 0.9 and 5.4 ± 0.6 v. 11.3 ± 0.6 and 5.6 ± 0.5 for donors treated with the single or twice-daily injections, respectively (P > 0.2). Furthermore, the mean number of ova/embryos and transferable embryos were higher in cows treated with 400 mg (13.9 ± 1.1 and 6.5 ± 0.7) and 300 mg (12.0 ± 1.0 and 6.1 ± 0.7) of Folltropin®-V than those treated with 200 mg (6.6 ± 0.7 and 4.0 ± 0.5). In summary, superstimulation of Angus donor cows with a single i.m. injection of Folltropin®-V diluted in an SRF resulted in comparable embryo production to the traditional twice-daily i.m. administration of Folltropin®-V over 4 days. Although response did not differ between 300 and 400 mg, results suggest that 300 mg of Folltropin®-V given i.m. by a single or twice-daily injections is the most appropriate dose for Angus donor cows.

416 DOSE TITRATION FOR SUPERSTIMULATION OF BRANGUS AND BONSMARA DONORS WITH Folltropin®-V BY A SINGLE INTRAMUSCULAR INJECTION

2010 ◽  
Vol 22 (1) ◽  
pp. 365 ◽  
Author(s):  
D. Rogan ◽  
A. Tríbulo ◽  
H. Tríbulo ◽  
R. Tríbulo ◽  
D. Carballo Guerrero ◽  
...  
Keyword(s):  
Animal Health ◽  
Crossover Design ◽  
Dose Titration ◽  
Embryo Production ◽  
Release Formulation ◽  
Single Intramuscular Injection ◽  
Effect Of Treatment ◽  
The Mean ◽  
Estradiol 17Β ◽  
Donor Cows

Two experiments were designed to evaluate the superovulatory response of Brangus and Bonsmara donor cows to different dosages of Folltropin®-V (Bioniche Animal Health Inc., Belleville, Ontario, Canada) given by a single i.m. injection or twice-daily i.m. injections. In Experiment 1, Brangus cows (n = 12) were superstimulated by 6 treatments (2 × 3 factorial) in a crossover design (i.e. all cows received the 6 treatments and all treatments were represented on each day). On Day 0, cows received 5 mg of estradiol-17β plus 50 mg of progesterone and a Cue-Mate® (Bioniche Animal Health Inc.). On Day 4, cows were superstimulated with 300, 260, or 200 mg of NIH-FSH-P1 Folltropin®-V (Bioniche Animal Health Inc.) in twice-daily decreasing doses over 4 days or diluted in a slow release formulation (SRF; Bioniche Animal Health) and given in a single i.m. injection. The single injection was prepared by diluting the Folltropin®-V lyophilized powder in 1 mL of saline followed by mixing with 9 mL of the SRF in the syringe immediately before administration. In the am and pm of Day 6, all cows received PGF2, and Cue-Mates® were removed in the pm. Cows received 12.5 mg of porcine LH (Lutropin®-V; Bioniche Animal Health Inc.) in the am of Day 8 and were inseminated 12 and 24 h later. Ova/embryos were collected on Day 15 and data were analyzed by ANOVA. There was no effect of treatment (i.e. single v. twice-daily injections; P > 0.2) nor a treatment by dosage interaction (P < 0.6) on the mean (± SEM) number of total ova/embryos or transferable embryos (13.1 ± 1.9 and 7.5 ± 1.2 v. 15.5 ± 1.7 and 7.6 ± 1.0 for single v. twice-daily injections, respectively). The total number of ova/embryos did not differ among Folltropin®-V dosages (15.0 ± 2.3, 15.7 ± 2.0, and 12.1 ± 2.5 for 300, 260, and 200 mg, respectively; P > 0.4). However, the number of transferable embryos tended (P < 0.09) to be higher in donors receiving 260 mg (9.5 ± 1.6) than 200 mg (5.2 ± 0.8), with 300 mg (7.9 ± 1.5) intermediate. In Experiment 2, Bonsmara cows (n = 16) were superstimulated by 4 treatments (2 × 2 factorial) in a crossover design similarly to Experiment 1, except that 2 dosages of Folltropin®-V (200 and 300 mg) were evaluated. There were no significant effects of dosage of Folltropin®-V (P > 0.9), treatment (P > 0.3), or interaction (P < 0.4) on embryo production. The total number of ova/embryos and transferable embryos were 11.9 ± 2.0 and 7.2 ± 1.1 v. 11.1 ± 1.1 and 7.6 ± 0.7 for single and twice-daily injections, respectively, and 11.9 ± 1.9 and 7.6 ± 1.0 v. 11.1 ± 1.3 and 7.2 ± 0.8 for 300 and 200 mg of Folltropin®-V, respectively. Superstimulation of Brangus and Bonsmara cows with a single i.m. injection of Folltropin®-V diluted in a SRF resulted in comparable embryo production to twice-daily administration of Folltropin®-V over 4 days. While 260 mg seems to be the most appropriate dosage for Brangus donors, 200 mg seems to be adequate for Bonsmara donors.

New approaches to superovulation in the cow

2010 ◽  
Vol 22 (1) ◽  
pp. 106 ◽  
Author(s):  
Gabriel A. Bó ◽  
Daniel Carballo Guerrero ◽  
Andrés Tríbulo ◽  
Humberto Tríbulo ◽  
Ricardo Tríbulo ◽  
...  
Keyword(s):  
Intramuscular Injection ◽  
Animal Health ◽  
Embryo Production ◽  
Release Formulation ◽  
Widespread Application ◽  
Handling Stress ◽  
Follicular Wave ◽  
Mechanical Removal ◽  
Single Intramuscular Injection ◽  
Gonadotrophin Releasing Hormone

There is continuing need to simplify bovine superovulation protocols without compromising embryo production. The control of follicular wave emergence and ovulation has facilitated donor management, but the most commonly used treatment, oestradiol, cannot be used in many parts of the world and mechanical removal of the dominant follicle is difficult to apply in the field. Other alternatives include gonadotrophin-releasing hormone (GnRH) or LH, but efficacy in groups of randomly cycling animals is variable. Another alternative is to increase the response to GnRH by inducing a persistent follicle and initiating FSH treatments following GnRH-induced ovulation. The number of transferable embryos following superovulation during the first follicular wave did not differ from that achieved 4 days after oestradiol benzoate and progesterone. To further simplify superovulation, FSH has been administered as a single intramuscular injection. Superovulation of beef donors with a single intramuscular injection of Folltropin-V (Bioniche Animal Health, Belleville, ON, Canada) diluted in a slow-release formulation resulted in embryo production comparable to that obtained using the traditional twice-daily protocol. The single intramuscular injection has the potential to reduce labour and handling and may be useful when handling stress is an impediment to success. These alternatives provide ways of facilitating widespread application of embryo transfer technologies.

364 DISTRIBUTION OF OVULATION AND SUBSEQUENT EMBRYO PRODUCTION USING LUTROPIN AND ESTRADIOL-17β FOR TIMED AI OF SUPERSTIMULATED BEEF FEMALES

2006 ◽  
Vol 18 (2) ◽  
pp. 289 ◽  
Author(s):  
W. Larkin ◽  
P. Chesta ◽  
C. Looney ◽  
G. Bo ◽  
D. Forrest
Keyword(s):  
Animal Health ◽  
Transrectal Ultrasound ◽  
Beef Cows ◽  
Control Treatment ◽  
Embryo Production ◽  
Ultrasound Evaluation ◽  
Frozen Semen ◽  
Prostaglandin F ◽  
Estradiol 17Β ◽  
Post Onset

This experiment was designed to compare timing of ovulation and embryo production between traditional superstimulatory and AI methods and the use of additional treatments of Estradiol-17β and pLH (Lutropin; Bioniche Animal Health, Belleville, Ontario, Canada) to facilitate timed AI in beef cows. A total of 31 mature cross-bred beef cows were randomly assigned three treatments. On Day 0, experimental cows were selected upon transrectal ultrasound evaluation for corpus leutum (>10 mm) prior to CIDR insertion plus 2.5 mg estradiol-17β and 50 mg Progesterone i.m. Superstimulatory treatments with Folltropin-V (Bioniche Animal Health) began on Day 4 for 3.5 days (236 mg NIH-FSH-P10) in decreasing dosages (7 injections at 0700 and 1900). All cows received prostaglandin F (PGF) treatments of 625 mcg D-cloprostenol i.m. (Estrumate; Schering-Plough, Union, NJ, USA) in both AM and PM on Day 6, and CIDRs were removed in AM of Day 7, followed by the final injection of Folltropin. In addition, Heat Watch patches (Heat Watch; Cow Chips, Denver, CO, USA) were applied to optimize heat detection. Donors selected as control treatment were AI at 12 and 24 h post-onset of estrus (first mount) with frozen semen from the same bull. Donors selected on the Estradiol-17β treatment received 1 mg i.m. 12 h after CIDR removal and timed AI at 24 and 36 h. Donors selected for Lutropin treatment received 12.5 mg (5cc i.m.) 24 h after CIDR removal and were timed AI at 12 and 24 h. All AI procedures used 2 units from the same bull. All cows were examined by ultrasonography every 8 h beginning 24 h post-CIDR removal and ending at 60 h to determine the time and distribution of ovulation. Ovulation was determined by the disappearance of follicles (>12 mm) from the prior examination. All AI and ultrasound procedures were performed by the same technician. There were no differences detected between the three treatments as evaluated by all the variables we studied (Table 1). However, donors treated with Lutropin tended to produced more viable embryos per collection and had a tighter degree of distribution of ovulations. Donors treated with Estradiol-17β tended to have fewer viable embryos. More research is needed to determine if donors can be timed AI without regard to estrus. Table 1. Mean onset of estrus, distribution of ovulation, and embryo production in superstimulated beef donors treated with Estradiol-17β, and Lutropin

126 A Comparison of Two Different Follicular Coasting Periods for In Vitro Embryo Production in Indian Nelore Cattle

2018 ◽  
Vol 30 (1) ◽  
pp. 203
Author(s):  
P. Tiwari ◽  
S. Zawar ◽  
J. H. Pryor ◽  
C. R. Looney ◽  
R. Kaushik ◽  
...  
Keyword(s):  
Animal Health ◽  
Bos Indicus ◽  
Embryo Production ◽  
Treatment Groups ◽  
Lactating Cows ◽  
Oocyte Recovery ◽  
Minimum Interval ◽  
Nelore Cattle ◽  
Donor Cows

Ongole, also known as Nelore (Bos indicus) cattle, are indigenous to the Andhra region in the Prakasam District in the State of Andhra Pradesh in India. A better understanding and utilisation of follicular wave dynamics within this breed would ultimately enhance oocyte and potential embryo production. Therefore, the aim of this study was to evaluate the differences between coasting periods of 24 h (S1) and 36 h (S2) on oocyte recovery, the rate of viable oocytes, cleavage, and Day 7 blastocyst rates of Nelore cattle in India. A total of 58 ovum pick-up (OPU) sessions (29 per treatment) were performed on 32 healthy donor cows that were randomly assigned to 1 of 2 coasting treatments (S1 or S2). Donors were stimulated as follows: 2.5 mL of gonadotropin-releasing hormone (GnRH; Receptal, MSD Animal Health, New Zealand) given IM on Day 1 followed by once-daily descending dose of Folltropin® (FSH, Vetoquinol, Canada) on Days 3 to 5 for a total of 180 mg. Cumulus-oocyte complexes were collected following OPU on Day 6 either at 24 (S1) or 36 h (S2) following the last FSH injection (coasting period). Donors were subject to OPU 1 to 3 times with a minimum interval of 15 days between procedures from March to April 2017. All 32 donor cows were non-lactating at the time of aspiration and divided equally between treatment groups. A total of 1492 follicles produced 850 total oocytes with oocyte recovery numbers for treatments S1 and S2 (785, 707; 441, 409; respectively). All data were analysed by ANOVA (P < 0.05). The mean number of follicles aspirated from S1 (27 ± 20.2) was not significantly different from that of S2 (24.4 ± 14.4). For S1, 393/441 (89%) quality oocytes were utilised for culture compared with 323/409 (78.9%) for S2, with no differences between rates. Additionally, there were no differences between mean number of oocytes, cleaved embryos, and blastocysts for S1 (15.2 ± 12.7; 9.9 ± 9.2; 4.3 ± 5.4) and S2 (14.1 ± 10; 7.4 ± 6.0; 3.6 ± 3.3; respectively). In conclusion, there were no differences found between 24- or 36-h coasting periods of Nelore cattle undergoing OPU for follicle counts, oocyte recovery, viable oocyte rates, cleavage, and blastocyst rates. Further research is needed to determine whether different stimulation protocols, the use of lactating cows, or coasting periods could alter outcomes.

226 INCLUSION OF BOVINE SOMATOROPIN ON MULTIPLE-OVULATION EMBRYO TRANSFER TREATMENTS FOR LOW-RESPONDING BEEF DONOR COWS

2012 ◽  
Vol 24 (1) ◽  
pp. 225
Author(s):  
S. Kmaid ◽  
J. M. Saldaña ◽  
Z. Ramos ◽  
R. Ungerfeld
Keyword(s):  
Animal Health ◽  
Control Treatment ◽  
Embryo Production ◽  
Multiple Ovulation ◽  
Oestradiol Benzoate ◽  
Pfizer Animal Health ◽  
Universal Laboratory ◽  
To Receive ◽  
Test Treatment ◽  
Donor Cows

An experiment was designed to determine if embryo production of Angus donors could be improved by including a single dose of bovine somatotropin (bST) before starting the superovulatory treatment (SPO). Thirty-three multiparous Angus cows (body condition = 4.6 ± 0.6, range = 1–8) were submitted to 2 SPO treatments at random stages of the oestrous cycle in a crossover design (60 days apart). On Day 0, cows were divided at random to receive either 500 mg of bST (n = 18; Lactotropin, Elanco Saude Animal, Brasil) or remain without bST (control, n = 15). At the same time, all cows received 2 mg of oestradiol benzoate, 50 mg of progesterone and a CIDR (Pfizer Animal Health, Uruguay). On Day 2, all cows received 200 IU of eCG (Inducel, Universal Laboratory, Uruguay) and on Day 4 were superstimulated with 425 IU of FSH (Pluset, Calier, Spain) in twice-daily decreasing doses over 4 days. On Day 6, all cows received 2 doses (a.m. and p.m.) of a PGF analogue (800 μg, Delprostenate, Glandinex, Universal Laboratory, Uruguay) and the CIDRs were removed in the morning of Day 7. Cows were injected with 10 μg of GnRH (Receptal, Intervet, the Netherlands) on Day 8 (a.m.) and were inseminated 12, 24 and 36 h later. On Day 15, ova/embryos were collected nonsurgically and evaluated in accordance to IETS guidelines. For statistical analysis, cows were categorized according to the number of transferable embryos obtained in control treatment (without bST) in terciles: low-responding cows (LR, ≤6; n = 11), middle-responding cows (7–9, n = 12), or high-responding cows (HR ≥ 10; n = 10). Data were analysed by a paired t-test. Treatment with bST increased the total ova and embryos and the number of transferable embryos in LR cows (Table 1). However, the number of transferable embryos and the percentage of transferable embryos werereduced in HR cows. The inclusion of a single bST dose 4 days before initiating SPO with FSH treatments may be a useful alternative to improve embryo production in low-responding cows. Table 1.Response of low-responding and high-responding Angus cows to superovulatory treatments with or without bST (mean ± SD)

207 OOCYTE RECOVERY RATES AND IN VITRO BLASTOCYST PRODUCTION IN CATTLE TREATED WITH A SINGLE INJECTION OF FOLLTROPIN-V DILUTED IN A SLOW-RELEASE FORMULATION

2011 ◽  
Vol 23 (1) ◽  
pp. 202 ◽  
Author(s):  
F. L. Ongaratto ◽  
A. Tríbulo ◽  
M. Ramos ◽  
P. Rodriguez ◽  
G. A. Bó
Keyword(s):  
Slow Release ◽  
Single Injection ◽  
Animal Health ◽  
Prostaglandin F2α ◽  
Release Formulation ◽  
Treatment Groups ◽  
Slow Release Formulation ◽  
Group 3 ◽  
The Mean

We have previously shown that superstimulation of beef donors with a single intramuscular (IM) injection of Folltropin-V (Bioniche Animal Health, Belleville, Ontario, Canada) diluted in a proprietary slow-release formulation (SRF; Bioniche Animal Health) results in in vivo embryo production comparable with the traditional twice-daily IM administration over 4 days (Tribulo et al. 2010 Reprod. Fertil. Dev. 22, 367). Two experiments were designed to evaluate oocyte recovery and in vitro blastocyst production of oocyte donors superstimulated with Folltropin-V, administered in twice-daily injections or diluted in SRF and given by a single injection. In Experiment 1, crossbred beef cows (n = 11) and heifers (n = 5) were randomly allocated to 3 treatment groups in a crossover design (i.e. all animals were treated 3 times, and all treatments were represented in the 3 replicates). All donors had all follicles >8 mm in diameter ablated by ultrasound-guided follicle aspiration on Day 0, received 500 μg of cloprostenol (prostaglandin F2α, Ciclase, Syntex, Argentina), and were treated as follows: Group 1 (multiple FSH): 160 mg of Folltropin-V divided into 4 IM injections administered twice daily (i.e. Days 1 and 2); Group 2 (single FSH): 160 mg of Folltropin-V diluted in 4 mL of 25% SRF and given by a single IM injection in the neck on Day 1; Group 3 (control): no FSH treatment. On Day 4, oocytes were aspirated from all follicles >3 mm in diameter by ultrasound-guided follicular aspiration, classified, matured in TCM-199 supplemented with 0.4% BSA, fertilized in Fert medium, and in vitro cultured in SOF supplemented with 0.4% BSA under oil at 37°C, 5% CO2, 5% O2, 90% N2, and saturated humidity for 7 days. Data were analyzed by ANOVA. The mean (±SEM) numbers of follicles aspirated and oocytes recovered were higher (P < 0.05) in Groups 1 (multiple FSH, 13.6 ± 1.9 and 8.5 ± 1.0) and 2 (single FSH, 14.7 ± 1.7 and 9.4 ± 1.3) than in Group 3 (control, 8.7 ± 0.9 and 5.8 ± 1.0). The mean number of blastocysts after 7 days in culture was numerically higher (P = 0.2) in Groups 1 (2.1 ± 0.7) and 2 (2.6 ± 0.6) than in Group 3 (1.1 ± 0.4). In Experiment 2, lactating Holstein cows (n = 10) received 2.5 mg of estradiol benzoate and 50 mg of progesterone IM on Day 0. On Day 4, all cows received prostaglandin F2α and were randomly allocated to 2 treatment groups, to receive 200 mg of Folltropin-V divided into 4 twice-daily IM injections or 200 mg of Folltropin-V diluted in 25% SRF and given as a single IM injection. On Day 7, oocytes were collected and treated like those in Experiment 1. The mean (±SEM) numbers of follicles aspirated (21.2 ± 3.5 and 14.4 ± 2.9), oocytes recovered (10.2 ± 1.2 and 13.8 ± 2.9), and blastocysts produced (4.6 ± 0.7 and 6.0 ± 2.0) did not differ for Groups 1 and 2, respectively (P = 0.3). In conclusion, superstimulatory treatment increased the number of cumulus–oocyte complexes available for IVF compared with no treatment, and ovarian superstimulatory treatment may be simplified by the use of a single IM dose of a sustained-release product, compared with multiple doses of a conventional product, without a reduction in the recovery or quality of cumulus–oocyte complexes.

230 SUPEROVULATORY RESPONSE OF BEEF COWS WITH OR WITHOUT A CORPUS LUTEUM AT THE TIME OF INSERTION OF A PROGESTERONE RELEASING DEVICE

2012 ◽  
Vol 24 (1) ◽  
pp. 227
Author(s):  
A. Tríbulo ◽  
J. Garzón ◽  
H. Tríbulo ◽  
R. Tríbulo ◽  
D. Rogan ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Mixed Models ◽  
Animal Health ◽  
Treatment Protocol ◽  
Random Variable ◽  
Beef Cows ◽  
Embryo Production ◽  
Transfer Programs ◽  
Intravaginal Device ◽  
Donor Cows

Commercial embryo transfer programs require frequent superovulation of embryo donors. Although early reports suggested that donor cows require 60 to 90 days to recover from superovulation, recent information suggests that this interval could be reduced to 25 to 30 days. Because donor cows reovulate at variable intervals after embryo collection, some donors do not have an ultrasonically detectable corpus luteum (CL) when frequent superovulation programs are initiated. A retrospective study was performed to evaluate the influence of the presence of a CL at the time of initiating treatments on superovulatory response of beef donors that had been previously collected once and then were collected every 28 to 35 days 2 or 3 times. The data were from 190 collections performed in 48 Angus, 36 in 10 Brangus and 74 in 20 Bonsmara donors. On Day 0, all cows were ultrasonically examined for the presence of a CL (Falco 100 Vet, 8-MHz transducer, Pie Medical, the Netherlands) and were treated with 5 mg of oestradiol-17β and 50 mg of progesterone IM and a progesterone-releasing intravaginal device (Cue-Mate, Bioniche Animal Health, Belleville, Ontario, Canada). On Day 4, donors were superstimulated with Folltropin-V (Bioniche Animal Health), in twice-daily injections over 4 days or diluted in 20 mg mL–1 hyaluronan and given by a single IM injection. Folltropin-V dosages used were 300 or 400 mg (Angus), 260 or 300 mg (Brangus), or 200 or 300 mg (Bonsmara). On Day 6, all cows received 2 doses of PGF2α 12 h apart and Cue-Mates were removed in the p.m. In the a.m. of Day 8, cows received 12.5 mg pLH (Lutropin-V; Bioniche Animal Health) and were inseminated 12 and 24 h later. Ova/embryos were collected nonsurgically on Day 15 and evaluated following IETS guidelines. For each breed, superovulatory response (i.e. mean number of CL) and embryo data were evaluated by ANOVA for mixed models, using CL, FSH dose and treatment as fixed variables and cow identification as a random variable. Forty-six (24.2%) Angus donors, 4 (11.1%) Brangus donors and 5 (6.8%) Bonsmara donors did not have a CL at the time of initiating FSH treatment. There was no significant effect of FSH dose or treatment protocol on superovulatory response and the presence or absence of a CL did not influenced embryo production (P > 0.31). Mean (± SEM) number of CL, ova/embryos and transferable embryos were 13.2 ± 0.5, 12.4 ± 0.7 and 6.1 ± 0.4 in Angus donors with a CL vs 13.0 ± 0.7, 11.5 ± 0.9 and 5.6 ± 0.7 for those without a CL (P > 0.37); 14.8 ± 1.2, 14.2 ± 1.6 and 8.4 ± 1.1 for Brangus donors with a CL vs 15.2 ± 3.1; 11.2 ± 3.3 and 5.7 ± 2.8 for those without a CL (P > 0.34); and 13.2 ± 0.8, 11.2 ± 1.0 and 7.4 ± 0.5 for Bonsmara donors with a CL vs 17.4 ± 3.7, 13.6 ± 6.5 and 9.2 ± 4.3 for those without a CL (P > 0.16). In summary, the presence or absence of a CL did not affect embryo production in donor cows superstimulated every 28 to 35 days using a progesterone-releasing device and FSH. Study was supported by Bioniche Animal Health, Belleville, Ontario, Canada.

291 SUPEROVULATORY RESPONSE IN BEEF CATTLE TREATED DURING THE FIRST FOLLICULAR WAVE FOLLOWING SYNCHRONIZATION OF OVULATION WITH A PROGESTIN DEVICE AND GnRH

2009 ◽  
Vol 21 (1) ◽  
pp. 242 ◽  
Author(s):  
D. Carballo Guerrero ◽  
A. Tríbulo ◽  
R. Tríbulo ◽  
H. Tríbulo ◽  
G. A. Bó
Keyword(s):  
Animal Health ◽  
Treatment Protocol ◽  
Crossover Design ◽  
Control Group ◽  
Embryo Production ◽  
Treatment Protocols ◽  
Follicular Wave ◽  
Group 2 ◽  
Student’S T ◽  
Group 1

Although we have previously shown that superstimulation during the first follicular wave resulted in a successful response (Carballo Guerrero D et al. 2007 Reprod. Fertil. Dev. 20, 226), the protocol required many interventions that could influence its application in the field. Therefore, two studies were designed to simplify the superstimulation treatment protocol. Experiment 1 was designed to determine whether it was necessary to remove the progesterone releasing device during the superstimulation protocol. Angus cows (n = 37) were superstimulated by two treatments in a crossover design. Cows in Group 1 (control) received a progesterone releasing device (Cue-Mate, Bioniche Animal Health, Belleville, ON, Canada) along with 0.150 mg D cloprostenol (PGF, Bioprost-D, Biotay, Argentina) IM, at random stages of the estrous cycle. Five days later, a second PGF was injected and Cue-Mates were removed, followed by GnRH (0.050 mg Lecirelina, Biosin-OV, Biotay) 36 h later; ovulation was expected to occur 30 to 36 h later. On Day 0 (36 h after GnRH) donors received a new Cue-Mate and superstimulation treatment was initiated with a total dose of 400 mg NIH-FSH-P1 of Folltropin-V (FSH, Bioniche Animal Health) in twice daily decreasing doses over 5 days. PGF was injected with the last two FSH injections and Cue-Mates were removed with the last FSH injection. Cows in Group 2 were treated similarly to those in the control group, except that Cue-mate devices were not replaced and remained in place for 13 days (i.e. Cue-mates were removed with the last FSH and PGF injection). All donors received 12.5 mg pLH (Lutropin-V, Bioniche Animal Health) 24 h after Cue-Mate removal and were AI 12 and 24 h later. Embryos were collected 7 days after pLH. Means were compared between groups by Student’s t-test. Superovulatory response and embryo production did not differ between groups. Mean (± SEM) number of ova/embryos collected and transferable embryos were 8.2 ± 1.0 and 4.1 ± 0.6 v. 9.8 ± 0.9 and 5.7 ± 0.7 for Groups 1 and 2, respectively (P > 0.2). Experiment 2 was designed to evaluate the effect of giving FSH for 4 v. 5 days. Simmental (n = 18) and Angus (n = 6) cows were superstimulated by the two treatment protocols in a crossover design. Cows in both groups were treated similarly to those in Group 2 in Experiment 1 (i.e. Cue-Mates were not replaced during treatment). Cows in Group 1 (control) received FSH over 5 days (as in Group 2 of Experiment 1); while those in Group 2 received the same dosage of FSH, but given in twice daily decreasing doses over 4 days (Cue-Mates were removed with the last FSH and PGF injections). Superovulatory response and embryo production did not differ among groups. Mean (± SEM) number of ova/embryos collected and transferable embryos were 13.5 ± 2.4 and 6.6 ± 1.1 v. 12.0 ± 1.9 and 5.8 ± 1.0 for Groups 1 and 2, respectively (P > 0.6). In conclusion, superstimulation of cattle at the time of emergence of the first follicular wave after ovulation results in an acceptable superovulatory response and all treatments evaluated were user-friendly and equally efficient.

scholarly journals Effects of coated and noncoated steroidal implants on growth performance, carcass characteristics, and serum estradiol-17β concentrations of finishing Holstein steers

2020 ◽  
Vol 4 (4) ◽  
Author(s):  
Pedro H V Carvalho ◽  
Mariana F Westphalen ◽  
Jonathan A Campbell ◽  
Tara L Felix
Keyword(s):  
Growth Performance ◽  
Animal Health ◽  
Average Daily Gain ◽  
Carcass Characteristics ◽  
Feed Ratio ◽  
Serum Estradiol ◽  
Trenbolone Acetate ◽  
Coated Implant ◽  
Daily Gain ◽  
Estradiol 17Β

Abstract The objectives of the study were to determine the effect of coated or noncoated hormone implants on growth performance, carcass characteristics, and serum estradiol-17β (E2) concentrations of Holstein steers fed a grain-based diet for 112 d. Seventy-nine Holstein steers [average initial body weight (BW) = 452 ± 5.5 kg] were stratified by BW and allotted to one of two treatments: 1) Holstein steers implanted with a coated implant containing 200 mg of trenbolone acetate (TBA) and 40 mg E2 (Revalor-XS (Merck Animal Health; Summit, NJ)] on day 0 (XS) or 2) Holstein steers implanted two times (days 0 and 56) with a noncoated implant containing 80 mg of TBA and 16 mg of E2 [(2IS) Revalor-IS (Merck Animal Health)]. Data were analyzed using the MIXED procedure of SAS (SAS Inst. Inc., Cary, NC). There was no effect (P ≥ 0.71) of implant strategy on initial, middle, and final BW. No effect (P ≥ 0.12) of implant strategy was observed on average daily gain, dry matter intake, or gain-to-feed ratio. There were no effects (P ≥ 0.11) of implant strategy on carcass characteristics. There was an implant × day interaction (P &lt; 0.01) for the circulation of serum E2 concentrations. Serum E2 concentration increased similarly 14 d after Holstein steers were implanted, regardless of implant strategy. At 28 d, after steers were implanted, steers in the XS group had less serum E2 concentration than Holstein steers in the 2IS group. However, at 56 d after the first implantation, both groups, once again, had similar serum E2 concentrations and E2 concentrations were less on day 56 than day 28 for both strategies. Holstein steers implanted with 2IS had greater serum E2 concentration on day 70 and E2 concentrations remained greater than serum E2 of Holstein steers implanted XS for the duration of the trial (day 112). In summary, there was no effect of coated or two doses of noncoated implant on growth performance or carcass characteristics of Holstein steers.

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