29 SHORT-TERM v. LONG-TERM PROGESTERONE PROTOCOL USING CERVICAL OR INTRAUTERINE FIXED-TIME INSEMINATION IN SHEEP

2010 ◽  
Vol 22 (1) ◽  
pp. 172
Author(s):  
A. Menchaca ◽  
M. Vilariño ◽  
E. Rubianes
Keyword(s):  
New York ◽  
Pregnancy Rate ◽  
Body Condition Score ◽  
Skim Milk ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Short Term ◽  
Condition Score ◽  
N 93

The short-term protocol with progesterone, prostaglandin F2α (PGF2α), and eCG is used to control follicular dynamics and luteal activity synchronizing the ovulation for fixed-time AI in sheep. The objective of this experiment was to compare the pregnancy rate obtained with short-term protocol (6 d) and long-term protocol (14 d) using cervical or intrauterine fixed-time AI in sheep. Three hundred fifty-two Merino ewes with a body condition score of 2.9 ± 0.3 (mean ± SD; scale 0 to 5) were used during the breeding season (April, 33S, Uruguay). All the females received a CIDR-G (0.3 g of progesterone, InterAg, Hamilton, New Zealand) for 6 d (short-term protocol; n = 178) or 14 d (long-term protocol, n = 174). One imdose of eCG (300 IU, Novormon, Syntex, BA, Argentina) was given at the moment of device withdrawal for the both protocols, and one imdose of PGF2α (10 mg of dinoprost, Lutalyse, Pfizer, New York, NY, USA) was given at the end of the short-term protocol to ensure luteolysis. Cervical AI (short-term protocol, n = 85; long-term protocol, n = 104) or intrauterine AI (short-term protocol, n = 93; long-term protocol, n = 70) was performed 48 or 54 h after device withdrawal, using 200 × 106 or 100 × 106 spermatozoa per ewe, respectively. Fresh semen was extended in UHT skim milk (1000 × 106 spermatozoa mL-1) and used within 1 h of collection. Estrus was recorded twice a day for 4 days after device withdrawal using vasectomized males. Pregnancy diagnosis was performed by transrectal ultrasonography 40 d after AI (5.0 MHz, Aloka, Tokyo, Japan). Logistic regression was used to analyze the effect of the treatment (P < 0.05), the AI technique (P < 0.05), and their interaction (P = NS). Pregnancy rate was higher for the short-term than for the long-term protocol, and for intrauterine than for cervical AI (Table 1). The highest pregnancy rate was achieved with short-term protocol using intrauterine AI (54.8%, 51/93), and the lowest response was obtained with long-term protocol using cervical AI (33.7%, 35/104; P < 0.05). These data were not different from data of short-term protocol using cervical AI or long-term protocol using intrauterine AI (42.4%, 36/85; and 44.3% 31/70, respectively). Ewes in estrus/treated ewes was not different among short-term and long-term protocols (83.7%, 149/178; and 82.8%, 144/174, respectively; P = NS). In summary, regardless of insemination technique, short-term protocol of 6 d enhances pregnancy rate in fixed-time AI programs in sheep. Table 1.Main effects of short-term (6 d) v. long-term (14 d) protocol using cervical or intrauterine fixed-time AI on pregnancy rate in sheep Financially supported by Pfizer, SP, Brazil.

scholarly journals Effect of an injectable trace mineral at the initiation of a 14 day CIDR protocol on heifer performance and reproduction1

2017 ◽  
Vol 1 (4) ◽  
pp. 458-466 ◽  
Author(s):  
R. S. Stokes ◽  
A. R. Ralph ◽  
A. J. Mickna ◽  
W. P. Chapple ◽  
A. R. Schroeder ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Body Condition Score ◽  
Management Strategies ◽  
Pregnancy Rates ◽  
Variable Response ◽  
Trace Mineral ◽  
Mineral Supplement ◽  
Condition Score ◽  
Calving Season ◽  
N 93

Abstract Three experiments were conducted at separate locations to determine the effects of a trace mineral injection (TMI), Multimin 90, on heifer performance and reproduction. In Exp. 1, [spring-born, Angus, n = 93, body weight (BW) = 428 ± 45.2 kg], Exp. 2 (spring-born, Angus × Simmental, n = 120, BW = 426 ± 54.0 kg), and Exp. 3 (fall-born, commercial Angus, n = 199, BW = 345 ± 39.7 kg) heifers were stratified by BW within experiment and assigned to 1 of 2 treatments: a control, saline injection, or TMI at a dose of 1 mL/68 kg BW. Free choice mineral, containing Cu, Mn, Se, and Zn formulated to meet or exceed NRC recommendations, was supplemented to heifers. Injections were given 33 d prior to breeding at the initiation of a 14-d controlled internal drug release (CIDR)-prostaglandin protocol. There was no difference (P ≥ 0.37) in BW during Exp. 1. Additionally, there was no difference (P ≥ 0.52) in body condition score (BCS) at initiation or at artificial insemination (AI) and final pregnancy confirmation in Exp. 1; however, a greater (P = 0.03) BCS was noted for control heifers at breeding. Pregnancy rates to timed AI and overall pregnancy rates were also similar (P ≥ 0.74) regardless of treatment. During Exp. 2, BCS and BW did not differ (P ≥ 0.44) across treatments. There was a tendency (P = 0.07) for TMI heifers to have an increased AI pregnancy rate (62 vs. 45%) compared with control heifers despite no difference (P = 0.51) in overall pregnancy rate. In Exp. 3, BW was not different (P ≥ 0.39) across all time points. Also, BCS did not differ (P ≥ 0.45) at initiation, AI, or final pregnancy conformation. Interestingly, there was a tendency (P = 0.10) for TMI heifers to have an increased BCS at the time of breeding compared with control heifers. However, there were no differences (P ≥ 0.50) in AI and overall pregnancy rates. In 1 of 3 experiments, an injectable trace mineral administered 33 d prior to the breeding season in conjunction with a 14-d CIDR protocol, tended to increased AI conception rates of heifers even when adequate trace mineral supplement was provided. The variable response observed across experiments may be caused by differences in breed, calving season, mineral sources, and management strategies.

scholarly journals Using data from large scale farming operations to understand the importance of feeding the ewe to improve whole farm performance

2013 ◽  
pp. 97-102 ◽  
Author(s):  
D.R. Stevens ◽  
G. Young
Keyword(s):  
Large Scale ◽  
East Coast ◽  
Body Condition Score ◽  
Short Term ◽  
Management Decisions ◽  
Use Of Data ◽  
Condition Score ◽  
Farm Performance ◽  
Using Data

The collection and use of data from large scale farming operations provided significant insights into drivers of sheep performance. These drivers included minimum two-tooth liveweight at tupping, ewe condition and pasture cover at lambing and the importance of weaning weight on whole farm performance. Using this data to demonstrate the influence of management decisions resulted in an increase in average lamb liveweight gain between birth and weaning of approximately 20 g/day in Landcorp Farming Ltd East Coast flocks over the 4 years of monitoring. Lambing percentage was harder to change, though individual farms increased lambing percentage by up to 35% by concentrating on increasing feed allocation and maintaining ewe body condition score during winter. Low liveweight in some two-tooth ewes was inversely related to the percentage of dries in a flock and prompted more emphasis on growing replacement stock. The programme shifted focus from short-term tactical feeding and management decisions to long-term strategies such as stock and sales policies that placed the breeding flock as the major priority. Keywords: breeding ewes, data, lambing percentage, lambs, liveweight gain, whole flock analysis.

17 PREGNANCY RATE OBTAINED WITH THE REUTILIZATION OF INTRAVAGINAL DEVICE DICO AFTER 6 DAYS OF TREATMENT FOR ESTRUS SYNCHRONIZATION IN SHEEP

2011 ◽  
Vol 23 (1) ◽  
pp. 115
Author(s):  
P. C. dos Santos-Neto ◽  
C. García-Pintos ◽  
A. Menchaca
Keyword(s):  
Pregnancy Rate ◽  
Body Condition Score ◽  
Intrauterine Insemination ◽  
Prostaglandin F2α ◽  
Follicular Development ◽  
Device Removal ◽  
Fertility Rates ◽  
Short Term ◽  
Serum Progesterone ◽  
Intravaginal Device

The new intravaginal device DICO (Dispositivo Intravaginal Caprino Ovino, 0.3 mg progesterone, Syntex, Buenos Aires, Argentina) is effective in controlling serum progesterone concentrations, follicular development and time of ovulation in sheep, even in its second use after 6 days of treatment (Vilariño et al. 2010 Small Rumin. Res. 91, 219–224). The pregnancy rate obtained with DICO in its first use is similar to that obtained with CIDR-G (Pfizer, New York, NY, USA) (abstract of García-Pintos et al. in this issue). However, pregnancy rate with the reutilisation of DICO has not been evaluated yet. The aim of this study was to determine fertility rates in ewes synchronized with a reused DICO in comparison with a reused CIDR-G. The experiment was carried out on 314 Corriedale multiparous ewes during breeding season (March, 33°S, Uruguay), with a body condition score of 3.0 ± 0.1 (scale 0–5). Ewes received a short-term-protocol using DICO (n = 139) or CIDR-G (n = 175) during 6 days, previously used for 6 days. One dose of prostaglandin F2α analogue (125 μg cloprostenol, Ciclase DL, Syntex) and 300 IU eCG (Novormon, Syntex) were given at the time of device removal. Cervical insemination (200 million spermatozoa) or laparoscopic intrauterine insemination (100 million spermatozoa) was performed with fresh semen at fixed-time 48 or 54 h from device removal, respectively. Pregnancy diagnosis was done by transrectal ultrasonography (5 MHz, Well D, Beijing, China) 40 days after insemination. Pregnancy rate was analysed by logistic regression, and number of fetus/pregnant ewes and fetus/inseminated ewes was analysed by Poisson regression. No differences were found between reused DICO and CIDR-G devices (Table 1). Overall, pregnancy rate was affected by the method of insemination (P ≤ 0.05), being lower for cervical (37.0%; 57/154) than intrauterine insemination (55.0%; 88/160). In conclusion, the reutilization of DICO devices in the short-term protocol (6 days of treatment) allows similar fertility rates compared to re-used CIDR-G devices, by both cervical and intrauterine insemination. Table 1.Fertility rate obtained with the reutilisation of DICO and CIDR in short-term protocol (6 days of treatment) in sheep

104 FACTORS AFFECTING PREGNANCY RATES AND EMBRYO/FETAL LOSSES IN RECIPIENTS RECEIVING IN VITRO-PRODUCED EMBRYOS BY FIXED-TIME EMBRYO TRANSFER

2017 ◽  
Vol 29 (1) ◽  
pp. 160
Author(s):  
A. Tribulo ◽  
A. Cedeño ◽  
B. Bernal ◽  
S. Andrada ◽  
J. L. Barajas ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Body Condition Score ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Device Removal ◽  
Releasing Hormone ◽  
Condition Score

A retrospective analysis evaluated pregnancy rates and embryo losses with in vitro-produced embryos in a commercial embryo transfer program on 15 different beef farms. Recipients were beef cows and heifers (n = 1841) that were synchronized with 5 different protocols and transferred at a fixed-time (FTET). Recipients were examined by ultrasonography on Day 0, and those with a corpus luteum (CL) or a follicle ≥8 mm in diameter and with body condition score 2 to 4 (1 to 5 scale) were synchronized. The synchronization treatments were as follows. (T1) Recipients received an intravaginal device with 0.5 g of progesterone plus 2 mg of oestradiol benzoate on Day 0; device removal, plus 500 μg of cloprostenol (prostaglandin F2α), 400 IU of eCG, and 0.5 mg of oestradiol cypionate on Day 8; and FTET on Day 17. (T2) This treatment was similar to T1 but 1 mg of oestradiol cypionate was injected at device removal instead of 0.5 mg of oestradiol cypionate. (T3) This treatment was similar to T1 except that animals were tail-painted on Day 8 and observed on Day 10. Those with the tail-paint intact on Day 10 received 100 μg of gonadorelin (gonadotropin-releasing hormone) and all recipients were FTET on Day 17. (T4) Recipients received a progesterone device on Day 0; device removal, prostaglandin F2α, and eCG on Day 5; gonadotropin-releasing hormone on Day 8; and FTET on Day 15. (T5) Recipients received a progesterone device and 2 mg of oestradiol benzoate on Day 0; device removal, prostaglandin F2α, and eCG on Day 6; gonadotropin-releasing hormone on Day 9; and FTET on Day 16. On the day of FTET all recipients with CL ≥18 mm in diameter (G1), ≥16 and <18 mm in diameter (G2), and ≥14 mm and <16 mm in diameter (G3) received in vitro-produced fresh embryos. Pregnancy was diagnosed by ultrasonography at 30 and 60 days of gestation, and data were analysed by logistic regression. The overall proportion of recipients synchronized that were FTET was 80.8% (1487/1841), with a 30-day pregnancy rate to FTET (P/FTET) of 45.6% (678/1487) and the rate of 30- to 60-day embryo/fetal loses on the 528 recipients that were re-checked at 60 days was 12.8% (68/528). There were no significant differences in P/FTET among operators, animal category, time of the year, embryo stage, or body condition score; however, there was a significant effect of farm (P < 0.001) and CL diameter (P < 0.05), but no interaction between CL diameter and farm or treatment (P > 0.1). Recipients with G1 (443/953, 46%) and G2 (221/462, 47%) CL had higher pregnancy rates than those with G3 CL (23/71, 32%). There was a significant effect of synchronization treatment on the proportion of recipients transferred and on P/FTET (P < 0.01) that was highly influenced by farm (farm by treatment interaction P < 0.01). The proportions of recipients selected for embryo transfer were as follows: T1: 386/486, 79.4%; T2: 233/331, 70.3%; T3: 342/377, 90.7%; T4: 126/160, 78.7%; and T5: 400/487, 82.1%. The P/FTET were as follows: T1: 190/386, 49.2%; T2: 96/233, 41.2%; T3: 175/342, 51.1%; T4: 49/126, 38.8%; and T5: 168/400, 42.0%. Although 30- to 60-day embryo/fetal losses were not influenced by synchronization treatments, they were highly influenced by farm (P < 0.001) and ranged from 0 to 34.5%. In conclusion, P/FTET in a commercial program with beef in vitro-produced embryos was influenced by factors related to the recipient (CL diameter) and the environment (farm), whereas embryo/fetal losses were influenced by farm but not treatment or recipient factors.

scholarly journals Evaluation of Ovsynch Protocol on Reproductive Performance of Anestrous Buffaloes during Breeding Season in Chitwan, Nepal

2017 ◽  
Vol 34 ◽  
pp. 41-50
Author(s):  
C.N. Kharel ◽  
B. Devkota ◽  
S.K. Sah ◽  
R.K. Karki
Keyword(s):  
Pregnancy Rate ◽  
Reproductive Performance ◽  
Body Condition Score ◽  
Fixed Time ◽  
Breeding Technique ◽  
Management Condition ◽  
Internal Genitalia ◽  
Condition Score ◽  
Deposition Intensity ◽  
Ovarian Cyclicity

This study evaluated the effectiveness of Ovsynch/FTAI protocol (day 0 fertirelin inj. 100μg I/M, day 7 cloprostenol inj. 500μg I/M, day 9 fertirelin inj. 100μg I/M, and day 10 fixed time artificial insemination 18 hours after second injection of fertirelin) in anestrous buffaloes of Chitwan, Nepal (n=14). Protocol was evaluated in terms of estrus characteristics, ovulation and pregnancy rate. Responses were analyzed by grouping the buffaloes based on type of anestrus, length of anestrus, body condition score (BCS), milking status, parity, age, management condition, follicle count on day of second GnRH treatment and site of semen deposition. Intensity of estrus characteristics on day of AI was mild. Major estrus signs observed were uterine tonicity, alertness, mucus discharge on rectal manipulation of internal genitalia, swelling of vulva and teat engorgement. Mucus discharge on rectal manipulation of internal genitalia and teat engorgement were clearly noticed in 50 % buffaloes. There was higher tendency (P=0.08) of estrus expression by buffalos with anestrous period of 10 months or lower compared to those with anestrous period longer than 10 months. Overall ovulation rate was 85.7%. Pregnancy rate to FTAI was 28.6% (22.2% and 40% in true anestrous and silent estrus buffaloes). 64.3% (66.7% and 60% of true anestrous and silent estrus) buffaloes were pregnant when checked at 102 days of FTAI including subsequent breeding. Although Ovsynch/FTAI seems to be alternative breeding technique in silent estrus buffaloes, further study with increased sample size is recommended. This protocol can potentially be used for resumption of ovarian cyclicity in true anestrus buffaloes.

scholarly journals The use of insulin to improve fertility of timed-inseminated postpartum suckled beef cows

2010 ◽  
Vol 45 (10) ◽  
pp. 1219-1221 ◽  
Author(s):  
Augusto Schneider ◽  
Luiz Francisco Machado Pfeifer ◽  
Eduardo Schmitt ◽  
Ivan Bianchi ◽  
Marcelo Brandi Vieira ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Body Condition ◽  
Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Body Condition Score ◽  
Insulin Injection ◽  
Fixed Time ◽  
Beef Cows ◽  
Condition Score ◽  
Equine Chorionic Gonadotropin

The objective of this work was to evaluate the effect of insulin alone or in association with equine chorionic gonadotropin (eCG) on the fertility of postpartum beef cows subjected to synchronization. A total of 340 cows was subjected to fixed time artificial insemination. In the trial 1, the cows were subjected to temporary weaning (TW), while in the trial 2 the same protocol was tested without TW. The addition of an insulin injection to a progesterone/eCG-based protocol without TW increased the pregnancy rate of beef cows with body condition score (BCS) equal to or lower than 2.5. Insulin had no effect on cows submitted to TW or with BCS equal to or higher than 3.0.

scholarly journals 9THE USE OF ECG TO INCREASE PREGNANCY RATES IN POSTPARTUM BEEF COWS FOLLOWING TREATMENT WITH PROGESTERONE VAGINAL DEVICES AND ESTRADIOL BENZOATE AND FIXED-TIME AI

2004 ◽  
Vol 16 (2) ◽  
pp. 127 ◽  
Author(s):  
G.A Bo ◽  
L. Cutaia ◽  
P. Chesta ◽  
D. Moreno
Keyword(s):  
Body Condition Score ◽  
Fixed Time ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Control Group ◽  
Treatment Groups ◽  
Condition Score ◽  
N 93 ◽  
Rectal Palpation

Treatments with progesterone (P4) releasing devices and estradiol benzoate (EB) have been extensively used in fixed-time AI (FTAI) programs in beef cattle. However, pregnancy rates in postpartum cows kept on pasture often have been lower than expected because of poor body condition score (BCS) and a high incidence of anestrous. A recent study (Cutaia L et al., 2003 Theriogenology 59, 216) suggested that the addition of eCG to a P4/EB FTAI program may improve pregnancy rates in cows with fair to poor BCS, but results were not conclusive. Therefore, this experiment was designed to further investigate the effect of eCG treatment on pregnancy rates in postpartum beef cows in those conditions. The experiment was performed at 2 locations with lactating Angus cows (n=93), 60 to 90d postpartum with a BCS 1.9 (1 to 5 scale; Location 1), and crossbred Zebu cows (n=290), 60 to 90d postpartum with a BCS 2.0 (Location 2). In each Location, cows were randomly allocated to 1 of the following treatment groups: Control, eCG Day6, or eCG Day8. All cows received a P4 intravaginal device (DIB, Syntex, Argentina) and 2mg EB i.m. (Syntex) on Day0, 500mg cloprostenol (Estroplan, Syntex) at the time of DIB removal (Day8), 1mg EB i.m. on Day9 and FTAI 52 to 56h after DIB removal. Cows in the Control group received no further treatment, whereas cows in the eCG-treated groups received 400 IU eCG (Novormon 5000, Syntex) on Day6 or Day8. Cows were examined on Day0 by rectal palpation (Location 1) or by ultrasonography (US; Location 2) and were classified as those with a CL or without a CL, with either large (&gt;8mm) or small (&lt;8mm)small follicles. Pregnancy was determined by US 45d after FTAI. Data were analyzed by logistic regression and the effects of location, treatment, ovarian status, AI technician and semen were considered in the model. There was no effect of location (P=0.3), AI technician (P=0.2) or semen (P=0.8) on pregnancy rates. However, there was an effect of treatment (P=0.02), attributed to higher pregnancy rates in the eCG Groups than in the Control Group (Table). Furthermore, cows with a CL or without a CL but with large follicles on Day 0 had higher pregnancy rates than those with small follicles (P=0.04). It was concluded that the use of eCG in a P4/EB FTAI program improved pregnancy rates in postpartum beef cows that were in fair topoor BCS.

8 COMBINATION OF OESTRUS DETECTION AND FIXED-TIME ARTIFICIAL INSEMINATION IN BEEF HEIFERS FOLLOWING A SHORTENED OESTRADIOL-BASED PROTOCOL THAT PROVIDES FOR A LENGTHENED PROESTRUS

2015 ◽  
Vol 27 (1) ◽  
pp. 96 ◽  
Author(s):  
J. J. de la Mata ◽  
M. Ré ◽  
G. A. Bó
Keyword(s):  
Pregnancy Rate ◽  
Body Condition Score ◽  
Fixed Time ◽  
Beef Cows ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Group V ◽  
Releasing Hormone ◽  
Treatment Groups ◽  
Condition Score

Studies have shown that gonadotropin-releasing hormone-based protocols that reduce the period of progestin insertion and prolong the period from progestin removal to gonadotropin-releasing hormone and fixed-time AI (FTAI; named 5-day Co-Synch) results in similar or higher pregnancy rates than the conventional 7-day Co-Synch protocol in beef cows and heifers (Bridges et al. 2008 Theriogenology 69, 843–851). Similar findings have been reported following the use of an oestradiol-based protocol that also provides for a longer period of proestrus (named J-Synch; de la Mata and Bó 2012 Taurus 55, 17–23). An experiment was designed to compare the J-Synch protocol for synchronization of ovulation that allows for a prolonged proestrus with a conventional 7-day oestradiol-based protocol for FTAI in heifers. Cycling 18-month old Angus and Hereford heifers (n = 208) with a body condition score of 6 to 7 (scale of 1 to 9) were randomly allocated to 1 of 2 treatment groups. Heifers in the 7-day EB group (n = 105) received a progesterone (P4) device (DIB 0.5 g of P4, Syntex SA, Buenos Aires, Argentina) and 2 mg of oestradiol benzoate (EB, Syntex SA) on Day 0 and 500 μg of cloprostenol (PGF; Ciclase DL, Syntex SA) and 0.5 mg oestradiol cypionate (Cipiosyn, Syntex SA) on the day of DIB removal (Day 7). Heifers were also tail painted at the time of DIB removal and observed for signs of oestrus (i.e. tail paint rubbed off). Those with the tail paint rubbed off by 36 h after DIB removal were inseminated 12 h later, whereas those not showing oestrus by 36 h were FTAI at 54 h. Heifers in the J-Synch group (n = 103) received DIB and 2 mg of EB on Day 0 and PGF on the day of DIB removal (Day 6). Heifers in this group were also tail painted at DIB removal, and those with their tail paint rubbed off by 48 h were inseminated 12 h later; those not showing oestrus by 60 h received 100 μg of gonadorelin acetate (gonadotropin-releasing hormone, Gonasyn gdr, Syntex SA) and were FTAI at 72 h after DIB removal. Pregnancy was diagnosed by ultrasonography at 55 days after FTAI (Honda 101V, 5.0-MHz transducer). Data were analysed by logistic regression. Oestrus detection rate and pregnancy rate to FTAI did not differ (P > 0.1) between groups (38.8%, 40/103 and 60.3%, 38/ 63 for heifers in the J-Synch group v. 28.5%, 30/105 and 45.3%, 34/75 for those in the 7-day EB group). However, pregnancy rates to observed oestrus tended (P < 0.09) to be higher and the overall pregnancy rate was significantly higher (P < 0.01) in heifers in the J-Synch group (80.0%, 32/40 and 67.9%, 70 /103) compared with those in 7-day EB group (50%, 15/30 and 46.6%, 49/105). Furthermore, heifers within the J-Synch group that had their tail paint rubbed off by 48 h after DIB removal and were AI 12 h later (i.e. 60 h) had higher (P < 0.05) pregnancy rate than those in the same group that were FTAI. In conclusion, reducing the time of progestin device insertion and lengthening the proestrus period, as in the J-Synch protocol, results in higher pregnancy rates than with the conventional oestradiol-based protocol. Furthermore, the combination of oestrus detection and FTAI would appear to improve the pregnancy outcome even more.

16 FERTILITY RATE OBTAINED WITH A NEW INTRAVAGINAL PROGESTERONE RELEASING DEVICE DICO IN SHEEP

2011 ◽  
Vol 23 (1) ◽  
pp. 114
Author(s):  
C. García-Pintos ◽  
P. C. dos Santos-Neto ◽  
A. Menchaca
Keyword(s):  
Pregnancy Rate ◽  
Body Condition Score ◽  
Intrauterine Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Fertility Rate ◽  
Device Removal ◽  
Control Serum ◽  
Significant Difference ◽  
Intravaginal Device

A new intravaginal device DICO (Dispositivo Intravaginal Caprino Ovino, 0.3 g progesterone; Syntex, Buenos Aires, Argentina) consisting of a progesterone impregnated medical silicone was developed for oestrus synchronization in sheep and goats. The effectiveness of this device to control serum progesterone concentrations, follicular development and time of ovulation has been recently reported in ewes (Vilariño et al. 2010 Small Rumin. Res. 91, 219–224); however, fertility obtained with DICO has not been evaluated yet. The aim of this study was to determine fertility rate obtained with DICO by fixed-time cervical or intrauterine insemination in sheep. The experiment was performed on a total of 569 Corriedale multiparous ewes in breeding season, with a body condition score of 3.0 ± 0.1 (scale 0–5). Ewes received a Short-term Protocol consisting of a 6 days of treatment with DICO (n = 174), CIDR-G (0.3 g of progesterone, Pfizer, NY, USA; n = 197) or sponges with 60 mg of medroxiprogesterone acetate (MAP, Syntex; n = 198). One dose of prostaglandin F2α analogue (125 μg of cloprostenol, Cilcase DL, Syntex) and 300 IU of eCG (Novormon, Syntex) was given at the time of device withdrawal. Insemination was performed 48 or 54 h after device removal by cervical (200 millions of spermatozoa) or laparoscopic intrauterine insemination (100 millions of spermatozoa), respectively. Pregnancy diagnosis was performed by transrectal ultrasonography (5 MHz, Well D, Beijing, China) 40 days after insemination. Pregnancy rate (pregnant ewes/inseminated ewes) was compared by logistic regression, and number of fetuses/pregnant ewes was analysed by Poisson regression. Regarding pregnancy rate, a significant difference (P < 0.05) was found between cervical (39.5%, 109/276) v. intrauterine insemination (58.3%, 172/295), as well as between progesterone devices (DICO: 55.7%, 97/174; CIDR-G: 55.8%, 110/197) v. MAP sponges (37.4%, 74/198). The results are shown in Table 1. In conclusion, the current study shows that the new intravaginal device DICO is useful to obtain acceptable fertility with fixed-time insemination in sheep. When cervical insemination at 48 h or intrauterine insemination at 54 h after device removal is performed, similar or better results than CIDR-G or MAP sponges are achieved, respectively. Table 1.Results obtained with DICO, CIDR and sponges after cervical or intrauterine insemination in sheep Supported by Syntex.

scholarly journals Influence of nutritional and ovarian parameters on pregnancy rates of Nelore cows artificially inseminated at fixed time

2020 ◽  
Vol 9 (9) ◽  
pp. e907998091
Author(s):  
Maria Paula Marinho de Negreiros ◽  
Guilherme Henrique de Freitas Seugling ◽  
Ana Beatriz Marques de Almeida ◽  
Myrian Megumy Tsunokawa Hidalgo ◽  
Maria Isabel Mello Martins ◽  
...  
Keyword(s):  
Weight Gain ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Body Condition Score ◽  
Transrectal Ultrasound ◽  
Fixed Time ◽  
The Body ◽  
Antral Follicles ◽  
Pregnancy Diagnosis ◽  
Condition Score

This experiment aimed to evaluate the effect of weight gain, body condition score and antral follicle count on the fertility of cows subjected to fixed-time artificial insemination. To this study, it was used 194 Nelore, pluriparous cows, between the 30th and the 45th day of the postpartum period. The cows were weighed, was classified by the body condition score and a transrectal ultrasound was performed in the ovaries to identify the corpus luteum and for counting the antral follicles. Then, the females were submitted to a hormonal protocol to perform the artificial insemination in fixed time. Thirty days after the artificial insemination, the cows were weighed again and the pregnancy was diagnosed by transrectal ultrasound. The pregnancy rate did not interact (P>0.05) with the body score condition, nor the presence of the corpus luteum, neither with the antral follicles count. The weight gain between the artificial insemination moment and the pregnancy diagnosis showed an interaction (P = 0.02) with the pregnancy rate. The weight gain average between the FTAI and the pregnancy diagnosis was 9.0 kg. The animals that gained up to 73 kg, showed higher pregnancy rate. In conclusion, the weight gain between the artificial insemination in fixed time and the pregnancy diagnosis influenced the pregnancy rate.

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