24 EFFECT OF GnRH ON FIXED-TIMED ARTIFICIAL INSEMINATION PREGNANCY RATES OF WHITE-TAILED DEER

2010 ◽  
Vol 22 (1) ◽  
pp. 170
Author(s):  
J. Lambe ◽  
W. Forbes ◽  
B. M. Olcott ◽  
D. E. Sanders ◽  
R. A. Godke ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Transrectal Ultrasonography ◽  
Estrus Synchronization ◽  
Control Groups ◽  
Motile Cells ◽  
Timed Artificial Insemination ◽  
And Control ◽  
Uterine Body ◽  
Synchronization Protocol

During the fall 2008 breeding season in Louisiana, 2 synchronization protocols for fixed-timed artificial insemination (FTAI) in White-tailed deer were assessed. The objective was to determine if GnRH at FTAI improved pregnancy rates in White-tailed deer. White-tailed does (n = 35) with a mean body weight of 65.8 kg (range: 54.0 to 79.8 kg) and average age of 5.9 years (range: 1.5 to 9.5 years) were stratified by weight, age, and last fawning date into 2 groups. Treatment and control synchronization protocols were randomly assigned to each group. Does were synchronized with a CIDR-G device for 14 d and were then subjected to either FTAI 60 hpost-CIDR removal (control, n = 17) or 100 μg (i.m) injection of GnRH at FTAI 60 h post-CIDR removal (treated, n = 18). At insemination (AI), insemination pipette placement score (IP; 0 = at cervical os to 3 = within uterine body), mucous scores (clear/cloudy, viscous/nonviscous, or no secretions), vulva assessments (hyperemic/nonhyperemic and swollen/not swollen), and sperm progressive motility were recorded. Does were inseminated with frozen-thawed sperm (5 × 107 progressively motile cells pre-freeze) from 2 fertile bucks stratified across each treatment group. Starting 28 d following AI, intact bucks (ITB) were introduced into both groups for natural mating. Pregnancy was determined via transrectal ultrasonography 73 or 80 d postinsemination. Does, confirmed pregnant via ultrasonography, had fawns within the reported gestation range of 187 to 222 days. Placentomes were visualized and measured in AI pregnancies (range: 32.7 to 56.2 mm in length), whereas pregnancies derived from ITB presented no identifiable placentomes. However, crown-rump measurements were obtainable from ITB pregnancies (range: 13.4 to 21.7 mm). Five does were not included in the final analyses as they were either lost to predators or removed because of illness. IP (1.4 ± 0.24 v. 1.3 ± 0.30; P = 0.671), mucous classifications (3.0 ± 0.35 v. 2.5 ± 0.39; P = 0.311), vulva assessments (2.1 ± 0.29, 1.7 ± 0.23; P = 0.223), and sperm motility (1.6 ± 0.16, 1.7 ± 0.22; P = 0.829) were not different for pregnant and nonpregnant AI does, respectively. Treatment did not affect AI pregnancy rates (53 v. 27%) or fecundity rates (1.6 v. 1.3 offspring/doe) for the GnRH treated and control groups, respectively. Addition of GnRH to a 14-day estrus synchronization protocol did not result in significantly higher pregnancy rates compared with controls. More studies are needed to determine the effect of GnRH on White-tailed pregnancies following FTAI protocols. We have demonstrated that differentiating pregnancies derived from AI and ITB could be accomplished by utilizing transrectal ultrasonography as early as 73 d postinsemination in White-tailed does.

scholarly journals PSII-21 Vaginitis incidence and effects on fertility of beef females enrolled on estrus synchronization protocols using a controlled internal drug release (CIDR) device

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 242-242 ◽  
Author(s):  
Nicholas W Dias ◽  
Alvaro Sales ◽  
Claire Timlin ◽  
Stefania Pancini ◽  
John Currin ◽  
...  
Keyword(s):  
Drug Release ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Pregnancy Status ◽  
Timed Artificial Insemination ◽  
System 1 ◽  
Synchronization Protocol

Abstract Our objectives were to characterize the incidence of vaginitis caused by a controlled internal drug release (CIDR) device and its effects on fertility of beef females enrolled on estrus synchronization followed by timed-artificial insemination (TAI). A total of 1,097 mature cows and 215 heifers from 9 different locations were enrolled in the 7-d CO-Synch + CIDR synchronization protocol. At CIDR insertion, all females were inspected for any previous signs of vaginitis prior to enrollment in the experiment. As evidence of vaginitis, a CIDR score was determined at CIDR removal, and an AI plastic sheath score was also performed at TAI following a 1 to 4 system: 1=clean presenting no secretion; 2=clean presenting clear secretion; 3=presence of purulent secretion; 4=presence of purulent secretion and blood. Pregnancy status was determined by rectal ultrasonography approximately 40 days after TAI. The scores for CIDR and AI plastic sheath did not differ between cows and heifers. Of all females, 6% had a CIDR score of 1, 31% score 2, 56% score 3, and 7% score 4. Plastic sheath score was 1 for 16%, 2 for 62%, 3 for 15%, and 4 for 7% of females. Pregnancy rate to TAI differed between cows and heifers (P < 0.01), among locations (P < 0.001) and ranged from 36 to 66%; however, it was not influenced by CIDR score (P = 0.259) and plastic sheath score (P = 0.785). Pregnancy rate of females by different CIDR scores was 60%, 57%, 52%, and 46% for scores 1, 2, 3, and 4, respectively. Pregnancy rate of females by different AI plastic sheath score was 60%, 55%, 45%, and 48%, for scores 1, 2, 3, and 4, respectively. The presence of vaginitis caused by a CIDR does not influence pregnancy rates of TAI in beef females.

scholarly journals PSXIII-11 Altering length of the presynchronization period in a long-term progestin-based estrus synchronization protocol for timed artificial insemination of beef heifers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 375-376
Author(s):  
Emma R Knickmeyer ◽  
Jordan M Thomas ◽  
James William C Locke ◽  
Rachael C Bonacker ◽  
Lauren Ciernia ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Reproductive Tract ◽  
Prostaglandin F2α ◽  
Estrus Synchronization ◽  
Dominant Follicle ◽  
Estrus Detection ◽  
Timed Artificial Insemination ◽  
Split Time ◽  
Synchronization Protocol

Abstract An experiment was designed to evaluate the effect of extending the presynchronization period in a progestin-based estrus synchronization protocol. Heifers were assigned to an 18 d or 14 d CIDR® treatment (1.38 g progesterone), with prostaglandin F2α (PG; 250 mg im) administered 16 d after CIDR® removal (Day 34). Heifers at two locations (location one, n = 193; location two, n = 649) were assigned to treatment based on reproductive tract score (RTS; Scale 1–5) and weight. Estrus detection aids (Estrotect®) were applied at PG and split-time artificial insemination (STAI) was performed based on estrus expression at 66 h. Heifers that expressed estrus at 66 h were inseminated and heifers that did not express estrus were inseminated at 90 h. Only heifers that failed to express estrus by 90 h received gonadotropin-releasing hormone (GnRH; 100 µg im) at AI. At location one, blood samples were collected at PG and AI (66 h or 90 h) from all heifers to determine E2 concentrations, and transrectal ovarian ultrasound was performed to detail ovarian structures on a subset of heifers (n = 73) at both time points. The proportion of heifers expressing estrus by 66 h (60%) or in total by 90 h (84%) after PG did not differ between treatments. Pregnancy rate to STAI did not differ between treatments (P = 0.3; 52%, 14 d CIDR®-PG; 50%, 18 d CIDR®-PG), or at the end of the 60 d breeding season (P = 0.2). There were no differences between treatments in mean diameter of the dominant follicle at PG (P = 0.6) or STAI (P = 0.3), nor were any differences observed between treatments in E2 concentrations at PG (P = 0.8) or STAI (P = 0.6). These data suggest that CIDR® treatment can be extended from 14 to 18 d, providing flexibility in scheduling without compromising reproductive outcomes.

scholarly journals PSXIII-12 Evaluation of split-time artificial insemination following administration of the 7-d CO-Synch + CIDR® estrus synchronization protocol in beef heifers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 374-374
Author(s):  
Emma R Knickmeyer ◽  
Jordan M Thomas ◽  
James William C Locke ◽  
Rachael C Bonacker ◽  
Mark R Ellersieck ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Reproductive Tract ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Beef Heifers ◽  
Estrus Detection ◽  
Delayed Time ◽  
Split Time ◽  
Synchronization Protocol

Abstract Estrous response and pregnancy rates resulting from fixed-time (FTAI) or split-time (STAI) artificial insemination were compared among heifers following treatment with the 7-d CO-Synch + CIDR® (controlled internal drug release;1.38g progesterone) protocol. Heifers (n = 456) were assigned to balanced treatments based on weight and reproductive tract score (RTS; Scale 1–5). Gonadotropin-releasing hormone (GnRH; 100 µg gonadorelin acetate) was administered coincident with CIDR® insertion. Progesterone inserts were removed after 7 d, and PG (250 mg im cloprostenol sodium) was administered at CIDR® removal. Estrus detection aids (Estrotect®) were applied at the time PG was administered. Estrous status was recorded at FTAI or STAI, and estrus was defined as removal of ≥ 50% of the grey coating from the Estrotect® patch. Heifers assigned to the FTAI treatment received GnRH and were artificially inseminated 54 h after PG administration. In the STAI treatment, only heifers that expressed estrus prior to 54 h were artificially inseminated at that time. For heifers failing to express estrus, AI was postponed 24 h and only those heifers that failed to exhibit estrus by the delayed time (78 h) received GnRH concurrent with AI. Estrous response prior to the standard time of FTAI did not differ between treatments (P = 0.3). Total estrous response was increased (P < 0.001) among heifers assigned to STAI (74%, STAI; 47%, FTAI); however, pregnancy rates resulting from AI were similar between treatments (48%, STAI; 46%, FTAI; P = 0.4), as were pregnancy rates at the end of a 60 d breeding season (P = 0.6). In summary, STAI resulted in a greater estrous response following treatment with the 7-d CO-Synch + CIDR® protocol; however, this did not result in an increase in pregnancy rate compared to heifers that received FTAI.

scholarly journals 7 HETEROSPERMIC INSEMINATION AT TWO SPERM CONCENTRATIONS IN TIMED AI: CASA SEMEN PARAMETERS AND PREGNANCY RATES

2005 ◽  
Vol 17 (2) ◽  
pp. 154 ◽  
Author(s):  
A. Albrecht ◽  
R. Cavia ◽  
G. Larraburu ◽  
F. Garcia Migliaro ◽  
G. Brogliatti
Keyword(s):  
Artificial Insemination ◽  
Semen Analysis ◽  
Pregnancy Rates ◽  
Semen Parameters ◽  
Computer Assisted ◽  
High Concentration ◽  
Bull Semen ◽  
Motile Cells ◽  
Timed Artificial Insemination ◽  
Motility Parameters

The latest entry in the field of semen evaluation is computer assisted semen analysis (CASA). Heterospermic insemination has been used to increase pregnancy rates from low fertile bulls. The objective of this study was to evaluate, with the aid of CASA, heterospermic semen characteristics and pregnancy rates using different concentrations of bull semen in a timed artificial insemination protocol. Semen was collected from two bulls of known fertility by artificial vagina and all CASA motility parameters were evaluated individually and combined. Straws were filled using a semi-defined semen extender (Andromed, Minitüb, Tiefenbach, Germany) as follows: single bull A and single bull B (12 × 106 of progressive motile cells after thawing); Mixed bull semen: A + B (12 × 106 of progressive motile cells after thawing) and Supermix bull semen: A + B (28 × 106 of progressive motile cells after thawing). All cows received a P4 intravaginal device (DIB, Syntex, Argentina) and 2 mg of EB i.m. (Syntex) on Day 0, 500 mg cloprostenol (Estroplan, Syntex) at the time of DIB removal (Day 8), and 1 mg EB i.m. on Day 9. Fixed-time insemination (FTAI) was performed at 52 to 56 h after DIB removal. A total of 249 cows were randomly allocated to be inseminated with bulls A and B (n = 76), with Mixed A + B (n = 87), and with Supermixed A+B at a high concentration (n = 86) by a single inseminator. Pregnancy rates were evaluated at 38 days after insemination by transrectal ultrasonography. Means and standard deviations or each characteristic were calculated, compared, and statistically analyzed. The following sperm motility parameters were determined with the Ceros 12.1 sperm analyzer (Hamilton Thorne Biosciences, Inc., Beverly, MA, USA) on at least 1000 spermatozoa: velocity average path (VAP), velocity straight line (VSL), curvilinear velocity (VCL), amplitude lateral head (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN), and percentage of rapid or static cells. There were no significant differences (P > 0.05) in VAP, VSL, VCL, ALH, STR, or LIN. There was a numerically higher percentage of rapid cells in the Supermix semen. Pregnancy rate from bulls A and B was 61% and from Mixed A + B 60%, while that from Supermixed A + B was 69%. Results from the analysis indicate that semen concentration is an important element to be considered in a timed artificial insemination program. Numerically higher pregnancy rates were obtained with double semen concentration in the straw. More research is required to evaluate the interaction between different breeds within a timed artificial insemination program. This research was supported by Centro Genético Bovino de EOLIA sa and Syntex sa Argentina.

160 PREGNANCY RATES AFTER USING THE CIDR-G DEVICE AND FIXED-TIMED ARTIFICIAL INSEMINATION IN WHITE-TAIL DEER

2008 ◽  
Vol 20 (1) ◽  
pp. 160
Author(s):  
G. T. Gentry Jr ◽  
J. Saenz ◽  
W. Forbes ◽  
K. Whitehead ◽  
D. Sanders ◽  
...  
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Gestation Length ◽  
Treatment Groups ◽  
Timed Artificial Insemination ◽  
Synchronization Protocol

Two synchronization protocols for fixed-timed AI (FTAI) in White-tail deer were evaluated over a 2-year period. InYear 1, White-tail does (n = 38), with a mean body weight of 55 kg and mean age of 2.4 years, were stratified by weight, age, and last fawning date randomly across 2 estrous cycle synchronization treatment groups. Does received either a CIDR-G device for 14 days (CIDR 14) and underwent AI 60 h post-CIDR removal, or received a CIDR-G device for 7 days (CIDR 7) with 1 mg of estradiol benzoate (i.m.) at CIDR insertion (Day 0), 25 mg of prostaglandin F2α (PGF2α; Dinoprost) on Day 6 and 1 mg of estradiol benzoate (i.m.) on Day 7 with AI occurring 52 h post-CIDR removal. In Year 2, White-tail does (n = 36), with a mean body weight of 60 kg and mean age of 3.9 years, were stratified as described for Year 1 and allotted to 2 treatments. Does received a CIDR-G device for 14 days and were inseminated 60 h post-CIDR removal (Treatment A) or were synchronized in the same way as does inTreatment A but also received 200 IU (i.m.) of eCG (Sigma, St. Louis, MO) at CIDR removal (Treatment B). All does received 1 mg of Domosedan (i.v.) before initiating the AI procedure. In both years, electroejaculated semen was collected from a single buck and frozen for AI. In both years, clean-up bucks were introduced into the pen with does for natural cover at no less than 14 days following AI. A gestation length of 195 � 7 days was used to determine whether the fawn(s) resulted from AI or from natural mating. In Year 1, FTAI pregnancy rates were not different between the CIDR 14 (56%) and CIDR 7 (24%) treatment groups. Likewise, pregnancy rates were not different between CIDR 14 FTAI (56%) and clean-up bucks (75%). However, the FTAI pregnancy rate was lower (P < 0.001) for the CIDR 7 treatment group (24%) compared with the clean-up bucks (100%). Treatments did not affect fecundity rates; however, those females pregnant from FTAI had lower (P < 0.001) fecundity rates compared with those females pregnant from natural cover (1.2 v. 1.9 fawns/doe). In Year 2, treatment did not affect FTAI pregnancy rates across treatments (33% for Treatment A and 55% Treatment B); however, fewer (P = 0.024) does in Treatment A were pregnant following FTAI (33%) compared with those pregnant from clean-up bucks (75%). There was no difference in FTAI fecundity rates across treatment groups (1.3 v. 1.7 for Treatments A and B, respectively) or between FTAI treatments and natural cover (1.3, 1.7, and 1.5 for Treatments A, B, and clean-up bucks, respectively). These results indicate that the use of a 14-day CIDR synchronization protocol with FTAI occurring 60 h after CIDR removal yields acceptable pregnancy rates of White-tail does following transcervical AI.

scholarly journals Timed Artificial Insemination: Pregnancy Rates in Sokoto Gudali Cattle Treated with Prostaglandin F2 Alpha at a Private Dairy Farm in Nigeria.

2019 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Olufisayo Oluwadamilare Leigh ◽  
Lukman Mustapha ◽  
Agbugba Chinyere Linda ◽  
Adaeze Ezenwanyi Ibiam
Keyword(s):  
Artificial Insemination ◽  
Day Treatment ◽  
Single Agent ◽  
Dairy Farm ◽  
Pregnancy Rates ◽  
Future Application ◽  
Estrus Synchronization ◽  
Prostaglandin F2 Alpha ◽  
Frozen Semen ◽  
Timed Artificial Insemination

Pregnancy rates (PR) resulting from timed artificial insemination (TAI) with frozen semen were investigated in 192 Sokoto Gudali (SG) cattle following estrus synchronization under 11- and 12- day treatment intervals with prostaglandin F2 alpha (Estrumate®). Two-point inseminations at 60 h and 72 h post Estrumate® treatments were used. All cattle were randomly allocated to 2 groups i.e. A and B, such that each sub-group of A1, A2, B1 and B2 had sixteen (16) cows and thirty two (32) heifers. Furthermore, cattle in Groups A and B were administered double Estrumate® injections at 11- and 12-day intervals, respectively. Cattle in sub-groups A1 and B1 were inseminated at 60 h post second Estrumate® injection while A2 and B2 were inseminated at 72 h. Pregnancy was diagnosed using the 60-day non return to estrus and confirmed via rectal palpation at 90 days post insemination. The results showed the following PR: 33%, 83%, 33% and 33% for cattle in sub-groups A1, A2, B1 and B2, respectively. It is concluded therefore that timed artificial insemination at 72 h in SG cattle treated with double Estrumate® injections at 11-day interval produced the best pregnancy rate. These findings raises hope on the future application of TAI in breeding the SG cattle with  prostaglandin F2 alpha as single agent of estrus synchronization.      

scholarly journals Effect of melatonin on follicular development parameters in a fixed-timed artificial insemination programme in water buffalo

2021 ◽  
Vol 52 (6) ◽  
Author(s):  
Jesus Alfredo Berdugo ◽  
Jose Luis Konrad ◽  
Roberto Yuponi ◽  
Alexis Bandeo ◽  
Ramon Camelino ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Water Buffalo ◽  
Follicular Development ◽  
Ovarian Response ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Reproductive Season ◽  
Frozen Semen ◽  
Timed Artificial Insemination ◽  
And Control

It is well known that buffaloes have seasonal anoestrus and that the use of melatonin reduces the effects of season in reproduction, articularly among sheep and goats. This study evaluates the use of melatonin on water buffaloes to increase pregnancy rates in a fixed-time insemination programme. The study was conducted on an Argentinian buffalo herd, located in the Corrientes Province, during an unfavourable reproductive season. Artificial insemination cycles were performed in September and December 2018. Sixty animals were selected, and melatonin was administered to 29 females at a dose of 18 mg/50 kg. Ten days after the melatonin implant, ovulation synchronization was started. Animals were inseminated with frozen semen of a single bull. Pregnancy was evaluated by ultrasound 35 days after insemination. The pregnancy rate of the two repetitions was 34.62% for September and 6.4% for December (P=0.007). No significant differences were found in the parameters associated with follicular development and formation of the corpus luteum. Analysis of the effects of melatonin on the two replicates showed that there was no statistically significant effect of ovarian response regarding the analysed follicular development parameters. There was also no effect on pregnancy rates (17.85% vs. 20.68%; P=0.15), or on follicular development for treatment and control. In conclusion, this study was not able to demonstrate an effect of melatonin on the ovarian response of buffaloes as reported by other authors. Other factors, such as environmental conditions of subtropical areas and species specificities, may have a more significant effect on buffalo endocrinology. These factors should be evaluated to improve the results of fixed-timed artificial insemination programmes.

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