296 EFFECT OF GnRH AND ESTRADIOL BENZOATE INJECTION ON SUPEROVULATORY RESPONSE IN EARLY POSTPARTUM SUCKLING JAPANESE BLACK COWS

2009 ◽  
Vol 21 (1) ◽  
pp. 245
Author(s):  
K. Oshima ◽  
Y. Ochiai ◽  
T. Kojima ◽  
N. Yamamoto
Keyword(s):  
Embryo Quality ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Control Groups ◽  
Treatment Groups ◽  
Early Postpartum ◽  
Uterine Flushing ◽  
Days Open ◽  
And Control ◽  
Estrous Behavior

The objective of this study was to investigate the effect of GnRH and EB injection on the SOV response in early postpartum suckling Japanese Black cows. Forty-eight cows were included in this study. All cows received a controlled internal-drug-releasing device containing 1.9 g progesterone (CIDR; Easi-Breed; InterAg, Hamilton, New Zealand) at 40 days postpartum, and were subsequently superstimulated with a total dose of 20 armour units FSH (Antrin 40; Kawasaki-Mitaka, Kanagawa, Japan) twice daily, with gradually decreasing doses from day 45 till day 47. CIDR was withdrawn with 0.75 mg of cloprostenol (Estrumate; Nagase Medicals, Itami, Japan) injection at day 47. After FSH treatment, the cows were assigned to 3 groups: (1) 100 μg GnRH (Conceral; Nagase Medicals) injection at day 49 (GnRH, n = 19), (2) 2 mg EB (Kawasaki-Mitaka) injection at day 48 (EB, n = 15), and (3) control (n = 14). AI was carried out twice based on the estrous behavior of the cows, which was examined several times daily. Embryos were nonsurgically collected 7 to 8 days after estrus. The ovaries were examined by ultrasonography, and the number of CL and unovulated follicles (UF) were counted. After uterine flushing, the cows were re-employed for reproductive purposes. The intervals to first estrus after flushing and days open were examined. Blood samples were collected temporally, and concentrations of progesterone (P4) and estradiol (E2) were measured. The ovaries were examined by ultrasonography and the number of CL, small follicles (<5 mm in diameter), medium follicles (between 5 and 10 mm in diameter), and large follicles (>10 mm in diameter) were counted temporally. Data were analyzed by GLM and Dunnett’s T3 test. There were no significant differences between both treatment groups and the control group in the number of CL (15.2 ± 6.8, 18.9 ± 9.4, and 14.9 ± 8.9 in the GnRH, EB, and control groups, respectively; mean ± SD), UF (5.3 ± 3.2, 4.7 ± 3.2, and 7.2 ± 9.5, respectively), recovered ova or embryos (11.4 ± 8.1, 11.1 ± 8.9, and 11.1 ± 9.1, respectively), transferable embryos (6.8 ± 5.3, 7.5 ± 7.6, 7.2 ± 6.7, respectively), and freezable embryos (6.3 ± 5.6, 6.6 ± 7.9, and 5.7 ± 6.1, respectively). Furthermore, there were no significant differences in the intervals to first estrus after flushing and days open (12.7 ± 13.6 and 90.0 ± 23.1 in the GnRH, 8.6 ± 5.3 and 79.1 ± 20.1 in the EB, and 9.4 ± 6.0 and 88.5 ± 33.8 in the control groups, respectively). Changes in the P4 level were similar among the 3 groups. The E2 level of the EB group at 15 h after EB injection was significantly higher (P < 0.05) than that in the other groups (19.0 ± 15.7, 49.4 ± 22.8, and 8.0 ± 5.7 pg mL–1 in the GnRH, EB, and control groups, respectively). The number of small follicles in the control group (17.0 ± 12.2) was significantly greater (P < 0.05) than that in the GnRH group (7.4 ± 7.2) at the first AI. These results suggest that EB and GnRH injection after SOV treatment in early postpartum Japanese Black cows does not affect the SOV response or embryo quality.

Efficacy of Ovsynch and Ovsynch Plus Protocol for Improvement of Fertility in Postpartum Sahiwal Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
J. P. Lakher ◽  
M. K. Awasthi ◽  
J. R. Khan ◽  
M. R. Poyam
Keyword(s):  
Fixed Time ◽  
Conception Rate ◽  
Control Group ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I ◽  
Early Postpartum ◽  
The Status ◽  
Sahiwal Cows ◽  
And Control

The present study was conducted to investigate the efficacy of Ovsynch and Ovsynch plus protocol in postpartum (day 60) Sahiwal cows (n=18). Animals were randomly divided into three equal groups, viz., Ovsynch group Ovsynch plus group and Control group. Animals of group I (n = 6) were treated with traditional Ovsynch protocol. The animals (n = 6) of group II were treated with Ovsynch plus protocol which consisted of an initial intramuscular injection of eCG (Folligon) @ 250 IU on day 60 postpartum followed 3 days later by GPG (Ovsynch) protocol. In group-III Control, no treatment was given to animals (n = 6). Treated animals were inseminated at a fixed time between 14 and 20 hrs after second GnRH injection, irrespective of estrus detection. Blood samples were collected from each animal on days 50 and 60 postpartum to determine the status of cyclicity in animals based on serum concentrations of progesterone (P4). A third blood sample was collected on the day of prostaglandin treatment to determine the response of first GnRH injection. Four animals each were cyclic, and two were acyclic in both treatment groups. Four animals each responded to first GnRH treatment in both treatment groups. Similarly, two animals each got conceived giving conception rate of 50% (2/4) in each treatment. In the control group, one out of 6 animals got conceived yielding 16.66 % conception rate (1/6) during the study period. It may be thus concluded that Ovsynch and Ovsynch plus protocol may be used during the early postpartum period to improve the reproductive efficiency in postpartum Sahiwal cows.

scholarly journals Effects of Intrauterine Infusion of a Chitosan Solution on Recovery and Subsequent Reproductive Performance of Early Postpartum Dairy Cows with Endometritis: A Pilot Field Trial

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 197
Author(s):  
Hiroaki Okawa ◽  
Missaka M.P. Wijayagunawardane ◽  
Peter L.A.M. Vos ◽  
Osamu Yamato ◽  
Masayasu Taniguchi ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Reproductive Performance ◽  
Polymorphonuclear Leukocytes ◽  
Prostaglandin F2α ◽  
Chitosan Solution ◽  
Control Group ◽  
Control Groups ◽  
Early Postpartum Period ◽  
Early Postpartum ◽  
And Control

This study investigated the efficacy of intrauterine infusion of a chitosan solution (CHT) on uterine recovery in early postpartum dairy cows with or without endometritis, and their subsequent reproductive performance. In Experiment 1, cows with endometritis at 3 weeks postpartum were administered CHT (n = 5) and prostaglandin F2α (PGF2α) (n = 4). Untreated cows (n = 7) served as the control group. In Experiment 2, 18 cows with a normally recovered uterus at the fresh cow check (mean, 35 days postpartum) were assigned to the CHT (n = 10) and control (n = 8) groups, and intrauterine infusion was conducted in the CHT group. Overall, in Experiment 1, the percentage of polymorphonuclear leukocytes significantly declined in the CHT group (32.3 ± 10.2 to 5.5 ± 2.4, p < 0.05) from week 3 to week 5, but no decline occurred in the PGF2α and control groups. In Experiment 2, the CHT and control groups showed no significant differences in reproductive parameters, suggesting the absence of adverse effects of CHT on fertility. These results suggest that intrauterine infusion of CHT in the early postpartum period effectively accelerates uterine recovery from endometritis and might be a suitable replacement for PGF2α administration.

scholarly journals Pengaruh Terapi AIUEO terhadap Kemampuan Bicara Pasien Stroke yang Mengalami Afasia Motorik

2019 ◽  
Vol 1 (2) ◽  
pp. 226-235
Author(s):  
Afnijar Wahyu ◽  
Liza Wati ◽  
Murad Fajri
Keyword(s):  
Wilcoxon Test ◽  
Control Group ◽  
Stroke Patients ◽  
Control Groups ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Motor Aphasia ◽  
A Value ◽  
And Control

The purpose of this study was to determine the effect of AIUEO therapy on the speech ability of stroke patients who have motor aphasia in Raja Ahmad Thabib Hospital Tanjungpinang. The research design used was quasi experiment with the Nonequivalent Control Group Design approach to 9 respondents who were divided into 9 treatment groups and 9 control groups. The results showed that there were significant differences in the functional ability of communication between the control and treatment groups with a value of p <0.05 (p = 0.007 at a = 0.05) using the Wilcoxon Test statistical test. Conclusion, the influence of AIUEO therapy on the speech ability of stroke patients with motor aphasia in the treatment and control groups at Ahmad Thabib Hospital Tanjungpinang.   Keywords: Speech Ability, Motor Aphasia Stroke, AIUEO Therapy

Effect of biochemical profiles and reproductive performance during internal parasites in Goat and Sheep

2017 ◽  
Vol 2 (01) ◽  
pp. 103-109
Author(s):  
Dinesh Mahto
Keyword(s):  
Control Group ◽  
Control Groups ◽  
Treatment Groups ◽  
Group I ◽  
Internal Parasites ◽  
Mineral Mixture ◽  
Biochemical Profiles ◽  
Group Ii ◽  
Minimum Interval ◽  
And Control

Gastrointestinal parasitic infected ewes and does were kept almost parasite free for about 3 months using Albendazole alone or supplemented with mineral mixture. The Hb (g/dl) level ranged from 8.83±0.08 to 8.96±0.03 in ewes and does was 8.78±0.02 to 8.95±0.24 before treatment. The level of Hb increased significantly (P less than 0.01) in both the treatment groups after treatment on day 20.The Serum level of Ca, P, Cu and Zn in ewes and does increased significantly after treatment on day 20 in both the treatment groups as comparison to control group. Although they did not vary significantly among themselves except the level of Cu which was significantly (P less than 0.01) higher in group II than the group I in ewes and does. The level of Zn was higher in group I than group II in does. The minimum interval from treatment to onset of estrus were recorded in group II , 41 days in ewes and 43 days in does which was followed by group I 44 days in ewes and 46 days in does and control group 55 days in ewes and 52 days in does. The average required number of services per conception in does were 1.33, 1.00 and 2.00 for I, II and control groups, respectively. The corresponding values in ewes were 1.67, 1.17 and 2.17, respectively. The pregnancy rates in the three groups were 66.66, 83.33 and 33.33%, respectively in case of does. The corresponding values in ewes were 50.00, 66.66 and 33.36%, respectively.

Efficacy of Imagery and Meditation Techniques in Treating Psoriasis

1989 ◽  
Vol 8 (1) ◽  
pp. 25-38 ◽  
Author(s):  
Louise Gaston ◽  
Jean-Charles Crombez ◽  
Jacques Joly ◽  
Sheilagh Hodgins ◽  
Marc Dumont
Keyword(s):  
Time Series ◽  
Multivariate Regression ◽  
Multivariate Regression Analysis ◽  
Control Group ◽  
Control Groups ◽  
Treatment Groups ◽  
Series Design ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Using a dismantled time-series design, eighteen subjects were randomly assigned to three groups: meditation ( N = 5); meditation and imagery ( N = 4); waiting list ( N = 5). Treatment lasted twelve weeks, while pre- and post-baseline periods were of four weeks. An assessment control group control was also used during baseline periods ( N = 4). The severity of psoriasis symptoms was reliably rated by dermatologists. While controlling for extraneous factors influencing psoriasis symptoms, a time-series multivariate regression analysis revealed a significant difference between the mean psoriasis ratings of treatment and control groups after treatment (partial r = .30, p < .01). No additional impact was associated with the use of the imagery technique (partial r = − .06, p > .05). Four subjects in the treatment groups, two in each condition, succeeded in clinically improving their psoriasis symptoms, and none deteriorated. The practice of mediation may be clinically effective for some patients in reducing their psoriasis symptoms.

Comparison of Students Taught Basketball Skills Using Mastery and Nonmastery Learning Methods

1992 ◽  
Vol 11 (3) ◽  
pp. 235-247 ◽  
Author(s):  
Connie L. Blakemore ◽  
H. Gill Hilton ◽  
Joyce M. Harrison ◽  
Tracy L. Pellett ◽  
James Gresh
Keyword(s):  
Instructional Strategy ◽  
Mastery Learning ◽  
Control Group ◽  
Psychomotor Skills ◽  
Control Groups ◽  
Treatment Groups ◽  
Competitive Game ◽  
Game Situation ◽  
Significant Difference ◽  
And Control

Mastery learning is an instructional strategy that embraces the philosophy that almost any student can learn what is being taught given sufficient time and help. Through Bloom’s group-based, teacher-paced model, 71 seventh-grade boys were taught basketball skills. Students in two treatment groups (mastery and nonmastery) and a control group were compared on the performance of psychomotor skills in isolation and in a competitive game situation before, midway through, and following their training. Students in the mastery group were not taught new skills until 80% had mastered the present skills. The mastery group performed significantly better on isolated skills than did the nonmastery and control groups. There was no significant difference between groups in the performance of skills in a competitive game situation.

scholarly journals Estrus Induction and Fertility Response following Ovsynch Alone and Ovsynch plus PRID Protocol in Postpartum Anestrus Surti buffaloes

2017 ◽  
Vol 12 (4) ◽  
Author(s):  
C. T. Khasatiya ◽  
H. R. Savani ◽  
Swati Saxena ◽  
M. D. Patel
Keyword(s):  
Artificial Insemination ◽  
Fixed Time ◽  
Conception Rate ◽  
Control Group ◽  
Control Groups ◽  
Treatment Groups ◽  
The Mean ◽  
And Control

The study was undertaken to find out the estrus induction and conception rate in 18 postpartum anestrus Surti buffaloes divided equally into three groups and treated with Ovsynch protocol alone and in combination with PRID, keeping one group as control. The use of Ovsynch alone and Ovsynch + PRID protocols with fixed-time artificial insemination resulted in 100% estrus induction with 66.66% and 50.00% conception rate at induced estrus, respectively, and the overall conception rate was 83.33% in both the groups. In untreated anestrus control (n=6), only four (66.66%) buffaloes exhibited spontaneous estrus within 120 days of follow-up and conceived giving the first service and overall conception rate as 75.00% and 66.66%, respectively. The mean number of services per conception did not differ significantly among the treatment and control groups. The service periods of buffaloes under both the treatment groups were significantly (p less than 0.05) shorter (57.00±2.81 and 61.20±3.11 days) than the control group (104.00±20.22 days). Thus, the Ovsynch and Ovsynch + PRID protocol can be successfully used to induce fertile estrus in postpartum anestrus buffaloes.

scholarly journals Comparison of Oocyte and Embryo Quality in Women With Polycystic Ovary Syndrome and the Control Group Candidate for In Vitro Fertilization and Intracytoplasmic Sperm Injection

2020 ◽  
Vol 9 (3) ◽  
pp. 164-170
Author(s):  
Malihe Afiat ◽  
Nayere Khadem ◽  
Elnaz Nayeri ◽  
Roya Jalali ◽  
Saeed Akhlaghi ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Polycystic Ovary Syndrome ◽  
Embryo Quality ◽  
Polycystic Ovary ◽  
Control Group ◽  
Control Groups ◽  
Ovary Syndrome ◽  
Vitro Fertilization ◽  
And Control

Objectives: Polycystic ovary syndrome (PCOS) is the most common cause of female infertility. The aim of this study was to compare the oocyte and embryo quality between the PCOS women with the control group candidate for in vitro fertilization/ intracytoplasmic sperm injection (IVF/ICSI). Materials and Methods: The present study was designed at the Infertility Research Center of Milad in the prospective cohort format and was carried out on 100 cases of infertile women with confirmed PCOS (case group) and the male factor (control group) as the first IVF cycle candidates. Both groups underwent the ovary stimulation cycle and ICSI under the standard antagonist protocol. The collected data were then processed and analyzed using the SPSS software, version 16. Results: The average age of study cases was 35±3, and oocyte necrosis was the underlying pathological factor in both groups (28% and 26% in the PCOS and control groups, respectively). In addition, most embryones belonged to either grade 1 or 2 or were 8-cell embryos. Furthermore, the highest number of transferred embryos among the patients was related to the 8-cell and grade 1. The occurrences of biochemical pregnancy in the PCOS and control groups were up to 31.91% and 22%, respectively, leading to 72.73% and 60% childbirth in cases of both groups. Finally, there were no significant differences observed with respect to the quality and the quantity of the embryones, the oocyte, the transferred embryo, the germinal vesicle oocytes, and the rate of pregnancy among the two groups (P>0.05). Conclusions: According to the results of the present study, no differences were found concerning the oocyte quality, embryo, and the pregnancy rate between PCOS cases and any other patients requiring ICSI. Therefore, such cases can similarly benefit from ICSI methods as well.

scholarly journals Comparison of Nitroglycerin versus Lignocaine Spray to Attenuate Haemodynamic Changes in Elective Surgical Patients Undergoing Direct Laryngoscopy and Endotracheal Intubation: A prospective randomised study

2019 ◽  
Vol 19 (4) ◽  
pp. 316
Author(s):  
Rohit K. Varshney ◽  
Mukesh K. Prasad ◽  
Megha Garg
Keyword(s):  
Blood Pressure ◽  
Endotracheal Intubation ◽  
Direct Laryngoscopy ◽  
Pressor Response ◽  
Surgical Patients ◽  
Rate Pressure Product ◽  
Control Group ◽  
Control Groups ◽  
Treatment Groups ◽  
And Control

Objectives: This study aimed to compare the effects of nitroglycerin (NTG) versus lignocaine spray in blunting the pressor response during direct laryngoscopy and endotracheal intubation. Methods: This study was conducted between January and June 2018 in the Department of Anesthesiology, Teerthankar Mahaveer Medical College, Moradabad, India. A total of 90 elective surgical patients of American Society of Anesthesiologists physical status grades I or II were divided into three groups, comprising two treatment groups and one control group. Patients in the treatment groups received either one puff (1.5 mg/kg) of lignocaine 10% spray or one puff (400 μg) of NTG spray in the oropharynx one minute prior to the induction of anaesthesia. Haemodynamic variables and mean rate pressure product at baseline and one, two, three, four and five minutes post-induction were compared. Results: There was a significant reduction in mean heart rate at 3–5 minutes in both treatment groups compared to the control group (P <0.050), as well as lower increases in mean arterial pressure at 1–3 minutes (P <0.050). However, at 2–4 minutes, there was a significantly greater decrease in mean systolic blood pressure in the NTG group compared to both the lignocaine and control groups (P <0.050). Moreover, a greater decrease in mean rate pressure product response at 1–5 minutes was observed in the NTG group compared to the lignocaine and control groups (P = 0.001). Conclusion: The NTG spray was more effective than lignocaine in attenuating blood pressure increases and rate pressure product during elective laryngoscopy and intubation.Keywords: Endotracheal Anesthesia; Intubation; Laryngoscopy; Lignocaine; Nitroglycerin; Comparative Effectiveness Research; India.

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

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