294 SUPERSTIMULATION IN THE FIRST FOLLICULAR WAVE, WITHOUT THE USE OF ESTRADIOL IN BONSMARA CATTLE

2008 ◽  
Vol 20 (1) ◽  
pp. 226 ◽  
Author(s):  
D. Carballo Guerrero ◽  
A. Tribulo ◽  
R. Tribulo ◽  
H. Tribulo ◽  
G. A. Bo
Keyword(s):  
Animal Health ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Wave Group ◽  
Treatment Groups ◽  
Follicular Wave ◽  
Group Data ◽  
Mate Removal ◽  
On Farm ◽  
Experimental Group

Protocols that control follicular wave emergence and ovulation have had a great impact on the application of commercial on-farm embryo transfer because they permit the initiation of superstimulatory treatments at a self-appointed time. However, the most common approach for the synchronization of follicular wave emergence for superstimulation involves the use of estradiol or its esters that are not commercially available in many countries. Therefore, an experiment was designed to evaluate a protocol in which the superstimulation treatment began at the time of emergence of the first follicular wave without the use of estradiol. Bonsmara donors (29 cows and 41 heifers) were randomly allocated to one of two treatment groups. Donor animals in the experimental group (first wave group) received an intravaginal progesterone releasing device (Cue-Mate, Bioniche Animal Health, ON, Canada) along with PGF (0.150 mg D + cloprostenol, Bioprost-D, Biotay, Argentina) at random stages of the estrous cycle. Cue-Mates were removed 10.5 d later and a second PGF was administered at the same time, followed by GnRH (0.050 mg Lecirelina, Biosin-OV, Biotay, Argentina) 36 h later. Ovulation was expected to occur within 30 h after GnRH (day 0). On day 0 (36 h after gonadotropin-releasing hormone) donors received a new Cue-Mate, and superstimulation treatment was initiated with a total dose of 200 to 260 mg (heifers) or 320 mg (cows) NIH-FSH-P1of Folltropin-V in twice daily decreasing doses over 5 d. The PGF was administered with the last two Folltropin-V injections, and Cue-Mate devices were removed with the last Folltropin-V injection. All donors received 12.5 mg pLH (Lutropin-V, Bioniche Animal Health) 24 h after Cue-Mate removal and were AI 12 and 24 h later. Embryos were collected 7 d after pLH treatment. Donors in the Control group received a Cue-Mate and 2 mg of estradiol benzoate (EB; Bioestradiol, Biotay) and 50 mg of progesterone (Lab. Rio de Janeiro, Argentina), and superstimulation treatments were initiated 4 d later with the same dosages used in the first wave group. The PGF administration, Cue-Mate removal, AI, and embryo collections were done as those in the first wave group. Data were analyzed by ANOVA, and results are shown in Table 1. It was not possible to pass the cervix with the collection catheter in two heifers in the control group, and they were excluded from the analysis. There were no significant effects of donor category (cows v. heifers) or treatment on superovulatory response and embryo quality (P > 0.20). In conlusion, superstimulation on a synchronized first follicular wave is as efficacious as superstimulation following synchronization of follicle wave emergence with estradiol benzoate in Bonsmara cattle. Table 1. Superovulatory response (means ± SEM) in Bonsmara cows and heifers treated with Folltropin-V during the first follicular wave or 4 d after estradiol administration Bioniche Animal Health, Belleville, ON, Canada.

404 SUPEROVULATORY RESPONSE IN BEEF DONORS TREATED DURING THE FIRST FOLLICULAR WAVE OR FOUR DAYS AFTER PROGESTERONE AND ESTRADIOL ADMINISTRATION

2010 ◽  
Vol 22 (1) ◽  
pp. 358 ◽  
Author(s):  
D. Carballo Guerrero ◽  
A. Tríbulo ◽  
R. Tríbulo ◽  
H. Tríbulo ◽  
G. A. Bó
Keyword(s):  
Estrous Cycle ◽  
Animal Health ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Wave Groups ◽  
Follicular Wave ◽  
Estrus Detection ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Although we have previously shown that ovarian superstimulation during the first follicular wave resulted in a successful response (Carballo Guerrero D et al. 2009 Reprod. Fertil. 21, 242), the current protocol needs to be optimized in order to be used in the field. Therefore, an experiment was designed to simplify this treatment and to compare it with the traditional superstimulation protocol using progesterone and estradiol. Simmental cows (n = 14) were subjected to 3 superstimulation treatments (2 first wave groups and 1 control group) in a crossover design (i.e. all cows received the 3 treatments and all treatments were represented on each collection day). Cows in Group 1 received a progesterone-releasing device (Cue-Mate®, Bioniche Animal Health, Belleville, Ontario, Canada) along with 0.150 mg of D + cloprostenol (PGF; Bioprost-D®, Biotay, Buenos Aires, Argentina) at random stages of the estrous cycle. A second PGF was injected 5 days after Cue-Mate® insertion, followed by GnRH (0.050 mg of lecirelin; Biosin-OV®, Biotay) 36 h later (i.e. 7 days after Cue-Mate® insertion). Based on previous studies, ovulation was expected to occur 30 to 36 h later. Therefore, superstimulation treatments were initiated 36 h after GnRH (Day 0), with a total dose of 400 mg NIH-FSH-P1 of Folltropin®-V (Bioniche Animal Health) in twice-daily decreasing doses over 4 days. Prostaglandin was administered with the last 2 Folltropin®-V injections and Cue-Mate® devices were removed with the last Folltropin®-V injection. Cows received 12.5 mg of porcine LH (Lutropin®-V, Bioniche Animal Health) 24 h after Cue-Mate® removal and were AI 12 and 24 h later. Ova/embryos were collected 7 days after porcine LH and evaluated following IETS recommendations. Cows in Group 2 were treated similarly to those in the Group 1, except they did not receive the second PGF injection 5 days after Cue-Mate® insertion (thus eliminating the need to handle animals on that day). Finally, cows in Group 3 [estradiol benzoate (EB)+P4 control group] received a Cue-Mate® plus 2.5 mg of EB (Bioestradiol®, Biotay) and 50 mg of progesterone (P4; Lab., Rio de Janeiro, Argentina) at random stages of their estrous cycle. Superstimulation treatments were initiated 4 days later (Day 0) following the same protocol used in Group 1. Data were transformed to square root and analyzed by ANOVA. Mean (± SEM) numbers of ova/embryos collected, fertilized ova, and transferable embryos did not differ among groups (12.9 ± 2.0, 9.8 ± 1.7, and 6.6 ± 1.2; 11.5 ± 1.7, 9.3 ± 1.5, and 7.7 ± 1.6; and 14.5 ± 2.8, 9.4 ± 2.3, and 6.8 ± 1.7 for Groups 1, 2, and 3, respectively). In conclusion, data demonstrated that superstimulation during the first follicular wave can be successfully used in groups of randomly cycling donors without the need for estrus detection or estradiol to synchronize follicular wave emergence. The protocol is easy to follow and embryo production is comparable to that of the estradiol and progesterone protocol.

scholarly journals 20 EFFECTS OF DOSE OF ESTRADIOL BENZOATE AND PROGESTERONE IN PROSTAGLANDIN-TREATED BEEF HEIFERS

2005 ◽  
Vol 17 (2) ◽  
pp. 160
Author(s):  
M. Martínez ◽  
M. Caccia ◽  
M. Colazo ◽  
G. Bó ◽  
J. Kastelic ◽  
...  
Keyword(s):  
Animal Health ◽  
Follicular Development ◽  
Estradiol Benzoate ◽  
Beef Heifers ◽  
Once Daily ◽  
Treatment Groups ◽  
Dose Time ◽  
Follicular Wave ◽  
Time Interaction ◽  
To Receive

Estradiol and progesterone have been used to synchronize follicular wave emergence and ovulation in a two-dose prostaglandin (PGF)-based synchronization program (Martínez et al. 2004 Theriogenology 62, 363–372). However, it was observed that some heifers displayed estrus prior to the second PGF, suggesting that premature luteolysis may have occurred. An experiment was designed to determine the effects of dose of estradiol benzoate (EB) and/or progesterone (P) on follicular and luteal dynamics in a two dose PGF-based protocol in beef heifers. In two replicates, beef heifers (n = 28; Simmental, Hereford, and Charolais crosses, 350 to 450 kg) received 500 μg cloprostenol (Schering-Plough Animal Health, Pointe-Claire, PQ, Canada) on Day −7. On Day 0, heifers were randomly allocated to nine treatment groups to receive 0, 1, or 2 mg of EB and 0, 50, or 100 mg of P i.m. in canola oil in a 3 × 3 factorial design. A second PGF treatment was administered on Day 14. Ultrasonography was done once daily from Days −5 to 9, and every 12 h thereafter until ovulation. Blood samples were collected at 12-hour intervals from Day 0 to 5 for estradiol and FSH concentrations, and every 24 h for progesterone. The effects of EB and P and their interaction on corpus luteum (CL), follicles, and hormone profiles were analyzed by analysis of variance, and means were compared by LSD or Tukey's test. All variables were normally distributed (Wilk-Shapiro test and rankit plots). The day of follicular wave at the time of treatment tended to vary among groups (P = 0.08) and the diameter of the dominant follicle also differed (P < 0.05). The interval from treatment to wave emergence was shorter (P < 0.05) in heifers that received 2 mg EB (4.6 ± 0.3 d) than in those that did not receive EB (5.9 ± 0.6 d), while the 1 mg EB group (5.1 ± 0.6 d) was intermediate. The interval to wave emergence in the 2 mg EB group was the least variable (P < 0.05). There was no effect of EB (P = 0.72) on the diameter of the CL at the time of the second PGF, but there was an effect of P treatment (P = 0.01). The variability of the interval from the second PGF to ovulation may have been influenced by treatment group but was statistically not significant (P < 0.1). There was an effect of time (P < 0.01) on plasma progesterone concentrations and a P × time interaction (P = 0.06) can also be assumed. Estradiol concentrations were affected by EB dose, time, and EB × time interaction (all P < 0.01). FSH concentrations were modified by time (P < 0.01). In summary, treatment with EB 7 days after a single injection of PGF affected follicular development, while P treatment at that time appeared to influence CL function.

9 COMPARATIVE EFFICACY OF AN 8-DAY Cue-Mate®/ESTRADIOL BENZOATE PROGRAM WITH OR WITHOUT INCLUSION OF EQUINE CHORIONIC GONADOTROPIN IN ANESTROUS DAIRY COWS

2008 ◽  
Vol 20 (1) ◽  
pp. 85 ◽  
Author(s):  
M. A. Bryan ◽  
R. Emslie ◽  
C. Heuer
Keyword(s):  
Confidence Interval ◽  
Dairy Cows ◽  
Animal Health ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Adjusted Relative Risk ◽  
Reproductive Response ◽  
Mate Removal ◽  
Equine Chorionic Gonadotropin ◽  
Rectal Palpation

The objective was to quantify and compare the reproductive response of lactating, seasonally calving dairy cows diagnosed with anovulatory anestrus (AA) to treatment with two separate progesterone (Cue-Mate�, Bioniche Animal Health, Australasia, Armidale, NSW, Australia) and oestradiol benzoate (ODB) treatment regimes, either including or excluding eCG. Cows on six commercial seasonal-calving dairy farms in New Zealand (n = 2355) were presented following 3 weeks of tailpainting and a subsequent non-observed estrus. Cows which were confirmed in anestrus with no detectable corpus luteum (CL) by rectal palpation were selected for trial inclusion (n = 1382). All cows received the standard anestrous protocol, consisting of an 8-day insertion of the progesterone device containing 1.56 g of progesterone (Cue-Mate; injection of 2 mg of ODB at the time of insertion and further injection of 1 mg of ODB 24 h after Cue-Mate removal (Day 9)). Approximately half of the cows were randomly allocated (using the RAND function within Excel, Microsoft) to a further intervention consisting of an injection of 400 IU of eCG (Pregnecol�, Bioniche) on the day of Cue-Mate removal (Day 8). This became the Treatment group with the standard treatment the Control group. Cows were then mated to detected heats; all cows not detected in estrus and inseminated were blanket-mated 36 h after the second ODB treatment. Cows were scanned ultrasonically 10 weeks post-treatment and all pregnancies were aged to the nearest half-week. Where two matings were recorded within 7 days of each other, the later mating was used. Data were analyzed using both a matched logistic regression analysis and survival analysis, using SPSS (SPSS.com) and SAS (SAS.com). Pregnecol-treated cows were more likely to conceive in the first 48 h following treatment (48.84% v. 43.10%; P = 0.06), and were more likely to be in calf within 7 days following treatment (P = 0.07). The effect of Pregnecol was greater in cows over 5 years of age, such that this stratum of cows was significantly more likely to conceive within 48 h [58.33% v. 38.10%; P = 0.00; adjusted relative risk (RR) 1.51; 95% lower confidence interval (LCI) 1.15; 95% upper confidence interval (UCI) 2.00], and was significantly more likely to be in calf within 7 days (55.20% v. 37.86%; P = 0.00; RR 1.44; LCI 1.41; UCI 1.82). Pregnecol-treated cows over 5 years of age were significantly more likely to be in calf at 4 weeks (72.22% v. 59.57%; P = 0.02; RR 1.21; LCI 1.03; UCI 1.42). Cows that had calved less than 50 days were significantly less likely to be in calf by 4 weeks (62.26% v. 69.35%; P = 0.04). In summary, the addition of a 400 IU treatment of eCG (Pregnecol) at the time of Cue-Mate removal in a standard 8-day progesterone device/ODB anestrous treatment program increased the risk of pregnancy. The effects of eCG on cows over 5 years of age were very strong and highly significant.

scholarly journals Do EFL Learners Enhance Their knowledge of Their Home Cultures as a Result of comparing them with other Cultures Through Project-Based Learning?

2021 ◽  
Vol 04 (08) ◽  
Author(s):  
Akharraz Mohamad ◽  
Keyword(s):  
Language Learners ◽  
Cultural Awareness ◽  
Cultural Practices ◽  
Project Based Learning ◽  
Control Group ◽  
Cultural Products ◽  
Treatment Groups ◽  
Foreign Language Learners ◽  
The Impact ◽  
Experimental Group

The existing research studies have revealed that project-based learning (PjBL) has significantly helped foreign language learners enhance their cultural understanding as a result of completing their projects (e.g., Bouchouk, 2017; Hsu, 2014; Kim, 2019; Liu et al., 2006; Ngo, 2014). While these studies have congruently proved the utility of PjBL in enhancing students’ cultural awareness, none of them has measured the effect of PjBL together with a comparative and contrastive approach on students’ understanding of their home cultures. Hence, the purpose of this study is to investigate the impact of comparing students’ home cultures with the English cultures through PjBL on students’ understanding of their own cultures in terms of cultural products, cultural practices, and cultural perspectives. Two similar intact groups were randomly assigned to control and treatment groups. The two groups were pretested, administered two different treatments for 24 weeks, and post-tested to assess their differences. The independent sample t-tests results exhibited that the experimental group substantially deepened their understanding of their home cultures as a result of comparing them with the English cultures through PjBL. The study also revealed that while the control group significantly improved their cultural products, those in the experimental group greatly enhanced their knowledge of cultural practices and perspectives.

scholarly journals Effects of Enriched Charcoal as Permanent 0.2% Feed-Additive in Standard and Low-Protein Diets of Male Fattening Turkeys: An On-Farm Study

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 541
Author(s):  
Hinz ◽  
Stracke ◽  
Schättler ◽  
Kemper ◽  
Spindler
Keyword(s):  
Animal Health ◽  
Matter Content ◽  
Feed Additive ◽  
Standard Diet ◽  
Dry Matter Content ◽  
Positive Effects ◽  
Low Protein ◽  
Protein Reduction ◽  
On Farm ◽  
Experimental Group

Wet litter is the most important cause of footpad dermatitis in poultry, this in turn being a highly relevant animal-related welfare indicator. This field study was subdivided into two experiments. In Experiment 1, the standard diet was supplemented by 0.2% enriched charcoal, being a non-specific absorber and therefore might be promising in reducing faecal moisture. In Experiment 2, the experimental group received a reduced crude protein diet during weeks 6–13, combined with a 0.2% enriched charcoal supplementation. The trials were each conducted with two batches on three farms under on-farm conditions. The animals were observed at 6, 10, 14 and 18 weeks of age to collect data on body weight and different health parameters. The mortality and litter samples were analysed after slaughtering. In Experiment 1, performance and health were not affected despite higher dry matter content of the litter. In Experiment 2, the weight of birds receiving the protein-reduced diet was decreased significantly throughout the experiment. However, the slaughter weight did not differ. The mortality was reduced by 0.5% in the experimental group. Therefore, it was concluded that 0.2% of enriched charcoal is not a valuable feed-additive regarding animal health, while temporary protein reduction might have positive effects.

scholarly journals The Influence of the Mineral-Microbial Preparation on Ammonia Concentration and Productivity in Laying Hens Houses

Atmosphere ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 751 ◽  
Author(s):  
Remigiusz Gałęcki ◽  
Michał Dąbrowski ◽  
Tadeusz Bakuła ◽  
Kazimierz Obremski ◽  
Adriana Nowak ◽  
...  
Keyword(s):  
Body Weight ◽  
Linear Correlation ◽  
Egg Production ◽  
Laying Hens ◽  
Animal Health ◽  
The Body ◽  
Control Group ◽  
Microbial Composition ◽  
Experimental Group ◽  
Campylobacter Spp

The aim of this study was to evaluate the influence of the biopreparation Deodoric® on ammonia (NH3) concentration, performance, and hygiene standards in laying hen (ROSS-308) production. Statistically significant differences in NH3 concentration and the body weight of laying hens were observed between the control group (C) and the experimental group (E) where Deodoric® was applied at the set dose. In the control group, an increase in NH3 concentration could have contributed to the decrease in the body weight of laying hens, egg production, and % hen day egg production, whereas no such correlations were observed in the experimental group. A moderate linear correlation between NH3 concentration vs. humidity (r = 0.68), air flow (r = 0.48) and weakly linear correlation between NH3 concentration and age of birds (r = 0.27) was noted in group C. In group E, NH3 concentration vs. temperature (r = 0.27) and humidity (r = 0.14) were weakly correlated. Statistical analysis of changes in the microbial counts isolated from manure revealed a significant decrease of mesophilic microorganisms on day 28 decrease of Campylobacter spp. days 14 and 84 in group E. However, for the entire experimental model no statistically significant changes in the number of Campylobacter spp. and mesophilic bacteria were found. The tested preparation did not cause changes in the microbial composition of tissue swabs. Deodoric® contributes to animal welfare by reducing the ammonia concentrations in poultry houses. It is also recommended for use in poultry farms to improve animal health and performance and to generate benefits for producers.

scholarly journals Anthelminthic resistance of gastrointestinal nematodes in sheep to monepantel treatment in central region of Rio Grande do Sul, Brazil

2018 ◽  
Vol 38 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Fernanda Ramos ◽  
Luiza P. Portella ◽  
Fernando de S. Rodrigues ◽  
Caroline Z. Reginato ◽  
Alfredo S. Cezar ◽  
...  
Keyword(s):  
Rio Grande ◽  
Gastrointestinal Nematodes ◽  
Infected Sheep ◽  
The Other ◽  
Control Group ◽  
Fecal Culture ◽  
Rio Grande Do Sul ◽  
Anthelminthic Resistance ◽  
On Farm ◽  
Experimental Group

ABSTRACT: Given the numerous reports of anthelminthic resistance of sheep nematodes to different anthelmintic compounds, this study aimed to evaluate the resistance status of gastrointestinal nematodes from naturally infected sheep to monepantel in the state of Rio Grande do Sul. Four farms that present extensive raising system and absence of anthelmintic treatment for 60 days were selected for the study. Lambs that present counts of eggs per gram of feces (EPG) ≥200 (sensitivity of 50 EPG) one day (D-1) before the treatment were select for the study and randomly separated into two groups, a control group and an experimental group treated with monepantel. Feces were collected 9 days after the treatment (D+9) for EPG counts and fecal culture. The monepantel was 100% effective only on 2. The efficacy found on farm 1, 3, and 4 were 2.82%, 25.8%, and 78.4%, respectably. There were no viable larvae post-treatment at farm 2, but the genera Haemonchus, Trichostrongylus, Cooperia, and Strongyloides were resistant to it at the other farms. This study shows the presence of parasites resistant to the treatment with monepantel, pointing to the importance of monitoring its efficacy in sheep flocks of Rio Grande do Sul, Brazil.

287 EFFECT OF FOLLICULAR WAVE SYNCHRONIZATION AND ABSENCE OF CORPUS LUTEUM ON COC RECOVERY IN Gyr (BOS TAURUS INDICUS) COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 300
Author(s):  
T. Miyauchi ◽  
C. A. C. Fernandes ◽  
E. R. Oliveira ◽  
B. F. L. Alves ◽  
J. H. M. Viana
Keyword(s):  
Bos Taurus ◽  
Animal Health ◽  
Cumulus Cell ◽  
Estradiol Benzoate ◽  
Corpora Lutea ◽  
Portable Ultrasound ◽  
Follicular Wave ◽  
Main Technique ◽  
Oocyte Donors

Transvaginal guided follicle aspiration (TGFA) is the main technique used in Brazil to recover oocytes for in vitro embryo production (IVP) in bovine. Different protocols have been proposed to synchronize follicular emergence in oocyte donors, but most of them were developed for use in European breeds of cattle, which show many differences in ovarian physiology when compared with Zebu breeds. The aim of this study was to compare different protocols for preparation of Gyr (dairy zebu breed) oocyte donors. The TGFA were performed in a donor management facility located in Minas Gerais State, southeast Brazil. Pluriparous cycling Gir cows (n = 42) were used as donors. All cows underwent 3 treatments: G1 (control), no treatment before TGFA; G2, 2 mg of estradiol benzoate (EB) for follicular wave synchronization given i.m. 5 days before TGFA; and G3, norgestomet auricular implants given 9 days and 2 mg of EB plus 0.53 mg of cloprostenol given 5 days before TGFA. The interval between TGFA in the same donor was greater than 30 days. All procedures were made by the same technician, using a portable ultrasound device, disposable 19G or 20G needles, and a vacuum pressure of 80 mm Hg. The aspirated follicular fluid was collected in 50-mL Falcon tubes and sent to the laboratory for COC identification and classification under 50 × magnification. Recovered oocytes were classified according to cumulus cell layers and cytoplasm morphology. The total number of oocytes and viable COC recovered and the procedure length (min), including time spent for TGFA and laboratory manipulation, were compared. Data were evaluated by ANOVA, and means compared by Tukey’s test. A total of 126 TGFA sessions were performed, with recovery of 2,809 oocytes (20.31 ± 12.32 of COC and 14.83 ± 7.97 of viable COC per cow/session; mean ± SD). The total number of recovered oocytes and viable COC recovered were lower in G1 compared with G2 and G3 (15.18 ± 11.07 v. 21.18 ± 9.71 and 24.68 ± 9.03; and 9.53 ± 7.22 v. 16.97 ± 6.47 and 18.84 ± 8.90, respectively; P < 0.05) There was no difference (P > 0.05) between G2 and G3 on the number of oocytes or viable COCs recovered. The procedure length, however, was longer in G1 and G2 compared with G3 (49.6 ± 15.1 and 46.9 ± 13.4 v. 35.8 ± 13.1 min, respectively; P < 0.05) The shorter procedure length in G3 was probably associated with the reduced number of cows showing no corpora lutea (38/42, 90%), which resulted in reduction of bleeding and clot formation in the aspirated fluid. These results show that (1) previous follicular wave synchronization by EB can improve the number and quality of recovered oocytes; (2) the absence of corpora lutea does not increase the number of recovered COC and viable COC, but reduces the time spent in the procedure. Intervet Schering Plough Animal Health.

16 STRATEGIES OF FOLLICULAR WAVE SYNCHRONIZATION WITH ESTRADIOL BENZOATE IN GYR (BOS TAURUS INDICUS) CATTLE

2013 ◽  
Vol 25 (1) ◽  
pp. 155
Author(s):  
L. D. P. Sinedino ◽  
B. T. Gerhardt ◽  
J. A. Moura ◽  
A. P. Dourado ◽  
I. L. Goulart ◽  
...  
Keyword(s):  
Blood Sample ◽  
Estrous Cycle ◽  
Bos Taurus ◽  
Animal Health ◽  
Estradiol Benzoate ◽  
Wilcoxon Test ◽  
Second Step ◽  
Follicular Atresia ◽  
Follicular Wave ◽  
Animal Category

Physiological and behavioral differences between Bos taurus and Bos indicus can influence the response to a fixed AI protocol. The objective of this study was to examine the reduction of the usual dose of 2 mg of estradiol benzoate (EB) to 1 mg at the beginning of a fixed-time AI protocol, aiming at follicular regression. In a second step, we evaluated the effect of EB on follicle development during follicular pre-deviation and dominance. The experiment was performed at Monte Verde Farm (Uberaba, MG, Brazil). Twenty-two cows (n = 10 heifers and n = 12 nonlactating cows) underwent an estrous synchronization protocol with a progesterone-releasing intravaginal device (P4; Sincrogest®, Ouro Fino Animal Health, São Paulo, Brazil) and received 1 (G1mg, n = 11) or 2 mg (G2mg, n = 11) of EB (Sincrodiol®, Ouro Fino Animal Health), on a random day (designated Day 0). Follicular dynamics was monitored once per day by ultrasonography from Day 0 to 4 with blood sample collections. In a second step, females received 2 mg of EB on Day 3 (GD3, pre-deviation, n = 4) or Day 5 (GD5, dominance, n = 4) of the estrous cycle (Day 0 was the ovulation). Following these treatments, follicular development was monitored daily for 6 days with blood sample collections. The statistical analysis was conducted using the SAS System for Windows 2 (2003; SAS Institute Inc., Cary, NC, USA). The explanatory variables included in the statistical model were the dose of EB, animal category (cows and heifers), and their interaction. The mean test was used to compare intervals from EB treatment to follicular atresia and follicular wave emergence using ANOVA. Progesterone concentrations between groups were compared using the Wilcoxon test. Independently of animal category or stage of the estrous cycle, both EB doses (1 or 2 mg) induced follicular atresia in 2.2 ± 0.9 and 2.1 ± 1.2 days (P > 0.05), respectively. Emergence of a new follicular wave was observed, from Day 0 to 4, in 64% (7/11) of females from G1mg and in 45% (5/11) from G2mg, and the interval between treatment and follicular emergence was 3.4 ± 0.8 and 3.0 ± 1.0 days (P > 0.05), respectively. Plasma progesterone concentrations of the 22 animals increased from 2.1 ± 2.0 ng mL–1 to 7.6 ± 3.0 ng mL–1 by 24 h after the device insertion (P < 0.05), reaching peak concentration (8.0 ± 3.0 ng mL–1) by 48 h after treatment beginning, decreasing to 6.4 ± 2.5 ng mL–1 by 72 h, and remaining constant up to 96 h. Estradiol benzoate injection at follicle pre-deviation (GD3) caused follicular atresia (2.0 ± 1.4 days) and emergence of a new follicular wave in 3.7 ± 0.1 days in all animals (4/4). However, EB injection during follicle dominance (GD5) did not synchronize a new follicular wave and follicles persisted during the time of monitoring. Furthermore, EB applied at dominance hastened luteolysis in 50% (2/4) of the treated animals. In conclusion, a reduced dose of EB (1 mg) at the beginning of the protocol with P4 effectively induces follicular atresia. To synchronize a wave emergence at any stage of the estrous cycle, EB must be associated with an exogenous source of progesterone.

406 EFFECT OF DIFFERENT FOLLICLE-STIMULATING HORMONE DOSES ON SUPEROVULATORY RESPONSE OF Red Sindhi COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 359
Author(s):  
B. P. Carvalho ◽  
M. R. B. Mello ◽  
J. M. Baldrighi ◽  
J. S. Campanati ◽  
R. R. C. Mello ◽  
...  
Keyword(s):  
Animal Health ◽  
Follicle Stimulating Hormone ◽  
Reproductive Potential ◽  
Estradiol Benzoate ◽  
Bovine Embryo ◽  
Treatment Groups ◽  
Adequate Dosage ◽  
Level Of Significance ◽  
Cryopreserved Embryos ◽  
Stimulating Hormone

The use of bovine embryo transfer to increase the reproductive potential of animals with high genetic merit is unquestionable. However, studies that evaluate the response to exogenous FSH stimulation in Red Sindhi cows are rare. Therefore, the objective of this study was to compare the superovulatory response of Red Sindhi cows treated with 3 different doses of FSH (Folltropin®-V, Bioniche Animal Health, Belleville, Ontario, Canada). The animals used had body condition scores ranging from 3.5 to 4.0 (1-5 scale), were between 6 and 10 years of age, and were randomly assigned to 1 of 3 treatment groups. Three doses of FSH were administered: 100 mg (n = 16); 133 mg (n = 13), and 200 mg (n = 14). The treatments were initiated on random days of the estrous cycle. On Day 0, animals received 2 mg of estradiol benzoate i.m. (Estrogin®, Farmavet, Jaboticabal, Brazil) and an intravaginal device (CIDR; InterAg, Hamilton, New Zealand) with 1.9 g of progesterone. On Day 4, donors received FSH in 8 i.m. injections that decreased in dose during 4 consecutive days. A dose of 265 μg of cloprostenol (Ciosin®, Schering-Plough, Cotia, Brazil) was given i.m. concomitantly with the fifth FSH injection. Twelve hours after the last FSH injection, donors received 25 μg of lecirelin i.m. (GnRH; Gestran Plus®, ARSA SRL, Buenos Aires, Argentina). TheAI were performed 12 and 24 h after GnRH. Seven days after the first AI, embryos were recovered and classified as described in the IETS manual. Data were evaluated using KruskalWallis test at a 5% level of significance. Ovarian ultrasonography was also performed to count the number of CL present at the time of ova/embryo collection. Results are summarized in Table 1. The number of CL and total ova/embryos was higher in donors receiving 200 mg of FSH than in those in the other groups. However, the number of transferable or cryopreserved embryos did not differ among groups. In conclusion, the dosage of 200 mg of Folltropin®-V seemed to be the more adequate dosage for this breed of cattle. Table 1.Effect of different follicle-stimulating hormone (FSH) doses on CL number, total structures, transferable embryos, and cryopreserved embryos in Red Sindhi cows (mean ± SEM) Financial support provided by FAPERJ (E26-170.529/2007).

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