159 TECHNIQUES FOR OVUM PICK-UP IN GONADOTROPIN-TREATED ALPACAS

2008 ◽  
Vol 20 (1) ◽  
pp. 159
Author(s):  
G. Gamarra ◽  
A. Gallegos ◽  
E. Alvarado ◽  
M. Asparrin ◽  
W. Vivanco
Keyword(s):  
Transvaginal Ultrasound ◽  
Animal Health ◽  
Cumulus Cell ◽  
Estradiol Benzoate ◽  
Ultrasound Guided ◽  
Ringer’S Lactate ◽  
Cell Layers ◽  
The Mean ◽  
Intravaginal Device

The objective of the present study was to evaluate the quantity and quality of oocytes collected when using 2 methods for ovum pick-up and 2 different regimens for ovarian stimulation in live alpaca donors. Thirty-four non-pregnant female alpacas of 3 to 5 years of age maintained at 4100 m elevation in southern Peru were randomly distributed into 4 experimental groups. Groups 1 (n = 8) and 3 (n = 9) received an intravaginal device containing 0.78 mg of progesterone (Cue-Mate�, Bioniche Animal Health, Belleville, Ontario, Canada) plus an i.m. injection of 1 mg of estradiol benzoate on Day 0; the intravaginal device was removed on Day 7. Groups 2 (n = 7) and 4 (n = 10) received an i.m. injection of 3.1 mg of LH (Lutropin�, Bioniche Animal Health) on Day 0. Females received 700 IU of eCG (Pregnecol�, Bioniche Animal Health) i.m. on Day 7 (Groups 1 and 3) or Day 2 (Groups 2 and 4). In all groups, oocyte collection was done 2 days after the injection of eCG. Groups 1 and 2 were subjected to ventral laparotomy aspirating the oocytes from follicles >3 mm in diameter using a 10-mL hypodermic syringe containing 1 mL of aspiration media (Ringer's lactate solution plus 10% bovine serum) and connected to an 18 G � 1 inch aspiration needle. After collection, the follicular fluid was searched and the COC were graded. Groups 3 and 4 were subjected to ovum pick-up by transvaginal recovery using an ultrasound scanner (Parus 240�, Pie Medical, Maastricht, the Netherlands) equipped with a vaginal probe of 7.5 MHz (MEVA�, Pie Medical) and a 17G � 55 cm aspiration needle introduced through a needle guide. Follicles >3 mm in diameter were aspirated into 50-mL centrifuge tubes containing 5 mL of aspiration media with 75 IU mL–1 of heparin. The aspirated fluid was filtered and rinsed using an embryo filter (EmCon�, Immunosystems, Menomonie, WI), and COC were searched and graded under a microscope based on the intactness of the cumulus cell layers. Data were analyzed by ANOVA. There were no differences (P > 0.05) between groups in the mean number of follicles aspirated per donor (11.0, 13.8, 9.4, and 9.1 for Groups 1 to 4 respectively), and in the mean number of COC recovered per donor (7.6, 7.0, 6.0, and 6.1 respectively for Groups 1 to 4). The proportions of good quality COC were significantly (P < 0.01) different between surgical (81.0 and 79.5% for Groups 1 and 2) and transvaginal/ultrasound-guided (7.4% for Group 3) methods of collection; however, they were similar to the proportion in Group 4 (64.9%) retrievals. The results show that in the absence of an intravaginal device, a similar quantity and quality of alpaca oocytes can be collected when using a surgical approach or minimally invasive ultrasound-guided transvaginal follicular aspiration.

200 EVALUATION OF SUPEROVULATORY REGIMES FOR IN VIVO EMBRYO PRODUCTION IN ALPACAS (LAMA PACOS)

2010 ◽  
Vol 22 (1) ◽  
pp. 258
Author(s):  
H. W. Vivanco ◽  
E. Huaman ◽  
S. Leon ◽  
A. Gallegos ◽  
M. Asparrin ◽  
...  
Keyword(s):  
Animal Health ◽  
Estradiol Benzoate ◽  
Preovulatory Follicle ◽  
Embryo Production ◽  
Removal Time ◽  
The Mean ◽  
The One ◽  
Intravaginal Device ◽  
Lama Pacos

The objective of the study was to evaluate 4 superovulatory regimes in terms of the quantity of transferable embryos recovered. A total of 48 female alpacas, 3 to 5 years of age and located at Malkini Alpacas Farm (4100 m elevation), were distributed into 4 treatments. In treatment 1, 13 female alpacas received on Day 0 an intravaginal device containing 0.78 mg of progesterone (Cue Mate®, Bioniche Animal Health, Belleville, Ontario, Canada) followed immediately by an i.m injection of estradiol (1 mg of estradiol benzoate) and an i.m. injection of PGF2α (Veyx®, 0.25 mg of cloprostenol). The intravaginal device was removed on Day 7, performing at removal time an i.m. injection of estradiol. From Days 8 to 16, the alpacas received an i.m injection twice per day and 12 hours apart of pFSH (FolltropinV®, Bioniche Animal Health) in decreasing doses totaling 420 mg of pFSH; on Day 16,300 IU of eCGi.m. (Pregnecol®, Bioniche Animal Health) was injected. In treatment 2, 13 alpacas received on Day 0 an intravaginal device of progesterone followed by an i.m. injection of PGF2; from Days 5 to 9, alpacas received injections twice per day of decreasing doses of pFSH (porcine FHS) totaling 320 mg; on Day 7, the intravaginal device was removed and 500 IU i.m. of eCG was injected. In treatment 3,13 alpacas received on Day 0 an intravaginal device of progesterone followed immediately by an i.m injection of GnRH (Conceptal®, 0.0042 mg of acetate of busereline); pFSH was injected i.m. from Days 5 to 9 in decreasing doses twice per day, totaling 440 mg; the intravaginal device was removed on Day 7. In treatment 4, 9 female alpacas received on Day 0 an i.m. injection of GnRH after verifying the presence of a preovulatory follicle (>8.0 mm diameter). On Day 2, the alpacas received 1000 IU i.m. of eCG followed on Day 7 by an i.m. injection of PGF2. In all cases, the donor alpacas were evaluated by ultrasonography. The matings for treatments 1, 2, and 3 were performed twice per donor alpaca at 12-hour intervals between Days 5 and 8 of the initiation of the pFSH treatments, whereas in treatment 4 the matings were made the following day after the application of the PGF2. In treatment 1, the donor alpacas received at time of first mating an i.m injection of 3.75 mg of LH (Lutropin®, Bioniche Animal Health); in treatments 2, 3, and 4, the donors received an i.m. injection of GnRH. In all treatments, embryo collection was performed by nonsurgical method 6.5 days after first mating. There were significant differences between treatments (P < 0.05) in the mean number of CL, with treatment 4 being the highest (4.7 ± 2.63, 4.1 ± 3.05, 1.8 ± 1.8, and 6.0 ± 3.16 for treatments 1 to 4, respectively). The total number of blastocysts recovered per treatment was 7, 16, 2, and 18 for treatments 1 to 4, respectively. The superovulatory strategy followed for treatment 4 showed to be the one resulting in the highest number of transferable embryos. Further comparative evaluations between FSH and eCG treatments are recommended. Research was partially funded by the contributions of Bioniche Animal Health.

122 TIMED ARTIFICIAL INSEMINATION IN WOOD BISON USING FROZEN-THAWED SEMEN

2016 ◽  
Vol 28 (2) ◽  
pp. 191 ◽  
Author(s):  
G. P. Adams ◽  
S. X. Yang ◽  
J. M. Palomino ◽  
M. Anzar
Keyword(s):  
Animal Health ◽  
Egg Yolk ◽  
Ovulation Rate ◽  
Chi Square ◽  
Semen Cryopreservation ◽  
Wood Bison ◽  
The Mean ◽  
Intravaginal Device ◽  
Synchronization Procedure

Recent progress with methods to control ovulation and semen cryopreservation in Wood Bison was the impetus to test the feasibility of timed AI to facilitate reclamation of this threatened species. A 2 × 2 design was used to compare the efficacy of 2 ovulation synchronization techniques and 2 semen cryopreservation protocols. Female Wood Bison were assigned randomly to 2 groups (n = 24/group) in which ovarian synchronization was induced by ultrasound-guided ablation of follicles >5 mm or intramuscular treatment with 2.5 mg of estradiol 17B + 50 mg of progesterone (E+P) in canola oil. A progesterone-releasing intravaginal device (PRID) was placed at the time of follicle ablation (for 5 days) or E+P treatment (for 8 days) in the respective groups. A luteolytic dose of prostaglandin was given at the time of PRID removal, and 2500 IU of hCG was given IM 3 days later. Bison were inseminated 24 and 36 h after hCG treatment using frozen-thawed semen. The semen was collected by electro-ejaculation from 4 Wood Bison bulls, pooled, and divided into aliquots diluted in either egg-yolk extender (EY) or cholesterol-loaded cyclodextrin extender (CLC). Half the bison in each synchronization group were inseminated with either EY- or CLC-extended semen. Bison were examined by ultrasonography every 12 h beginning on the day of hCG treatment for 3 days or until ovulation was detected, whichever occurred first. Pregnancy diagnosis was made by ultrasonography 34–36 days after insemination. Two bison were excluded during the experiment because of handling difficulty; therefore, the total number of bison used was 46. Ovulation rate and interval to ovulation were compared between synchronization groups by chi-square and t-test, respectively. Pregnancy rates were compared among groups by 2-way ANOVA after transforming data to arcsin. The ovulation rate was not different between synchronization groups [combined mean, 37/46 (80%)], nor was the degree of synchrony, as assessed by the residuals (variation from the mean) in the respective groups. However, the diameter (mean ± standard error of the mean) of the dominant follicle at the time of hCG treatment was smaller in the follicle ablation group than in the E+P group (10.5 ± 0.6 v. 13.9 ± 0.6; P < 0.04), and the interval from hCG treatment to ovulation tended to be longer (35.3 ± 1.6 v. 31.8 ± 1.3 h; P ≤ 0.10). Pregnancy rate was not affected by synchronization procedure, but pregnancy was detected only in the EY-inseminated group (9/23 v. 0/23; P < 0.01). Despite that post-thaw sperm motility was similar for EY and CLC semen (41.7 ± 2.9 and 44.6 ± 3.3%; respectively), CLC-treated semen failed to impregnate bison in vivo. We concluded that synchronization and timed insemination with frozen-thawed semen is feasible in Wood Bison. Of the 23 bison inseminated with EY-extended semen, 21 ovulated (91%), and of those that ovulated 9 became pregnant (43%). Both synchronization schemes were effective, but the ablation protocol may be improved by an additional day between ablation and hCG treatment. We thank Vetoquinol Canada and Merck Animal Health for providing hormone treatments.

287 EFFECT OF FOLLICULAR WAVE SYNCHRONIZATION AND ABSENCE OF CORPUS LUTEUM ON COC RECOVERY IN Gyr (BOS TAURUS INDICUS) COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 300
Author(s):  
T. Miyauchi ◽  
C. A. C. Fernandes ◽  
E. R. Oliveira ◽  
B. F. L. Alves ◽  
J. H. M. Viana
Keyword(s):  
Bos Taurus ◽  
Animal Health ◽  
Cumulus Cell ◽  
Estradiol Benzoate ◽  
Corpora Lutea ◽  
Portable Ultrasound ◽  
Follicular Wave ◽  
Main Technique ◽  
Oocyte Donors

Transvaginal guided follicle aspiration (TGFA) is the main technique used in Brazil to recover oocytes for in vitro embryo production (IVP) in bovine. Different protocols have been proposed to synchronize follicular emergence in oocyte donors, but most of them were developed for use in European breeds of cattle, which show many differences in ovarian physiology when compared with Zebu breeds. The aim of this study was to compare different protocols for preparation of Gyr (dairy zebu breed) oocyte donors. The TGFA were performed in a donor management facility located in Minas Gerais State, southeast Brazil. Pluriparous cycling Gir cows (n = 42) were used as donors. All cows underwent 3 treatments: G1 (control), no treatment before TGFA; G2, 2 mg of estradiol benzoate (EB) for follicular wave synchronization given i.m. 5 days before TGFA; and G3, norgestomet auricular implants given 9 days and 2 mg of EB plus 0.53 mg of cloprostenol given 5 days before TGFA. The interval between TGFA in the same donor was greater than 30 days. All procedures were made by the same technician, using a portable ultrasound device, disposable 19G or 20G needles, and a vacuum pressure of 80 mm Hg. The aspirated follicular fluid was collected in 50-mL Falcon tubes and sent to the laboratory for COC identification and classification under 50 × magnification. Recovered oocytes were classified according to cumulus cell layers and cytoplasm morphology. The total number of oocytes and viable COC recovered and the procedure length (min), including time spent for TGFA and laboratory manipulation, were compared. Data were evaluated by ANOVA, and means compared by Tukey’s test. A total of 126 TGFA sessions were performed, with recovery of 2,809 oocytes (20.31 ± 12.32 of COC and 14.83 ± 7.97 of viable COC per cow/session; mean ± SD). The total number of recovered oocytes and viable COC recovered were lower in G1 compared with G2 and G3 (15.18 ± 11.07 v. 21.18 ± 9.71 and 24.68 ± 9.03; and 9.53 ± 7.22 v. 16.97 ± 6.47 and 18.84 ± 8.90, respectively; P < 0.05) There was no difference (P > 0.05) between G2 and G3 on the number of oocytes or viable COCs recovered. The procedure length, however, was longer in G1 and G2 compared with G3 (49.6 ± 15.1 and 46.9 ± 13.4 v. 35.8 ± 13.1 min, respectively; P < 0.05) The shorter procedure length in G3 was probably associated with the reduced number of cows showing no corpora lutea (38/42, 90%), which resulted in reduction of bleeding and clot formation in the aspirated fluid. These results show that (1) previous follicular wave synchronization by EB can improve the number and quality of recovered oocytes; (2) the absence of corpora lutea does not increase the number of recovered COC and viable COC, but reduces the time spent in the procedure. Intervet Schering Plough Animal Health.

Cloned horse pregnancies produced using adult cumulus cells

2004 ◽  
Vol 16 (7) ◽  
pp. 675 ◽  
Author(s):  
Dirk K. Vanderwall ◽  
Gordon L. Woods ◽  
Kenneth I. Aston ◽  
Thomas D. Bunch ◽  
Guanpeng Li ◽  
...  
Keyword(s):  
Survival Rate ◽  
Nuclear Transfer ◽  
Transvaginal Ultrasound ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Ultrasound Guided ◽  
Embryo Survival ◽  
Donor Cells ◽  
Follicle Aspiration

The objectives of the present study were to: (1) clone horses using adult cumulus cells; and (2) determine whether the cumulus cell donor affected the outcome. In vivo-matured cumulus–oocyte complexes were obtained using transvaginal ultrasound-guided follicle aspiration; oocytes were used as cytoplasts, whereas cumulus cells (from one of three different mares) were used as donor cells. Immediately following nuclear transfer and activation procedures, cloned embryos were transferred surgically to the oviduct of recipient mares (n = 2–5 embryos per recipient) that had ovulated within 24 h prior to the transfer. An initial pregnancy examination was performed between Days 14 and 16 (Day 0 = surgery); subsequent examinations were then performed every 7–10 days. A total of 136 follicles were aspirated in 96 mares, from which 72 oocytes were recovered (53%). Sixty-two cloned embryos were transferred to recipient mares, which resulted in seven (11.3%) ultrasonographically detectable conceptuses between Days 14 and 16. All seven conceptuses were lost spontaneously between Days 16 and 80. Cumulus cells from Mare 160 tended (P = 0.08) to result in a higher embryo survival rate than cumulus cells from Mare 221 (4/17 v. 1/25 respectively). To our knowledge, this is the first report documenting the establishment of cloned equine pregnancies derived from adult cumulus cells.

299 OVUM PICK-UP USE IN NELORE COWS SUPERSTIMULATED WITH FSH OR eCG

2010 ◽  
Vol 22 (1) ◽  
pp. 306 ◽  
Author(s):  
L. V. P. Ribeiro ◽  
L. P. Rigolon ◽  
F. L. B. Cavaliere ◽  
A. C. Martinez ◽  
R. R. Martins ◽  
...  
Keyword(s):  
Bos Indicus ◽  
Reproductive Technologies ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Average Weight ◽  
The Mean ◽  
Group 2 ◽  
Reproductive Rates ◽  
Mean Differences

The success of reproductive technologies, such as embryo transfer and IVF, greatly depend on the response to superovulation. Using hormonal therapy, it is possible to increase the reproductive rates of beef and dairy cattle for individual and herd treatment. The objective of the present study was to assess the effect of stimulating treatment with FSH or eCG on the oocytes production in Nelore (Bos indicus) donors. The experiment was carried out at Biotec (Cesumar farm, Maringá, Paraná State, Brazil). Forty-two cows that originated from the same genetic group, between 4 and 9 years old were used, with an average weight of 420 kg. The cows received an intramuscular injection of estradiol benzoate (EB, 2 mg) and an ear implant of Norgestomet® (3 mg). Then, they were divided in 3 groups: control group (n = 14); group 1 = eCG treatment (n = 14); and group 2 = FSH treatment (n = 14). In group 2, cows received on 120 IU of FSH on Days 5 and 6, divided in 4 doses that were administrated at 12-h intervals. On Day 7, all implants were removed and ovum pick-up (OPU) was performed. The results were statistically analyzed by Proc GLM (version 8.0, SAS Institute, Cary, NC, USA) and the mean differences were analyzed by Tukey test. Of 42 OPU sessions, a total of 627 oocytes were recovered, of which 502 were viable and 125 were degenerated, but there were no significant differences (P > 0.05) between the groups, as shown in Table 1. We conclude that the gonadotrophic stimulations with FSH or with eCG used in this study did not increase the overall quality of the viable oocytes. More research is needed to determine the optimal ovarian stimulation protocol in Nelore cows. Table 1.Number of oocytes recovered after OPU session in cows that were superstimulated with FSH or eCG1

229 EVALUATION OF OOCYTE QUALITY RECOVERED FROM Gir BREED COWS WITH SYNCHRONIZED FOLLICULAR WAVE ON IN VITRO EMBRYO PRODUCTION

2011 ◽  
Vol 23 (1) ◽  
pp. 213
Author(s):  
L. T. Iguma ◽  
J. N. S. Sales ◽  
R. I. T. P. Batista ◽  
M. M. Pereira ◽  
C. C. R. Quintão ◽  
...  
Keyword(s):  
Cumulus Cell ◽  
Personal Communication ◽  
Fertilization In Vitro ◽  
Embryo Production ◽  
Follicular Waves ◽  
Follicular Wave ◽  
Cell Layers ◽  
The Mean ◽  
In Vitro Embryo Production

Recent data reveal that approximately 80% of bovine embryos produced in vitro worldwide are from (Brazil J. H. M. Viana, personal communication). Adoption of this biotechnology by Brazilian producers might be attributed to particularities of the Bos indicus subspecies. Zebu breeds provide 2 to 3 times more viable oocytes than Bos taurus breeds per ovum pickup (OPU) session. This work aimed to evaluate the quality of cumulus–oocyte complexes (COC) retrieved from Gir breed cows (B. indicus) by OPU with a synchronized follicular wave and subjected to an in vitro embryo production (IVP) technique. All COC were obtained by OPU of 14 Gir cows performed every 14 days. There were 4 OPU sessions, preceded by synchronization of the follicular waves. Wave synchronization was achieved by an injection of 2 mg of oestradiol benzoate followed by the insertion of a norgestomet implant that stayed in place for 5 days. On Day 5, follicles of 2 to 8 mm were aspirated. The oocytes recovered were distributed according to classification: Group 1, with ≥3 cumulus cell layers and homogeneous or slightly heterogeneous cytoplasm (n = 314); and Group 2, ≤2 cumulus cells layers and slightly or coarsely granulated cytoplasm (n = 236). Oocytes from both groups were followed to in vitro maturation–IVF–fertilization–in vitro culture. Two (Day 2) and 8 (Day 8) days after the beginning of fertilization, the cleavage and blastocyst rates were measured, respectively. The statistical analyses were performed using a chi-square test (P < 0.05). The mean cleavage rates observed were 72.93 and 69.07% (not significant), whereas the mean blastocyst rates were 37.90 and 17.80%, and were significantly different for Groups 1 and 2, respectively. The results suggest that the morphology of oocytes (number of cumulus cell layers and cytoplasm homogeneity) is a strong indicator of the in vitro developmental capacity of oocytes recovered from Gir cows with synchronized follicular waves, although we did not observe compromised cleavage rates. Financial support was provided by Fapesp/Fapemig/CNPq/Embrapa. Epamig/Faz. Calciolândia/Nutricell–Nutrientes Celulares is acknowledged.

12 Assessment of oocyte and embryo production in senescent beef cows

2020 ◽  
Vol 32 (2) ◽  
pp. 131
Author(s):  
R. H. Alvarez ◽  
B. M. Bayeux ◽  
Y. F. Watanabe ◽  
D. A. Joaquim ◽  
K. M. R. Duarte ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Prostaglandin F2α ◽  
Beef Cows ◽  
Estradiol Benzoate ◽  
Oocyte Quality ◽  
Wilcoxon Test ◽  
Embryo Production ◽  
Significant Difference ◽  
Intravaginal Device

Fertility associated with age has been poorly documented in cattle. There is evidence that more than 50% of cows became infertile (failure to bear a calf for two successive years) at the age of 15. The causes of infertility in cows have been attributed to poor oocyte quality and/or follicle depletion. The present study aimed to compare the rates of oocyte and invitro embryo production of fertile cows older than 15 years. Sixteen Nellore cows (Bos taurus indicus) 200.3±4.9 months old were submitted to two ovum pickup series (OPU1 and OPU2) at a 2-year (2017 and 2019) interval. Before the OPU1 of each series, the cows were prepared with an IM injection of 2mg estradiol benzoate and 0.15mg prostaglandin F2α analogue and a progesterone-releasing intravaginal device (1g). After five days, the progesterone-releasing intravaginal device was withdrawn and the OPU1 was performed. The second OPU (OPU2) was performed 5 days later to aspirate only the growing follicles. The number and morphologic quality of recovered cumulus-oocyte complexes were registered. After IVM (tissue culture medium 199) and fertilization (Tyrode's solution) of cumulus-oocyte complexes, the presumptive zygotes were cultured in synthetic oviductal fluid with amino acids to assess developmental rates to blastocyst. Data were analysed by chi-squared and Wilcoxon test. The number of oocytes recovered at OPU1 was higher in 2107 than in 2019 (27.8±6.6 vs. 10.8±4.2; P&lt;0.01), whereas in OPU2 the production of oocytes was not different (14.7±3.3 vs. 10.9±3.1). In OPU1, eight cows in 2017 and four in 2019 produced more than 20 oocytes, compared with only one cow in OPU2 of both years (P=0.02). Although there was no significant difference in the proportion of viable oocytes in OPU1 (83.6% and 87.2%) and OPU2 (85.5 and 68.0%), more viable oocytes were produced at OPU1 of 2017 (23.2±5.8) compared with OPU1 of 2019 (9.4±4.1), OPU2 of 2017 (12.6±2.7) and OPU2 of 2019 (7.4±2.4), respectively (P&lt;0.05). There was no significant difference in the proportion of cleaved oocytes in OPU1 (74.2 and 75.1%) and OPU2 (68.9 and 79.8%). Two cows in 2017 (12.5%) and five in 2019 (31.2%) did not produce embryos in both OPUs. Cows produced more embryos (blastocysts) in OPU1 at 2017 (7.3±1.6) than 2019 (2.8±0.6; P&lt;0.05), whereas in OPU2 there was no difference (4.1±0.8 and 3.1±0.8). In conclusion, senescent fertile cows decreases the number of oocytes and embryos as they approach 20 years of age; however, the quality of oocytes does not appear to be compromised. Financial support was provided by FAPESP (proc. 2018/07450-5).

The effects of in vitro maturation duration on maturation rate and intracytoplasmic sperm injection-derived embryonic development in goats

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
A. H. Nor Farizah ◽  
M. M. Rahman ◽  
W. E. Wan Khadijah ◽  
R. B. Abdullah
Keyword(s):  
Embryonic Development ◽  
Intracytoplasmic Sperm Injection ◽  
Cumulus Cell ◽  
Cleavage Rate ◽  
Maturation Rate ◽  
Cell Layers ◽  
Embryo Cleavage

The aim of the present study was to evaluate the effects of <italic>in vitro</italic> maturation (IVM) duration on maturation rate and intracytoplasmic sperm injection (ICSI)-derived embryonic development in goat embryos. Donor goats were superovulated following oestrus synchronisation prior to laparoscopic oocyte pick-up. The quality of oocytes was scored based on cumulus cell layers, which were graded A, B and C. The oocytes were cultured in IVM medium with two different IVM durations (18-21 h and 22-25 h) for the ICSI procedure. A total of 327 matured oocytes were used, of which 157 and 170 oocytes were used for 18-21 h and 22-25 h IVM duration, respectively. The embryo cleavage rate of Grade A from the 18-21 h IVM group was significantly (P<0.05) higher than that of grades B and C. However, in the 22-25 h IVM duration group, the cleavage rates for all grades of oocytes were not significantly (P>0.05) different. Regardless of oocyte grades, no significant differences in maturation rates and cleavage rates for all stages of embryonic development between the two groups of IVM durations were observed. The results suggest that both IVM durations have the same potential in ICSI-derived embryonic development.

scholarly journals A Prospective Intervention Trial on Tailored Radiofrequency Ablation of Uterine Myomas

Medicina ◽  
2020 ◽  
Vol 56 (3) ◽  
pp. 122
Author(s):  
Alessandro Fasciani ◽  
Giovanni Turtulici ◽  
Giacomo Siri ◽  
Simone Ferrero ◽  
Rodolfo Sirito
Keyword(s):  
Quality Of Life ◽  
New Technologies ◽  
Transvaginal Ultrasound ◽  
Safe Procedure ◽  
Ultrasound Guided ◽  
Post Intervention ◽  
Vaginal Ultrasound ◽  
Uterine Myomas

Background and Objective: Investigating the use of radiofrequency myolysis (RFM) for the treatment of fibroids through less invasive access by combining transvaginal ultrasound, hysteroscopy and laparoscopy. Materials and Methods: Fifty-four premenopausal women with 106 symptomatic uterine myomas. Patients underwent RFM in three ways: Vaginal Ultrasound-guided RFM (VU-RFM), Laparoscopic RFM (L-RFM) and Hysteroscopic-RFM (H-RFM). The mean patient age was 43 years; 52 symptomatic uterine myomas were subserosal, 44 intramural and 10 submucosal. The outcomes evaluated at 1 and 12 months after RFM were myoma size (volume-diameter), “Uterine Fibroid Symptom and Quality of Life (UFS-QOL)” questionnaire and a 10-point Visual Analogue Scale (VAS). The therapy was completed with a single ablation in all patients, no complication was registered. The average number of fibroids treated per intervention was two with the use of different accesses: 64/106 VU-RFMs (60.4%), 32/106 L-RFMs (30.2%) and 10/106 H-RFMs (9.4%). Results: Volume and diameter of fibroids were significantly reduced by, respectively, 51.3% and 20.1% in the first 30 days post-intervention (p < 0.001) up to a maximum of 73.5% and 37.1% after the second follow-up visit at 12 months (p < 0.001). A similar trend was shown in terms of disability with a progressive and significant reduction of symptoms (menorrhagia, dysmenorrhea, dyspareunia and pollakiuria) demonstrated by percentage variation of UFS-QOL Symptom Severity and VAS scores to −74.3% and −45.3% as well as −84.9% and −74.3%, respectively, at 1 and 12 months after RFM (p < 0.001). An overall improvement in the quality of life was also demonstrated by a significant increase in the UFS-QOL total score of +38.2% in the first 30 days post-intervention up to +44.9% after the second follow-up visit at 12 months (p < 0.001). The overall average surgery time of the RFM for each patient was 48 minutes, and the time to treat each fibroid by Vaginal Ultrasound-guided RFM (23 min) was found to be significantly less than those of laparoscopy or hysteroscopy (respectively 35 and 34 min) (p < 0.05). An electromagnetic virtual needle tracking system (VNTS) was successfully tested during the RFM procedures, and real-time contrast-enhanced ultrasound (CEUS) has proven to be effective in determining the duration of myolysis through the identification of eventual residual areas of enhancement within the fibroids. Conclusion: Radiofrequency can be considered a minimally invasive and safe procedure for the treatment of uterine myomas through the customization and possible combination of transvaginal, laparoscopic or hysteroscopic accesses. The standardization of the ablation technique with pre-intervention biopsy and new technologies such as VNTS and CEUS spares healthy uterine tissue and may change the future management of symptomatic uterine fibroids.

scholarly journals Supplementation of cows with linseed: effects on antral follicle count, oocyte quality, and nutritional and plasma metabolite parameters

2019 ◽  
Vol 40 (6) ◽  
pp. 2637
Author(s):  
Christopher Junior Tavares Cardoso ◽  
Ana Caroline Bini de Lima ◽  
Wilian Aparecido Leite da Silva ◽  
Mirela Brochado Souza-Cáceres ◽  
Daniela Moraes Pereira ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Antral Follicle ◽  
Oocyte Quality ◽  
Antral Follicle Count ◽  
Neutral Detergent Fiber ◽  
Significant Difference ◽  
Lipid Supplementation ◽  
The Mean ◽  
Plasma Metabolite

Linseed is distinguished by its composition of fatty acids, which are rich in unsaturated acids, and by the slow release of oil in the rumen, consequently providing a greater amount of lipids of interest for intestinal absorption. The aim of this study was to evaluate the effect of linseed supplementation on the antral follicle count (AFC) and oocyte quality of Girolando cows, as well as their digestive parameters and plasma metabolite. Twelve Girolando cows were used in the study and were randomly distributed in two experimental groups: control (CTL, n = 6) and linseed (LINS, n = 6). During the 126 days of supplementation, seven transvaginal ultrasound-guided ovum pick-up (OPU) sessions were performed at intervals of 21 days. AFC was performed in each OPU session. The cows fed with linseed showed no significant difference in the dry matter coefficient of digestibility (63.11 and 62.88), crude protein (62.7 and 55.26), neutral detergent fiber (NDF; 75.36 and 77.15), or acid detergent fiber (ADF; 72.45 and 74.77) for LINS and CTL, respectively. On the contrary, a higher level of ethereal extract was observed for LINS (69.31 vs. 40.7). There was no effect (p > 0.05) of lipid supplementation on plasma metabolite between groups and OPU sessions. There was no difference (p > 0.05) in the mean number of antral follicles (CTL: 31.14 ± 1.97; LINS: 25.52 ± 2.68), cumulus oocyte complexes recovered (CTL: 6.45 ± 1.66; LINS: 5.28 ± 1.18), or oocyte quality (CTL: 60.48% ± 8.46; LINS: 64.54% ± 7.77). The supplementation of 800g of linseed in the diet of Girolando cows did not alter the apparent digestibility of nutrients, AFC, or quality of oocytes obtained by OPU.

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