150 EXAMINATION OF ESTRUS SYNCHRONIZATION USING A PRID® + PROSTAGLANDIN F2α AND PREGNANCY RATE AFTER ARTIFICIAL INSEMINATION FOLLOWING TRANSFER OF IN VITRO-PRODUCED EMBRYOS IN REPEAT-BREEDING COWS

2008 ◽  
Vol 20 (1) ◽  
pp. 155
Author(s):  
M. Urakawa ◽  
A. Ideta ◽  
K. Hayama ◽  
K. Tsuchiya ◽  
Y. Aoyagi
Keyword(s):  
Pregnancy Rate ◽  
Rate Control ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Uterine Horn ◽  
Estrus Synchronization ◽  
Significant Difference ◽  
Clinical Disorders

Repeat-breeding Holstein cows (RBCs), i.e. cows that have undergone three or more AIs, have reduced pregnancy rates. The present study aimed to determine whether application of a combination of a PRID� (ASKA Pharmaceutical Co., Inc., Tokyo, Japan) + prostaglandin F2α (PGF2α) for estrus synchronization and AI + embryo transfer (ET) would improve pregnancy rates in RBCs. All RBCs selected showed a normal interval between the previous insemination and estrus, and were free of clinical disorders. The RBCs (n = 107) received a PRID for 9 days with a capsule containing 10 mg of estradiol benzoate, 0.5 mg IM of cloprostenol (PGF2α, Resipron�-C; ASKA, Japan) at 2 days before PRID removal. The cows were inseminated with frozen–thawed semen from a bull at 0 to 12 h after onset of estrus. Seven days after AI, single in vitro-produced embryos were transferred to the same RBCs. Pregnancy was diagnosed by palpation per rectum at e60 days post-insemination. Exp. 1 estimated the response to estrus synchronization treatment (n = 107). The factors examined were lactation number (mean � SD: 2.3 � 1.5, range: 1–6), insemination number (5.2 � 2.2, range: 3–13), and the interval from calving to next AI (275.9 � 97.6 days, range: 93–650 days). Exp. 2 compared the pregnancy rate in control RBCs (n = 161) and PRID-treated RBCs (n = 100). The factors examined included lactation number (2.5 � 1.6, range: 1–12), insemination number (6.0 � 2.5, range: 3–19), the interval from calving to next AI (288.0 � 102.1 days, range: 102–797 days), and whether the ET was into the uterine horn ipsilateral or contralateral to the CL in a total of 261 RBCs. Data were analyzed using logistic regression. The results for Exp. 1: 103 cows (96.3%) were confirmed to be in estrus (2.0 � 0.3 days after PRID removal). Although the frequency of estrus was affected by the lactation number (P = 0.014) and the interval from calving to the first AI (P = 0.005), the AI number did not have an affect (P = 0.624). Exp. 2: PRID synchronization had no effect on pregnancy rate (control: 37.3%, 60/161; and PRID-treated: 40.0%, 40/100). For the RBCs, the pregnancy rate was not affected by lactation number (P = 0.093), AI number (P = 0.694), or the interval from calving to next AI (P = 0.762). No significant difference (P = 0.428) in pregnancy rate was observed in cows when the embryos were transferred into the uterine horn contralateral to the CL (43.5%, 20/46), compared to those embryos transferred into the uterus horn ipsilateral to the CL (37.2%, 80/215). In conclusion, our findings suggest that the pregnancy rate may be improved by considering lactation number and the interval from calving to the next AI of RBCs in estrus synchronization using a combination of PRID + PGF2α and AI + ET.

scholarly journals COMPARISON OF LONG-TERM PROGESTERONE-BASED PROTOCOL ON REPRODUCTIVE PERFORMANCE OF PREPUBERTAL AND PUBERTAL BEEF HEIFERS.

2014 ◽  
Vol 19 (1) ◽  
Author(s):  
Emanoel Faleiros Silveira ◽  
Luiz Ernandes Kozicki ◽  
Marcio Saporski Segui ◽  
Romildo Romualdo Weiss ◽  
Ivo Walter Santos ◽  
...  
Keyword(s):  
Ultrasound Examination ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Device Removal ◽  
Timed Artificial Insemination ◽  
Red Angus ◽  
Natural Service

The aim of this study was to investigate estrus synchronization in prepubertal and pubertal heifers (crossbred ½ Nelore x Red Angus - commercial herd) submitted to a long-term progesterone-based protocol for timed artificial insemination (TAI), as well as overall pregnancy rates. Four hundred forty-seven heifers underwent ultrasound examination of the ovaries and were assigned to one of 3 groups: (1) prepubertal heifers (PPNS; n = 114) with ovarian follicles <8 mm in diameter and no corpus luteum (CL) that were submitted to natural service (NS) by bulls for 10 days; (2) prepubertal heifers (PPTAI; n = 124) with follicles <8 mm in diameter and no CL that underwent estrus synchronization with injection of 4.0 mg estradiol benzoate (EB) and insertion of a controlled-release intravaginal progesterone (P4) device containing 0.558 g P4 on d 0, followed by P4 device removal and injection of 150 mg prostaglandin F2α and 0.6 mg estradiol cypionate (ECP) on d 8, and TAI on d 10; and (3) pubertal heifers (PTAI; n = 209) with follicles >8 mm in diameter and/or a CL that underwent the same protocol as PPTAI heifers. Overall pregnancy rate was determined by transrectal ultrasonography on d 45 after NS or TAI. Data were analyzed using the FREQ procedure of SAS (SAS Inst. Inc., Cary, NC). Pregnancy rates were as follows: 20.1% in PPNS, 37.0% in PPTAI, and 48.5% in PTAI (P < 0.05). In conclusion, TAI after estrus synchronization with a hormonal protocol using EB and ECP in prepubertal and pubertal heifers resulted in higher pregnancy rates than prepubertal heifers exposed to natural service, and exposure of prepubertal heifers to a long-term progesterone-based protocol effectively synchronized estrus.

108 FACTORS AFFECTING PREGNANCY RATES IN RECIPIENTS RECEIVING IN VITRO-PRODUCED EMBRYOS BY FIXED TIME EMBRYO TRANSFER

2016 ◽  
Vol 28 (2) ◽  
pp. 184
Author(s):  
M. Pelizzari ◽  
A. Tribulo ◽  
J. Garzon ◽  
B. Bernal ◽  
R. Tribulo ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Embryo Transfer ◽  
Body Condition Score ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Uterine Horn ◽  
Device Removal ◽  
Embryo Transfer Technique

A retrospective analysis of factors that affect pregnancy rates from 4214 fresh in vitro-produced (IVP) embryos that were transferred at a fixed-time (FTET) in 20 different farms. Recipients were all cycling cows or heifers that were synchronized with 1 of 3 treatments: 1) treatments with progesterone (P4) devices and 2 mg of oestradiol benzoate (EB) on Day 0 (day of insertion) and 24 h after device removal (Day 8); 2) treatments with P4 devices and EB on Day 0, but with 0.5 mg of oestradiol cypionate (ECP) at device removal (Day 8); or 3) treatments with P4 devices and GnRH on Day 0 and a second GnRH 60 h after device removal (Day 5). Cows in all treatment groups also received 500 µg of cloprostenol (prostaglandin F2α) at the time of P4 device removal and 400 IU of eCG either at device removal or 3 days before device removal. All embryos were transferred 7 or 8 days after the expected time of oestrus (24 h after EB, 48 h after ECP or at the time of the second GNRH for each synchronization treatment, respectively). On the day of embryo transfer, recipients were examined by ultrasonography and those with corpus luteum >14 mm in diameter received a fresh, IVP embryo in the uterine horn ipsilateral to the corpus luteum. Pregnancy rates were determined by ultrasonography 35 days after FTET. Data were analysed by logistic regression. Independent variables were classified into the following three categories. 1) Factors related to the recipient and the environment; there were no significant differences in pregnancy rates for corpus luteum diameter (≥14 and <16 mm, ≥16 and <18 mm, or ≥18 mm; P = 0.46), number of corpus luteum (1 or ≥2; P = 0.26), and category of recipient (cow or heifer; P = 0.21). However, there were significant effects of farm (P = 0.01) and body condition score (BCS; P = 0.01). Cows with BCS ≥4.5 (1 to 5 scale) resulted in lower pregnancy rates (4/20, 20.0%) than those with BCS 2 (74/225, 32.9%), 2.5 (502/1434, 35.0%), 3 (570/1467, 38.9%), 3.5 (193/532, 36.3%), and 4 (44/118, 37.3%). 2) Factors related to the synchronization treatment; there were no significant differences between recipients receiving eCG at device removal (84/209, 40.2%) or 3 days before device removal (874/2291, 38.1%; P = 0.35). However, recipients synchronized with P4 devices and ECP had higher (P = 0.01) pregnancy rates (232/483, 48.0%) than those treated with EB (679/1888, 36.0%) or gonadotropin-releasing hormone (47/129, 36.4%). 3) Factors related to the embryo transfer technique; day of the recipient’s oestrous cycle (P = 0.36), stage of embryo transferred (IETS stages 6 or 7; P = 0.62), and operator (P = 0.57) did not affect pregnancy rates. However transfers made in the anterior third of the uterine horn resulted in higher (649/1545, 42.0%) pregnancy rates than those in the mid-third (845/2511, 33.6%) or in the distal third (6/35, 17.1%; P = 0.01). It was concluded that factors related to the recipient and the environment (farm and BCS), the synchronization treatment (ECP), and the embryo transfer technique (site of deposition) affect pregnancy rates in recipients of embryos produced in vitro and transferred at a fixed time.

17 INFLUENCE OF DIFFERENT CONCENTRATIONS OF PROGESTERONE DURING FIXED-TIME ARTIFICIAL INSEMINATION PROTOCOLS ON OVULATION AND PREGNANCY RATES IN NELORE HEIFERS

2008 ◽  
Vol 20 (1) ◽  
pp. 89
Author(s):  
M. F. Pegorer ◽  
R. L. Ereno ◽  
C. M. Barros
Keyword(s):  
Artificial Insemination ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
High Concentration ◽  
Serum Progesterone ◽  
Significant Difference ◽  
Intravaginal Device

Previous reports indicate that a high concentration of serum progesterone (P4) decreases LH pulse frequency (Burke et al. 1996 Anim. Reprod. Sci. 45, 13–28) and, consequently, follicular growth. This may reduce the efficiency of fixed-time artificial insemination (FTAI) protocols that utilize P4 in Bos indicus heifers (Marques et al. 2005 Acta Sci. Vet.). The objective of the present work was to evaluate the influence of different P4 concentrations on the ovulation and pregnancy rates of zebu heifers subjected to hormonal protocols for FTAI. Four hormonal protocols with different P4 concentrations were used to induce synchronization of ovulation in Nelore (Bos indicus) heifers (n = 292). Before the beginning of the treatments, the animals were subjected to ovary evaluations by ultrasonography (Aloka SSD 500, 5-MHz probe; Aloka, Tokyo, Japan) twice in an interval of 10 days. Only heifers with a corpus luteum (CL) in at least one of the ultrasonographic evaluations were utilized. At a random stage of the estrous cycle, the selected heifers received an intravaginal progesterone-releasing device containing 0.558 g of P4 [Triu�, Biogenesis, Buenos Aires, Argentina; Treatment 1 (T1); n = 76], or 1 g of P4 [Cronipress�, Biogenesis; Treatment 2 (T2); n = 70], or 0.558 g of P4 and prostaglandin F2α (PGF2α) [150 �g d-cloprostenol, IM, Croniben�, Biogenesis; Treatment 3 (T3), n = 75], or 1 g of P4 and PGF2α [Treatment 4 (T4), n = 71]. At the time of intravaginal device insertion (Day 0 = D0), all animals received 2 mg of estradiol benzoate (EB, Bioestrogen�, IM, Biogenesis), and PGF2α was administered only in animals from groups T3 and T4. Eight days later (D8), PGF2α (0.150 g d-cloprostenol) was administered to all heifers and the P4 source was removed. Twenty-four h after removal of the intravaginal device, the heifers were treated with EB (1 mg, IM), and 30 to 36 h later all animals were fixed-time inseminated (FTAI, D10), without estrus detection. In a subset of heifers from each group (T1 = 28; T2 = 34; T3 = 33; T4 = 31), ovarian ultrasonography was performed on D9 (at 8:00 A.M.) and D11 (at 14:00 P.M.) in order to determine ovulation rate after each treatment. The data were analyzed by logistic regression (GENPROC, SAS; SAS Institute, Inc., Cary, NC, USA). Ovulation rates after treatments T1, T2, T3, and T4, were, respectively, 71.4% (20/28), 76.5% (26/34), 84.9% (28/33), and 70.9% (22/31), and there was no significant difference among groups (P > 0.05). Pregnancy rates, ascertained by ultrasonography 40 days after AI, were 30.2% (23/76), 25.7% (18/70), 33.3% (25/75), and 28.2% (20/71), respectively, for T1, T2, T3, and T4, and likewise there was no significant difference among groups (P > 0.05). It is concluded that reduction of progesterone concentration in the intravaginal device (1 g v. 0.558 g) associated or not with administration of PGF2α at the beginning of treatments did not significantly influence ovulation and pregnancy rates in Nelore heifers submitted to a FTAI protocol. Additional experiments are underway to confirm or not the present results. This work was supported by FAPESP (Brazil) and BIOGENESIS (Argentina).

213 THE EFFECT OF eCG TREATMENT IN AN ESTRUS SYNCHRONIZATION PROTOCOL ON PREGNANCY RATES AFTER EMBRYO TRANSFER IN HOLSTEIN HEIFERS

2007 ◽  
Vol 19 (1) ◽  
pp. 223
Author(s):  
T. Okazaki ◽  
E. Sasaki ◽  
K. Hasegawa ◽  
T. Takani ◽  
S. Abe
Keyword(s):  
Embryo Transfer ◽  
Prostaglandin F2α ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Control Group ◽  
Corpora Lutea ◽  
Estrus Synchronization ◽  
Chi Square ◽  
Synchronization Protocol

Recent studies have shown that the presence of accessory or multiple corpora lutea (CL) and increased progesterone (P4) concentrations reduced early embryonic mortality in cattle. The objective of this study was to evaluate the effect of equine chorionic gonadotropin (eCG) treatment on the number of CL, the P4 concentrations, and pregnancy rates after embryo transfer (ET). Holstein heifers (n = 120) from 7 dairy farms received an intravaginal progesterone-releasing device (CIDR; InterAg, Hamilton, New Zealand) and 2 mg IM of estradiol benzoate (EB; Gynandol®; Sankyo, Tokyo, Japan) at random stages of the estrous cycle. After 7 to 9 days, CIDRs were removed and 15 mg of prostaglandin F2α (PG; Pronalgon®; Pfizer Japan, Nagoya, Japan) were administered, followed by 100 µg IM GnRH (Conceral®; Takeda Pharmaceutical Co., Ltd., Osaka, Japan) 2 days later (Day 0). The heifers were placed at random into 3 groups for eCG treatment. The eCG was not administered in a control group (n = 53); heifers in other 2 groups received 1000 IU eCG (Peamex®; Sankyo, Japan) IM at the time (0 h group, n = 37) or 48 h before (48 h group, n = 30) PG injection/CIDR removal. On Day 7, heifers were examined by ultrasonography (Aloka SSD500; Aloka, Tokyo, Japan) for number of CL; heifers with at least one functional CL received an in vivo-derived frozen–thawed embryo by direct transfer. At the same time, a blood sample was collected to determine P4 concentration. Pregnancy rates were determined on Days 30 and 60 by ultrasonography and rectal palpation, respectively. The data were analyzed by ANOVA and means were compared with Fisher's PLSD. Proportional data were analyzed by the chi-square test. P4 concentrations (mean ± SD) on Day 7 were 1.8 ± 1.0, 5.6 ± 3.3, and 2.2 ± 1.1 ng mL−1 for the control, 48 h, and 0 h groups, respectively (48 h vs. control and 0 h; P &lt; 0.001). The number of CL on Day 7 were 1.1 ± 0.4, 2.5 ± 1.4, and 1.8 ± 0.9 for the control, 48 h, and 0 h groups, respectively (control vs. 48 h and 0 h, and 48 h vs. 0 h; P &lt; 0.01). Pregnancy rates did not differ between 0 and 48 h groups but both were higher than in the control group (Table 1). Results suggest that the estrus synchronization protocol with administration of eCG at the time of CIDR removal or 48 h earlier significantly increased the number of CL and the P4 concentration, and improved pregnancy rates in Holstein heifers after ET. Table 1.Pregnancy rates of Holstein heifers synchronized with CIDR and PG and treated with eCG

scholarly journals 218 Effect of GnRH injection at -72 h in MGA-PG estrus synchronization protocol

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 122-122
Author(s):  
McKay Erickson ◽  
Rick N Funston
Keyword(s):  
Pregnancy Rate ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Estrus Synchronization ◽  
Estrus Detection ◽  
To Receive ◽  
Observation Period ◽  
Synchronization Protocol

Abstract Beef heifers (n = 1524) from two operations in central Nebraska were randomly assigned to one of two treatments [0 or 5 μg GnRH at prostaglandin F2α (PGF) administration 72 hrs before insemination]. Both locations utilized MGA-PG fixed-time AI (TAI) estrus synchronization protocol. The first location (L1, n = 1076, 382 ± 3 kg) processed heifers 72 hours prior to insemination and every third heifer was assigned to receive an injection of GnRH (5 ug) (TRT) and an injection of PGF. The remaining heifers received PGF and 0 μg GnRH. At insemination all heifers received 100 ug GnRH. Heifers were then observed for estrus behavior from 10–21 days post TAI and re-inseminated if estrus was detected. Heifers pregnant from the second breeding were added to final pregnancy rate. The second location (L2) utilized estrus detection patches and performed only TAI (n = 448, 363 ± 7 kg) following MGA-PG synchronization protocol outlined above without the estrus observation period. Treatment did not significantly (P > 0.20) improve TAI pregnancy rates [L1 TAI 56.3% (TRT) vs 57.3%; L1 AI Total 78.3% (TRT) vs 73.9%; L2 TAI 58.5% (TRT) vs 52.5%] among the two herds. The administration of 5 μg GnRH at PGF tended to increase (P = 0.12, 74% vs 63%) pregnancy rates for those inseminated during the follow up heat detection period at L1. The treatment did not (P > 0.20) improve pregnancy for the time-AI heifers. In addition, TAI pregnancy rates were similar (P > 0.20) at L2. There were greater (P < 0.20) effect of pen on pregnancy rate.

189 PREGNANCY RATES IN HEAT-STRESSED DAIRY CATTLE RECEIVING ONE OR TWO IN VITRO-PRODUCED EMBRYOS IN A TIMED EMBRYO TRANSFER PROGRAM

2006 ◽  
Vol 18 (2) ◽  
pp. 202
Author(s):  
M. Franco ◽  
J. Block ◽  
F. D. Jousan ◽  
L. A. de Castro e Paula ◽  
A. M. Brad ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Fetal Loss ◽  
Pregnancy Rates ◽  
Uterine Horn ◽  
Limiting Factor ◽  
Transfer Program ◽  
In Pregnancy

The objective was to determine whether transfer of two embryos would increase pregnancy rates in heat-stressed dairy recipients receiving an in vitro-produced embryo transferred into the uterine horn ipsilateral to the corpus luteum (CL). Such a treatment would increase the likelihood that the cow receives at least one embryo competent for sustained development. In addition, transfer of two embryos into the ipsilateral uterine horn is likely to increase the amounts of interferon-tau and other embryonic-signaling molecules in the uterus. A total of 32 virgin crossbreed heifers and 26 lactating crossbreed cows were used as timed embryo transfer recipients after being subjected to an ovulation synchronization protocol as follows: GnRH (100 �g) and insertion of previously used progesterone-containing CIDR on Day -10, prostaglandin F2� and CIDR removal on Day -3, and GnRH (100 �g) on Day 0 (day of anticipated ovulation). All recipients had a palpable CL on Day 6 and were randomly selected to receive one (n = 31 recipients) or two (n = 27) embryos on Day 7. At Day 64, the pregnancy rate tended to be higher (P = 0.07) for cows than for heifers. While not significant, heifers that received two embryos tended to have lower pregnancy rates than those that received a single embryo (20% vs. 41%); there was no difference in pregnancy rate in cows (50% for two embryos vs. 57% for one embryo). Pregnancy losses between Day 64 and Day 127 occurred in one group only cows receiving two embryos. In that group, pregnancy rate was 50% at Day 64 but 17% at Day 127. Overall, there was no difference in pregnancy rates at day 127 between cows and heifers, but recipients that received two embryos (17% for cows and 20% for heifers) had lower pregnancy rates (P < 0.03) than recipients that received one embryo (57% for cows and 41% for heifers). Only one animal, a cow, had twin fetuses at day 127. In conclusion, unilateral transfer of two embryos failed to improve pregnancy rates of dairy cattle exposed to heat stress. The fact that fetal loss occurred sooner for heifers than for cows points out the importance of uterine capacity as a limiting factor for maintenance of fetal development of two conceptuses. The suitability of timed embryo transfer was evident from the high pregnancy rates achieved with crossbreed females that received a single embryo. This work was supported by BARD Grant No. US-1551-14, USDA TSTAR Grant No. 2004-14135-14715, Grant No. 2001-12101-11318 from the USDA-IFAFS Program, and CAPES Grant No. 134202-1).

P–131 Significance of the phenomenon of blastomere exclusion from compaction: Its relation to irregular cleavage, blastocyst development rate, and pregnancy rate

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Watanabe ◽  
M Tomida ◽  
S Suzuki ◽  
Y Matsuda ◽  
K Yoshikai ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Chromosome Analysis ◽  
Development Rate ◽  
Pregnancy Rates ◽  
Blastocyst Transfer ◽  
Blastocyst Development ◽  
Miscarriage Rate ◽  
Single Blastocyst Transfer ◽  
Significant Difference ◽  
Irregular Cleavage

Abstract Study question When does blastomere exclusion from compaction increase and what effect does it have on the embryo? Summary answer More blastomere were excluded from compaction in embryos with irregular cleavage, resulting in lower blastocyst development rates, but no decrease in pregnancy rates at transfer. What is known already It has been reported that many of the chromosome analysis results of blastomere excluded from compaction were aneuploid, and pointed out that this exclusion may be related to the repair of blastocyst euploidy, but the effect of the number of excluded blastomere has not been reported. Study design, size, duration This is a retrospective study of 578 embryos that developed into morula with time-lapse monitoring by EmbryoScope (Vitrolife) in 2018–2019. Participants/materials, setting, methods The target embryos were classified into two groups: embryos with normal first and second cleavage (normal cleavage group) and embryos with irregular cleavage (dynamics of one cell dividing into three or more cells), called “direct cleavage”, at either cleavage (DC group), and the number of blastomere excluded from compaction during morula formation was recorded and compared. The blastocyst development rate and single blastocyst transfer pregnancy rates of the two groups were compared. Main results and the role of chance There are 286 in the normal cleavage group and 292 in the DC group. The mean number of excluded blastomere was 0.76 and 3.55, respectively, which was significantly higher in the DC group (P &lt; 0.01). Good blastocyst (Gardner classification 4 or higher) development rate was 84.5% (239/283) and 65.8% (181/275), respectively, and high grade blastocyst (Gardner classification BB or higher) development rate was 43.9% (105/239) and 14.9% (27/181) of them, both significantly higher in the normal cleavage group (P &lt; 0.01). The single blastocyst transfer pregnancy rates were 31.6% (25/79) and 32.4% (11/34), and the miscarriage rates were 24.0% (6/25) and 27.3% (3/11), respectively, neither was there a significant difference between the two groups. So, direct cleavage increased the number of blastomere excluded from compaction, decreased the rate of morula to good blastocyst development and reduced blastocyst grade, but did not affect blastocyst transfer pregnancy rate and miscarriage rate. Limitations, reasons for caution Please note that all target embryos must have developed into morula or larger (embryos that did not develop into morula will not be included in the study). Wider implications of the findings: Severe chromosomal aberrant blastomeres formed by direct cleavage were excluded from compaction, and the blastocyst development rate decreased due to a decrease in the amount of viable cells, but it is suggested that this blastomere exclusion mechanism is not related to euploidy after blastocyst development. Trial registration number Not applicable

scholarly journals The Effect of A PRID®Delta-Based Estrus Synchronization Protocol On Pregnancy Rates In Cows With No Estrus Symptoms

2022 ◽  
Author(s):  
Murat Can Demir ◽  
Cihan Kaçar ◽  
Umut Çağın Arı ◽  
Semra Kaya ◽  
Oğuz Merhan ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Pregnancy Rates ◽  
Dominant Follicle ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Follicle Size ◽  
Equine Chorionic Gonadotropin

Abstract The present study aimed to determine the effects of progesterone-based gonadotropin-releasing hormone (GnRH), prostaglandin F2α (PGF2α), and equine chorionic gonadotropin (eCG) injections on progesterone profiles and pregnancy rates in cows with no estrus symptoms within 60 days after parturition. A total of 80 cows were included in the study. All animals had the progesterone-releasing device PRID®Delta placed intravaginally for nine days with an injection of GnRH. On the eighth day, PGF2α was injected, and PRID®Delta was removed from the vagina on day nine. Artificial insemination was carried out 60 hours after PRID®Delta removal. In half of the animals (n = 40), 600 IU of eCG was injected when PRID®Delta was removed on the ninth day before artificial insemination 60 hours later. Blood samples were taken from the tail vein on days 0 and 8 to determine progesterone levels. The pregnancy rate in the group that received eCG was 37.5%, while it was 27.5% in those that did not (P = 0.4). While the dominant follicle diameter was 15.5 mm in cows injected with eCG during timed artificial insemination, the follicle diameter was 12.4 mm in cows with no eCG injection (P <0.001). There were no differences in serum progesterone values in blood samples taken until the time of artificial insemination. However, progesterone values in the blood taken during artificial insemination were 0.94 ng/ml in the eCG- group and 0.72 ng/ml in the eCG+ group (P <0.05). As a result, it was determined that eCG injections, in addition to progesterone-based GnRH and PGF2α applications, increased the pregnancy rates in cows without symptoms of estrus. The dominant follicle diameter was larger in cows treated with eCG during artificial insemination; however, follicle size did not increase the pregnancy rate.

scholarly journals Comparative Study Between Mock Embryo Transfer Prior To The Treatment Cycle and Real Embryo Transfer In In Vitro Fertilization

KnE Medicine ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Hilma Putri Lubis
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Treatment Cycle ◽  
Uterine Cavity ◽  
Pregnancy Rates ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Significant Difference ◽  
Clinical Pregnancy Rates

<p><strong>Introduction</strong><strong></strong></p><p>A trial or mock embryo transfer (ET) may influence pregnancy rates and it performed prior to ET allows the clinician to assess the uterine cavity and the utero-cervical angle. The aim of this study is to compare the consistency of the type of ET in mock ET with real ET.</p><p><strong>Material &amp; Methods</strong></p><p>A retrospective comparative analysis of  patients who underwent in vitro fertilization or ICSI cycle from January 2014 to December 2014 in Halim Fertility Center was done. The type of transfer was divided into two groups: ‘easy’ or ‘difficult’. An easy ET was defined as a transfer that occurred without the use of manipulation or other instrumentation and difficult ET was considered when additional instrumentation was required.</p><p><strong>Results</strong></p><p>From the study, 103 patients who underwent Mock-ET, we  found 58 patients (56.3%) with easy ET and 45 patients (43.7%) with difficult ET, which with hard catheter ET in 17 patients (16.5%), with osfander assistance in 20 patients (19.4%) and with stylet in 8 patients (7,8%). 58 patients with Easy Mock ET group were entirely easy real ET (100%) and 45 patients with difficult Mock ET group also entirely were difficult real ET (100%). The Statistical analysis shows no significant difference between the mock ET and real ET groups (p&gt;0,05). In easy real ET, clinical pregnancy rates were 32.8% and in difficult real ET, clinical pregnancy rates were 26.7% with no significant difference between the  groups (p&gt;0,05).</p><p><strong>Conclusion:</strong></p><p>Mock ET prior to the treatment cycle is consistent with real ET.</p>

scholarly journals The effectiveness of collaborative infertility counseling (CIC) on pregnancy outcome in infertile women undergoing in vitro fertilization: A randomized controlled trial

2019 ◽  
Author(s):  
Mahboobeh Rasoulzadeh Bidgoli ◽  
robab latifnejad roudsari ◽  
ali montazeri
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Treatment Success ◽  
Intervention Group ◽  
Control Group ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Significant Difference ◽  
And Control

Abstract Background: Infertility is an emotional tension which influences the whole aspects of relationships in infertile couples. A main objective of infertility treatments is elevation of pregnancy rate. The present study aimed to examine the effect of collaborative counseling on pregnancy rate in infertile women, undergoing in vitro fertilization in Mashhad, Iran. Methods: In this clinical trial, 60 women with primary infertility were selected from an infertility research center and were randomly allocated into intervention (n=29) and control (n=31) groups. The intervention group received individual counseling, based on the collaborative reproductive healthcare model with collaboration of a midwife, a gynecologist and a clinical psychologist in five sessions during a two-month period. The control group received routine care. Positive pregnancy test was considered as a criterion of treatment success at the end of the study. Data were analyzed using statistical tests including independent samples t-test. Results: There was no significant difference in pregnancy rate between intervention and control groups (P = 0.298). Also, there were no significant differences in follicle and embryo numbers between two groups. However, a significant difference was observed between two groups in terms of oocyte numbers where the intervention group had more oocyte (P = 0.014). Conclusion: Overall the findings indicated that the collaborative infertility counseling did not improve treatment success in infertile women undergoing in vitro fertilization

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