76 SCREENING Oct-4 PROMOTER ACTIVITY IN BOVINE FIRST AND SECOND ROUND SCNT EMBRYOS USING AN EGFP REPORTER CONSTRUCT

2006 ◽  
Vol 18 (2) ◽  
pp. 146 ◽  
Author(s):  
A. Wuensch ◽  
F. A. Habermann ◽  
R. Klose ◽  
V. Zakhartchenko ◽  
F. Yang ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Promoter Activity ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Transcript Level ◽  
Max Planck ◽  
Pluripotent Cells ◽  
Gene Construct ◽  
Reporter Gene Construct ◽  
Fetal Fibroblasts

Oct-4, also known as Oct-3 or POU5F1, belongs to the POU (Pit-Oct-Unc) transcription factor family. So far it has been identified in mice, cattle, pigs, and humans. In mice, pluripotent cells of the early pre-implantation embryo express Oct-4. Therefore, Oct-4 expression is considered to be a marker for pluripotency in mice. In bovine blastocysts, Oct-4 protein is not restricted to the pluripotent cells of the inner cell mass (ICM) but is also present in the trophectoderm (TE). It has been reported, however, that at this stage the transcript level of Oct-4 was already downregulated in the TE suggesting a similar regulation of Oct-4 transcription in bovine and mice. To obtain insight into the regulation of the Oct-4 promoter after somatic cell nuclear transfer (SCNT), we transfected bovine fetal fibroblasts (BFF) with GOF18-�PE-EGFP, a reporter gene construct for the Oct-4 promoter (kindly provided by Dr. Hans R. Schoeler, Director, Max Planck Institute for Molecular Biomedicine, M�nster, Germany). Six stably transfected colonies (BFFGOF), none of which exhibited green fluorescence, were used for SCNT, with subsequent examination of the resulting embryos on Days 5-7 by fluorescence microscopy. SCNT embryos originating from BFF colonies 3, 12, and 82 showed fluorescence both in ICM and TE. SCNT with BFF colony 8 resulted in embryos with no detectable fluorescence. This might have been due to a positional effect of the reporter gene construct or an incomplete reprogramming of the used fibroblast colonies during SCNT. To study the consequences of another round of SCNT on Oct-4 promoter activity, cloned embryos from BFF colonies 12 (positive) and 8 (negative) were transferred to recipients. At Day 33, three BFFGOF12 and four BFFGOF8 fetuses were recovered and BFF cultures were established from all cloned fetuses. The presence of GOF18-�PE-EGFP was verified by PCR and Southern blot analysis. BFF cultures from two cloned BFFGOF12 and BFFGOF8 fetuses each were used for SCNT, and fluorescence was examined in Day 6 embryos. SCNT embryos derived from BFFGOF12 fetuses exhibited weaker fluorescence than embryos directly derived from the original transfected colony. SCNT embryos derived from BFFGOF8 fetuses showed no fluorescence. GFP-positive embryos will be examined by immunocytochemistry using an antibody against Oct-4 to evaluate the correlation between endogenous Oct-4 and Oct-4 reporter gene expression. Further fluorescence in situ hybridization (FISH) analyses are underway to localize the reporter gene integration sites in BFFGOF8 and BFFGOF12. In summary, stable GOF18-�PE-EGFP transfected cells are an interesting tool for monitoring Oct-4 promoter activation after using different protocols of SCNT or in consecutive rounds of SCNT. Furthermore, it will be possible to correlate Oct-4 promoter activity with epigenetic mechanisms, such as DNA methylation and histone modifications, in SCNT embryos.

38 RABBIT NUCLEAR TRANSFER AND IN VIVO-FERTILIZED EMBRYOS FAIL TO EXPRESS A MOUSE Oct-4 PROMOTER-DRIVEN EGFP REPORTER GENE

2007 ◽  
Vol 19 (1) ◽  
pp. 137 ◽  
Author(s):  
R. Hao ◽  
A. Wuensch ◽  
R. Klose ◽  
E. Wolf ◽  
V. Zakhartchenko
Keyword(s):  
Dna Methylation ◽  
Fluorescence Microscopy ◽  
Reporter Gene ◽  
Nuclear Transfer ◽  
Donor Cell ◽  
Bovine Embryos ◽  
Egfp Gene ◽  
Gene Construct ◽  
Fetal Fibroblasts

Reprogramming of a donor cell genome during somatic cell nuclear transfer (SCNT) is largely dependent on appropriate expression of 'pluripotency'? genes, such as Oct-4 (POU5F1). Recently, we transfected bovine fetal fibroblasts with GOF18-ΔPE-EGFP, a reporter gene construct for the Oct-4 promoter and assessed the expression of Oct-4 after SCNT (Wuensch et al. 2006 Reprod. Fertil. Dev. 18, 144). Our previous study on DNA methylation reprogramming revealed that rabbit in vivo-fertilized and cloned embryos differ from bovine embryos in respect to this epigenetic modification (Shi et al. 2004 Biol. Reprod. 71, 340–347), suggesting differences in the mechanism of epigenetic reprogramming between these two species. In this study, we tested whether GOF18-ΔPE-EGFP could be used to monitor Oct-4 expression in rabbit cloned embryos. The reporter gene construct included the EGFP gene flanked by a 9-kb fragment of the murine Oct-4 upstream region with a deletion in the proximal enhancer (PE) and a 9-kb fragment containing the nontranscribed murine structural Oct-4 gene. The 21.2-kb fragment GOF18-DPE-EGFP was released from the vector backbone by NotI digestion and purified with QIAquickGel Extraction Kit (Qiagen, Hilden, Germany) after gel electrophoresis. Four stable transfected colonies of rabbit fetal fibroblasts (RFF), none of which exhibited green fluorescence, were used for SCNT. The resulting embryos were examined on Days 2–5 by fluorescence microscopy. To detect endogenous Oct-4 expression, in vivo-fertilized embryos were stained with anti-mouse Oct-3 antibody and then incubated with secondary Alexa 488-conjugated goat anti-mouse antibody. The most prominent endogenous Oct-4 expression was detected in in vivo-fertilized embryos at the morula and blastocyst stages. Depending on the donor cell line used for nuclear transfer, cleavage and blastocyst rates ranged from 56 to 97% and from 33 to 49%, respectively. When a total of 230 cloned embryos at the 2-to 16-cell stages and 93 cloned morulae and blastocycts were examined by fluorescence microscopy, none of the examined embryos exhibited fluorescence signals indicating the lack of Oct-4 promoter activity. Taking into account the fact that both cloned and in vivo-fertilized rabbit embryos have specific patterns of DNA methylation reprogramming, which are different from that of bovine embryos, we injected GOF18-ΔPE-EGFP gene constructs into pronuclei of in vivo-fertilized zygotes. None of the 74 injected embryos, which were examined at the 2-cell to blastocyst stages, showed fluorescence signals. Our results demonstrate that rabbit nuclear transfer and in vivo-fertilized embryos are unable to activate a mouse Oct-4 promoter-reporter construct. Potential reasons include incompatibilities between mouse Oct-4 promoter sequences and rabbit transcription factors as well as specific mechanisms of epigenetic reprogramming in the rabbit. This work was supported by the Bayerische Forschungsstiftung.

scholarly journals A type I collagen reporter gene construct for protein engineering studies. Functional equivalence of transfected reporter COL1A1 and endogenous gene products during biosynthesis and in vitro extracellular matrix accumulation

1993 ◽  
Vol 293 (2) ◽  
pp. 387-394 ◽  
Author(s):  
S R Lamandé ◽  
J F Bateman
Keyword(s):  
Extracellular Matrix ◽  
Reporter Gene ◽  
Type I Collagen ◽  
Type I ◽  
Wild Type ◽  
Reporter Construct ◽  
Reporter Protein ◽  
Gene Construct ◽  
Reporter Gene Construct

A type I collagen reporter gene construct, designed to facilitate detailed analysis of the consequences of introduced structural and regulatory mutations on collagen biosynthesis and participation in the extracellular matrix, was produced by site-directed mutagenesis of the mouse COL1A1 gene. The reporter construct, pWTCI-Ile822, carried a single base change which converted the codon for amino acid 822 of the triple helix from methionine to isoleucine. This change allowed the reporter protein, [Ile822]alpha 1(I), to be distinguished from the wild-type alpha 1(I), and quantified, by its altered CNBr cleavage pattern. In mouse Mov13 cells, which synthesize no endogenous pro alpha 1(I), reporter chains associated with endogenous pro alpha 2(I), formed pepsin-stable triple helices and were secreted efficiently from the cell. The thermal stability of wild-type molecules and molecules containing the reporter [Ile822]alpha 1(I) chains was identical. The biosynthetic characteristics of wild-type and reporter chains were directly compared in stably transfected 3T6 cells. These cells did not make a distinction between reporter and endogenous alpha 1(I) chains, which were secreted from the cells at the same rate and were processed and deposited into the 3T6 cell in vitro accumulated extracellular matrix with equal efficiency. These data demonstrate that the helical sequence alteration in the reporter protein is functionally neutral and that the reporter construct, pWTCI-Ile822, is a suitable vector for the analysis of the biochemical effects of site-directed mutations in the putative COL1A1 functional domains.

Identification, isolation and culture of pluripotent cells from the porcine inner cell mass

1996 ◽  
Vol 113 (1-6) ◽  
pp. 427-436 ◽  
Author(s):  
M. Ropeter-Scharfenstein ◽  
N. Neubert ◽  
K. Prelle ◽  
W. Holtz
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Pluripotent Cells

scholarly journals 45PRODUCTION OF CHIMERIC TRANSCHROMOSOMIC CALVES

2004 ◽  
Vol 16 (2) ◽  
pp. 144
Author(s):  
P. Kasinathan ◽  
M.F. Nichols ◽  
J.E. Griffin ◽  
J.M. Robl
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Peripheral Blood Lymphocytes ◽  
Blastocyst Stage ◽  
Human Artificial Chromosome ◽  
Fish Analysis ◽  
Blastocyst Development ◽  
Cell Stage ◽  
Fetal Fibroblasts

Chimeras have been used for investigating fundamental aspects of early embryonic development, and differentiation, and for introducing foreign genes into mammals (Robertson et al., 1986 Nature 323, 445–448; Cibelli et al., 1998 Science 280, 1256–1258). The main objective of this study was to determine if the transfer of blastomeres from in vitro-produced (IVP) embryos into cloned, transchromosomic embryos improved the efficiency of producing transchromosomic calves. Cloned embryos were produced using in vitro-matured bovine oocytes and bovine fetal fibroblasts containing a human artificial chromosome (HAC) (Kuroiwa et al., 2002 Nat Biotechnol 20, 889–894). IVP embryos were produced using standard procedures and blastomeres were harvested at the 8–16 cell stage by removing the zona pellucida with protease. Cloned embryos were randomly divided on Day 4 into two groups. One group received 3–4 IVP blastomeres while a second group served as a control (nonmanipulated cloned embryos). After transferring the blastomeres, the chimeric and cloned embryos were placed in culture (Kasinathan et al., 2001 Biol. Reprod. 64, 1487–1493) and on Day 7 development to the blastocyst stage was evaluated. Grades 1 and 2 embryos were transferred; two each per synchronized recipient. Pregnancy maintenance, calving, and calf survival were evaluated in both groups. Presence of a HAC in live calves was evaluated in both fibroblasts and peripheral blood lymphocytes (PBLs) using FISH analysis. Embryo development to the blastocyst stage, maintenance of pregnancy and number of calves born were analyzed using Chi-square. There were no differences in the rate of blastocyst development at day 7 or establishment of pregnancy at 40d (P>0.05). However, pregnancy rate at 120d, and number of calves that developed to term and were alive at birth (chimera 14/54 and clone 4/90), and at 1 month of age (chimera 13/54 and clone 1/90) were lower (P<0.01) for cloned embryos. The proportion of cells containing an HAC in PBLs, was higher in cloned calves (100%) compared to chimeric calves (26%). The HAC retension rates in PBLs in HAC-positive chimeric and cloned calves were 84% and 95%, respectively. These data indicate that, although the proportion of calves retaining an HAC was lower in chimeras compared to clones, more HAC-positive calves were produced in the chimeric treatment from fewer cloned embryos. We speculate that higher rates of development in the chimeras may be related to the normality of the placenta. Future studies will be required to determine the contribution of the IVP blastomeres to both the inner cell mass and trophectoderm. Therefore, a chimeric approach may be useful for improving the efficiency of producing cloned transchromosomic calves.

Developmental complexity of early mammalian pluripotent cell populations in vivo and in vitro

1998 ◽  
Vol 10 (8) ◽  
pp. 535 ◽  
Author(s):  
T. A. Pelton ◽  
M. D. Bettess ◽  
J. Lake ◽  
J. Rathjen ◽  
P. D. Rathjen
Keyword(s):  
Cell Biology ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Types ◽  
Experimental Manipulation ◽  
Pluripotent Cell ◽  
Pluripotent Cells ◽  
Cellular Origin

Early mammalian embryogenesis is characterised by the coordinated proliferation, differentiation, migration and apoptosis of a pluripotent cell pool that is able to give rise to extraembryonic lineages and all the cell types of the embryo proper. These cells retain pluripotent differentiation capability, defined in this paper as the ability to form all cell types of the embryo and adult, until differentiation into the three embryonic germ layers at gastrulation. Our understanding of pluripotent cell biology and molecular regulation has been hampered by the difficulties associated with experimental manipulation of these cells in vivo. However, a more detailed understanding of pluripotent cell behaviour is emerging from the application of molecular technologies to early mouse embryogenesis. The construction of mouse mutants by gene targeting, mapping of gene expression in vivo, and modelling of cell decisions in vitro are providing insight into the cellular origin, identity and action of key developmental regulators, and the nature of pluripotent cells themselves. In this review we discuss the properties of early embryonic pluripotent cells in vitro and in vivo, focusing on progression from inner cell mass (ICM) cells in the blastocyst to the onset of gastrulation.

53 EFFECTS OF INSULIN-TRANSFERRIN-SELENIUM IN DEFINED AND PORCINE FOLLICULAR FLUID-SUPPLEMENTED MEDIUM ON IN VITRO PRODUCTION OF PORCINE SCNT EMBRYOS

2007 ◽  
Vol 19 (1) ◽  
pp. 144
Author(s):  
Y. U. Kim ◽  
D. P. Bhandari ◽  
M. S. Hossein ◽  
S. M. Park ◽  
E. Lee ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Defined Medium ◽  
Culture Conditions ◽  
Developmental Competence ◽  
Differential Staining ◽  
In Vitro Production ◽  
Fetal Fibroblasts

Insulin promotes the uptake of glucose and amino acids, and is beneficial for maturation of oocytes in vitro. Transferrin is an iron-transport protein and selenium is an essential trace element. Insulin-transferrin-selenium (ITS) together has been used in some in vitro maturation systems. The present study was designed to evaluate the effects of ITS in defined and porcine folicular fluid (pFF)-supplemented IVM medium on the glutathione (GSH) concentration, and on developmental competence after somatic cell nuclear transfer. ITS liquid media supplement (I-3146) was purchased from Sigma-Aldrich (St Louis, MO, USA). Basic IVM medium was TCM-199 supplemented with 10 ng mL-1 epidermal growth factor, 4 IU mL-1 pregnant mare serum gonadotropin (PMSG) and hCG and either 1% PVA (defined medium) or 10% pFF. Ten �g mL-1 insulin, 5.5 �g mL-1 transferrin, and 5 �g mL-1 selenium was used for the entire 44-h culture period. The GSH content of a gruop of 10 to 20 oocytes was determined by the dithionitrobezoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. Fetal fibroblasts were used as somatic cell donors and reconstructed embryos were cultured in mNCSU-23 medium for 168 h. Cleavage and blastocyst formation was observed at 48 h and 168 h, respectively. The quality of blastocysts was assessed by differential staining of the inner cell mass (ICM) and the trophectoderm (TE) cells. Each experiment was replicated for 5 times. The data were analyzed by one-way ANOVA, and Tukey was used as a posthoc test. The level of GSH production significantly varied in different culture conditions. The highest GSH concentration was observed in the pFF + ITS group (8.2 picomol/oocyte). A total of 116, 125, 126, and 120 reconstructed oocytes were cultured, and 10.1, 15.3, 17.2, and 21.8% blastocysts were observed for PVA, PVA + ITS, pFF, and pFF + ITS groups, respectively (P < 0.05). The numbers of inner cell mass, trophrectoderm cells, and total cells were significantly higher in the pFF + ITS group compared with the other groups. The average number of total cells in blastocysts was 31.9 � 1.8, 43.1 � 3.5, 46.7 � 4.9, and 52.3 � 6.7 for PVA, PVA + ITS, pFF, and pFF + ITS groups, respectively (P < 0.05). ITS supplement improved the developmental competence in both the defined and the pFF supplemented groups. We recommend supplementing porcine IVM medium with 10 �g mL-1 insulin, 5.5 �g mL-1 transferrin, and 5 �g mL-1 selenium.

53 TARGETED SCREEN FOR AMINO ACIDS THAT REGULATE BOVINE INNER CELL MASS DEVELOPMENT

2016 ◽  
Vol 28 (2) ◽  
pp. 156
Author(s):  
V. Najafzadeh ◽  
R. Martinus ◽  
B. Oback
Keyword(s):  
Inner Cell Mass ◽  
Statistical Significance ◽  
Cell Mass ◽  
Positive Control ◽  
Drop Out ◽  
Blastocyst Development ◽  
Acetyl Coa ◽  
Cell Numbers ◽  
Fetal Fibroblasts

Pluripotency relies on species-specific amino acid (AA) metabolism. In the mouse, inner cell mass (ICM) and ICM-derived pluripotent stem cells (PSCs) need threonine, which is catabolized by threonine dehydrogenase (TDH) into acetyl–CoA and glycine. Depleting (Δ) the culture medium of threonine (ΔT) or blocking TDH activity induces PSC death. By contrast, human PSCs do not survive without lysine (ΔK), leucine (ΔL), or methionine (ΔM). Since isolated bovine PSCs cannot be propagated in vitro, we screened for AAs that selectively support pluripotent ICM cells in intact bovine embryos. Five days (D5) post-IVF, embryos were transferred into glutamine-free synthetic oviduct fluid (gSOF) with Eagle’s nonessential (NE) and essential (E) AAs (gSOF_AA) plus BSA. Embryos were individually cultured until D8 under different conditions. Statistical significance was determined using Fisher’s exact test for blastocyst development (morphological grading to IETS standard) and t-tests for cell numbers (differential stain) and gene expression (quantitative or qPCR). Removal of BSA reduced grade 1–3 blastocyst (B1–3) development (37% v. 25%, n = 3; P < 0.001). Depleting NEAAs from gSOF_AA did not significantly decrease B1–3, but depleting all 12 EAAs did (25% v. 8%, n = 6; P < 0.001). Because ΔEAA was most effective, we focused on this. Experiments were conducted in gSOF+NEAA and compared with gSOF_AA as a positive control (n = 2–6 replicates). One (ΔT, ΔM), two (ΔMT, ΔCM, ΔCT; ΔIL, ΔIK, ΔKL), three (ΔCMT, ΔIKL), or six (ΔHPRVWY) EAA drop-out did not affect blastocyst formation, even when NEAAs were also removed for ΔT and ΔM groups (n = 3). However, depleting another six (ΔCIKLMT), nine (+CMT, +IKL), or eleven EAAs (+T, +M) increasingly compromised B1–3 (P < 0.05). Because no clear EAA candidates emerged from the screen, we focused on TDH. TDH mRNA was present at similar levels in microsurgically isolated (by microblade) trophectoderm (TE) and chemically isolated (by Triton X-100) ICM, but undetectable in five adult tissues. Despite ΔT medium showing no effect, exposure to the TDH inhibitor QC1 (50 µM) reduced B1–3 and B1–2 compared with a dimethylsulfoxide (DMSO) solvent control (25% v. 37% and 8% v. 19%, n = 8; P < 0.005). ICM and TE cell numbers were equally reduced in QC1 v. DMSO-treated blastocysts (10 v. 19 and 37 v. 67 with N = 21 and N = 29 embryos, respectively, n = 3; P < 0.005). Yet TDH, hypoblast (PDGRFα), epiblast (NANOG, FGF4, SOX2), and trophoblast (CDX2, KRT8) markers were not consistently affected by QC1. We next applied 3-hydroxynorvaline (3-HNV), which TDH hydrolyses into glycine and propionyl-CoA instead of acetyl-CoA. Compared with solvent controls, 3-HNV (300 µM) killed all embryos and bovine fetal fibroblasts within 3 days in ΔT medium. This toxic effect was fully rescued by >10-fold T-supplementation. Thus, 3-HNV protein incorporation, rather than acetyl-CoA reduction, may nonspecifically impair cellular function. In summary, we found that bovine ICM formation did not specifically depend on metabolizing threonine or any other single EAA. Research was supported by AgResearch Core Funding.

145. TRP53 REGULATES THE FORMATION OF A PLURIPOTENT INNER CELL MASS IN THE EARLY EMBRYO

2009 ◽  
Vol 21 (9) ◽  
pp. 63
Author(s):  
L. Ganeshan ◽  
C. O'Neill
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Blastocyst Stage ◽  
Early Embryo ◽  
Pluripotent Cells ◽  
Drug Induced ◽  
Developmental Potential

The developmental viability of the early embryo requires the formation of the inner cell mass (ICM) at the blastocyst stage. The ICM contributes to all cell lineages within the developing embryo in vivo and the embryonic stem cell (ESC) lineage in vitro. Commitment of cells to the ICM lineage and its pluripotency requires the expression of core transcription factors, including Nanog and Pou5f1 (Oct4). Embryos subjected to culture in vitro commonly display a reduced developmental potential. Much of this loss of viability is due to the up-regulation of TRP53 in affected embryos. This study investigated whether increased TRP53 disrupts the expression of the pluripotency proteins and the normal formation of the ICM lineage. Mouse C57BL6 morulae and blastocysts cultured from zygotes (modHTF media) possessed fewer (p < 0.001) NANOG-positive cells than equivalent stage embryos collected fresh from the uterus. Blocking TRP53 actions by either genetic deletion (Trp53–/–) or pharmacological inhibition (Pifithrin-α) reversed this loss of NANOG expression during culture. Zygote culture also resulted in a TRP53-dependent loss of POU5F1-positive cells from resulting blastocysts. Drug-induced expression of TRP53 (by Nutlin-3) also caused a reduction in formation of pluripotent ICM. The loss of NANOG- and POU5F1-positive cells caused a marked reduction in the capacity of blastocysts to form proliferating ICM after outgrowth, and a consequent reduced ability to form ESC lines. These poor outcomes were ameliorated by the absence of TRP53, resulting in transmission distortion in favour of Trp53–/– zygotes (p < 0.001). This study shows that stresses induced by culture caused TRP53-dependent loss of pluripotent cells from the early embryo. This is a cause of the relative loss of viability and developmental potential of cultured embryos. The preferential survival of Trp53–/– embryos after culture due to their improved formation of pluripotent cells creates a genetic danger associated with these technologies.

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