365 THE EFFECT OF ADMINISTERING PROGESTERONE AND ESTRADIOL PRIOR TO eFSH ON THE SUPEROVULATORY RESPONSE OF MARES

2006 ◽  
Vol 18 (2) ◽  
pp. 290 ◽  
Author(s):  
N. Logan ◽  
P. McCue ◽  
M. Alonso ◽  
E. Squires

eFSH has been used to induce multiple ovulation in cycling mares. However, the response to eFSH is variable. Generally, eFSH is initiated 5 to 7 days after ovulation at a time when the follicular population on the ovaries may be variable. The objective of this study was to determine whether administration of progesterone and estradiol for 10 days prior to eFSH (Bioniche Animal Health, Athens, GA) would enhance the response to eFSH administration. Thirty normal cycling mares were assigned to 1 of 2 groups. Group 1: Control eFSH treatment - mares were examined daily with ultrasonography beginning 5 days after ovulation. Once the follicles in these mares reached 20 to 25 mm in size, eFSH treatment (12.5 mg, i.m.) was administered twice a day. Cloprostenol (Schering-Plough, Union, NJ, USA) treatment (250 �g) was administered on the second day of eFSH treatment. eFSH treatment continued until the majority of the cohort of follicles reached e35 mm. Treatment was stopped and after approximately 36 h hCG (2500 IU, i.v.; Chorulon; Intervet, Millsboro, MD, USA) was administered. Group 2: Injectable progesterone + estradiol (150 mg of progesterone and 10 mg of estradiol; P+E) treatment was initiated in diestrus (5 to 7 days post-ovulation) for 8 mares and in early estrus for 7 mares in this group. Injectable progesterone was continued for 10 days and Cloprostenol (250 �g) was administered on Day 10. Mares were then examined daily with ultrasonography and, once they had acquired 20- to 25-mm follicles, eFSH treatment was initiated. Twice-daily injections of eFSH (12.5 mg, i.m.) were continued until a majority of the cohort of follicles was e35 mm. hCG was administered approximately 36 h later. All mares were inseminated with 1 billion progressively motile spermatozoa from one of two stallions on the day of hCG administration and on the following day with cooled semen (1 billion progressively motile spermatozoa) from the same stallion. Data were analyzed by t-test and Fisher's Exact Test. The number of days of eFSH treatment was similar for the P+E (2) vs. the control (1) group (4.2 � 2.0 vs. 4.9 � 1.3 days, respectively). However, the number of ovulations induced in response to eFSH was greater for mares in the eFSH control (1) group (5.6 � 2.0) than for those in the P+E (2) group (3.0 � 1.9). Embryo recovery per flush was also greater for eFSH control (1) mares (2.7) vs. P+E (2) mares (1.1). Embryo quality was excellent or good for all embryos in both groups. Seventy-three percent of the mares (11 of 15) in both groups gave at least one embryo at each recovery attempt. However, more mares in the eFSH control (1) group gave two or more embryos (60%) compared to those in the P+E (2) group (20%). In summary, treatment of mares with P+E prior to eFSH treatment resulted in fewer ovulations, fewer embryos recovered, and fewer mares providing e2 embryos. Thus, there was no advantage in pretreating mares with P+E prior to eFSH treatment.

2010 ◽  
Vol 128 (1) ◽  
pp. 5-9 ◽  
Author(s):  
Marcelo Soares Privado ◽  
Adriana Machado Issy ◽  
Vera Lucia Lanchote ◽  
João Batista Santos Garcia ◽  
Rioko Kimiko Sakata

CONTEXT AND OBJECTIVE: Controversy exists regarding the site of action of fentanyl after epidural injection. The objective of this investigation was to compare the efficacy of epidural and intravenous fentanyl for orthopedic surgery. DESIGN AND SETTING: A randomized double-blind study was performed in Hospital São Paulo. METHODS: During the postoperative period, in the presence of pain, 29 patients were divided into two groups: group 1 (n = 14) received 100 µg of fentanyl epidurally and 2 ml of saline intravenously; group 2 (n = 15) received 5 ml of saline epidurally and 100 µg of fentanyl intravenously. The analgesic supplementation consisted of 40 mg of tenoxicam intravenously and, if necessary, 5 ml of 0.25% bupivacaine epidurally. Pain intensity was evaluated on a numerical scale and plasma concentrations of fentanyl were measured simultaneously. RESULTS: The percentage of patients who required supplementary analgesia with tenoxicam was lower in group 1 (71.4%) than in group 2 (100%): 95% confidence interval (CI) = 0.001-0.4360 (P = 0.001, Fisher's exact test; relative risk, RR = 0.07). Epidural bupivacaine supplementation was also lower in group 1 (14.3%) than in group 2 (53.3%): 95% CI = 0.06-1.05 (P = 0.03, Fisher's exact test; RR = 0.26). There was no difference in pain intensity on the numerical scale. Mean fentanyl plasma concentrations were similar in the two groups. CONCLUSION: Intravenous and epidural fentanyl appear to have similar efficacy for reducing pain according to the numerical scale, but supplementary analgesia was needed less frequently when epidural fentanyl was used. CLINICAL TRIAL REGISTRATION NUMBER: NCT00635986


2005 ◽  
Vol 17 (2) ◽  
pp. 232
Author(s):  
M.F.G. Nogueira ◽  
J. Buratini Jr ◽  
C.M. Barros

The LH receptor (LHr) present in ovarian follicle cells is essential for the biological response due to its binding to the ligand (LH). The events following the LH surge are related to the presence of LHr and its affinity to LH. Studies on LHr gene expression in theca and granulosa cells from bovine follicles have demonstrated the presence of at least four isoforms of LHr mRNA by alternative splicing. Two of the four detected isoforms have an open reading frame (ORF) and can be translated to functional proteins (receptors coupled with G protein) with different affinities to their ligands (Robert C et al. 2003 Reproduction 125, 437–446). From those two isoforms with ORF, one (full) has affinity to both LH and hCG molecules, whereas the second isoform (with deletion of exon 10) has affinity to hCG only. Recently we have detected the same four isoforms described by Robert et al. in both granulosa and theca cells from abattoir. Based on this information, the present study tested the hypothesis that, in cows superstimulated with FSH, administration of both LH and hCG, as an attempt to stimulate any variety of LH receptor present in the follicles, would result in improvement of oocyte quality and/or increase in ovulation rate. Nelore cows (Bos taurus indicus) were superstimulated according to the protocol termed P-36 (Barros CM et al. 2003 Theriogenology 59, 524 abst), and embryos were flushed 7 to 8 days after inducing ovulation. Ovulation was induced with LH (pLH, 12.5 mg, i.m.; Lutropin, Vetrepharm, London, Ontario, Canada; Group 1) or both LH (12.5 mg) and hCG (1500 IU, i.m.; Choragon, Ferring GmbH, Kiel, Germany; Group 2). Superstimulation protocol and embryo transfer were performed simultaneously on both groups. Mean (± SEM) of total structures, viable embryos, and viability rate were: 12.4 ± 2.36, 10.0 ± 2.38 and 80.8% (Group 1, n = 8 flushings) and 12.2 ± 2.03, 8.9 ± 1.66, and 73.1% (Group 2, n = 14); there was no significant difference between groups (P = 0.96; P = 0.71 (ANOVA), and P = 0.18 (Fisher's Exact Test), respectively). In a subset of embryos (excellent, good, fair, and poor qualities) transferred without freezing, the pregnancy rates for groups 1 and 2 were 41.7% (25/60) and 56.9% (37/65), respectively (P = 0.11, Fisher's Exact Test). The overall pregnancy rates from embryos of excellent, good, and fair qualities were 46.3% (25/54) and 58.9% (33/56), respectively for Groups 1 and 2 (P = 0.25). When only embryos of good, fair, and poor qualities were pooled, the pregnancy rates were 18.8% (06/32) and 60.0% (30/50), respectively for Groups 1 and 2 (P = 0.0003). It is concluded that simultaneous administration of LH and hCG to induce ovulation in superstimulated animals did not alter production of viable embryos or viability rate. However, the use of hCG in addition to LH administration could be beneficial for embryos of good, fair, and poor qualities. Nogueira has a fellowship from FAPESP (São Paulo).


Author(s):  
I Putu Eka Jaya ◽  
Nyoman Mangku Karmaya ◽  
Made Jawi ◽  
Wayan Weta ◽  
Ida Bagus Ngurah ◽  
...  

Physical fitness is ability to perform the activity or activities without feeling tired excessively. Physical fitness associated with a person`s organs to carry out its tasks properly every day without experiencing significant fatigue. In a child’s mental retardation with a les mobile lifestyle that will affect his physical fitness. The purpose of this study to determine which type of gymnastic exercise better to the physical fitness on the students in Sekolah Dasar Luar Biasa Kuncup Bunga Denpasar.This research used experimental method using Randomized study Pre and Post Test Group Design. The subjects in this study were students in Sekolah Dasar Luar Biasa Kuncup Bunga Denpasar. There were 18 students which is divided into two groups: group-1 is senam penguins and group-2 is senam ria anak Indonesia. Data were obtained after a six-week training analyzed by paired t-test and unpaired t-test.The results of physical fitness test before training with Senam Penguins was 23.39 ± 1.69 and the test results after the training was 27.87 ± 3.28. the physical fitness test in group-2 before training with Senam Ria anak Indonesia was 22.69 ± 1.76 and the results after training was 24.64 ± 2.11. Paired t-test in both group before and after training showed significant differences after training in group-1 and group-2 with a value of p > 0.05 with an increase in physical fitness test results of (19.15) in group-1 and (8.64) in group-2. The percentage increase physical fitness test results of group-1 with senam penguins is higher than those in group-2 with senam ria anak Indonesia.So we can conclude that the training of senam penguin in Sekolah Dasar Luar Biasa Kuncup Bunga Denpasar improved the physical fitness test results higher than senam ria anak Indonesia.


2004 ◽  
Vol 16 (2) ◽  
pp. 208
Author(s):  
C. Cuello ◽  
F. Berthelot ◽  
F. Martinat-Botté ◽  
P. Guillouet ◽  
V. Furstoss ◽  
...  

The present study was designed to determine the effect of pooling embryos from two donors on the reproductive success of transfer of vitrified/warmed porcine blastocysts. Superovulated Large White hyperprolific gilts (n=24) were used as embryo donors. Gilts were artificially inseminated 12 and 24h after initial detection of estrus using fresh semen, and slaughtered on Days 5.5 to 6 of the estrous cycle (Day 0=Onset of estrus). Embryos were recovered by flushing the uterine horns, and unhatched blastocysts were selected. Vitrification and warming were performed as reported previously (Berthelot et al., 2000 Cryobiology 41, 116–124). Embryo transfers were conducted in asynchronous (−24h) Meishan gilts (n=20). Twenty vitrified/warmed blastocysts were surgically transferred into one uterine horn. Ten recipients received embryos from one donor (group 1) and the other ten transfers were performed with mixed embryos from two donors (group 2). Pregnancy was assessed ultrasonographically at Day 25 after estrus and recipients were slaughtered five days later. The pregnancy rate from the different groups was compared using Fisher exact test. The GLM procedure of SAS was used to determine the effect of the origin of embryos (one or two donors) on the number of developed fetuses and viable fetuses at Day 30 of pregnancy. The ovulation rate was 32.5±11.8 (mean±SD). The total number of embryos collected was 634, of which 57 (9.0%), 36 (5.7%), 513 (80.9%) and 28 (4.4%), were unfertilized oocytes and degenerated embryos, morulae, unhatched blastocysts and hatched blastocysts, respectively. The ratio of collected embryos to the number of corpora lutea was 81.3%. The pregnancy rate for group 1 (70%) was not different (P>0.05) than that for group 2 (90%). No significant differences were detected between group 1 and group 2 for in vivo embryo development (number fetuses/transferred embryos in pregnant recipients; 33.3% v. 40%) or in vivo embryo survival (number viable fetuses/transferred embryos in pregnant recipients; 27.9% v. 33.9%). However, the in vivo efficiency (number viable fetuses/total transferred embryos) was higher (P<0.05) when transfers were performed with embryos from two donors (19.5% v. 30.5%). These results indicate that pooling embryos from two donors increases the in vivo efficiency after transfer of vitrified/warmed porcine blastocysts. This study was supported by grant from SENECA (FPI/99, Spain).


2007 ◽  
Vol 20 (4) ◽  
pp. 673-684 ◽  
Author(s):  
J. Ponti ◽  
B. Munaro ◽  
M. Fischbach ◽  
S. Hoffmann ◽  
E. Sabbioni

The Balb/c3T3 cell transformation assay (CTA) is an available in vitro system to detect the carcinogenic potential of chemicals. Currently, the European Centre for the Validation of Alternative Methods (ECVAM) is validating this test, assessing its reliability and relevance. Its endpoint is the formation of type III foci, which is, when using clone A31-1-1, a very rare event that usually does not occur at all for negative controls. The carcinogenic potential of a compound tested is assessed by comparing the number of foci in treated and untreated cells. The objective of the present work is to optimise the data analysis for this endpoint by applying the most commonly used approach by a t-test and the Fisher's exact test as an alternative approach. For this purpose selected metal compounds classified as carcinogenic (NaAsO2, CdCl2 cisPt), as suspected carcinogenic (C6H5)4AsCl, CH3HgCl), or as compounds without evidence of carcinogenic properties in humans ((NH4)2PtCl6, NaVO3) as well as a non-carcinogenic (AgNO3) were analysed. Our evaluation revealed that the t-test approach, which assumes normality of data, is not appropriate. The results demonstrated that the statistical analysis by Fisher's exact test better reflects the data properties and greatly facilitates the interpretation of Balb/c3T3 CTA data regarding carcinogenic potential.


2018 ◽  
Vol 6 (2) ◽  
pp. 37
Author(s):  
Made Kristira Yanti ◽  
Ni Luh Nopi Andayani ◽  
I Putu Adiartha Griadhi

ABSTRACTHeadache can be caused by increased tension in upper trapezius muscle. This headache is characterized bytrigger point and upper trapezius muscle tautband. The purpose of this study was to compare the positional releasetechnique and infrared interventions with contract relax stretching and infrared on the reduction of headache due toupper trapezius muscle tension. This study was experimental with pre and post-test group design designs of 24 peopledivided into two groups. Group 1 received positional release technique and infrared, while Group 2 received contractrelax stretching and infrared. The result of data analysis with paired sample t-test in Group 1 showed the average 2,242± 0,329 and p = 0,001, while Group 2 showed the average 2,225 ± 0,201 and p = 0,001. From the results of the analysisshowed in each group there is a significant reduction of pain. Based on independent samples test t-test between groups1 and 2 obtained p value = 0.882 (p> 0.05). It can be concluded that the positional release technique and infraredintervention has no significant difference as contract relax stretching and infrared to decreased headache due to uppertrapezius muscle tension.Keywords: Headache, upper trapezius muscle, positional release technique, contract relax stretching, infrared, VAS


2015 ◽  
Vol 06 (04) ◽  
pp. 536-540 ◽  
Author(s):  
S. R. Chandra ◽  
Thomas Gregor Issac ◽  
Mariamma Philip ◽  
A. Krishnan

ABSTRACT Introduction: Autoimmune encephalitis is a group of treatable noninfective encephalitic disorders with great clinical implications. They have a close resemblance to prion disease and some slow virus infections. We report the presence of significant titers of antimeasles antibody in some of our patients with autoimmune encephalitis resulting in diagnostic and therapeutic problems. Patients and Methods: Patients seen by us in the last 4 years with high titers (1:625 dilution) cerebrospinal fiuid (CSF) antimeasles antibody positivity were reviewed retrospectively. The data collected were assessed using SPSS- Statistical Package for Social Sciences Version 15.0 (IBM corporation) software. The groups which showed elevated antimeasles antibody titers but did not have other parameters suggestive of subacute sclerosing panencephalitis (Group 2) were segregated and compared with those who had the typical features (Group 1) using Fisher's Exact Test. Results: There were 33 patients with antimeasles antibody in CSF. Group 1 had 27 and Group 2 had 6 patients. Group 1 had lower age, cognitive dysfunction, slow myoclonus, less generalized tonic-clonic seizures, and focal seizures. Group 2 patients belonged to the higher age, had significant psychosis (P = 0.02), incontinence of bowel and bladder (P = 0.0001). Slow myoclonus was significant in the first group (P = 0.028), and weakness was significant in the second group (P = 0.028) and double incontinence in the second group (P = 0.0001). Magnetic resonance imaging showed significant gray matter and cerebellar involvement in Group 2 P = 0.005 and P = 0.028, respectively. Conclusions: Patients who show significant titers of antimeasles antibodies in the CSF but belonging to older age group with psychosis, generalized tonic-clonic seizures, double incontinence, focal myoclonus, and electroencephalographic and imaging noncorroborative need to be investigated for autoimmune encephalitis in view of the great prognostic and therapeutic relevance.


Author(s):  
Merinda Ulfa ◽  
Ketut Tirtayasa ◽  
Wahyuddin ◽  
Susy Purnawati ◽  
Luh Putu Ratna Sundari ◽  
...  

Background: Disorder of dynamic balance is often found in children with Down syndrome which causes difficulty in maintaining posture against impending disorders. If this is left it will certainly cause problems of further motor development. Purpose: To prove the physiotherapy intervention that can improve dynamic balance among children with down syndrome 7-15 years. Methods: The research is true experimental with pre-test and post-test group design. This study used 24 subjects in 2 groups, group 1 (n=11) given dynamic neuromuscular stabilization on while group 2 (n=11) given perceptual motor program. Divided randomly into 2 groups that is group 1 dynamic neuromuscular stabilization and group 2 perceptual motor program. The exercise is held 3 times a week in 6 weeks. Measurements of this dynamic balance are using sixteen balance tests. Result: The paired sample t-test statistical test in group 1 showed a significant difference with value (p <0.001) on mean before intervention 48.5±5.428 and after intervention 72.75±4.413. The paired sample t-test statistical test in group 2 showed a significant difference with value (p <0.001) on mean before intervention 48.5±5.3 and after intervention 69.42±4.379. Different test between group 1 and 2 using independent sample t-test showed a significant difference with value (p<0.001). Conclusion: The conclusions are dynamic neuromuscular stabilization is better than perceptual motor program in improving dynamic balance among children with down syndrome 7-15 years at Pradnyagama Foundation Denpasar.


2018 ◽  
Vol 1 (1) ◽  
pp. 129-136
Author(s):  
Badereddin Mohamad Al-Ali ◽  
Emma Persad ◽  
Andreas Lunacek ◽  
Christof Mrstik ◽  
Eugen Plas

Introduction: Many studies suggest that varicoceles are associated with hypogonadism and varicocele repair can increase testosterone levels and improve erectile function.Aim: The aim of this retrospective study was to analyze the impact of varicocele and varicocele surgery on testosterone level, semen quality, and erectile function.Methods: Our study included 265 infertile males with a clinical varicocele. This group was divided into three groups: group 1 (193) patients who did not receive surgery, group 2 (72 patients) who were operated on according to the Palomo procedure and group 3 (28 patients), who acted as a control group without a varicocele. All patients completed the International Index of Erectile Function IIEF-5 (German version) and underwent semen analysis. Serum testosterone, follicle stimulating hormone (FSH), and luteinizing hormone (LH) were measured at inclusion into the study and after surgery.Main outcome measures: Changes in semen quality, erectile function, and serum hormones after surgery.Results: The IIEF-5 scores in groups 1 and 2 were 21.01 ± 2.2; and 21.74 ± 1 respectively, and the resulting t-test for equality of variance was significant (p < 0.0001). Total testosterone level in groups 1 and 2 were 3.16 ± 0.37 and 3 ± 0.01, respectively, and the resulting t-test for equality of variance was significant (p < 0.0001). The results of the semen analysis were better in group 2 (after surgery) (28.6%, p < 0.001) in comparison to group 1. Interestingly, pre-operative serum testosterone levels were lower in patients with later improvement of semen analysis (p = 0.05). Body mass index (p = 0.8), pre-operative serum FSH (p = 0.9), LH (p = 0.2), and nicotine consumption (p = 0.6) were similar in both the group that saw improvement and the group with no change in semen quality.Conclusion: Semen quality improved in 28.6% of patients after varicocele surgery. Erectile dysfunction (ED) improved after varicocele surgery. We report that lower pre-operative serum testosterone level might be a possible indicator for successful surgical outcome.


2021 ◽  
Vol 2 (1) ◽  
pp. 10-15
Author(s):  
I Putu Astrawan ◽  
I Putu Prisa Jaya

Background: Footwork training is important in coaching basic movements in badminton. This research aims to learn about the influence of footwork training on improving leg muscles strength. Methods: The study used a randomized pretest-posttest control group design. The participants consisted of 42 male badminton players of Universitas Pendidikan Ganesha, Bali, Indonesia, who split into two groups of footwork training (ten repetitions two sets (group 1) and five repetitions with four sets (group 2)). The coach gave the footwork exercise three times per week for 6 weeks to each group and measured the leg muscle strength using the dynamometer test. Results: The result of the within-group paired T-test before and after the training shows group 1 and group 2 (p<0.05). For group 1, the mean of leg muscle strength was 114.19±33.13 kg before the training and 183.19±33.56 kg after the training, with an increase of 60% (69 kg). On the other hand, for group 2, the mean score of leg muscle strength before the training was 113.05±31.30 kg and after the training was 141.10±34.91, with an increase of 25% (28 kg). The leg muscle strength difference between the groups before and after the training was tested using the independent T-test with a significant α=0.05. Comparing the differences of leg muscle strength for both the groups before the training was p= 0.90, and after the training was p=0.00. Conclusion: The conclusion is training badminton footwork ten repetition two sets better than five repetitions four sets to improve leg muscle strength.


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