222 IN VITRO FERTILIZATION OF OOCYTES OBTAINED THROUGH TRANSVAGINAL OOCYTE RETRIEVAL FROM CYCLIC MURRAH BUFFALOES (BUBALUS BUBALIS)

2006 ◽  
Vol 18 (2) ◽  
pp. 219 ◽  
Author(s):  
R. S. Manik ◽  
M. S. Chauhan ◽  
V. Gupta ◽  
S. K. Singla ◽  
P. Palta
Keyword(s):  
In Vitro Fertilization ◽  
In Vitro Maturation ◽  
Oocyte Retrieval ◽  
Cumulus Cells ◽  
Blastocyst Stage ◽  
Limited Information ◽  
Vitro Fertilization ◽  
Murrah Buffaloes ◽  
Transvaginal Oocyte Retrieval

Very limited information is available in the literature on in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) of oocytes collected through the transvaginal oocyte retrieval (TVOR) technique in buffaloes. Therefore, the present study was undertaken to examine the post IVF cleavage rates and embryonic development up to the blastocyst stage in the Murrah breed of buffalo subjected to TVOR. The five cyclic Murrah buffaloes were synchronized for estrus by a single prostaglandin injection. The animals were subjected to TVOR once weekly for seven weeks. TVOR was performed using an ultrasound machine with a transvaginal convex transducer (5 MHz), a needle guide, a single-lumen 19-gauge 60-cm-long needle, and a vacuum pressure of 50 mmHg. The number and size of follicles in each ovary was determined before puncture. The follicles were characterized on the basis of their diameter as small (3-5 mm), medium (6-9 mm), and large (e10 mm). The oocytes recovered were classified as grade A, cumulus-oocytes complexes with e5 layers of cumulus cells; grade B, those with two to four layers; grade C, partially denuded oocytes; and grade D, completely denuded oocytes. IVM, IVF and IVC were carried as reported by Chauhan et al. (1999 J. Dairy Science 82, 918-926). Briefly, the oocytes were cultured for 24 h in a CO2 incubator (5% CO2 in air) at 38.5�C for in vitro maturation. Frozen-thawed semen was used in Bracket and Olyphant medium for capacitation and fertilization. The in vitro-fertilized and cleaved embryos were cultured further for 9 days in modified synthetic oviductal fluid. The small follicles constituted a major proportion (60%) of the total observed follicles, although a substantial proportion of medium (19%) and large (21%) follicles were also present. A total of 76 oocytes were recovered by aspiration of 110 follicles, with an overall recovery rate of 70% (range 67-74%). Of these, 45 (59%) were of grades A and B, and 31 (41%) were of grades C and D. The mean number of total follicles and the oocytes recovered per session did not differ significantly among individual donors. Out of the 37 oocytes subjected to IVM and IVF, 19 (51%) cleaved at Day 2 post-insemination. A total of four embryos (11%) developed into morulae/blastocysts. This study demonstrates the use of TVOR as a mean of obtaining oocytes, their fertilization, and further embryo development in the Murrah breed of buffalo.

Thrombocythemia and hemoperitoneum after transvaginal oocyte retrieval for in vitro fertilization

2004 ◽  
Vol 82 (3) ◽  
pp. 735-737 ◽  
Author(s):  
Salem A. El-Shawarby ◽  
Raul A. Margara ◽  
Geoffrey H. Trew ◽  
Michael A. Laffan ◽  
Stuart A. Lavery
Keyword(s):  
In Vitro Fertilization ◽  
Oocyte Retrieval ◽  
Vitro Fertilization ◽  
Transvaginal Oocyte Retrieval

Development of pelvic abscess during pregnancy following transvaginal oocyte retrieval and in vitro fertilization

2012 ◽  
Vol 164 (1) ◽  
pp. 116-117 ◽  
Author(s):  
George Patounakis ◽  
Kerry Krauss ◽  
Sara S. Nicholas ◽  
Jason K. Baxter ◽  
Norman G. Rosenblum ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Oocyte Retrieval ◽  
Pelvic Abscess ◽  
Vitro Fertilization ◽  
Transvaginal Oocyte Retrieval

ТРАНСВАГИНАЛЬНАЯ АСПИРАЦИЯ ФОЛЛИКУЛОВ И СПОСОБНОСТЬ OPU-ООЦИТОВ КОРОВ К ЭМБРИОНАЛЬНОМУ РАЗВИТИЮ В УСЛОВИЯХ IN VITRO

2019 ◽  
pp. 17-19
Author(s):  
G.N. SINGINA ◽  
V. HAVLICEK ◽  
N.P. TARADAYNIK ◽  
R.Y. CHINAROV ◽  
T.E. TARADAYNIK ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Blastocyst Stage ◽  
Vitro Fertilization ◽  
Maturation In Vitro ◽  
Transvaginal Aspiration ◽  
Cattle Reproduction ◽  
Ovarian Follicular Growth

Представлены результаты трансвагинальной аспирации ооцитов коров, а также оценен их потенциал к эмбриональному развитию после оплодотворения в условиях in vitro. Донорами яйцеклеток являлись половозрелые телки симментальской породы в возрасте 1619 мес. Животныедоноры (n7) перед проведением процедуры Ovum Pickup (OPU) были гормонально обработаны с целью стимуляции роста фолликулов. Количество выделенных ооцитов от индивидуальных доноров составило в среднем 7,7 ооциткумулюсных комплексов (ОКК), что соответствовало степени извлечения 54,57,7. Доля ОКК хорошего качества, рассчитанная от общего числа извлеченных ОКК, между отдельными животными существенно не различалась (значения варьировали от 60,0 до 75,0) и в среднем составила 67,21,9. ОКК с признаками нормальной морфологии подвергали in vitro процедурам созревания, оплодотворения и последующего культивирования до стадии бластоцисты. Доля раздробившихся ооцитов и выход бластоцист после in vitro осеменения яйцеклеток коров равнялась 75,7 и 24,3, соответственно. В целом от одного донора за сессию OPU было получено 1,3 эмбриона на стадии бластоцисты, содержащих в среднем 89,8 ядра. Оцененный способ экстракорпорального оплодотворения OPUооцитов коров позволяет получать эмбрионы, пригодные для замораживания и трансплантации реципиентам и может быть использован в программах по воспроизводству желаемых генотипов у крупного рогатого скота.In the present work, we report the data on transvaginal aspiration of bovine ovarian follicles and estimation of in vitro embryo development competence of collected oocytes. The oocytes were collected by ovum pickup OPU from seven 1619 monthold Simmental heifers, previously hormonallytreated in order to stimulate ovarian follicular growth. In average, 7.7 oocytecumulus complexes (OCCs) per heifer per OPU session were collected that corresponded to 54.57.7 of recovery rate. Morphologically, 60.075.0 of OCCs were the good quality and this rate did not significantly differ between the animals. Good quality OCCs (total n37) were then subjected to in vitro maturation, in vitro fertilization and in vitro embryo development up to blastocyst stage. Cleavage and blastocyst rates were 75.7 и 24.3 , respectively. In total, 1.3 blastocysts were obtained per cow per OPU session in average these blastocysts contained 89.9 cells. In conclusion, we developed the methodology of in vitro fertilization of bovine OPUcollected oocytes that allowed obtaining the blastocysts potentially suitable for freezing and transplantation to recipients. This approach can be used to multiply desired genotypes in cattle reproduction.

Effect of transuterine puncture during transvaginal oocyte retrieval on the results of human in-vitro fertilization

1989 ◽  
Vol 4 (7) ◽  
pp. 790-793 ◽  
Author(s):  
Arjoko Wisanto ◽  
Nico Bollen ◽  
Michel Camus ◽  
Esther De Grauwe ◽  
Paul Devroey ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Oocyte Retrieval ◽  
Vitro Fertilization ◽  
Human In Vitro Fertilization ◽  
Transvaginal Oocyte Retrieval

scholarly journals Response of Bovine Cumulus–Oocytes Complexes to Energy Pathway Inhibition during In Vitro Maturation

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 838
Author(s):  
Paulina Lipinska ◽  
Ewa Sell-Kubiak ◽  
Piotr Pawlak ◽  
Zofia Eliza Madeja ◽  
Ewelina Warzych
Keyword(s):  
In Vitro Maturation ◽  
Ovarian Follicle ◽  
Cumulus Cells ◽  
Selective Inhibition ◽  
Blastocyst Stage ◽  
Vitro Fertilization ◽  
Glucose Inhibition ◽  
Maturation Medium ◽  
Pathway Inhibition

Glucose or fatty acids (FAs) metabolisms may alter the ovarian follicle environment and thus determine oocyte and the nascent embryo quality. The aim of the experiment was to investigate the effect of selective inhibition of glucose (iodoacetate + DHEA) or FA (etomoxir) metabolism on in vitro maturation (IVM) of bovine COCs (cumulus–oocyte complexes) to investigate oocyte’s development, quality, and energy metabolism. After in vitro fertilization, embryos were cultured to the blastocyst stage. Lipid droplets, metabolome, and lipidome were analyzed in oocytes and cumulus cells. mRNA expression of the selected genes was measured in the cumulus cells. ATP and glutathione relative levels were measured in oocytes. Changes in FA content in the maturation medium were evaluated by mass spectrometry. Our results indicate that only glucose metabolism is substantial to the oocyte during IVM since only glucose inhibition decreased embryo culture efficiency. The most noteworthy differences in the reaction to the applied inhibition systems were observed in cumulus cells. The upregulation of ketone body metabolism in the cumulus cells of the glucose inhibition group suggest possibly failed attempts of cells to switch into lipid consumption. On the contrary, etomoxir treatment of the oocytes did not affect embryo development, probably due to undisturbed metabolism in cumulus cells. Therefore, we suggest that the energy pathways analyzed in this experiment are not interchangeable alternatives in bovine COCs.

In vitro maturation and glutathione synthesis of porcine oocytes in the presence or absence of cysteamine under different oxygen tensions: role of cumulus cells

2002 ◽  
Vol 14 (3) ◽  
pp. 125 ◽  
Author(s):  
Y. Z. Bing ◽  
Y. Hirao ◽  
K. Iga ◽  
L. M. Che ◽  
N. Takenouchi ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
In Vitro Maturation ◽  
Cumulus Cells ◽  
Vitro Fertilization ◽  
Male Pronucleus ◽  
Nuclear Maturation ◽  
Maturation Medium ◽  
Oxygen Tensions

The present study was conducted to evaluate the effect of cumulus cells on the in vitro maturation (IVM) and glutathione (GSH) synthesis of porcine oocytes cultured in the presence or absence of cysteamine under different oxygen tensions, and on their subsequent male pronucleus formation after in vitro fertilization (IVF). Cumulus-oocyte complexes (COCs) and cumulus-denuded oocytes (DOs) were cultured for 45 h in modified TCM-199 supplemented with or without 150 m cysteamine under a humidified atmosphere of 5% CO2 in air (20%�O2) or 5% CO2, 5% O2 and 90% N2. When cultured in medium supplemented with cysteamine under 20% O2 tension, the rates of COC maturation to the metaphase II (MII) stage were significantly higher than those of DOs (P<0.05). Regardless of the addition of cysteamine and oxygen tension, the rates of male pronucleus formation in COCs after IVM and IVF were significantly higher than in DOs (P<0.05). The GSH content of oocytes was significantly increased by the addition of cysteamine to the maturation medium (P<0.05), with significantly higher GSH content in COCs than in DOs (P<0.05). However, the GSH content of COCs and DOs was not significantly different when cultured in medium without cysteamine. These results indicate that cumulus cells play an important role in nuclear maturation to MII, GSH synthesis in porcine oocytes cultured in the presence of cysteamine, and subsequent male pronucleus formation after IVF.

scholarly journals Early oocyte triggering followed by in vitro maturation is a good approach in women with resistance ovary syndrome: A case-series

2021 ◽  
Author(s):  
Maryam Eftekhar ◽  
Banafsheh Mohammadi ◽  
Nasim Tabibnejad ◽  
Mohammad Hossein Razi
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
In Vitro Maturation ◽  
Case Series ◽  
Germinal Vesicle ◽  
Oocyte Retrieval ◽  
Ovary Syndrome ◽  
Vitro Fertilization ◽  
High Doses

Background: Some women represent the inability to respond to endogenous and exogenous gonadotropins during in vitro fertilization/intracytoplasmic sperm injection cycles leading to the follicular developmental arrest. The women with resistant ovaries could benefit from in vitro maturation. Case: This case-series presents pregnancies resulting from initially scheduled conventional in vitro fertilization which led to arrested cycles because of resistant ovary syndrome. The protocol was changed to early oocyte triggering for 15 women due to the small follicles ≤ 12 mm in diameter on day 15 after stimulation with high doses of exogenous gonadotrophins instead of cycle cancellation. Germinal vesicle and metaphase I oocytes that were retrieved from follicles were matured in vitro and inseminated by intracytoplasmic sperm injection. Twenty formed embryos were transferred on day 3 after oocyte retrieval. This resulted in a 30.76% chemical pregnancy out of which no abortion occurred. Therefore, we reported a 30.76% singleton ongoing pregnancy. Conclusion: It seems that early oocyte triggering followed by in vitro maturation may be considered as a good modality in women experiencing follicular resistance to gonadotropins. These cycles can be rescued from cancellation with satisfactory clinical outcomes. Key words: Arrested stimulation cycle, Early oocyte triggering, In vitro maturation, Clinical outcome.

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