166 EFFECTS OF LEPTIN AND IGF-1 ON PRE-IMPLANTATION DEVELOPMENT, DNA FRAGMENTATION, AND GENE EXPRESSION OF BOVINE EMBRYOS CULTURED IN VITRO

2006 ◽  
Vol 18 (2) ◽  
pp. 191
Author(s):  
A. Kaya ◽  
H. Sagirkaya ◽  
M. Misirlioglu ◽  
A. Gumen ◽  
E. Memili ◽  
...  
Keyword(s):  
Growth Factor ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Rt Pcr ◽  
Cell Stage ◽  
Group Iii ◽  
Fragmented Dna ◽  
Group I ◽  
Group Ii

Adequate regulatory proteins, growth factors, and hormones in in vitro embryo culture systems are important for improving the quality of embryos to a level similar to that in vivo conditions. The objective of this study was to define the effects of leptin, insulin-like growth factor-1 (IGF-1), and their combination on embryonic development, apoptosis, and expression profiles of a panel of developmentally important genes. Presumptive zygotes (16–18 h post-insemination) were randomly assigned and cultured in control (no supplementation), 5 ng/mL leptin (Group I), 100 ng/mL IGF-1 (Group II), and 5 ng/mL leptin and 100 ng/mL IGF-1 (Group III), all supplemented with 10% FCS on Day 4. On Day 8, the embryos reaching blastocyst stage were randomly either fixed for determination of DNA-fragmented nuclei by using terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) or frozen for real-time relative quantitative RT-PCR analysis. The RT-PCR was performed to assess gene transcripts of glucose transporter-1 (Glut-1), heat shock protein 70.1 (Hsp70.1), interferon tau (IF-tau), insulin-like growth factor II receptor (IGF-IIr), desmosomal glycoprotein desmocollin III (DcIII), and DNA methyltransferase 3a (Dnmt3a). A total of 349, 322, 347, and 360 zygotes were used for the control group and Groups I, II, and III, respectively. Data were analyzed with a randomized complete block design and arcsine square root transformation of the dependent variables consisting of four treatments and six replicates. Cleavage rates were 79.5, 84.2, 87.3, and 82.4% for the control group and Groups I, II, and III, respectively, and only Group II was different from the control (P < 0.05). The percentages of embryos developed beyond the 8–16 cell stage were 44.2, 48.2, 49.0, and 50.7 for the control group and Groups I, II, and III, respectively, and Group III was different from the control (P < 0.05). Percentages of blastocyst development were 26.7, 29.6, 31.5, and 29.8, and the mean blastocyst cell numbers were 96.6, 98.6, 104.4, and 104.1 for the control group and Groups I, II, and III, respectively. The percentage of nuclei with fragmented DNA were 4.2, 3.3, 2.5, and 1.9 for the control group and Groups I, II, and III, respectively. Addition of IGF-1 and/or combination with leptin (Groups II and III) decreased the number of nuclei with fragmented DNA (P < 0.01) as compared to the control group. Although the expression of Glut1, DcIII, and Igf2r did not change among the groups, IF-tau and Dnmt3a were down-regulated in Group II. Hsp70 and IF-tau were up regulated in Group III. Results indicate that addition of IGF-I in culture media improved the cleavage rate; combination with leptin also improved the development rates to 8–16-cell-stage embryos, decreased the TUNEL-positive nuclei, and altered expression of some of the developmentally important genes.

Evaluation and Comparison of Quantity and Pattern of Fluoride release from Orthodontic Adhesives: An in vitro Study

2014 ◽  
Vol 15 (1) ◽  
pp. 99-102 ◽  
Author(s):  
Devatha Ashok Babu ◽  
Sanjay Krishna Sriram ◽  
Ravindra Reddy Regalla ◽  
Chandulal Jadav ◽  
Roopa Rani S Sriram
Keyword(s):  
Orthodontic Treatment ◽  
Control Group ◽  
Base Line ◽  
Fluoride Release ◽  
Group Iii ◽  
Orthodontic Adhesives ◽  
Group I ◽  
Group Ii ◽  
Topical Fluoride

ABSTRACT Background Orthodontic treatment has gained popularity since beginning of era of dentistry. Now a day, everyone is conscious about their appearance, smile and function. During orthodontic treatment use of brackets and adhesives are common. The bonding of brackets will cause demineralization which requires the fluoridation. So the study has been undertaken to analyze the pattern of fluoride release by commercially available adhesive bonding material for the prevention of demineralization. Aim To evaluate and compare the clinical significance of quantity and pattern of fluoride release from three commercially available adhesives. Objectives To assess the pattern of fluoride release and quantity, to reduce the decalcification of enamel around orthodontic brackets and bands during treatment and to prevent further use of topical fluoride both office and self-use agents for prevention of demineralization/for remineralization. Materials and methods The comparison of quantity and pattern of fluoride release study involved commercially available bonding adhesives. They are: Group I—resin reinforced glass Ionomer light cure material (OrthoLC), Group II—fluoride releasing composite resin material (Excel) and Group III— conventional composite (Relay-a-bond) evaluated on 78 freshly extracted premolar teeth divided into three groups consisting 26 specimens in each group. The prepared specimens were stored in artificial saliva at 37°C in an incubator for subsequent fluoride analysis using ORION ion selective electrode coupled with ionalyzer 901. Fluoride analysis made at 24 hours intervals for first 3 consecutive days and thereafter at the end of 10th, 17th, 24th and 31st day of bonding. The data obtained were tabulated and interpreted by statistical analysis using ‘t’ test and one-way analysis of variance (ANOVA). Observations and Results The quantity of fluoride release in groups I and II was significant even at the end of 31st day. The one-way AVOVA showed intra and inter group significance in the quantity of fluoride release. But group III with zero fluoride release with significant decalcification on enamel which requires external use of topical fluorides. The pattern of fluoride released was 3.06 ppm for group I and 2.01 ppm for group II and was declined sharply after 24 hours; and continued to decline in subsequent weeks. Mean quantity of fluoride release by group I was 15.08 ppm were as group II was 9.02 ppm over the test period of 31 days. At the end of 31st day the group I bonding adhesive was releasing considerable amount of fluoride compared to group II whereas group III was nil. At all the periods inter and intra group mean values were highly significant. And group III acted as base line or control group as it was non fluoride releasing material. Conclusion Both the fluoride releasing adhesive bond material are useful to reduce the risk of demineralization and further prevent the usage of topical fluoride application and reduce cost and clinical visiting time for both patient and clinician. How to cite this article Regalla RR, Jadav C, Babu DA, Sriram RRS, Sriram SK, Kattimani VS. Evaluation and Comparison of Quantity and Pattern of Fluoride release from Orthodontic Adhesives: An in vitro Study. J Contemp Dent Pract 2014;15(1):99-102.

scholarly journals An in vitro comparative Evaluation of Fracture Strength of Roots Instrumentated with Self-adjusting File and Reciproc Reciprocating File, with and without Obturation

2017 ◽  
Vol 1 (1) ◽  
pp. 20-25
Author(s):  
Pooja Kabra
Keyword(s):  
Fracture Strength ◽  
Comparative Evaluation ◽  
Group Iv ◽  
Control Group ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

ABSTRACT Aim The purpose of this study was to evaluate the fracture strength of roots instrumented with the self-adjusting file (SAF; ReDent-Nova, Ra'anana, Israel) and the Reciproc reciprocating file and that were and were not obturated using the warm vertical lateral compaction technique. Materials and methods In total, 75 mandibular premolar teeth were sectioned at or below the cementoenamel junction to obtain roots 13 mm in length. The roots were balanced with respect to buccolingual and mesiodistal diameters and weight. They were distributed into four experimental groups and one control group (n = 15): No instrumentation (group I), instrumentation with SAF files but no obturation (group II), instrumentation with SAF files and obturated with warm vertical lateral compaction (group III), instrumentation with Reciproc File but no obturation (group IV), and instrumentation with Reciproc File and obturated with warm vertical lateral compaction (group V). AH Plus sealer (Dentsply DeTrey, Konstanz, Germany) was used along with gutta-percha points. One week later, a vertical load was applied to the specimen's canal until fracture occurred. Data were statistically analyzed using one-way analysis of variance (p = 0.05). Results The mean fracture load was 312.83 N for group I, 297.35 N for group II, 359.15 N for group III, 231.51 N for group IV, and 275.81 N for group V. Conclusion The fracture resistances exhibited a statistically significant difference between all the groups. Teeth instrumented by SAF exhibited a better fracture resistance. How to cite this article Tyagi S, Choudhary E, Kabra P, Chauhan R. An in vitro comparative Evaluation of Fracture Strength of Roots Instrumentated with Self-adjusting File and Reciproc Reciprocating File, with and without Obturation. Int J Clin Dent Res 2017;1(1):20-25.

scholarly journals An In Vitro Comparison of the Apical Seal Produced by Three Different Thermoplasticized Guttapercha Obturation Techniques in Oval Canals

2015 ◽  
Vol 03 (01) ◽  
pp. 039-046
Author(s):  
Geetanjali Bansal ◽  
Ajay Bansal ◽  
Bhupinder Padda
Keyword(s):  
Control Group ◽  
Group Iii ◽  
Dye Penetration ◽  
Sealing Ability ◽  
India Ink ◽  
Nail Polish ◽  
Group I ◽  
Group Ii ◽  
The Difference

AbstractThe aim of this study was to evaluate and compare the sealing ability to obturate oval canals with three thermoplasticizedguttaperchaobturation techniques taking lateral condensation technique as the control. Ninety-five freshly extracted teeth were decoronated at 2mm coronal to CEJ. Biomechanical preparation was done using step back technique. The teeth were divided into three experimental groups of 30 teeth each and one control group of 5 teeth. The group I (control group) was obturated with lateral condensation technique, group II obturated with injectable thermoplasticizedguttapercha technique, group III obturated with thermoplasticizedguttapercha with downpack and backfill technique andgroup IV obturated with core carrier thermoplasticizedguttapercha technique. The sealability of each technique was assessed by a dye penetration method. The roots were given two full layers of nail polish varnish except apical 2mm. Specimens were then immersed in India ink for 48 hours. Robertson’s technique was used to clear the specimens. The linear dye penetration was measured from anatomic apex to the deepest extent of dye penetration in a coronal direction using triocular stereomicroscope at 10 × magnification. The mean dye leakage of group I was 2.6700mm;group II 0.1713mm;group III 3.3977mm; group IV 2.3210mm. When the means of all the four groups were compared using Kruskal Wallis test the difference was found to be very highly significant with the value<.001**, meaning there by that group II is significantly better than the other three groups as far as sealing ability is concerned.

scholarly journals 160 IN VIVO-CULTURE OF BOVINE EMBRYOS: TRANSFER OF SEMEN PRE-INCUBATED OOCYTES, ZYGOTES AND 4 TO 8 CELL STAGE EMBRYOS INTO THE BOVINE OVIDUCT

2005 ◽  
Vol 17 (2) ◽  
pp. 231
Author(s):  
V. Havlicek ◽  
F. Wetscher ◽  
T. Huber ◽  
M. Gilles ◽  
D. Tesfaye ◽  
...  
Keyword(s):  
Embryo Development ◽  
Calf Serum ◽  
Bovine Embryos ◽  
Cell Stage ◽  
Group Iii ◽  
Group I ◽  
In Vivo Culture ◽  
Group Ii

Oviduct as well as oocyte and embryo development are subject to developmental changes which have crucial effects on the application of in vivo culture. The present study aimed at optimizing in vivo culture of IVP bovine embryos at different developmental stages in the bovine oviduct. Cumulus oocyte complexes (COC) were collected from slaughterhouse ovaries, matured in vitro for 22 h and assigned to four groups. In groups I and II, oocytes were pre-incubated for 3 to 4 h with 5 × 106 sperm/mL, and then immediately transferred to recipients, which had just completed ovulation (group I), or kept in vitro for a further 12 to 18 h and transferred to Day 1 synchronized recipients (group II). In groups III and IV, COC were subjected to standard IVF/IVC; then embryos were either transferred at the 4- to 8-cell stage on Day 3 into the oviducts of Day 3-synchronized recipients (group III) or kept in vitro for a further 4 to 5 days (group IV). Thirty-four 18- to 30-month-old temporary recipients were synchronized using a standard Ovsynch protocol. COC and embryos were transferred and re-collected by transvaginal endoscopy. COC or embryos were loaded into a 180° curved glass capillary, which was inserted via the infundibulum 5 to 8 cm deep into the ampulla ipsilateral to the CL. On recipient Day 7, a 90° curved metal canula served for tubal flushing prior to conventional uterine embryo flushing. Sixty mL of PBS containing 1% fetal calf serum were rinsed through the oviduct into the uterus and a further 400 mL of medium were finally used for flushing of the uterine horn and collected via an embryo filter. Embryo development was evaluated directly after flushing (Day 7) and on Day 8. For statistical analysis (ANOVA), the blastocyst rates (Days 7 and 8) in group III were related to COC corrected by the collection rate. In group I, 575 COC were transferred to 11 recipients and 420 (73%) were re-collected as oocytes or embryos. The blastocyst yields on Day 7 and Day 8 were 23% (97) and 25% (104), respectively. In group II, the transfer of 489 presumptive zygotes into 13 heifers resulted in only 175 re-collected (36%), of which 15% developed into blastocysts (Day 7: 26; Day 8: 27). Ten heifers (group III) served for in vivo culture of 643 embryos at the 4- to 8-cell stage. On Day 7, 568 (88%) embryos were flushed and 171 (30%) reached the blastocyst stage. A further 24 h culture in vitro finally resulted in 244 (42%) blastocysts. The complete in vitro production system delivered 13% (63/477) blastocysts on Day 7 and 34% (161/477) blastocysts on Day 8. The collection rates (P < 0.001) and the blastocyst rates on Day 7 (P < 0.05) and Day 8 (P < 0.001) differed significantly in all groups. The present data demonstrate that the developmental stage of transferred complexes has an influence on embryo recovery as well as an embryo development. This work was supported by Austrian BMBWK and BMLFUW (#1227).

114 Insulin-Like Growth Factor (IGF)2 and IGF2 Receptor (IGF2R) Murine Blastocyst Transcriptional Response after High Gaseous Pressure Exposure at 8-Cell Stage

2018 ◽  
Vol 30 (1) ◽  
pp. 196
Author(s):  
B. S. Becker ◽  
F. J. F. Collares ◽  
A. V. Gonsiorosky ◽  
C. Rodrigues de Freitas ◽  
D. A. Mentz ◽  
...  
Keyword(s):  
Growth Factor ◽  
Internal Control ◽  
Growth And Development ◽  
Paternal Allele ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Mus Musculus Domesticus ◽  
Cell Stage ◽  
Imprinted Gene

Insulin-like growth factor 2 (IGF2) is a pleiotropic hormone encoded by an imprinted gene expressed in the paternal allele of mammals, and acts in physiological responses including cell proliferation, differentiation, and development. It is mediated through the IGF1R signalling pathway, whereas the IGF2R, a maternally imprinted gene, acts on lysosomal IGF2 degradation. As imprinted genes, both Igf2 and Igf2r expressions are more susceptible to dysregulation by environmental factors. This study aimed to evaluate the effect of exposure of 8-cell-stage murine embryos to 16 MPa of high gaseous pressure (HGP) on the relative Igf2 and Igf2r mRNA abundance in resulting blastocysts following in vitro culture (IVC). Day-3 embryos were recovered from superovulated Mus musculus domesticus females. Eight-cell embryos were exposed to 16 MPa HGP for either 2 h (P1 group) or 4 h (P2 group), with a Control group not exposed to HGP. Immediately after recovery or HGP exposure, embryos were in vitro-cultured for 48 h in mKSOM medium supplemented with 0.4% BSA at 37.5°C, 5% CO2, 5% O2, 90% N2, and saturated humidity. Resulting blastocysts were collected in pools of 10 and stored at –80°C, pending analysis. Following total mRNA extraction, cDNA syntesis and RT-qPCR were performed according to manufacturers. Values were normalized to the internal control Ppia gene. Relative gene expression was calculated using the 2−ΔΔ Ct approach. Blastocyst rates after IVC were compared by the Chi-squared test (P < 0.05), with relative Igf2 and Igf2r expression data and Igf:Igf2r ratio analysed by ANOVA, after log-transformation when needed, with pairwise comparisons done by the Tukey test (P < 0.05). No differences in blastocyst rates after IVC were observed among groups (Control: 94.2%; P1: 95.4%; P2: 94.1%). However, the Igf2 mRNA relative abundances in blastocysts were 6.3- and 4.2-fold lower in P1 (P < 0.01) and P2 (P = 0.07) than in the Control group, respectively. Likewise, the Igf2r relative transcription levels were 6.6- and 2.2-fold down-regulated in blastocysts from the P1 (P < 0.001) and P2 (P < 0.01) groups, respectively, when compared with controls. Although the relative expression for both genes followed a down-regulation pattern in blastocysts exposed to HGP at the 8-cell stage, the Igf2:Igf2r ratio was 1.9-fold lower in blastocysts in the P2 group (P < 0.05) than Controls, which was similar to the P1 group, indicating a potential stress adaptation response for embryo growth and development after exposure to HGP in the P1 group. It is known that cells under certain conditions of stress may halter growth and development as a response to initiate cellular events to maintain viability. Results from this study appear to translate such response process to HGP in both experimental groups. However, as embryo development and the Igf2:Igf2r ratio in embryos were similar between the Control and the P1 group, exposure to 16 MPa HGP for 2 h at the 8-cell-stage embryo does not seem to affect cell signalling to growth and proliferation up to the blastocyst stage.

scholarly journals The Effectiveness of Salavadora Persica (Siwak) Petroleum Ether Extract As An Intracanal Medicament Used in Endodontic Therapy: An In Vitro Study

2021 ◽  
Author(s):  
Nahla Ayoub ◽  
Nadia Badr ◽  
Saeed Alghamdi ◽  
Arwa Alzahrani ◽  
Rahaf Alsulimani ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Control Group ◽  
Salvadora Persica ◽  
Group Iii ◽  
Group I ◽  
Freeze Dried ◽  
Oil Extract ◽  
Group Ii

Abstract Background: Salvadora persica L. (Siwak) of the family Salvadoraceae has been used for many centuries as oral hygiene tools, particularly in Saudi Arabia. The current study aimed at developing a new root canal medicament based on the extract of Salvadora persica (SP). The antibacterial activity against Enterococcus faecalis was determined compared with the gold standard intracanal medicament, Ca (OH)2. Materials and Methods: Freeze-dried Siwak sticks were extracted with Petroleum ether to yield an oily extract (SPE). GC-MS analysis was carried out to identify SPE components, whereas 32 compounds were identified (89.09%), with main components of benzyl isothiocyanate (BITC) (33.32%) and steroids (34%). Firstly, the consistency of the experimental medicament was accomplished according to ANSI/ADA Specification no 57. SPE assessment as intracanal medicament was conducted through an in vitro assay on 45 single-rooted mandibular premolars inoculated of 5 μL of E. faecalis. Colony-forming units (CFU) were counted before the application of experimental intracanal medicament (CFU-1) and then after seven days of its application (CFU-2). Group I: Siwak extract-based intracanal medicament, Group II positive control group received Ca(OH)2 paste, and Group III: negative control group received no medication. Results: CFU before and after using Siwak based medicament recorded statistically significant reduction (P=0.006) as well as those of Group II when Ca(OH)2 was applied (P=0.01). On comparing both medicaments with Group III, there were significant differences; (P=0.00) and (P=0.007), respectively. Siwak oil extract-based medication proved complete eradication of E. faecalis.Conclusion: SPE has a good potential as an intracanal medicament, besides the cleaning and shaping during endodontic therapy. This finding could be referred to as the high content of BITC bactericidal against pulpal and periodontal pathogens, in synergism with other antimicrobial components, representing 70.71% of SP oil extract.

Features of the cervix uteri in women of reproductive age with endometrial polyps and micropolyps

2016 ◽  
pp. 108-111
Author(s):  
T.F. Tatarchuk ◽  
◽  
D.G. German ◽  
Keyword(s):  
Reproductive Age ◽  
Cervix Uteri ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Endometrial Polyps ◽  
Group Iii ◽  
Aggressive Form ◽  
Group I ◽  
Group Ii ◽  
Control Group Group

The article presents the comparative analysis of the state of the cervix in women with endometrial polyps and micropolyps. Patients and methods. The study involved 130 patients aged 18-35 years: 70 patients with endometrial polyps (group I), 30 patients with micropolyps (group II) and 30 patients of the control group (group III). Results. According to the anamnesis of women in the I group were significantly more frequent diseases of the cervix, which corrected physical surgery methods, in particular cryodestruction. In group II, the representatives of these indicators were similar to healthy. Normal colposcopic picture met significantly less frequently in patients and I, and II group. The differences in the incidence of HPV high oncogenic risk in all groups were not statistically significant. Conclusion. Destructive methods used in the detection of any changes in the cervix are often overly aggressive, form scars and contributing to inflamaciones process. In the chain of events leading to the formation of PE, cervical pathology and its correction can take the basic place. Key words: endometrial polyp, micropolyps, chronic endometritis, uterine cervix, colposcopy.

scholarly journals Irisin levels in relation to metabolic and liver functions in Egyptian patients with metabolic syndrome

2016 ◽  
Vol 94 (4) ◽  
pp. 359-362 ◽  
Author(s):  
Fatma H. Rizk ◽  
Samah A. Elshweikh ◽  
Amira Y. Abd El-Naby
Keyword(s):  
Metabolic Syndrome ◽  
Liver Enzymes ◽  
Resistance Index ◽  
Control Group ◽  
Model Assessment ◽  
Group Iii ◽  
Group I ◽  
Elevated Liver Enzymes ◽  
Liver Functions ◽  
Group Ii

Irisin is a new myokine that is suspected to influence metabolic syndrome (MetS). However, there is a great controversy with respect to its level in cases of MetS and its correlation with different metabolic parameters. The present study assesses irisin levels in MetS patients and studies its relationship to metabolic and liver functions to evaluate the possible role of the liver in regulation of this level. Sixty subjects were included in this experiment, who were divided into 3 groups: group I (normal control), group II (MetS patients with normal liver enzymes), and group III (MetS with elevated liver enzymes and fatty liver disease). Serum irisin levels showed significant increases in groups II and III compared with group I, and significant increases in group III compared with group II. Also, irisin levels were positively correlated with body mass index, serum triglycerides, homeostatic model assessment of insulin resistance index (HOMA-IR), and liver enzymes. We concluded that serum irisin levels increased in patients with MetS, especially those with elevated liver enzymes, and had a positive correlation with parameters of lipid metabolism and glucose homeostasis with the possibility of hepatic clearance to irisin.

scholarly journals Assessment of shear bond strength of brackets bonded by direct and indirect techniques: an in vitro study

2012 ◽  
Vol 17 (4) ◽  
pp. 1-7 ◽  
Author(s):  
Roberto Hideo Shimizu ◽  
Karlos Giovani Grando ◽  
Isabela Almeida Shimizu ◽  
Augusto Ricardo Andriguetto ◽  
Ana Cláudia Moreira Melo ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Adhesive System ◽  
Vitro Study ◽  
Group Iii ◽  
Indirect Bonding ◽  
Group I ◽  
Group Ii

OBJECTIVE: This in vitro study was designed to evaluate the shear bond strength (SBS) of orthodontic metal brackets bonded by direct and indirect techniques. METHODS: Thirty healthy human maxillary premolar teeth were used. The teeth were divided into three groups of 10 teeth each: Group I - indirect bonding with SondhiTM Rapid-Set system (3M/Unitek), Group II - indirect bonding with TransbondTM XT adhesive system (3M/Unitek) and Group III - direct bonding with TransbondTM XT adhesive system (3M/Unitek). After bonding and obtaining the specimens for the study, the specimens were subjected to SBS testing in a universal testing machine (Emic, model DL-500). The Kolmogorov-Smirnov test was applied to ascertain that the data had a normal distribution and the Bartlett test to check whether there was homogeneity of variance. One-factor analysis of variance was performed and, subsequently, Tukey's test for paired means. A 5% significance level was adopted. RESULTS: The results of Group I were 67.6 (N) and 5.9 (MPa); Group II, 68.9 (N) and 6.1 (MPa) and Group III (control), 92.5 (N) and 8.1 (MPa). CONCLUSION: It can therefore be concluded that the means for Group III were significantly higher compared with Groups I and II in both Newton (N) and Megapascal (MPa) values. The means attained by the indirect bonding technique used in Groups I and II, however, exhibited no statistically significant differences.

Suppression of Mesangial-Cell Proliferation by Trapidil in Glomerulonephritis Induced by Anti-Thymocyte Serum in Rats

1995 ◽  
Vol 23 (6) ◽  
pp. 458-466 ◽  
Author(s):  
M S Razzaque ◽  
M Cheng ◽  
T Taguchi
Keyword(s):  
Cell Proliferation ◽  
Body Weight ◽  
Single Dose ◽  
Growth Factor ◽  
Mesangial Cell ◽  
Platelet Derived Growth Factor ◽  
Group Iii ◽  
Group I ◽  
Mesangial Cell Proliferation ◽  
Group Ii

Trapadil (Mochida Pharmaceuticals, Japan), an antiplatelet drug, suppresses the growth of several cell types and is thought to antagonize platelet-derived growth factor. The effects of trapidil on mesangial-cell proliferation in glomerulonephritis induced by anti-thymocyte serum in Wistar rats were investigated. Control rats were treated with phosphate-buffered saline (group I); group II rats were injected with a single dose of anti-thymocyte serum (8 ml/kg body weight), and group III rats were treated with both a single dose of anti-thymocyte serum (8 ml/kg body weight) and with trapidil (5 mg/kg body weight/day). Three rats in each group were killed on day 3, and the other three on day 10. Control rats showed no significant histological changes on day 3 or day 10. In group II, on day 3, there was a marked decrease in glomerular cell numbers, with mesangiolysis. Histologically severe mesangial-cell proliferation with expansion of mesangial areas was noted on day 10. None of the rats in group III showed mesangial alterations, histologically, indicating that mesangial-cell proliferation was suppressed by trapidil. This suppression may result from antagonism of the binding of platelet derived growth factor to the specific surface receptors in the mesangial cells. Trapidil may have clinical value in the treatment of mesangial-cell proliferative glomerular diseases.

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