scholarly journals 94 COMPARING DIFFERENT LEVELS OF OSMOLARITY AND pH OF LACTOSE EXTENDER ON THE VIABILITY OF SPERMATOZOA IN THE BACTRIAN CAMEL (CAMELUS BACTRIANUS)

2005 ◽  
Vol 17 (2) ◽  
pp. 197 ◽  
Author(s):  
S. Mosaferi ◽  
A. Niasari-Naslaji ◽  
A.A. Gharahdaghi ◽  
A. Abarghani ◽  
A. Ghanbari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Bactrian Camel ◽  
Mechanical Removal ◽  
Semen Collection ◽  
Arcsine Transformation ◽  
History Of ◽  
Semen Extender ◽  
Different Levels ◽  
Artificial Vagina

Lactose has been used widely as a semen extender for camels although in the absence of evidence illustrating its suitablility. Considering the osmolarity (316.1 ± 1.48 mOsm/kg) and pH (7.4 ± 0.03) of Bactrian camel semen (Mosaferi S et al. 2004 Theriogenology, in press), the objective of this study was to investigate the effect of osmolarity and pH of lactose extender on the viability of Bactrian camel spermatozoa. In Experiment I, with pH adjusted to 6.9, the effect of lactose concentrations of 9, 10, 11, 12, and 13% with an osmolarity of 290, 333, 350, 376, and 419 mOsm/kg, respectively, on the viability of spermatozoa was investigated. In Experiment II, with lactose fixed at 10%, the effect of extender with pH of 5.9, 6.9, 7.5, 7.9, and 8.9 on the viability of spermatozoa was examined. All extenders contained 20% egg yolk. In both experiments, semen was collected from camels with a sound history of semen quality and fertility (n = 3), using a modified artificial vagina, and divided into different treatments after mechanical removal of semen viscosity (Mosaferi et al. 2004). Progressive forward motility of spermatozoa was examined at the time of semen collection and at 4, 12, and 24 h after incubation at 4°C. Data were analyzed using the GLM procedure in SAS/STAT (SAS Institute, Inc., Cary, NC, USA) after arcsine transformation. At the time of semen dilution, the progressive forward motility of spermatozoa was greater at osmolarity of 290 (28.5%), 333 (34%), and 350 (31%) compared to 376 (13.5%) and 419 (1%) mOsm/kg (P < 0.05). The same trend in the progressive forward motility of spermatozoa was noticed after 4 h of incubation at 4°C; although a significant decrease (P < 0.05) occurred at 290 (11%), 333 (18 %) and 350 (16%). After 12 and 24 h of incubation at 4°C, the progressive forward motility of spermatozoa was less than 10% at 333 and 350 mOsm/kg (P < 0.05). At the time of semen dilution, the progressive forward motility of spermatozoa was greater (P < 0.05) at pH 6.9 (35.5%) and 7.5 (18%) compared to pH 5.9 (0%), 7.9 (7.5%) and 8.9 (2.5%). The same trend in the progressive forward motility of spermatozoa was observed after 4 h incubation at 4°C; although, a significant decrease (P < 0.05) occurred at pH 6.9 (15%) and 7.5 (9%) at this time. After 12 h incubation at 4°C, the progressive forward motility of spermatozoa was less than 5% at pH 6.9 and 7.5 (P < 0.05). In conclusion, 10% (333 mOsm/kg) and 11% (350 mOsm/kg) lactose, at the adjusted pH of 6.9, were the most suitable concentrations of lactose extender for preserving Bactrian camel semen for less than 4 h after which the viability of spermatozoa deteriorated significantly in this extender. The authors wish to thank the director and station staff of Bactrian Camel Research Center at Jahadabad, Meshkinshahr, Ardabil, for kind provision of facilities and assistance throughout the experiment.

104 COMPARING DIFFERENT LEVELS OF OSMOLALITY OF SUCROSE EXTENDER ON THE VIABILITY OF SPERMATOZOA IN BACTRIAN CAMEL (CAMELUS BACTRIANUS)

2006 ◽  
Vol 18 (2) ◽  
pp. 160 ◽  
Author(s):  
S. Mosaferi ◽  
A. Niasari-Naslaji ◽  
N. Bahmani ◽  
A. A. Gharahdaghi ◽  
A. Abarghani ◽  
...  
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Bactrian Camel ◽  
Dromedary Camel ◽  
Camelus Bactrianus ◽  
Semen Collection ◽  
History Of ◽  
Different Levels ◽  
Artificial Vagina

Disaccharides have been used as an extender for dromedary camel semen (Bravo et al. 2000 Anim. Reprod. Sci. 62, 173-193). More recently we have investigated the effect of different concentrations of lactose extender on the viability of Bactrian camel spermatozoa (Mosafer et al. 2005 Reprod. Fertil. Dev. 17, 197). Considering the osmolality (316.1 � 1.48 mOsm/kg) and pH (7.4 � 0.03) of Bactrian camel semen (Mosaferi et al. 2005 Theriogenology 63, 92-101), the objective of this study was to investigate the effect of osmolality of sucrose extender on the viability of Bactrian camel spermatozoa. Sucrose at the concentrations of 9, 10, 11, 12, and 13% with osmolalities of 292, 331, 356, 386, and 410 mOsm/kg, respectively, were prepared. All extenders contained 20% egg yolk and antibiotics, with pH adjusted to 6.9. Semen was collected from camels with a sound history of semen quality and fertility (n = 3) using a modified artificial vagina and divided into different treatments after mechanical reduction of semen viscosity (3). Progressive forward motility of spermatozoa was examined at the time of semen collection and at 4, 12, and 24 h after incubation at 4�C. Data were analyzed using the GLM procedure in SAS/STAT after arcsin transformation. At the time of semen dilution, the progressive forward motility of spermatozoa was greater at osmolality of 331 (23%) compared with 292 (1%), 386 (6%), and 410 (3.5%) mOsm/kg (P < 0.05). No progressive forward motility of spermatozoa was noticed after 4 h incubation at 4�C at osmolalities of 292, 386, and 410 mOsm/kg. At this time, a significant decrease (P < 0.05) of progressive forward motility occurred at osmolalities of 331 (4%) and 356 (0.5%) compared with that of the time of dilution. After 12 and 24 h incubation at 4�C, no progressive forward motility of spermatozoa was detected in any of these extenders. In conclusion, 10% sucrose (331 mOsm/kg) at the adjusted pH of 6.9 was the most suitable concentration of this disaccharide for preserving Bactrian camel semen for less than 4 h under chilled conditions.

scholarly journals 96 A NOVEL EXTENDER FOR PRESERVATION OF BACTRIAN CAMEL (CAMELUS BACTRIANUS) SEMEN

2005 ◽  
Vol 17 (2) ◽  
pp. 198 ◽  
Author(s):  
A. Niasari-Naslaji ◽  
S. Mosaferi ◽  
A.A. Gharahdaghi ◽  
A. Abarghani ◽  
A. Ghanbari ◽  
...  
Keyword(s):  
Semen Quality ◽  
Egg Yolk ◽  
Bactrian Camel ◽  
Dromedary Camel ◽  
Persian Language ◽  
Semen Collection ◽  
Arcsine Transformation ◽  
History Of ◽  
Collection Time ◽  
Better Than

Lactose and Green buffer (IMV, France) are the most commonly used extenders for camel semen. The viability of Bactrian camel spermatozoa in lactose extender is reduced after 4 h of incubation at 4°C (unpublished data). Although Green buffer is used for dromedary camel semen, there are no data indicating its effectiveness for Bactrian camel semen. More recently, we reported that the osmolality and pH of Bactrian camel semen are 316.1 ± 1.48 mOsm/kg and 7.4 ± 0.03, respectively. The objective of this study was to compare three different semen extenders, to determine if a TRIS-based diluent (SHOTOR* Diluent), a completely defined diluent, can maintain cooled camel sperm as effectively as established diluents. SHOTOR Diluent consists of 2.6 g TRIS, 1.35 g citric acid, 1.2 g glucose, and 0.9 g fructose in 100 mL of deionized water, with an osmolality of 330 mOsm/kg and pH of 6.9. SHOTOR Diluent, lactose, 10% (w/v), with an osmolality of 330 mOsm/kg and pH of 6.9, and Green buffer were compared in this study. All extenders contained 20% egg yolk. Semen was collected from bulls with a sound history of semen quality and fertility (n = 3), using a modified artificial vagina, and divided equally into the different extenders (Mosaferi S et al. 2004 15th Int. Cong. Anim. Reprod. 2, 520; Mosaferi S et al. 2004 Theriogenology, in press). Progressive forward motility and percentage of live spermatozoa were examined at the time of semen collection (time 0) and after 4, 12, and 24 h of incubation at 4°C. Data were analyzed using the GLM procedure in SAS/STAT after arcsine transformation. The forward progressive motilities of spermatozoa at 0, 4, 12, and 24 h after semen collection were 65.5, 54, 44.5, and 36.5% in SHOTOR Diluent; 31, 18.5, 8.5, and 0% in 10% lactose; and 60.5, 54.5, 33, and 32.5 % in Green buffer, respectively (Table 1). The percentage of live spermatozoa at 0, 4, 12, and 24 h were 84.5, 84, 81 and 74.5% in SHOTOR Diluent; 80, 79.5, 72.5, and 56.5% in 10% lactose; 89, 82.5, 82.5, and 77.5% in Green buffer, respectively (P > 0.05). The progressive forward motility of spermatozoa did not significantly decrease by 12 h at 4°C in SHOTOR Diluent (P > 0.05; Table 1), whereas it significantly decreased after 4 h and 12 h of incubation at 4°C in Green buffer and 10% lactose, respectively (P < 0.05; Table 1). Further decrease in the progressive forward motility occurred in all extenders after 24 h at 4°C (P < 0.05; Table 1). In conclusion, SHOTOR Diluent is better than Green buffer and 10% lactose as an extender for chilled storage of Bactrian camel semen for 12 hat4°C. Table 1. The progressive forward motility of Bactrian camel spermatozoa extended in SHOTOR Diluent (1), 10% lactose (2) and Green buffer (3) at the time of semen collection (time 0) and after 4, 12, and 24 h of incubation at 4°C *Shotor means camel in the Persian language. The authors wish to thank the director and station staff of Bactrian Camel Reseach Center at Meshkinshahr, Ardabil, for providing facilities and kind assistance throughout the experiment.

105 COMPARISON BETWEEN TWO EXTENDERS FOR CRYOPRESERVATION OF BACTRIAN CAMEL SEMEN

2006 ◽  
Vol 18 (2) ◽  
pp. 161
Author(s):  
A. Niasari-Naslaji ◽  
S. Mosaferi ◽  
A. A. Gharahdaghi ◽  
A. Abarghani ◽  
A. Ghanbari ◽  
...  
Keyword(s):  
Citric Acid ◽  
Liquid Nitrogen ◽  
Egg Yolk ◽  
Deionized Water ◽  
Bactrian Camel ◽  
Equilibration Time ◽  
Persian Language ◽  
Significant Difference ◽  
Arcsine Transformation ◽  
Artificial Vagina

A Tris-based extender (SHOTOR diluent) has been developed for preserving Bactrian camel semen at 4�C (Niasari-Naslaji et al. 2005 Reprod. Fertil. Dev. 17, 198 (abstr.)). The present study investigated the possibility of utilizing the SHOTOR diluent for the cryopreservation of Bactrian camel semen. A modified bovine artificial vagina (Masaferi et al. 2005 Theriogeology 63, 92-101) was used to collect semen from three fertile bulls. The viscosity of the semen was reduced mechanically (Mosateri et al. 2005) and the homogenized semen was divided equally into two parts. Each part was sequentially diluted with either IMV buffers (Green buffer: first extender; White buffer: second extender; IMV, France) or SHOTOR diluents (without glycerol: first extender; with 12% glycerol: second extender). SHOTOR diluent consists of 2.6 g TIS, 1.35 g citric acid, 1.2 g glucose, and 0.9 g fructose in 100 mL of deionized water, with an osmolality of 330 mOsm/kg and pH of 6.9. All extenders had 20% egg yolk and antibiotics. The semen was diluted at the ratio of 1:1 with the first extender. The diluted semen was then cooled within 2 h to 4�C. At this temperature, the second extender was added at the same volume as the diluted semen in three steps with an equal volume, 10 min apart. After a 30-min equilibration time, beginning after addition of the last fraction of the second extender, the diluted semen was loaded into 0.5-mL straws at a concentration of 50 � 106 sperm per straw. The straws were maintained for 20 min at 4 cm above the liquid nitrogen surface, after which they were plunged into liquid nitrogen. The semen was thawed at 40�C water bath for 20 s. Progressive forward motility of spermatozoa was assessed at the time of dilution and immediately after thawing of the semen. The experiment was replicated four times. Data were analyzed using GLM procedure in SAS/STAT after arcsine transformation. At the time of dilution, there was no significant difference in progressive forward motility of spermatozoa between IMV buffers (51.8%) and SHOTOR diluent (61%; P > 0.05). However, after thawing, there was a significant decrease in progressive forward motility of spermatozoa in IMV buffers (4.2%) compared to SHOTOR diluent (29.9%, P < 0.05). In conclusion, in this experiment, SHOTOR diluent was more efficient for cryopreserving Bactrian camel semen than IMV extender. Shotor means camel in the Persian language.

Effect of different levels of egg-yolk on freezability of Jakhrana buck semen

2016 ◽  
Author(s):  
Narendra Kumar ◽  
B. Rai ◽  
Chetna Gangwar ◽  
S. A. Lone ◽  
Anshuman Kumar ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Egg Yolk ◽  
Osmotic Swelling ◽  
Semen Collection ◽  
Sperm Abnormality ◽  
Before And After ◽  
Different Levels ◽  
Artificial Vagina ◽  
Intensive System ◽  
Acrosomal Integrity

The present study was designed to determine the effect of different levels of egg-yolk on freezability of Jakhrana buck semen. Six healthy Jakhrana bucks (BW=30 ± 2kg, age=12 ± 0.5 month) were used for semen collection. These bucks were maintained under semi-intensive system at Jakhrana Unit of C.I.R.G. Makhdoom, Mathura. A total of 48 ejaculates (6 bucks × 8 replicates) were collected twice a week using artificial vagina. Each ejaculate was divided into 4 groups (G1, G2, G3 and G4). The G1, G2, G3 and G4 were extended with Tris-egg yolk-citric acid- fructose-glycerol (TEYCFG) extenders containing 5, 10, 15 and 20% egg yolk level, respectively. Each ejaculate was evaluated for sperm motility, viability, abnormality, and hypo-osmotic swelling (HOS) response and acrosome integrity before and after freezing. At pre-freeze stage no significant (P>0.05) difference in sperm motility and viability was found among all groups. Sperm abnormality was significantly (P<0.05) higher in G4 as compared to other groups (G1, G2, G3). The HOS response and acrosomal integrity was significantly (P<0.05) higher in G1, G2 and G3 as compared to G4. However, no significant (P>0.05) difference was observed in HOS response and acrosomal integrity among G1, G2 and G3. At post thaw stage, sperm motility, viability and HOS response was significantly (P<0.05) higher in G1 and G2 as compared to G3 and G4. Sperm abnormality was significantly (P<0.05) lower in G2 as compared to other groups. The acrosomal integrity was significantly (P<0.01) higher in G1 and G2 as compared to G3 and G4. It is concluded that 10% egg yolk in Tris based extender may be the best for successful cryopreservation of Jakhrana buck semen.

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals Changes in Quality of Native and Frozenthawed Semen in Relation to Two Collections Performed in a 24-hour Interval and Adition of Clarified Egg Yolk to Extender

2016 ◽  
Vol 47 (2) ◽  
pp. 60-67
Author(s):  
P. Folková ◽  
J. Šichtař ◽  
O. Šimoník ◽  
A. Dokoupilová ◽  
R. Rajmon
Keyword(s):  
Sperm Motility ◽  
Egg Yolk ◽  
Semen Collection ◽  
Semen Extender

Abstract The aim of the study was to evaluate the effect of repeated semen collection and the substitution of normal egg yolk with clarified egg yolk to commercially produced semen extender on qualitative parameters of frozen-thawed canine semen. Two semen collections were scheduled in a 24-hour interval and in each of six dogs, three 1st and three 2nd collections were performed. The frozen-thawed sperm samples were prepared either with clarified or normal egg yolk and motility and viability were evaluated. The effect of the sequence of semen collection was demonstrated by significant differences in motility and also in viability of sperms both in native and frozen-thawed ejaculate. The percentage of viable sperms was significantly higher in samples from the 2nd compared to the 1st collection. This trend was the same also in motility except in native ejaculate. The addition of clarified egg yolk was beneficial for higher survival of sperms immediately after thawing and also after 30 min of incubation, compared to samples with normal egg yolk. Sperm motility evaluated after thawing was higher in samples with clarified egg yolk, without an apparent connection with semen collection sequence. The decrease of values of the qualitative parameters of sperms observed in the period of 30 min of incubation was significantly slowed down when clarified egg yolk was used. This was especially obvious in samples from the 2nd collection.

Comparative ejaculatory response, fresh and frozen semen quality and fertility to artificial vagina vs electroejaculation method of semen collection in mithun ( Bos frontalis ) bulls

Andrologia ◽  
2021 ◽  
Author(s):  
Saddamhusen Mabusab Nadaf ◽  
Vikram Ramesh ◽  
Moonmoon Mech ◽  
Meraj Haider Khan ◽  
Fazal Ali Ahmed ◽  
...  
Keyword(s):  
Semen Quality ◽  
Semen Collection ◽  
Frozen Semen ◽  
Artificial Vagina

scholarly journals Effect of sunflower lecithin on Kalahari Red goat semen during cryopreservation

2018 ◽  
Vol 51 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Sakirat Opeyemi Adeyanju ◽  
James Olatinbo Daramola ◽  
Jimoh Alao Olanite ◽  
Olufiropo Samson Awokola
Keyword(s):  
Soybean Lecithin ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Domestic Animal ◽  
Arginase Activity ◽  
Semen Parameters ◽  
Phospholipid Content ◽  
Acrosome Integrity ◽  
Semen Extender ◽  
Different Levels

Abstract Soybean lecithin had been used as an alternative to egg yolk in domestic animal semen extender during cryopreservation due to its characteristic phospholipid content which played a major cryoprotective role. This composition of soybean lecithin informed the replacement of soybean with sunflower lecithin (SL) in the extender for the Kalahari Red (KR) buck semen cryopreservation in this study. Effect of different levels of SL on the quality of the KR buck semen during cryopreservation using slow freezing method was evaluated. Semen samples were collected from four KR bucks of between two and two and half of age using artificial vagina, evaluated for motility and then diluted in extenders containing different levels of SL (1.5%, 3.0% and 4.5%) as experimental group and 0% SL or 20% egg yolk as control. Semen parameters including motility, acrosome integrity (AcI), membrane integrity (MI), malondialdehyde (MDA) concentration, cholesterol level and seminal arginase activity were evaluated for. The results showed that motility, acrosome integrity (AI) and membrane integrity were comparable at 0%, (22.00 ± 4.58, 82.00 ± 3.51 and 96.00 ± 2.03); 1.5%, (23.00 ± 2.08, 87.00 ± 3.79 and 89.00 ± 2.08); 3.0%, (13.00 ± 2.52, 81.33 ± 0.41 and 76.67 ± 1.20) and 4.5% (11.00 ± 4.51, 85.33 ± 9.88 and 84.00 ± 8.50), respectively, after thawing. SL at 0% had the highest (P < 0.05) values for MDA, cholesterol and seminal arginase activity (1.10 ± 0.008 nmol/ml, 236.35 ± 4.08 mg/dl and 0.54 ± 3.3 E-3 units/mg protein, respectively). Our data suggest that 1.5% sunflower lecithin can be used in place of soy lecithin as a substitute for egg yolk during the cryopreservation of caprine semen.

scholarly journals The Quality of Frozen-thawed Canine Semen With Respect to Semen Extender Composition and Sequence of Ejaculate Collection in Dogs

2016 ◽  
Vol 64 (1) ◽  
pp. 169-175
Author(s):  
Jiří Šichtař ◽  
Ondřej Šimoník ◽  
Petra Folková ◽  
Adéla Dokoupilová ◽  
Radko Rajmon ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Computer Assisted ◽  
Time Interval ◽  
Sperm Analysis ◽  
Semen Collection ◽  
Movement Characteristics ◽  
Semen Extender

The aim of this study was to evaluate the effect of clarified egg yolk addition to semen extender, and the semen collection sequence on the quality of frozen-thawed semen in dogs. Semen was collected from 6 dogs in a time interval of 24 hours. As parameter of the quality of frozen-thawed (F-T) semen, the motility by computer assisted sperm analysis (CASA) and plasma membrane integrity by hypo-osmotic swelling test (HOS) were evaluated. All kinematic parameters of sperm motility were higher in F-T samples containing the whole in comparison to the clarified egg yolk. The sequence of semen collection affected sperm movement characteristics of native as well as F-T semen, but it was not possible to determine whether the fresh semen from the 1st or 2nd collection is of higher quality. All motility parameters of sperms frozen with extender containing the whole egg yolk were significantly higher in the case of the 2nd collection. The situation was not so clear in the case of clarified egg yolk addition, but the velocity values were higher in F-T samples from the 2nd collection. In contrast to proven differences in motility, the effect of the addition of clarified egg yolk and the sequence of semen collection were not projected at all on the quality of plasma membrane of canine sperms evaluated by HOS test.

scholarly journals INCLUSION OF PROPOLIS IN RABBIT DIETS AND SEMEN CHARACTERISTICS

2015 ◽  
Vol 17 (4) ◽  
Author(s):  
Gentil Vanini de Moraes ◽  
Marcela Mataveli ◽  
Lucimar Pontara Peres de Moura ◽  
Cláudio Scapinello ◽  
Freddy Mora ◽  
...  
Keyword(s):  
Linear Models ◽  
Semen Quality ◽  
White Male ◽  
The Other ◽  
Small Reduction ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Negative Effect ◽  
Different Levels ◽  
Artificial Vagina

This experiment was carried out with the objective of evaluating different levels of powder propolis in rabbit diets and their effect on semen characteristics. A total of 36 New Zealand White male rabbits were used, randomly distributed into six groups, corresponding to six propolis levels (0, 0.25, 0.50, 0.75, 1.0 and 1.25 g propolis/kg of ration). Semen was collected twice a week, using an artificial vagina. Semen volume, progressive spermatic motility, spermatic vigor, spermatic concentration and spermatic morphology were analyzed. General linear models were used for statistical analysis. The inclusion of powder propolis in the diet increased normal spermatozoa percentage and reduced spermatozoa abnormalities. The powder propolis did not affect the progressive spermatic motility, spermatic vigor or spermatic concentration. The values were considered normal for rabbits. However, a small reduction in semen volume was observed, without any negative effect on the other semen characteristics evaluated. Thus, it is possible to observe better semen quality with the inclusion of 1.25 g powder propolis/kg in the diet for reproducer rabbits.

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