scholarly journals 81 EFFECT OF SUCROSE CONCENTRATION IN WARMING MEDIUM ON THE DEVELOPMENT POTENTIAL OF VITRIFIED BOVINE OOCYTES

2005 ◽  
Vol 17 (2) ◽  
pp. 190
Author(s):  
W.C. Chang ◽  
J. Xu ◽  
S. Jiang ◽  
X.C. Tian ◽  
X. Yang ◽  
...  
Keyword(s):  
Sucrose Concentration ◽  
Control Group ◽  
Bovine Oocyte ◽  
Cell Stage ◽  
Significant Difference ◽  
Humidified Air ◽  
Bovine Oocytes ◽  
Experimental Group ◽  
One Way Anova

The aim of this experiment was to determine the effect of the sucrose concentration (0 to 0.33 M) in the dilution medium on the viability, fertilizability, and development of vitrified bovine oocytes. Bovine oocyte-cumulus complexes were collected from slaughterhouse ovaries and in vitro-matured as reported previously. After 24-h maturation in TCM199-based medium under 5% CO2 humidified air at 39°C, these were exposed to hyaluronidase and carefully pipetted to remove all except the 3–5 innermost layers of cumulus. Oocytes were put into the pre-equilibration medium for 3 min and then into vitrification solution containing HEPES-buffered TCM199 supplemented with 20% FBS, ethylene glycol, and dimethylsulphoxide for 25–30 s; they were then vitrified by modified solid surface vitrification (Dinnyes et al. 2000 Biol. Reprod. 63, 513–518).The oocytes were warmed at 39°C by placing them in holding medium with 0, 0.08, 0.17, 0.25, or 0.33 M sucrose. Non-vitrified oocytes were used as controls. Oocytes were inseminated 30 min after warming, and the presumptive zygotes were cultured in CR1-aa medium supplemented with 6 mg/mL BSA at 39°C in a humidified atmosphere of 5% CO2, 5% O2, and 90% N2 for eight days. Data were analyzed by one-way ANOVA. As shown in Table 1, there was no significant difference in survival rate (P > 0.05) of the vitrified oocytes that were placed in dilution solution containing 0.17, 0.25, or 0.33 M sucrose and the non-treated controls. On Day 2 (fertilized on Day 0), cleavage to the 8-cell stage was similar for the 0.17, 0.25, and 0.33 M dilution groups, but the rates for all three were significantly lower (P < 0.05) than for the control group. The blastocyst rate on Day 8 was significantly higher for the 0.25 M group than for any other experimental group but still significantly lower than for the control. In conclusion, this study suggests that with this vitrification/warming procedure the optimum concentration of sucrose in the dilution solution is 0.25 M. Table 1. Oocyte survival after vitrification/warming and subsequent embryo development The authors would like to thank Ms Colleen Shaffer for the preparation of bovine oocytes.

H1foo is essential for in vitro meiotic maturation of bovine oocytes

Zygote ◽  
2014 ◽  
Vol 23 (3) ◽  
pp. 416-425 ◽  
Author(s):  
Yan Yun ◽  
Peng An ◽  
Jing Ning ◽  
Gui-Ming Zhao ◽  
Wen-Lin Yang ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Polar Body ◽  
Linker Histone ◽  
Meiotic Maturation ◽  
Control Group ◽  
Bovine Oocyte ◽  
First Polar Body ◽  
Effective Sirnas ◽  
Bovine Oocytes

SummaryOocyte-specific linker histone, H1foo, is localized on the oocyte chromosomes during the process of meiotic maturation, and is essential for mouse oocyte maturation. Bovine H1foo has been identified, and its expression profile throughout oocyte maturation and early embryo development has been established. However, it has not been confirmed if H1foo is indispensable during bovine oocyte maturation. Effective siRNAs against H1foo were screened in HeLa cells, and then siRNA was microinjected into bovine oocytes to down-regulate H1foo expression. H1foo overexpression was achieved via mRNA injection. Reverse transcription polymerase chain reaction (RT-PCR) results indicated that H1foo was up-regulated by 200% and down-regulated by 70%. Based on the first polar body extrusion (PB1E) rate, H1foo overexpression apparently promoted meiotic progression. The knockdown of H1foo significantly impaired bovine oocyte maturation compared with H1foo overexpression and control groups (H1foo overexpression = 88.7%, H1foo siRNA = 41.2%, control = 71.2%; P < 0.05). This decrease can be rescued by co-injection of a modified H1foo mRNA that has escaped from the siRNA target. However, the H1e (somatic linker histone) overexpression had no effect on PB1E rate when compared with the control group. Therefore we concluded that H1foo is essential for bovine oocyte maturation and its overexpression stimulates the process.

scholarly journals 271 ASSESSMENT OF NUCLEAR STATUS OF ACTIVATED BOVINE OOCYTES MATURED IN DIFFERENT MATURATION CONDITIONS IN VITRO

2005 ◽  
Vol 17 (2) ◽  
pp. 285
Author(s):  
J.I. Park ◽  
Y. Jang
Keyword(s):  
Oocyte Maturation ◽  
Polar Body ◽  
Calf Serum ◽  
Chi Square ◽  
Cell Stage ◽  
Maturation Medium ◽  
Second Polar Body ◽  
Humidified Air ◽  
Bovine Oocytes

This study was carried out to assess the nuclear status after parthenogenetic activation in in vitro matured oocytes under different conditions. Bovine ovaries were collected from slaughtered cows at a local abattoir. Oocytes were aspirated from follicles of 3–8 mm in diameter and transferred to maturation medium: tissue culture medium (TCM)-199 supplemented with 10% (v/v) fetal calf serum, 100 mg/mL l-cysteine, 20 mg/mL sodium pyruvate, gonadotropins (each 250 IU of eCG and hCG/mL), and 10 mg/mL epidermal growth factor, with or without 5 mM hypotaurine and taurine. Oocytes were cultured at 38.9°C in 5% CO2 in humidified air. After 24 h of culture, oocytes with polar body were selected and submitted to activation treatments. Oocytes were exposed to calcium ionomycin (5 μM for 5 min) followed by incubation with 6-DMAP (2 mM), roscovitine (50 μM), or 6-DMAP + roscovitine for 3.5 h. After activation, oocytes were cultured in mSOF medium containing 0.8% BSA at 38.9°C in 5% CO2, 5% O2 in humidified air for 16 h and stained with Hoechst 33342 or aceto-orcein for assessment of nuclear status. Nuclear status was recorded as follows: 1PB (polar body) + 1PN (pronucleus), 2PB + 1PN and others. Data were analyzed using chi-square test. The maturation rate of bovine oocytes cultured in maturation medium containing hypotaurine/taurine (89.3%, n = 84) was higher (P < 0.05) than those cultured without hypotaurine/taurine (72%, n = 93). In the oocytes matured with hypotaurine/taurine, the rates of diploid activation (1PB + 1PN) were 84% (n = 50) in oocytes treated with 6-DMAP + roscovitine, 78.6% (n = 56) with 6-DMAP, and 52% (n = 50) with roscovitine. In the oocytes matured without hypotaurine/taurine, the rates of diploid activation were 80% (n = 60) in oocytes treated with 6-DMAP + roscovitine, 72% (n = 50) with 6-DMAP, and 54% (n = 50) with roscovitine. The rates of diploid activation were not different in oocytes matured with or without hypotaurine/taurine and among activation treatments. The oocytes treated with roscovitine showed a lower rate (P < 0.05) of diploid activation and higher rate (39.3–40%) of second polar body extrusion (1PN + 2PB) than the other activation groups in both maturation conditions. Cleavage rates to 2-cell stage were 40–45% in all groups. Development rate of blastocysts were 7–10% in all the groups treated with 6-DMAP and 6-DMAP + roscovitine and no blastocysts were obtained from the groups treated with roscovitine alone. Hypotaurine/taurine are known to be stable and potent antioxidants, and have shown the properties of supporting oocyte maturation and further embryonic development (Guerin and Menezo 1995 Zygote 3, 333–43; Mizushima and Fukui 2001 Theriogenology 55, 1432–45). In this study, although the effectiveness of hypotaurine/taurine on promoting oocyte maturation was observed, there were no significant improvements in the rate of diploid activation in oocytes matured with hypotaurine/taurine. These results suggest that the nuclear status of activated oocytes may not have a direct relationship with the enhanced maturation condition. This work was supported by BioGreen 21 Program(#1000520030100000-1), Republic of Korea.

315 SELECTION OF BOVINE OOCYTES BY BRILLIANT CRESYL BLUE BEFORE IN VITRO MATURATION IMPROVES BLASTOCYST DEVELOPMENT

2007 ◽  
Vol 19 (1) ◽  
pp. 273 ◽  
Author(s):  
A. Sugulle ◽  
S. Katakawa ◽  
S. Yamamoto ◽  
S. Oomori ◽  
I. Itou ◽  
...  
Keyword(s):  
Embryo Development ◽  
In Vitro Maturation ◽  
Control Group ◽  
Blastocyst Development ◽  
Cell Stage ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Bovine Oocytes ◽  
Selection Of

The morphological identification of immature oocytes has commonly been used to select the bovine oocytes for IVF. However, &lt;30% of the recovered oocytes reach the blastocyst stage after fertilization, and this is probably due to the quality of the oocytes at the beginning of maturation. The brilliant cresyl blue (BCB) stain determines the activity of glucose-6-phosphate dehydrogenase, an enzyme synthesized in growing oocytes. The aim of this study was to evaluate the effect of the BCB stain on the selection of bovine oocytes and on the subsequent embryo development for in vitro production (IVP). Cumulus–oocyte complexes (COCs) were collected by the aspiration of 2- to 6-mm follicles. A total of 559 oocytes were divided into 2 groups: (1) a control group, immediately cultured, and (2) a BCB-incubated group. After 90 min of BCB staining (Pujol et al. 2004 Theriogenology 61, 735–744), the oocytes were divided into oocytes with blue cytoplasm (BCB+) and oocytes without blue cytoplasm (BCB−). The COCs were matured for 20 h in TCM-199 supplemented with 5% calf serum (CS) and 0.02 mg mL−1 FSH at 38.5°C under an atmosphere of 5% CO2 in air. The matured COCs were inseminated with 5 × 106 sperm mL−1. After 18 h of gamete co-culture, the presumed zygotes were cultured in CR1aa supplemented with 5% CS for 9 days at 38.5°C under an atmosphere of 5% CO2, 5% O2, and 90% N2. Embryonic development was evaluated at 48 h after IVF (proportion of ≥5-cell stage, the total cleavage rates) and on Days 7 to 9 (blastocyst rate). The experiment was replicated 5 times, and the data were analyzed by a chi-square test and ANOVA. The results are presented in Table 1. The proportion of embryos with ≥5-cell stage was significantly higher (P &lt; 0.01) in the BCB+ group than in the BCB− group, but not in the control group. The total cleavage rate for the BCB+ embryos was significantly higher than that of either the BCB− or the control group (P &lt; 0.01). There were also significant differences (P &lt; 0.01) in the blastocyst development between the BCB+ and BCB− embryos and between the BCB− and the control embryos (P &lt; 0.05). This result showed that the selection of bovine oocytes by BCB staining before in vitro maturation may be useful for selecting oocytes that are developmentally competent up to Day 9 for IVP. Table 1.Effect of selection of oocytes by brilliant cresyl blue (BCB) staining on the subsequent embryo development of in vitro-matured/in vitro-fertilized bovine embryos

Comparison of the Initial Repeated Bond Strength among Three Orthodontic Bonding Systems

2014 ◽  
Vol 1025-1026 ◽  
pp. 385-390
Author(s):  
Niwat Anuwongnukroh ◽  
Surachai Dechkunakorn ◽  
Jirawat Arunakol ◽  
Wassana Wichai
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Tooth Movement ◽  
Testing Machine ◽  
Control Group ◽  
Instron Testing Machine ◽  
Significant Difference ◽  
Bonding Systems ◽  
Experimental Group

One of the problems that often occurred during orthodontic treatment is bracket failure. This is usually the result either of the patient’s accidentally, applying inappropriate forces to the bracket or of a poor bonding technique. Thus, a significant number of teeth have to be rebonded in an orthodontic practice. Objective: The aim of this study was to evaluate the in vitro initial repeated shear bond strength of the three adhesive systems at two and five minutes after placement of a bracket. Materials and Methods: The three bonding agent adhesives are System1+, Rely-a-bond, Unite. Two hundred and forty human premolar teeth were divided into two groups, a control and an experimental group. Each group was further divided into three subgroups for bonding brackets with the three different adhesives. Only the teeth in the experimental group were sequentially bonded and debonded two times with the same adhesive. The teeth in control and experimental groups were tested for shear bond strength (at two and five minutes after the bracket was bonded) with an Instron testing machine. Results: The studies were found that : (1) there were differences between the shear bond strength of each adhesive in the control and experimental group. Unite had the highest shear bond strength followed by Rely-a-bond and System1+ at two minutes and five minutes, (2) the experiment group ( rebonded brackets) had higher shear bond strength than control group and Unite had in significant difference (p<0.05) of initial repeated bond strength with System1+ and Rely-a-bond at two minutes and five minutes and (3) there were mostly significant difference (p<0.05) between repeated shear bond strength at two minutes and repeated shear bond strength at five minutes. Conclusion: There were significant difference of the initial repeated shear bond strength of each adhesive. The orthodontists should be aware of applying force for tooth movement into the repeated bonding brackets.

scholarly journals Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines

2019 ◽  
Vol 71 (5) ◽  
pp. 1433-1444 ◽  
Author(s):  
B.B. Santana ◽  
G.G. Sobral ◽  
E.T. Gomes ◽  
A.M. Batista ◽  
L.P.R. Teixeira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Control Group ◽  
Cleavage Rate ◽  
Time Control ◽  
Follicular Aspiration ◽  
Maturation Medium ◽  
Significant Difference ◽  
Group A ◽  
Bovine Oocytes

ABSTRACT The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated shortly after follicular aspiration, 79.00% were in GV, GVBD, MI, while 13.40%, were in MII and 7.60%, D/NI. Significant difference was observed in different concentrations (T100, T200 and T150µM) in oocytes that have reached the MII phase compared to control treatments (P= 0.003). Differences were observed in cleavage rate (P< 0.05) between T150 and T200 when compared to the C/24 Group. A high difference was observed on blastocyst rate (P< 0.001) among treatments compared to the control group.

scholarly journals The In Vitro Anti-Diabetic Activity of Lime Peels (Citrus amblycarpa (Hassk.) Ochse)

2020 ◽  
Vol 13 (01) ◽  
pp. 26-33
Author(s):  
Gempita Cahya aulia tambunan ◽  
Aparna Dutt ◽  
Sayra Nadhifa ◽  
Firdha Amelia ◽  
Ermi Girsang
Keyword(s):  
Control Group ◽  
Control Group Design ◽  
Phytochemical Content ◽  
Ic50 Value ◽  
Significant Difference ◽  
Post Test ◽  
Lime Peel ◽  
Post Hoc ◽  
One Way Anova

There are various potential natural anti-diabetic drugs; one of them is lime peel or Citrus amblycarpa. This study was aimed to explore the anti-diabetic activity and phytochemical content of lime peels. This study was an experimental study that used the post-test only control group design. The lime peels that were collected from the Berastagi fruit market in Medan, North Sumatera were extracted using 70% ethanol by maceration methods. The phytochemical screening identified the presence of phenolic, steroid/triterpenoid, terpenoid, saponin, flavonoid, tannin, and alkaloid. Meanwhile, the anti-diabetic activity of lime peels was evaluate using the α-glucosidase enzyme that was gotten from Saccharomyces cerevisiae by α-glucosidase enzyme inhibition methods. Percent of inhibition was express as Mean ± SD and analyzed by One Way ANOVA, Tukey HSD Post Hoc Test, and followed by linear regression. The result of this study showed that there is a significant difference in percentage inhibition α-glucosidase enzyme in each concentration, and it had an IC50 Value amount of 125.93 ± 9.14 µg/mL. The phytochemical content of the lime peels was flavonoid, phenol, steroid/triterpenoid, and alkaloid. Hence, the lime peel has anti-diabetic activity by inhibition of the α-glucosidase enzyme.

scholarly journals THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Dhesy Kartikasari ◽  
Sri Mulyati ◽  
Suzanita Utama ◽  
Pudji Srianto ◽  
Widjiati Widjiati ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Immunocytochemical Staining ◽  
Control Group ◽  
Bovine Oocyte ◽  
Significant Difference ◽  
In Vitro Oocyte Maturation ◽  
Positive Results

This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.

scholarly journals PENGARUH WAKTU PENGADUKAN TERHADAP WAKTU GELASI BAHAN CETAK ALGINAT

2016 ◽  
Vol 4 (1) ◽  
pp. 1-7
Author(s):  
Audia Tria Putri ◽  
Rizanda Machmud ◽  
Murniwati Murniwati
Keyword(s):  
Experimental Method ◽  
Mixing Time ◽  
Setting Time ◽  
Dental Materials ◽  
Control Group ◽  
Anova Test ◽  
Significant Difference ◽  
Experimental Group ◽  
One Way Anova

Alginate is one of many dental materials that used for the impressing materials. Mixing time is one of factorsthat affected the setting time. The aim of this study was to determine the effect of mixing time at 30 seconds, 35seconds, 40 seconds, 45 seconds, 50 seconds, 55 seconds and 60 seconds on setting time of alginate. This studyused experimental method. The samples that used were 42 samples and divided into 7 groups of mixing time,that were 30 seconds (control), 35 seconds, 40 seconds, 45 seconds, 50 seconds, 55 seconds, and 60 seconds.Setting time was tested with acrylic test rod based on ADA specification no.18. The alginate powder was mixedwith water (10 gr : 23 ml ratio), then put in the mould. Acrylic test rod was placed in contact with the surface ofalginate dough. The setting time was measured from the starting of the mix to the time when alginate does notadhere to the end of the rod. Result of this study showed that the average of setting time of alginate which mixedfor 30 seconds (control), 35 seconds, 40 seconds, 45 seconds, 50 seconds, 55 seconds, and 60 seconds was120.17 ± 3.312 seconds, 119.00 ± 1.265 seconds, 118.17 ± 1.472 seconds, 114.83 ± 3.896 seconds, 112.00 ±1.673 seconds, 109.17 ± 0.983 seconds, and 105.33 ± 4.082 seconds respectively. One Way Anova test shows significant difference among all experimental group with p=0.000. The change of setting time alginate show significant difference with control group when it mixed for 45 seconds, 50 seconds, 55seconds, and 60 seconds.Keywords: alginate, mixing time, setting time

scholarly journals INFLUENCE OF DIFFERENT BLEACHING SYSTEMS ON THE BOND STRENGTH OF LAMINATE VENEER TO ENAMEL

2018 ◽  
Vol 4 (2) ◽  
pp. 1-6
Author(s):  
Ozgun Yusuf Ozyilmaz ◽  
Filiz Aykent ◽  
Ali Riza Tuncdemir ◽  
Haluk Baris Kara
Keyword(s):  
Bond Strength ◽  
Diode Laser ◽  
In Vitro Study ◽  
Unit Group ◽  
Tooth Surface ◽  
Control Group ◽  
Significant Difference ◽  
And Control ◽  
One Way Anova

Purpose: The objective of this in-vitro study was to examine the microtensile bond strength of a porcelain laminate veneer (PLV)  to tooth surface bleached with photoactivation by blue light-emitting diode (LED) or diode laser. Methods: Eigthteen extracted human central incisors were randomly divided into three groups. Two sticks were obtained from each tooth (n=12). Before surface treatments; teeth were prepared to provide space for PLVs. The first group teeth were bleached with Whiteness HP  which is contain 35% hydrogen peroxide (HP) and then photoactivated with  a LED for 20 seconds. The second  group were bleached with Laserwhite 20  which is contain 46% HP and  photoactivated with a diode laser for 30 seconds. The third group received no surface treatment and served as the control group. IPS Esthetic ceramic veneers were luted with Variolink II veneer cement . The teeth were sectioned to obtain porcelain-resin-enamel/dentin sticks and submitted to a MTB testing device. The maximum load at fracture  was recorded. Data were analyzed using one-way ANOVA followed by the Tukey HSD post-hoc test at a preset  α of  0.05. Results: One-way ANOVA revealed that there was significant difference between LED unit group and control group (p<.05) but  no statistical differences were observed with diode laser group (p>.05)  The LED unit group presented significantly lower bond strength value (6.49±2.3 MPa) than diode laser (8.49±3.1 MPa) and control groups (9.53±2.7 MPa). Conclusion: The results suggested that bleaching therapy with activation by LED or diode laser reduced the bond strength of IPS Esthetic ceramic veneers to tooth surfaces.Keywords: Teeth Bleaching; Photoactivation; Semiconductor lasers; Diode laser; Microtensile.

scholarly journals Healing in Transplanted Teeth with Periodontal Ligament Cultured In Vitro

2003 ◽  
Vol 12 (5) ◽  
pp. 519-525 ◽  
Author(s):  
A. Saito ◽  
E. Saito ◽  
M. Kawanami ◽  
A. Shimada
Keyword(s):  
Tissue Culture ◽  
Connective Tissue ◽  
Periodontal Ligament ◽  
Root Surface ◽  
Periodontal Ligament Cells ◽  
Control Group ◽  
Significant Difference ◽  
Tissue Attachment ◽  
Experimental Group

Regeneration of connective tissue attachment is the ultimate goal of periodontal therapy. It has been suggested that periodontal ligament cells possess the potential to create new connective tissue attachment. However, as cells from gingiva and alveolar bone occupy the root surface during initial wound healing, population by periodontal ligament cells is limited in vivo. We have been developing a new periodontal regeneration technique using in vitro tissue culture of periodontal ligament remaining on a periodontally involved root. The purpose of this study was to examine the periodontal healing after transplantation of teeth with reduced periodontal ligament that had been cultured in vitro. Twenty-five incisors from four beagles were used. After the teeth were extracted, the periodontal ligament and cementum were removed from coronal part of the roots and the roots were planed. The periodontal ligament of the apical part was retained. Fourteen teeth of the experimental group were transplanted following culture for 6 weeks. Eleven teeth of the control group were similarly prepared and immediately transplanted without tissue culture. Four weeks after transplantation, the specimens were prepared for histological analysis. Downgrowth of junctional epithelium on the root of experimental group was significantly less than control. Most of the root planed surfaces of experimental group were covered with periodontal ligament fibers oriented parallel or inclined to the root surfaces and limited new cementum formation was observed near the apical end of the planed root. There was no significant difference between groups in observations on the root surface with remaining periodontal ligament. From the above results, it was concluded that periodontal tissue culture of teeth with root planed surface and remaining periodontal ligament could reduce the extent of epithelium downgrowth and increase connective tissue adhesion on the root planed surface, as well as minimize damage to remaining periodontal ligament, after transplantation of teeth.

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