scholarly journals 5 NEUTRAL SEGREGATION OF DONOR CELL MITOCHONDRIA IN FETAL AND ADULT TISSUES OF SOMATIC CELL CLONES IN CATTLE

2005 ◽  
Vol 17 (2) ◽  
pp. 153
Author(s):  
F. Viramontes ◽  
F. Filion ◽  
L.C. Smith
Keyword(s):  
Blood Cells ◽  
Bos Taurus ◽  
White Blood Cells ◽  
Bos Indicus ◽  
Pcr Amplification ◽  
Donor Cell ◽  
Wild Type ◽  
Mtdna Mutation ◽  
D Loop ◽  
The Mean

Until now, animal cloning has been extremely inefficient: only 1–2% of nuclear transfer (NT) clones survive to birth. Some of these anomalies may be related to an incompatibility between nuclear and mitochondrial genes (Cummins JM 2001 Hum. Reprod. Update 7, 217–228). Controversy exists as to the levels of donor cell mitochondrial DNA (mtDNA) inheritance in somatic clones (heteroplasmy). Whereas some researchers found very low quantities (0.1–4%) (Steinborn R et al. 2000 Nat. Genet. 25, 255–257), others found levels of heteroplasmy ranging from 6 to 40% (Takeda et al. Mol. Reprod. Dev. 64, 429–437). Since it remains unclear whether mtDNA segregation is neutral or selective, the purpose of this study was to analyze the transmission of the mtDNA from donor somatic cells in fetal and adult clones using a particular mtDNA marker (mtDNA Bos taurus with one mutation in the D-loop of 40 base pairs plus than the wild type). Fibroblasts from a fetus of 60 days were used as donor cells. The fetus was produced by artificial insemination of a Holstein (Bos taurus) heifer carrying an mtDNA mutation with semen from a Zebu (Bos indicus) bull. Oocytes derived from slaughterhouse ovaries of Holstein cows carrying wild-type mtDNA were used as recipient cells. The presence of the mutated mtDNA from the donor cell (heteroplasmy) was analyzed in a male cloned fetus of 60 days and in three adult male clones at 18 months of age. Heteroplasmy was detected in 7 tissues in the foetus: muscle, skin, stomach, testicle, thymus, tongue, and umbilical cord. Three tissues were analyzed from the adult clones: semen, skin, and white blood cells. Heteroplasmy was detected in all the tissues by nested PCR amplification of the D-loop and analyzed by ANOVA and Tukey-Kramer multiple comparison test. The mean (%) of the mutated mtDNA of the donor cell in the seven tissues of the60-day-old fetus was 1.14 ± 0.34 (SEM). There was no differences in the means of heteroplasmy (%) between the tissues of the fetus (P > 0.05). The mean level of heteroplasmy in the three adult clones analyzed (clones A, B, and C) was 1.41 ± 0.18 (SEM). Analysis of heteroplasmy between the tissues of each clone showed no differences (P > 0.05) with the exception of clone B, where semen was different (P < 0.05) from white blood cells. There were significant differences (P < 0.05) between some clones (taking together all the results of all tissues of each clone). The heteroplasmy in clone B (%) (2.59 ± 0.18 SEM) was different (P < 0.05) from that of both clone A (1.04 ± 0.18) and clone C (1.46 ± 0.18). There was no difference between the heteroplasmy (%) of clone A and that of clone C (P > 0.05). These results show that the tissues of the fetus and the adult clones were heteroplasmic at similar levels, suggesting neutral segregation of the donor cell mtDNA during development and tissue differentiation.

scholarly journals Adult Mice With Targeted Mutation of the Interleukin-11 Receptor (IL11Ra) Display Normal Hematopoiesis

Blood ◽  
1997 ◽  
Vol 90 (6) ◽  
pp. 2148-2159 ◽  
Author(s):  
Harshal H. Nandurkar ◽  
Lorraine Robb ◽  
David Tarlinton ◽  
Louise Barnett ◽  
Frank Köntgen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Bone Marrow Cells ◽  
White Blood Cells ◽  
Null Mutation ◽  
Wild Type ◽  
Normal Numbers ◽  
Interleukin 11 ◽  
Marrow Cells

Abstract Interleukin-11 (IL-11) is a pleiotropic growth factor with a prominent effect on megakaryopoiesis and thrombopoiesis. The receptor for IL-11 is a heterodimer of the signal transduction unit gp130 and a specific receptor component, the α-chain (IL-11Rα). Two genes potentially encode the IL-11Rα: the IL11Ra and IL11Ra2 genes. The IL11Ra gene is widely expressed in hematopoietic and other organs, whereas the IL11Ra2 gene is restricted to only some strains of mice and its expression is confined to testis, lymph node, and thymus. To investigate the essential actions mediated by the IL-11Rα, we have generated mice with a null mutation of IL11Ra (IL11Ra−/−) by gene targeting. Analysis of IL11Ra expression by Northern blot and reverse transcriptase-polymerase chain reaction, as well as the absence of response of IL11Ra−/− bone marrow cells to IL-11 in hematopoietic assays, further confirmed the null mutation. Compensatory expression of the IL11Ra2 in bone marrow cells was not detected. IL11Ra−/− mice were healthy with normal numbers of peripheral blood white blood cells, hematocrit, and platelets. Bone marrow and spleen contained normal numbers of cells of all hematopoietic lineages, including megakaryocytes. Clonal cultures did not identify any perturbation of granulocyte-macrophage (GM), erythroid, or megakaryocyte progenitors. The number of day-12 colony-forming unit-spleen progenitors were similar in wild-type and IL11Ra−/− mice. The kinetics of recovery of peripheral blood white blood cells, platelets, and bone marrow GM progenitors after treatment with 5-flurouracil were the same in IL11Ra−/− and wild-type mice. Acute hemolytic stress was induced by phenylhydrazine and resulted in a 50% decrease in hematocrit. The recovery of hematocrit was comparable in IL11Ra−/− and wild-type mice. These observations indicate that IL-11 receptor signalling is dispensable for adult hematopoiesis.

scholarly journals Effect of hormonal growth promotants on palatability and carcass traits of various muscles from steer and heifer carcasses from a Bos indicus - Bos taurus composite cross

2008 ◽  
Vol 48 (11) ◽  
pp. 1415 ◽  
Author(s):  
R. Watson ◽  
R. Polkinghorne ◽  
A. Gee ◽  
M. Porter ◽  
J. M. Thompson ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Vastus Lateralis ◽  
Carcass Traits ◽  
Bos Indicus ◽  
Rectus Femoris ◽  
Biceps Femoris ◽  
Cooking Methods ◽  
Triceps Brachii ◽  
The Mean ◽  
Psoas Major

The effect of several different hormonal growth promotant (HGP) implant strategies on the palatability and carcass traits of different muscles in beef carcasses was investigated using samples from heifer and steer carcasses from a Bos indicus composite breed. In experiment 1, there were seven different implant strategies evaluated in heifers that were given different combinations of up to three implants (implanted at weaning, during backgrounding and at feedlot entry). A total of 112 heifers were slaughtered and 11 muscles or portions were collected from both sides [Mm. adductor femoris, gracilus, semimembranosus, longissimus dorsi lumborum, triceps brachii caput longum, semispinalis capitis, serratus ventralis cervicis, spinalis dorsi, biceps femoris (syn. gluteobiceps), tensor fasciae latae, gluteus medius (both the ‘D’ and the ‘eye’ portions) rectus femoris, vastus intermedius, vastus lateralis and vastus medialis]. These muscles were used to prepare a total of 1030 sensory samples which were aged for either 7 or 21 days and frozen. Thawed samples were cooked using different cooking methods (grill, roast and stir frying) before being evaluated by a consumer taste panel that scored samples for tenderness, juiciness, like flavour and overall liking. Experiment 2 used the steer portion from the same calving, which were treated to a similar array of HGP strategies, except that they were given up to four implants between weaning and slaughter at ~3 years of age. In experiment 2, there was a total of 12 different HGP implant strategies tested. At boning, three muscles (Mm. psoas major, longisimuss dorsi thoracis and lumborum portions) were collected from each of 79 carcasses with a total of 237 steak samples that consumers tested as grilled steaks. For both experiments, the mean of the HGP implant strategies resulted in increased ossification scores (P < 0.05) and decreased marbling scores (P < 0.05) compared with the controls, with the effect on ossification being much larger in the older steer groups. In both experiments, the different HGP strategies decreased (P < 0.05) all sensory scores compared with the controls, for all cooking method and muscle combinations. In experiment 1, there was no interaction between the mean HGP effect and muscle (P > 0.05), and aging rates differed among the muscles (P < 0.05). In experiment 2, there was a significant (P < 0.05) muscle × HGP treatment interaction, with a decrease in tenderness score due to HGP implant strategies in the M. longisimuss thoracis and lumborum portions, compared with no significant effect in the M. psoas major. For both experiments, there were no significant differences among the different implantation strategies on sensory scores (P > 0.05).

The thickness of cattle skin

1955 ◽  
Vol 6 (5) ◽  
pp. 776 ◽  
Author(s):  
DF Dowling
Keyword(s):  
Bos Taurus ◽  
Bos Indicus ◽  
Sweat Glands ◽  
Skin Thickness ◽  
Hot Environment ◽  
Early Maturing ◽  
The Mean ◽  
Critical Characteristics ◽  
Thick Skin ◽  
Active Sweat Glands

The mean total skin thicknesses (mm) for the breeds sampled were: Devon 8.15, Hereford 6.7, Zebu cross 6.43, Australian Illawarra Shorthorn 6.23, Friesian 6.0, Zebu 5.77, Aberdeen Angus 5.75, Shorthorn 5.69, and Jersey 5.46. The early maturing Shorthorn (Bos taurus L.) and the Zebu (Bos indicus L.) differed significantly in the depth of the papillary and reticular layers, and in the relative thickness of these skin layers. The mean thickness for the papillary layer; was 0.98 mm in the Zebu, 1.40 mm in the Zebu cross, and 1.70 mm in the Shorthorn. Thus the larger, more active sweat glands of the Zebu are more superficial. The corresponding reticular layer averaged 4.45 mm in the Zebu, 4.5 mm in the Zebu cross, and 4.08 mm in the Shorthorn. The thickness of the papillary layer relative to the total skin thickness was 18.3, 23.7, and 29.3 per cent., for the Zebu, Zebu cross, and Shorthorn respectively. The Zebu and the Jersey have relatively thin skins. Therefore a thick skin is not essential for adaptability to a hot environment. Inherent differences in the function of the follicles and the glands of the papillary layer may be critical characteristics for heat tolerance.

Evaluation of Bos indicus and Bos taurus straightbreds and crosses. II. Post-weaning growth, puberty, and pelvic size of heifers

1994 ◽  
Vol 45 (4) ◽  
pp. 795 ◽  
Author(s):  
H Hearnshaw ◽  
PF Arthur ◽  
R Barlow ◽  
PJ Kohun ◽  
RE Darnell
Keyword(s):  
Body Condition ◽  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
The Other ◽  
South Wales ◽  
Condition Score ◽  
Medium Quality ◽  
The Mean ◽  
Pelvic Size

Post-weaning growth and body condition, puberty and pelvic size of 197 heifers comprising straightbred Hereford (HxH) and Brahman (BxB), first-cross (BxH) and back-cross (HxBH and BxBH) heifers were evaluated. The heifers were born over a 3 year period, and grazed improved and semi-improved pastures following weaning at Grafton, New South Wales. Prior to weaning, heifers had been reared by dams on three pasture systems (high, medium and low quality pastures). Heifers from low quality pre-weaning pasture had higher (P < 0.05) post-weaning liveweight gain than those from high and medium quality pastures. BxH heifers gained 71 g/day more (P < 0.05) than the mean gain of their contemporaries of the other genotypes, whose gains were similar, from weaning to either 26 or to 30 months of age. Liveweight at all ages was influenced by genotype x pre-weaning pasture system interaction. At 30 months of age, BxH heifers from high and medium pre-weaning pastures were the heaviest. At the same age, but from low quality pre-weaning pasture, heifers with crossbred dams (HxBH and BxBH) were the heaviest. Wither height depended significantly (P < 0.05) on the proportion of Bos indicus genes, increasing from 113.8 cm in the HxH heifers to 124.4 cm in the BxB heifers at 30 months of age. BxH heifers had a higher (P < 0.05) body condition score than their contemporaries of the other genotypes, which were in similar condition, at 26 and 30 months of age. On average (across pre-weaning pasture system), 9% of BxB heifers had reached puberty by 22 months of age compared to 62, 95, 82 and 64% (s.e.= 9) for HxH, HxBH, BxH and BxBH heifers respectively. No significant genotype differences were obtained in the height, width and size of the pelvic opening of the heifers, measured just prior to the beginning of the mating season at 26 months of age.

Comparative drought resistance of Bos indicus and Bos taurus crossbred herds in central Queensland. 2. Relative mortality rates, calf birth weights, and weights and weight changes of breeding cows

1973 ◽  
Vol 13 (61) ◽  
pp. 117 ◽  
Author(s):  
JE Frisch
Keyword(s):  
Weight Loss ◽  
Birth Weight ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Mortality Rates ◽  
Weight Changes ◽  
Method Of Least Squares ◽  
The Mean ◽  
Mature Weight

The mortality rates, weight changes and associated effects of a. draught in 1969 were observed in a herd of Shorthorn x Hereford (SH), Brahman x SH (BX) and Africander x SH (AX) crossbred cows and small herds of purebred Brahman and Africander cows at 'Belmont', Queensland. About half of the cows calved over a two month period from mid-October 1969. All the cows were weighed in February 1968, February and October 1969 and in January 1970 and the data from the crossbreds analysed by the method of least squares. The weights and weight changes of both the calving and non-calving groups followed a similar pattern though the weight changes of the non-calving group were of greater magnitude. The weights showed that the three crossbreds reached the same mature weight but at different rates. The SH cows reached mature weight least rapidly, had the highest mean weight loss during the drought and had the lowest mean gains after the season broke. Weight changes both during and after the drought were however dependent on lactational status in February 1969. The mean weight loss of cows that weaned a calf in 1969 (wet) was similar for all breeds. However, the mean loss of the dry SH cows was significantly greater than that of the dry BX and AX cows. Previously wet cows exhibited compensatory gains after calving but the gain of the wet SH cows was the lowest of the crossbred means. The mean birth weight of the SH calves was reduced below the long term mean by about 4 kg but birthweights of BX and AX calves were similar to those in other years. During the drought the purebred Brahmans lost the least and the purebred Africanders lost the most weight of all breeds. However, the nature of the data precludes any strict comparisons from being drawn. Mortality rates of breeding cows were recorded from 1960 to 1969. Rates were generally highest for the SH, particularly in the drought years of 1965 and 1969. Some reasons are advanced which may explain the differences in performance of the breed types.

179 COMPARISON OF OOCYTE AND EMBRYO PRODUCTION AMONG BOS TAURUS, BOS INDICUS, AND INDICUS-TAURUS DONOR COWS

2010 ◽  
Vol 22 (1) ◽  
pp. 248 ◽  
Author(s):  
J. H. F. Pontes ◽  
K. C. F. Silva ◽  
A. C. Basso ◽  
C. R. Ferreira ◽  
G. M. G. Santos ◽  
...  
Keyword(s):  
High Efficiency ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Cumulus Cells ◽  
Holstein Cows ◽  
Embryo Production ◽  
Total N ◽  
The Mean ◽  
Donor Cows

In recent years, Brazil has become the leading country in the world for the number of embryos produced in vitro (Thibier M 2009 IETS Embryo Transfer Newsletter 22, 12-19). This is partly due to the large numbers of Bos indicus animals in Brazil, making up about 80% of the total cattle. The mean oocyte production per ultrasound-guided follicular aspiration from Bos indicus is higher than those for European breeds (Pontes JHF et al. 2009 Theriogenology 71, 690-697). In the present study, we analyzed 5407 ovum pick ups (OPU) and compared the average production of total (n = 90,086) and viable (n = 64,826) oocytes and the number of embryos produced in vitro from Gir (Bos taurus indicus), Holstein (Bos taurus taurus), 1/4 Holstein × 3/4 Gir, and 1/2 Holstein-Gir crossbreed cows. To obtain oocytes, OPU was repeated from 4 to 7 times (mean = 5.7 ± 2.4) in each donor cow aged from 3 to 7 years (mean = 5.0 ± 2.3) during a 12-mo period. COCs (n = 90,086) obtained were classified according to the presence of cumulus cells and the oocyte cytoplasm aspect (homogeneous or heterogeneous/fragmented). The viable oocytes (n = 64,826) were in vitro matured for 24 h at 38.8°C in an atmosphere of 5% CO2 in air. Since this was a commercial programm, frozen sexed semen (2 × 106 mL-1) from Gir (n = 8) or Holstein (n = 7) sires previously tested for high efficiency was used for IVF. Fertilization was carried out (18-20 h) and the presumed embryos were cultured for 7 days in the same conditions as were used for IVM. Data were analyzed by ANOVA. On average, 16.7 ± 6.2 oocytes were obtained per OPU/IVF procedure and 71.96% were considered viable. The mean numbers of total oocytes per OPU/IVF procedure were 17.1 ± 4.4 for Gir cows (n = 617), 11.4 ± 3.9 for Holstein cows (n = 180), 20.4 ± 5.8 for 1/4 Holstein × 3/4 Gir (n = 44), and 31.4 ± 5.6 for 1/2 Holstein-Gir crossbreed females (n = 37, P < 0.01). The mean numbers of viable oocytes per OPU/IVF procedure were 12.1 ± 3.8 for Gir cows, 8.0 ± 2.6 for Holstein cows, 16.8, ± 5.0 for 1/4 Holstein × 3/4 Gir, and 24.3 ± 4.7 for 1/2 Holstein-Gir crossbreed females (P < 0.01). The average number of embryos produced by OPU/IVF were 3.2 (n = 12,243/3378) for Gir cows, 2.2 (n = 2426/1138) for Holstein cows, 3.9 (n = 1033/267) for 1/4 Holstein × 3/4 Gir, and 5.5 (n = 1222/224) for 1/2 Holstein-Gir. The average number of embryos produced per IVF session from 1/2 taurus × indicus donor cows was greater (P < 0.01) than from Bos indicus cows. The number of recoverable and viable oocytes and the number of embryos produced in vitro from Bos indicus donors were higher than from Bos taurus females. Therefore, the highest oocyte yield and the greatest embryo production were obtained from 1/2 taurus × indicus females. This work was supported by In Vitro Brasil.

Hedgehog Signaling Regulates HSC Proliferation.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 1390-1390
Author(s):  
Akil Merchant ◽  
Giselle Joseph ◽  
William Matsui
Keyword(s):  
Cell Cycle ◽  
Bone Marrow ◽  
Blood Cells ◽  
White Blood Cells ◽  
Wild Type ◽  
Serial Blood ◽  
Myeloid Progenitor ◽  
Hh Signaling ◽  
Progenitor Compartment

Abstract Hedgehog (Hh) signaling is essential for normal development and is dysregulated in many cancers. Hh signaling is active in normal bone marrow and the majority of acute myeloid leukemias, however, the precise role of Hh signaling and its positive effector Gli1 in normal or malignant hematopoiesis is not known. We have analyzed the bone marrow of Gli1 null mice to understand the role of this transcription factor in normal hematopoiesis in order to gain insight into its potential role in leukemia. Gli1 null mice develop normally and have normal peripheral blood counts but the bone marrow shows skewing of the c-Kit+Sca1+Lin-neg (KSL) progenitor compartment with increased CD34negKSL long-term HSC (LT-HSC) and decreased 34+KSL short-term HSC (ST-HSC). An analogous difference was observed in the c-Kit+Sca1negLinneg (KL) myeloid progenitor compartment with an increase in FcRγlowCD34+KL common myeloid progenitors (CMP) and decrease in the FcRγhighCD34+KL granulocyte monocyte progenitors (GMP). We speculated that these differences could be due to impaired cell cycle since both the ST-HSC and GMP are more proliferative than LT-HSC and CMP, respectively. Cell cycle analysis by DNA content and BrdU pulse labeling (100mg/kg IP 14 hours prior to analysis) revealed a marked decrease of proliferation in the LT-HSC, ST-HSC, CMP, and GMP compartments of Gli1 null mice. We supported this conclusion by demonstrating that the bone marrow of Gli1 null mice are relatively radio-resistant. Mice exposed to 400 cGy of total body irradiation followed with serial blood counts revealed less severe nadir, but delayed rebound of white blood cells in Gli1 null mice. We further hypothesized that although Gli1 appears to be dispensable for steady-state peripheral hematopoiesis, it might be necessary for rapid proliferation of progenitors needed during stressed hematopoiesis. In brain development, where Hh signaling is much better understood, active Hh signaling is critical for regulating proliferation of neural stem cells and Gli1 activity significantly increases after depletion of neural progenitors with chemotherapy (Bai et al., Development, 2002). To extend this observation to hematopoiesis, we treated Gli1 null mice and wild-type litter-mates with 5-fluorouracil (5-FU) at 100mg/kg and measured serial blood counts. Gli1 null mice had a delayed recovery of total white blood cells and neutrophil counts at 6 days after 5-FU, but this difference normalized by 20 days after treatment. To confirm that this difference was due to impaired proliferation and not increased sensitivity to 5-FU, we treated Gli1 null and wild-type mice with G-CSF (10mcg/kg/day) for three days to stimulate neutrophil proliferation. Confirming our hypothesis, we observed an attenuated neutrophil response in G-CSF stimulated Gli1 null mice. In summary, we have demonstrated that Gli1 loss leads to decreased HSC and myeloid progenitor proliferation, which has important functional consequences for stress hematopoiesis. These data suggest that abnormal Hh activity in leukemia may be important for driving the uncontrolled proliferation of cancer cells. Gli1 null mice were a kind gift from Alexandra Joyner, Memorial Sloan-Kettering Cancer Center

scholarly journals Utilization of low-quality roughage byBos taurusandBos indicuscattle

1985 ◽  
Vol 53 (3) ◽  
pp. 649-656 ◽  
Author(s):  
R. A. Hunter ◽  
B. D. Siebert
Keyword(s):  
Dry Matter ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Dietary Factors ◽  
Plasma Urea ◽  
Mean Retention Time ◽  
Digitaria Decumbens ◽  
Breed Difference ◽  
Significant Difference ◽  
The Mean

1. In a number of experiments voluntary food intake of three low-quality roughages, either alone or supplemented with rumen-degradable nitrogen and sulphur and minerals, was measured in Brahman (Bos indicus) and Hereford (Bos taurus) steers. The chaffed hays were Spear grass (Heteropogon conform) (6.2 g N/kg organic matter (OM)), Pangola grass (Digitaria decumbens) (7.9 g N/kg OM), and Pangola grass (12.0 g N/kg OM). Rumen characteristics relating to rate of fluid outflow from the rumen were also determined.2. There was no significant difference between breeds in the dry-matter intakes of the unsupplemented diets which ranged from 11.3 to 17.8 g/kg body-weight (BW) by Herefords and from 11.8 to 16.1 g/kg BW by Brahmans.3. Supplementation of Spear grass with N and S significantly (P < 0.05) increased intake by Herefords (24%) but not by Brahmans. When the lower-N Pangola grass was supplemented there was a significant increase in intake by both breeds with the magnitude of the response in Herefords (42%) (P < 0.001) being greater than that in Brahmans (15%) (P < 0.05). The intakes of both the supplemented Spear grass and the lower-N Pangola diets were significantly (P < 0.05) greater by Herefords than Brahmans. There was no breed difference in intake when the higher-N Pangola grass was supplemented. Both breeds recorded an 8% intake response to supplementation, although the increase was only significant (P < 0.05) in Herefords.4. The mean retention time of fluid in the rumen on the unsupplemented Pangola grass diet of lower N content was 12.7 h in Brahmans compared with 17.5 h in Herefords (P < 0.01). When the higher-N Pangola was fed, both alone and supplemented, the mean retention times were similar on both diets (10.5 and 9.9 h for Herefords; 9.5 and 8.1 h for Brahmans for unsupplemented and supplemented diets respectively).5. Plasma urea concentrations were higher in Brahmans than in Herefords on all diets. Rumen ammonia concentrations were significantly (P < 0.001) higher in Brahmans than Herefords when the lower-N Pangola grass diet was unsupplemented.6. The intakes and the variable intake responses to supplementation between breeds and diets are discussed in relation to a number of animal and dietary factors.

Effect of Different Dialyzer Membranes on Serum Angiotensin-Converting Enzyme during Hemodialysis

1988 ◽  
Vol 11 (1) ◽  
pp. 28-32 ◽  
Author(s):  
D. Docci ◽  
C. Delvecchio ◽  
F. Turci ◽  
L Baldrati ◽  
C. Gollini
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Vascular Endothelium ◽  
Blood Cells ◽  
Serum Proteins ◽  
Leukocyte Count ◽  
White Blood Cells ◽  
Converting Enzyme ◽  
Serum Angiotensin Converting Enzyme ◽  
Pulmonary Vascular Endothelium ◽  
The Mean

The effects of different dialyzer membranes on serum concentration of angiotensin-converting enzyme (ACE) and white blood cells during hemodialysis were examined on a cross-over basis in 20 chronically uremic patients. Hemodialysis with cuprophane membranes was associated with a significant (p < 0.001) fall in the mean leukocyte count during the 1st hour of treatment. The use of polymethylmetacrylate membranes resulted in a more attenuated form of leukopenia and with polyacrylonitrile membranes no change was observed during hemodialysis. Hemodialysis with each membrane caused a comparable, significant (p < 0.005) increase in serum ACE, independent of the degree of leukopenia but significantly (p < 0.001) correlated with the increases in serum proteins. We conclude that this increase in serum ACE concentration after hemodialysis does not reflect acute damage of the pulmonary vascular endothelium during treatment and most probably is a result of hemoconcentration. Therefore, serum ACE analysis is not an indicator of dialyzer membrane biocompatibility.

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