scholarly journals 323 THE QUALITY OF PREOVULATORY FOLLICLES DURING FINAL MATURATION IN COWS STIMULATED WITH oFSH AND A DEFINED LH SURGE

2005 ◽  
Vol 17 (2) ◽  
pp. 312
Author(s):  
O. Algriany ◽  
P. Vos ◽  
H. Groenendaal ◽  
A. van Gastel ◽  
B. Colenbrander ◽  
...  
Keyword(s):  
Follicular Development ◽  
High Variability ◽  
Dominant Follicle ◽  
Ultrasound Guided ◽  
Developmental Potential ◽  
Lh Surge ◽  
Final Maturation ◽  
Preovulatory Follicles ◽  
Selection Of

Multiple preovulatory follicles developing upon superovulation (SO) are heterogeneous in quality, which may be the consequence of follicular development deviating from that in untreated cyclic cows. Therefore, we investigated follicle performance in terms of estradiol (E), progesterone (P), and testosterone (T) concentrations in the fluid of stimulated preovulatory follicles (FF), in particular at onset of final maturation as initiated by the LH surge. Pre-synchronized HF cows (n = 25) were treated with oFSH (Ovagen; ICP, Auckland, New Zealand) for SO, and FF were collected 2 h pre-LH surge (n = 9 cows), and 6 h (n = 8) and 22 h C(n = 8) post-LH. At Day 9 (estrus = Day 0), a norgestomet ear implant (Crestar; Intervet International BV, Boxmeer, The Netherlands) was inserted, and SO treatment was started at Day 10 using oFSH i.m. twice daily in decreasing doses during 4 days (total dose 17 mL). Prostaglandin (22.5 mg PG; Prosolvin, Intervet) was administered i.m. concomitant with the 5th dose of FSH. Ear implants were removed 50 h after PG and then GnRH (0.021 mg Receptal; Intervet) was administered i.m. inducing the LH surge 2 h later. Ovaries were collected by laparotomy at the time of GnRH, and 8 and 24 h later and all follicles sized >10 and <16 mm were aspirated to collect FF (pre-LH, n = 79; 6 h post-LH, n = 78; and 22 h post LH, n = 78 follicles). For comparison, E and P in FF from pre-LH groups that had been collected previously in 2 other experiments of our group were studied: (1)86 FF collected by ultrasound-guided aspiration of follicles >8 mm from 23 cows at 30 h after PG, that is, preceding the LH surge, following treatment with 3000 IU eCG i.m. (Folligon; Intervet) on Day 10 and 15 mg PG on Day 12; (2) 12 FF of the dominant follicle from 12 untreated cyclic cows after ovariectomy 48 to 62 h after onset of luteolysis, that is, shortly before the natural LH surge. The concentrations (ng/mL FF) of E, P, and T were estimated by our validated RIAs. Data (mean ± SEM) were analyzed by ANOVA. The levels of E, P and T of the oFSH group were pre-LH: 399 ± 35, 49 ± 6 and 13 ± 2; 6 h post-LH: 194 ± 11, 202 ± 12, and 14 ± 1; and 22 h post-LH: 35 ± 2, 200 ± 23, and 7 ± 1, respectively. Although the change in E and P levels between the different time points after LH is in agreement with that reported for untreated cyclic cows (Dieleman et al. 1983 J.Endocrinol. 97, 31–42), the concentrations were lower. However, the most striking finding was the significantly lower E in pre-LH FF after oFSH compared to that after eCG (1302 ± 82) or of cyclic cows (1942 ± 200). The P levels in FF of the respective pre-LH groups were not significantly different. The much lower E level after oFSH is possibly due to the low or even absent LH bioactivity for oFSH in comparison to eCG. It could also indicate a lower developmental potential of oocytes following oFSH. However, it is known that SO with oFSH results in regular yields of transferable embryos similar to that after eCG. In view of the high variability of the E level in FF, it is concluded that selection of follicles for high E levels is a prerequisite when investigating oocyte development and maturation.

Defining the gonadotrophin requirement for the selection of a single dominant follicle in cattle

2020 ◽  
Vol 32 (3) ◽  
pp. 322 ◽  
Author(s):  
Jin G. Gong ◽  
Bruce K. Campbell ◽  
Robert Webb
Keyword(s):  
Major Effect ◽  
Follicle Development ◽  
Preovulatory Follicle ◽  
Ultrasound Scanning ◽  
Dominant Follicle ◽  
Initial Exposure ◽  
Preovulatory Follicles ◽  
Gonadotrophin Releasing Hormone ◽  
Follicular Response ◽  
Selection Of

The aim was to define the pattern and physiological concentrations of FSH and LH required for the selection of a single dominant follicle in mono-ovulatory species. A series of five experiments was carried out using gonadotrophin-releasing hormone agonist-induced hypogonadal heifers. Animals were infused with different patterns of either FSH and/or LH followed by an ovulatory dose of human chorionic gonadotrophin. Follicular response was monitored by ultrasound scanning and blood samples were collected to measure concentrations of FSH, LH, oestradiol and progesterone. The main findings were: (1) physiological concentrations of FSH given as a continuous infusion and for an adequate duration, in the presence of basal LH, with or without LH pulses, are capable of inducing a superovulatory response, (2) initial exposure to FSH followed by LH pulses alone stimulate the development of multiple preovulatory follicles, confirming that ovarian follicles are capable of transferring dependence on gonadotrophins from FSH to LH, (3) while LH pulses appear not to have a major effect on the pattern of preovulatory follicle development, adequate LH pulsatile support is required for full oestradiol synthesis and (4) the duration of initial exposure to FSH and the ability to transfer the dependence from FSH to LH are critical for the selection of a single dominant follicle. In conclusion, this experimental series confirms that the duration of initial exposure to FSH and the ability of the selected follicle to transfer its gonadotrophic dependence from FSH to LH are critical for the selection of a single dominant follicle in cattle.

scholarly journals Mechanisms regulating follicular development and selection of the dominant follicle

2019 ◽  
Author(s):  
R Webb ◽  
B Nicholas ◽  
JG Gong ◽  
BK Campbell ◽  
CG Gutierrez ◽  
...  
Keyword(s):  
Follicular Development ◽  
Dominant Follicle ◽  
Selection Of

Intranuclear characteristics of pig oocytes stained with brilliant cresyl blue and nucleologenesis of resulting embryos

Zygote ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 232-240 ◽  
Author(s):  
Matej Murin ◽  
Frantisek Strejcek ◽  
Alexandra Bartkova ◽  
Martin Morovic ◽  
Michal Benc ◽  
...  
Keyword(s):  
Blastocyst Stage ◽  
Cell Stage ◽  
Developmental Potential ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Transmission Electron ◽  
Significant Difference ◽  
Chromatin Configuration ◽  
Selection Of

SummaryBrilliant cresyl blue (BCB) vital labelling is a powerful method for analyzing the quality of porcine cumulus–oocyte complexes. Our aim was to investigate the correlation between the selection of porcine oocytes using BCB labelling and selected intranuclear characteristics of porcine oocytes and parthenotes. Moreover, BCB labelling was correlated with the diameter of the oocyte and the developmental potential of the parthenotes. The following methods were used: BCB labelling, measurement of the diameter of the oocyte, parthenogenetic activation, immunocytochemistry, transmission electron microscopy, enucleation and relative protein concentration (RPC) analysis. We determined that the diameter of the oocytes in the BCB-positive (BCB+) group was significantly larger than in the BCB-negative (BCB−) group. Immediately after oocyte selection according to BCB labelling, we found significant difference in chromatin configuration between the analyzed groups. BCB+ oocytes were significantly better at maturation than BCB− oocytes. BCB+ embryos were significantly more competent at cleaving and in their ability to reach the blastocyst stage than BCB− embryos. Ultrastructural analyses showed that the formation of active nucleoli in the BCB+ group started at the 8-cell stage. Conversely, most BCB− embryos at the 8-cell and 16-cell stages were fragmented. No statistically significant difference in RPC in nucleolus precursor bodies (NPBs) between BCB+ and BCB− oocytes was found. We can conclude that BCB labelling could be suitable for assessing the quality of porcine oocytes. Moreover, the evaluation of RPC indicates that the quantitative content of proteins in NPB is already established in growing oocytes.

ON THE EXPERT’S RIGHT TO BE PRESENT AT LEGAL PROCEEDINGS

2016 ◽  
Vol 15 ◽  
pp. 163-171
Author(s):  
M. G. Shcherbakovskiy
Keyword(s):  
Initial Data ◽  
Legal Proceedings ◽  
Reliable Assessment ◽  
Data Acquiring ◽  
Selection Of

The article discusses the reasonsfor an expert to participate in legal proceedings. The gnoseological reason for that consists of the bad quality of materials subject to examination that renders the examination either completely impossible or compromises objective, reasoned and reliable assessment of the findings. The procedural reason consists ofa proscription for an expert to collect evidence himself or herself. The author investigates into the ways of how an expert can participate in legal proceedings. If the defense invites an expert to participate in the proceedings, then it is recommended that his or her involvement should be in the presence of attesting witnesses and recorded in the protocol. In the course of the legal proceedings an expert has the following tasks: adding initial data, acquiring new initial data, understanding the situation of the incident, acquiring new objects to be studied, including samples for examination. An expert’s participation in legal proceedings differs from the participation of a specialist or an examination on the scene of the incident. The author describes the tasks that an expert solves in the course of legal proceedings, the peculiarities ofan investigation experiment practices, the selection of samples for an examination, inspection, interrogation.

Implementasi Voip Server Berbasis IPV6 Dengan Raspberry PI

2019 ◽  
Vol 9 (01) ◽  
pp. 47-54
Author(s):  
Rabbai San Arif ◽  
Yuli Fitrisia ◽  
Agus Urip Ari Wibowo
Keyword(s):  
Internet Protocol ◽  
Wireless Transmission ◽  
Raspberry Pi ◽  
Test Results ◽  
Average Delay ◽  
Ipv6 Network ◽  
Ip Network ◽  
Selection Of ◽  
Better Than

Voice over Internet Protocol (VoIP) is a telecommunications technology that is able to pass the communication service in Internet Protocol networks so as to allow communicating between users in an IP network. However VoIP technology still has weakness in the Quality of Service (QoS). VOPI weaknesses is affected by the selection of the physical servers used. In this research, VoIP is configured on Linux operating system with Asterisk as VoIP application server and integrated on a Raspberry Pi by using wired and wireless network as the transmission medium. Because of depletion of IPv4 capacity that can be used on the network, it needs to be applied to VoIP system using the IPv6 network protocol with supports devices. The test results by using a wired transmission medium that has obtained are the average delay is 117.851 ms, jitter is 5.796 ms, packet loss is 0.38%, throughput is 962.861 kbps, 8.33% of CPU usage and 59.33% of memory usage. The analysis shows that the wired transmission media is better than the wireless transmission media and wireless-wired.

DESIGN, SIMULATION AND IMPLEMENTATION MONITORING SYSTEM FOR SYSTEM CONTROL C4 FEEDING PUMP FOR FEED VAPORIZATION IN THE BUTADIENE PLANT

2020 ◽  
Vol 1 (1) ◽  
pp. 31-35
Author(s):  
Fahmi Yunistyawan ◽  
Yunistyawan J Berchmans ◽  
Gembong Baskoro
Keyword(s):  
Motor Control ◽  
Control System ◽  
Field Operator ◽  
System Control ◽  
Variable Speed ◽  
Variable Speed Drive ◽  
Software And Hardware ◽  
Discharge Pressure ◽  
Selection Of

This study implements the auto start control system on an electric motor 3 phase C4Feeding pump when the discharge pressure is low-low (4.3 kg /cm²). The C4 feeding pumpmotor was initially manually operated from the local control station, this was very ineffectiveand inefficient because it still relied on the field operator to operate the pump motor and whenthe plant was in normal operating it is very risk if the field operator late to operate motor then itwill impact to quality of the product, and if the delay time to operate motor is too long then planthave to shut down, therefore improvement is needed in the C4 feeding pump motor controlsystem. In this paper, various types of 3-phase motor control are explained which allow it to beapplied to the C4 feeding pump motor that are on-off, inverter, and variable speed drive andefficient selection of the three systems control of the motor. Software and hardware used in thisthesis work are DCS CENTUM VP Yokogawa.

Thomas More’s Correspondence in a Spanish Ambiance

Moreana ◽  
2002 ◽  
Vol 39 (Number 149) (1) ◽  
pp. 41-60 ◽  
Author(s):  
Eugenio M. Olivares Merino
Keyword(s):  
The Subject ◽  
Selection Of

The recent reprinting of Álvaro de Silva’s 1998 edition of a selection of More’s letters prompts the author to examine the subject of Spanish translations of More, and of de Silva’s general commentary on More’s correspondence and on his relationship to other humanists. The author reflects on aspects of More’s personality as exposed in his letters and uses what he finds as a corrective to several biographical misconceptions. He points out the strengths and weaknesses of de Silva’s work and compares it with that of other translators, particularly Elizabeth Rogers, and notes the particularly Spanish quality of de Silva’s edition.

COMBINATORIAL POLYNOMIALLY COMPUTABLE CHARACTERISTICS OF SUBSTITUTIONS AND THEIR PROPERTIES

2020 ◽  
Vol 7 (2) ◽  
pp. 34-41
Author(s):  
VLADIMIR NIKONOV ◽  
◽  
ANTON ZOBOV ◽  
Keyword(s):  
Mathematical Expectation ◽  
Point Of View ◽  
Small Range ◽  
Computational Point ◽  
Wide Range ◽  
Bijective Function ◽  
The Difference ◽  
Element Base ◽  
Selection Of

The construction and selection of a suitable bijective function, that is, substitution, is now becoming an important applied task, particularly for building block encryption systems. Many articles have suggested using different approaches to determining the quality of substitution, but most of them are highly computationally complex. The solution of this problem will significantly expand the range of methods for constructing and analyzing scheme in information protection systems. The purpose of research is to find easily measurable characteristics of substitutions, allowing to evaluate their quality, and also measures of the proximity of a particular substitutions to a random one, or its distance from it. For this purpose, several characteristics were proposed in this work: difference and polynomial, and their mathematical expectation was found, as well as variance for the difference characteristic. This allows us to make a conclusion about its quality by comparing the result of calculating the characteristic for a particular substitution with the calculated mathematical expectation. From a computational point of view, the thesises of the article are of exceptional interest due to the simplicity of the algorithm for quantifying the quality of bijective function substitutions. By its nature, the operation of calculating the difference characteristic carries out a simple summation of integer terms in a fixed and small range. Such an operation, both in the modern and in the prospective element base, is embedded in the logic of a wide range of functional elements, especially when implementing computational actions in the optical range, or on other carriers related to the field of nanotechnology.

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