Successful embryo transfer following artificial insemination of superovulated fallow deer (Dama dama)

1994 ◽  
Vol 6 (2) ◽  
pp. 181 ◽  
Author(s):  
HN Jabbour ◽  
VS Marshall ◽  
CM Argo ◽  
J Hooton ◽  
AS Loudon
Keyword(s):  
Ovarian Response ◽  
Fallow Deer ◽  
Corpora Lutea ◽  
Dama Dama ◽  
Cell Stage ◽  
Embryo Recovery ◽  
Dama Mesopotamica ◽  
Morula Stage ◽  
The Mean ◽  
Follicular Response

Thirty-four European fallow deer (Dama dama dama) were randomly allocated into embryo donor (n = 12) or embryo recipient (n = 22) groups. All does were treated with controlled internal drug release (CIDR) devices for 14 days. Animals in the embryo donor group were further treated with 200 I.U. pregnant mare serum gonadotrophin (PMSG) and 0.5 units ovine follicle-stimulating hormone (FSH). PMSG was administered 72 h before withdrawal of CIDR devices and FSH was given in eight 0.063 unit injections at 12-hourly intervals starting at the time of PMSG administration. All embryo donor animals were inseminated, by laparoscopy in both uterine horns, 36 h after withdrawal of CIDR devices with 25 x 10(6) fresh spermatozoa collected from Mesopotamian fallow deer (Dama dama mesopotamica). Embryos were recovered by laparotomy on Day 3 (n = 6) or Day 6 (n = 6) after withdrawal of CIDR devices and the ovarian response was determined. In total, 22 embryos were transferred into the oviduct (2-4-cell stage, n = 14) or uterine horn (morula stage, n = 8) on Day 3 or Day 6 after withdrawal of CIDR devices respectively. The overall means (+/- s.e.m.) of total follicular response and corpora lutea were 24.2 +/- 3.5 and 14.1 +/- 3.6 respectively. The mean number of large unruptured follicles was higher on Day 6 than on Day 3 (13.5 +/- 2.9 v. 6.7 +/- 1.3, P < 0.05). The overall embryo recovery rate was 45.8%. There was no difference in pregnancy rate following transfer of embryos on either Day 3 or Day 6 (7/14 v. 5/8 respectively).(ABSTRACT TRUNCATED AT 250 WORDS)

Increased production of sheep embryos following superovulation of Merino ewes with a combination of pregnant mare serum gonadotrophin and follicle stimulating hormone

1991 ◽  
Vol 3 (5) ◽  
pp. 551 ◽  
Author(s):  
JP Ryan ◽  
JR Hunton ◽  
WM Maxwell
Keyword(s):  
Follicle Stimulating Hormone ◽  
Ovarian Response ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Embryo Recovery ◽  
Designed Experiment ◽  
The Mean ◽  
Mean Concentration ◽  
Pregnant Mare Serum Gonadotrophin ◽  
Stimulating Hormone

In a factorially designed experiment (N = 321), 0, 800 or 1600 I.U. pregnant mare serum gonadotrophin (PMSG) were administered in combination with 0, 12 or 18 mg follicle stimulating hormone (FSH-P) to superovulate Merino ewes in autumn and spring. A moderate dose of PMSG (800 I.U.) in conjunction with 12 or 18 mg FSH-P increased the ovulation rate above that observed when FSH-P was used alone. This was accomplished by (i) increasing the proportion of ewes that exhibited a superovulatory response (greater than 3 corpora lutea (CL) or persistent large follicles (LF): 69/70 (99%) v. 55/74 (74%), P less than 0.001), and (ii) in those ewes that exhibited a superovulatory response, by an additive effect of exogenous gonadotrophin (14.8 +/- 0.9 CL (69) v. 11.3 +/- 0.9 CL (55), P less than 0.01) without increasing the incidence of LF. The use of 1600 I.U. PMSG in conjunction with 12 or 18 mg FSH-P was characterized by an increase in the number of LF and, in comparison with 800 I.U. PMSG, a reduction in ovulation rate. Season had no effect on the numbers of CL, but total ovarian response (CL + LF) was higher in autumn than in spring (P less than 0.01), because of a greater incidence of LF (P less than 0.001). The proportion of ewes with regressed CL was higher in autumn than in spring (53/143 (37%) v. 32/156 (21%), P less than 0.01), and increased with increased dose of gonadotrophin. Furthermore, a nutritional component to the incidence of ewes with regressed CL was suggested by the observation that the mean concentration of plasma glucose was higher for ewes with normal CL than for ewes with regressed CL (P less than 0.05). Rates of ova or embryo recovery, fertilization and embryo development generally declined with an increase in the incidence of LF as a result of increases in the dose of gonadotrophin and season of administration.

scholarly journals Responses of the Black Bengal goat (Capra hircus) to PMSG and surgical embryo recovery within MOET technique

1970 ◽  
Vol 4 (2) ◽  
pp. 107-115 ◽  
Author(s):  
MO Faruk ◽  
FY Bari ◽  
M Shamsuddin ◽  
MGS Alam ◽  
MF Islam
Keyword(s):  
Dose Rate ◽  
Research Work ◽  
Capra Hircus ◽  
Corpora Lutea ◽  
Embryo Recovery ◽  
The Mean ◽  
One Year ◽  
Natural Service ◽  
Mean Time ◽  
Black Bengal Goat

The aim of the present study was to determine the responses of Black Bengal Goat following synchronization with alfaprostol, superovulation with PMSG and embryo collection with surgical procedure. This research work was carried out at the Department of Surgery and Obstetrics, Bangladesh Agricultural University (BAU) during the period from January to December 2001. Three Flushing schedules have been carried out in one-year time consisting of 5 donor and 4 recipients in Flush I, 2 donors and 4 recipients in Flush II, and 4 donors and 3 recipients in Flush III. The donors as well as the recipients were synchronized with Gabbrostim® (Alfaprostol, VETEM, Italy) at the dose rate of 2-mg equivalent to 1ml/goat. Among 11 donors only 6 donors responded following first injection, but all donor responded with second and third injection of Gabbrostim®. The mean time of onset of oestrus and duration of oestrus in donor were 29.9 ± 9.20h, 50.0 ± 5.94h, 47.8 ± 4.05h and 20.9 ±5.10h, 27.9 ± 1.19h, 29.4 ± 1.36h respectively. Among 11 recipient does, only 6 responded following first injection of Gabbrostim, while that of second injection was 9. The mean time of onset of oestrus and duration of oestrus were 28.6 ± 9.69h, 40.1 ± 8.95h and 17.1 ± 5.05h, 22.5 ± 3.45h respectively. All 9 recipients responded in third injection and the mean time of onset of oestrus and duration of oestrus were 39.9 ± 5.06h and 27.4 ± 1.32h respectively. In all flushing schedules, superovulation was performed using PMSG (Folligon®, Intervet International B.V, Holland) at the dose rate of 900 IU, 800 IU and 700 IU respectively. Both natural service and AI was performed to fertilise the ovum after onset of oestrus. The superovulatory response was determined by counting the number of corpora lutea (CL) during collection of embryos. The percent of superovulatory donor goats were 100, 50 and 25% respectively. The mean number of ovulation was 13.6 ±3.6, 10.5 ± 8.5 and 3.5 ± 3.2 respectively. The embryos were collected surgically by using 50 ml Phosphate buffer saline (PBS). The embryos were counted and graded under stereomicroscope. The embryo collection rates varied from 0-100%. The recovered embryos were of 100% fertile. The total number of embryos recovered was 4, 8 and 8 with the percent of recovery 6, 38 and 61.5% respectively. In Flush I among 4 recovered embryos, 3 were Grade 1 morula and the rest embryo was Grade 2 late morula. In Flush II among 8 recovered embryos, 7 were Grade 2 early morula and rest embryo was Grade 2 late morula. In Flush III among 8 recovered embryos, 5 were Grade 2 morula and rests 3 were Grade 2 late morula. Key words: Black Bengal goat, PMSG, embryo, MOET DOI = 10.3329/bjvm.v4i2.1293 Bangl. J. Vet. Med. (2006). 4 (2): 107-115

Some Remarks on Deer (Cervidae) in Ancient Egypt

1987 ◽  
Vol 73 (1) ◽  
pp. 238-243
Author(s):  
Patrick F. Houlihan
Keyword(s):  
Cervus Elaphus ◽  
Red Deer ◽  
Fallow Deer ◽  
Ancient Egypt ◽  
Dama Dama ◽  
Naturally Occurring ◽  
Dama Mesopotamica ◽  
In The Wild

The evidence for deer (Cervidae) in ancient Egypt is reviewed briefly. The question of whether deer ever existed in the wild as an element of the Egyptian fauna, or were only known from rare exotic imports, cannot be conclusively answered yet. It is quite likely, however, based upon the records of the Egyptians themselves, that deer were native, whether naturally occurring or introduced. While the identification of the species depicted as the Persian Fallow Deer (Dama dama mesopotamica) is probably correct, the Barbary Red Deer (Cervus elaphus) remains a possibility.

scholarly journals Pregnancy rate in indigenous ewes by direct transfer of vitrified embryos

2018 ◽  
Vol 34 (1) ◽  
pp. 27-33 ◽  
Author(s):  
S Ghosh ◽  
MRI Talukder ◽  
PK Jha ◽  
MGS Alam ◽  
NS Juyena ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Standard Procedure ◽  
Prostaglandin F2α ◽  
Corpora Lutea ◽  
Abdominal Ultrasonography ◽  
Mean Values ◽  
Embryo Recovery ◽  
Embryo Yield ◽  
The Mean ◽  
Grade 3

The effects of PMSG on superovulation, quality of embryos, and pregnancy rate were studied following transfer of vitrified embryos into indigenous ewes. Three donor and nine recipient ewes were synchronized using two intramuscular doses of Cloprostenol (PGF2α) equivalent to 125 μg Prostaglandin F2α (Ovoprost® Bayer, New Zealand) at an interval of nine days. To ensure ovulation donor and recipient ewes were treated with 600 iu and 250 iu Pregnant Mare Serum Gonadotrophin (PMSG; Folligon®, Intervet, Boxmeer, The Netherlands) at the time of 2nd injection of Prostaglandin F2α. All donor ewes were mated by fertile rams. Embryos were collected from donor ewes on day six after mating by inguinal laparotomy. Grade 1 embryos were vitrified using standard procedure. After thawing the embryos were transferred into the exteriorized uterine horn of the recipient by inguinal laparotomy. Pregnancy diagnosis was performed by trans-abdominal ultrasonography on Day 40 of transfer. Oestrus occurred 31.3 ± 8.1 hours after second injection of prostaglandin and 32.6 ± 8.7 hours in recipients. Oestrus lasted 24.7 ± 9.9 hours in donors and 32.8 ± 12.8 hours in recipients. The mean numbers of corpora lutea and embryos of donor ewes were 11.7 ± 4.0 and 8 ± 2.6, respectively. Total embryo recovery rate of donor ewes was 68.6%. The mean values of qualities of embryo yield of donor ewes were 6 ± 1.7 grade 1, 0.3 ± 0.6 grade 2, 1.3 ± 1.2 grade 3, and 0.3 ± 0.6 grade 4. Twenty embryos were vitrified, 16 embryos were transferred and four recipient ewes were found pregnant. The pregnancy rate of recipient ewes was 44.4%.Bangl. vet. 2017. Vol. 34, No. 1, 27-33

Immobilization of captive Persian fallow deer (Dama dama mesopotamica) using medetomidine–ketamine or medetomidine–midazolam

2019 ◽  
Vol 46 (5) ◽  
pp. 662-666
Author(s):  
Nili Avni-Magen ◽  
Barak Zafrir ◽  
Roni King ◽  
Tali Bdolah-Abram ◽  
Yael Shilo-Benjamini
Keyword(s):  
Fallow Deer ◽  
Dama Dama ◽  
Dama Mesopotamica

Effects of cloprostenol and flurogestone acetate sponge on embryo yields and quality in indigenous ewes in Bangladesh

2014 ◽  
Vol 54 (10) ◽  
pp. 1605 ◽  
Author(s):  
B. F. Zohara ◽  
Azizunnesa ◽  
M. F. Islam ◽  
M. G. S. Alam ◽  
F. Y. Bari
Keyword(s):  
Recovery Rate ◽  
Group Iv ◽  
Corpora Lutea ◽  
Group Iii ◽  
Embryo Recovery ◽  
Fertilisation Rate ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
Mean Time

The effects of two doses of cloprostenol and two doses of flurogestone acetate sponge on the onset of oestrus, and embryo recovery and quality were evaluated. Thirty-two indigenous ewes (Wera breed) were allocated into four groups of eight. All ewes were synchronised with 100 µg (Group I) or 175 µg (Group II) cloprostenol injection, 9 days apart, or insertion of intravaginal sponges containing 30 mg (Group III) or 45 mg (Group IV) flurogestone acetate (FGA) for 12 days. The ewes were superovulated with 600 IU PMSG intramuscularly 10 days after the second cloprostenol injection or immediately after sponge removal on Day 12. After the detection of oestrus, the ewes were mated naturally at 6 and 12 h, and some ewes were inseminated laparoscopically. Embryos were recovered surgically 5 or 6 days after service. All ewes exhibited oestrus. The onset of oestrus occurred significantly (P < 0.05) earlier in FGA-treated (50.0 ± 1.5 and 48.0 ± 0.00 h) than in cloprostenol-treated groups. There were no significant differences (P > 0.05) in the mean time of onset of oestrus (50.0 ± 1.5 and 48.0 ± 0.00 h) between the two doses of cloprostenol. The mean number of corpora lutea (8.1 ± 1.26) and embryos recovered (6.1 ± 1.00) was significantly (P < 0.05) higher in ewes treated with 45 mg FGA than in ewes treated with cloprostenol. Embryo recovery rate was significantly (P < 0.05) higher in ewes treated with 45 mg FGA (75.4%) than in other groups (41.7% and 51.6% in 100 μg and 175 μg cloprostenol- and 52.7% in 30 mg FGA-treated groups, respectively). Fertilisation rate was 93.3% in ewes given 100 µg cloprostenol, whereas other groups showed 100% fertilisation rate. The highest percentage (100%) of Grade 1 embryos was in FGA groups. In conclusion, despite FGA protocol presenting superior results, cloprostenol protocol was equally efficient in synchronising oestrus. The embryo recovery rate was better after 45 mg FGA than 30 mg FGA or after either dose of cloprostenol.

scholarly journals Selected haematological indices in farmed male fallow deer (Dama dama) depending on the different conditions during the wintering period

2019 ◽  
Vol 64 (No. 9) ◽  
pp. 379-385
Author(s):  
K Tajchman
Keyword(s):  
Nutritional Status ◽  
Protein Content ◽  
Fallow Deer ◽  
Lower Amount ◽  
Physiological Status ◽  
Dama Dama ◽  
Animal Group ◽  
Distribution Width ◽  
The Mean ◽  
The Impact

Fallow deer (Dama dama) are the most common breeding species among farmed cervids in Poland. Monitoring the animals’ health, nutritional status, and welfare are highly important aspects in their breeding. Haematological variables are important indicators for comparing the physiological status of the animals and for monitoring the changes in the organism related to the adaptation to the breeding conditions. The aim of this study was to assess the impact of the day’s length and the total protein content in the diet for the farmed fallow deer on the selected haematological variables. The study demonstrated a significant decline in the mean corpuscular volume (MCV) and an increase of the mean corpuscular haemoglobin concentration (MCHC) (P &lt; 0.05) in all the animals after the winter period. However, the animal group exposed to prolonged daylight exhibited a significant increase in the platelet distribution width (PDW) and platelet large cell ratio (P-LCR) (P &lt; 0.05). In turn, the group receiving a lower amount of protein in the diet was characterised by a significant reduction in the platelet count (PLT) (P &lt; 0.05). Thus, the length of daylight and the protein content of the diet for fallow deer exert a significant impact on several haematological characteristics, which may serve as indicators of an animal’s nutritional status and welfare.

scholarly journals Laparoscopic intrauterine insemination of Awassi ewes superovulated with equine chorionic gonadotropin

2010 ◽  
Vol 9 (2) ◽  
pp. 1
Author(s):  
O. I. Azawi, and M. K. M. A. Al-Mola
Keyword(s):  
Chorionic Gonadotropin ◽  
Intrauterine Insemination ◽  
Ovarian Response ◽  
Fertilization Rate ◽  
Distal Portion ◽  
Laparoscopic Technique ◽  
Corpora Lutea ◽  
Embryo Recovery ◽  
Equine Chorionic Gonadotropin ◽  
Laparoscopic Techniques

The present study was undertaken to investigate the contribution of laparoscopic insemination to the improvement of fertilization and embryo recovery. The experiment was conducted in the breeding season. Twelve nonpregnant and cycling Awassi ewes of 3-4 years of age were randomly allocated in equal number (n = 6) to two groups. Each ewe was treated with progesterone impregnated intravaginal sponge for 12 days. All ewes were superovulated with eCG (equine chorionic gonadotropin) 1200 IU once by intramuscular injection 48 h prior to sponge removal. Ewes of group 1 were mated naturally at least two times with Awassi rams of proven fertility. Ewes of group 2 had intrauterine insemination and were conducted 44-46 h after sponge removal, under laparoscopic visualization of uterine horns, depositing 1 ml of semen in the distal portion of each uterine horn. Ovarian response was assessed by determining the number of corpora lutea by laparoscopy on day 6 after mating. Embryo recovery was performed by semi-laparoscopic and by flushing of both uterine horns. Results of the present study revealed a high number of unfertilized ova (P<0.05) was observed in ewes when naturally inseminated than ewes inseminated intrauterine using the laparoscopic technique. The higher rate of embryo recovery (P<0.05) was achieved when ewes inseminated via intrauterine (4.66 ± 0.66) than ewes naturally mated (2.16 ± 0.74). The fertilization rate in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 79.93% and 40.07%, respectively. Fertilization failure in ewes inseminated intrauterine using laparoscopic techniques and naturally mated were 7.37% and 49.35%, respectively. It could be concluded that the use of eCG to induce superovulation in Awassi ewes combined with laparoscopic intrauterine insemination can increase the fertilization rate.

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