Exogenous progesterone and embryo survival in Booroola-cross ewes

1991 ◽  
Vol 3 (1) ◽  
pp. 71 ◽  
Author(s):  
DO Kleemann ◽  
SK Walker ◽  
RJ Grimson ◽  
DH Smith ◽  
TI Grosser ◽  
...  

To investigate if exogenous progesterone improves embryo survival, 209 multiparous Booroola Merino x South Australian Merino ewes, heterozygous for the F gene (F+) were allocated to seven treatment groups and inseminated at a synchronized oestrus. Six groups received progesterone from controlled internal drug release G dispensers on the following days after ovulation: 4-7, 4-11, 4-14, 7-11, 7-14 and 11-14. Concentration of peripheral progesterone increased (P less than 0.05) in most supplemented groups, but there were no significant differences in pregnancy rates between treatments. However, the number of fetuses per pregnancy was increased for progesterone treatments starting on Day 4 (Days 4-7, 4-11 and 4-14 combined v. control; P less than 0.05) and for all supplemented treatments compared with the control (P less than 0.05).

2013 ◽  
Vol 25 (1) ◽  
pp. 153 ◽  
Author(s):  
H. Richard ◽  
P. Appleton ◽  
D. J. Kesler ◽  
C. E. Ferguson

The purpose of this study was to determine the effectiveness of increasing progesterone (P4) concentrations during early pregnancy, by supplementing a low dose of Regu-Mate® (Intervet, Millsboro, DE, USA) on Days 3, 4, and 5, post-ovulation to AI mares. Sixty-three AI-bred mares were randomly allocated to 1 of 2 treatment groups. All mares underwent ovulation induction using 1.5 mg mL–1 of deslorelin acetate (IM) following observation of ≥30-mm follicles and were inseminated with either fresh (n = 57) or cooled (n = 6) semen from fertile stallions approximately 24 h later. Ovulation was verified via ultrasonography in all mares. Mares allocated to the treatment group (n = 29) received altrenogest (Regu-Mate®) orally at a rate of 15 mg per day on Days 3, 4, and 5 following ovulation (d = 0), whereas control mares (n = 34) received no treatment. Blood samples were collected from all mares from Day 0 (ovulation) through Day 6 post-ovulation via jugular venipuncture. Pregnancies were determined via ultrasonography at 18 days post-ovulation. The pregnancy rate for untreated mares (18/34, 53%) was not different (P > 0.05) from altrenogest-treated mares (13/29, 45%). There was no difference (P > 0.50) in pregnancy rates when mares were AI bred 1 (19/39, 49%) or 2 (10/20, 50%) days from ovulation. The mean plasma P4 concentration on Day 5 was higher (P < 0.06) for pregnant control mares (4.75 ± 0.59 ng mL–1) compared with open control mares (3.50 ± 0.25 ng mL–1). There was a greater increase (P < 0.02) in plasma P4 concentrations (Day 5 – Day 1 concentration) in untreated pregnant mares (3.87 ± 0.56 ng mL–1) compared with untreated open mares (2.38 ± 0.22 ng mL–1). In conclusion, it was determined that altrenogest supplementation from Day 3 to 5 post-ovulation was ineffective in improving pregnancy rates. However, it is unknown if this was the result of the dose used or the length of supplementation. Additionally, the timing of altrenogest coincided with a significant increase in P4 in pregnant mares compared with open mares, indicating that the timing may be correct or there was action of an unknown embryonic luteotropin(s), which would describe the differences between P4 concentrations in pregnant versus open mares.


2020 ◽  
Vol 7 (2) ◽  
pp. 156
Author(s):  
Silvia Rani Andriyanti ◽  
Mas’ud Hariadi ◽  
Roesno Darsono ◽  
Pudji Srianto ◽  
Wurlina Wurlina ◽  
...  

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a  73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.


2004 ◽  
Vol 16 (2) ◽  
pp. 182 ◽  
Author(s):  
R. Steel ◽  
J.F. Hasler

Although it has not been documented in published studies, embryo transfer (ET) practitioners have suggested that embryos from Jersey (JE) cattle do not survive freezing as well as embryos from other dairy breeds such as Holsteins (HO). The present study represents a retrospective analysis of pregnancy rates achieved following transfer of fresh and frozen embryos from Jersey and Holstein donors. In addition, a retrospective comparison was made of two different embryo-freezing protocols for each breed of cattle. Embryos were collected nonsurgically 7 to 7.5 days post-estrus from superovulated donors on 57 Holstein and 27 Jersey dairy farms over a 15-year period. Fresh and frozen-thawed embryos were transferred nonsurgically into cows and heifers following either natural or prostaglandin-induced estrus. Embryos were frozen either in 10% glycerol (Gly) or 1.5M ethylene glycol (EG) in 0.25mL straws. Following equilibration, straws were seeded at −6 to −7°C and temperature was maintained for 10 min and then decreased at 0.6°Cper min. Straws were plunged into liquid nitrogen at −32 to −35°C. At thawing, straws were held in the air for 7s and then submerged in 29°C water for 15s. Embryos frozen in EG were transferred immediately following thawing. Embryos frozen in Gly were rehydrated in a standard 3-step Gly-sucrose system prior to being transferred. Pregnancy diagnosis was performed at Days 40 to 90 of gestation. As seen in the Table 1, pregnancy rates were similar for fresh embryos from both HO and JE cattle. Also, there were no differences in pregnancy rates between recipients that received embryos frozen in Gly or EG within donors of either breed. However, JE embryos frozen in either Gly or EG resulted in lower pregnancy rates than did HO embryos frozen in Gly or EG. Embryo stage at freezing was tracked for EG but not Gly embryos. There were no differences in pregnancy rates among morulae, early blastocysts or mid-blastocysts for either HO or JE embryos frozen in EG. The differences in embryo survival may be due to different lipid composition of embryos of the two breeds. Perhaps a more efficacious freezing protocol can be developed for cryopreservation of JE embryos. In conclusion, pregnancy rates with cryopreserved HO embryos were higher than with JE embryos. Table 1


Author(s):  
Stephanie J. Ventura ◽  
William D. Mosher ◽  
Sally C. Curtin ◽  
Joyce C. Abma ◽  
Stanley Henshaw

2018 ◽  
Vol 52 (1-4) ◽  
pp. 22-30
Author(s):  
A K Paul ◽  
S M T Rahman

Hormonal treatment of cows at the coastal region of Barisal district of Bangladesh was performed to assess the improvement of pregnancy rate. A total of 100 cows and heifers with irregular history of cyclicity were selected randomly and divided into five treatment groups. The groups were A (treated with anthelmintic), B (treated with anthelmintic, vitamin ADE and multivitamin powder), C (treated with PGF2α), D (treated with GnRH) and E (treated with GnRH and PGF2α). Each group comprised of 20 animals. The age, breed and parity of experimental cows were considered during treatment. In the study, the cows treated with both GnRH and PGF2α (group E) showed significantly (p<0.05) higher estrus (80%) and pregnancy rate (60%) than that of group A, B, C and D. The overall estrus rates of local and crossbred cows were 64% and 70%, respectively and the pregnancy rates were 40 and 52%, respectively. The crossbred cows responded significantly (p<0.05) to hormonal treatment than that of local cows. Parity-2 cows showed higher estrus sign than that of other parities. However, the pregnancy rates were higher significantly (p<0.05) in parity-2 and parity ≥4 cows than that of parity-0, parity-1 and parity-3 cows. The pregnancy rate was also found higher in case of 4 to <5 years old cows than that of 2 to <3, 3 to <4, 5 to <6, and ≥6 years old. It may conclude that the hormonal regimen increases the pregnancy rate as well as decreases the undesired waiting of estrus and conception. Further study with more sample size will reveal the more effective treatment for cows at the coastal areas of Bangladesh.


2018 ◽  
Vol 52 (1-4) ◽  
pp. 22-30
Author(s):  
A K Paul ◽  
S M T Rahman

Hormonal treatment of cows at the coastal region of Barisal district of Bangladesh was performed to assess the improvement of pregnancy rate. A total of 100 cows and heifers with irregular history of cyclicity were selected randomly and divided into five treatment groups. The groups were A (treated with anthelmintic), B (treated with anthelmintic, vitamin ADE and multivitamin powder), C (treated with PGF2α), D (treated with GnRH) and E (treated with GnRH and PGF2α). Each group comprised of 20 animals. The age, breed and parity of experimental cows were considered during treatment. In the study, the cows treated with both GnRH and PGF2α (group E) showed significantly (p<0.05) higher estrus (80%) and pregnancy rate (60%) than that of group A, B, C and D. The overall estrus rates of local and crossbred cows were 64% and 70%, respectively and the pregnancy rates were 40 and 52%, respectively. The crossbred cows responded significantly (p<0.05) to hormonal treatment than that of local cows. Parity-2 cows showed higher estrus sign than that of other parities. However, the pregnancy rates were higher significantly (p<0.05) in parity-2 and parity ≥4 cows than that of parity-0, parity-1 and parity-3 cows. The pregnancy rate was also found higher in case of 4 to <5 years old cows than that of 2 to <3, 3 to <4, 5 to <6, and ≥6 years old. It may conclude that the hormonal regimen increases the pregnancy rate as well as decreases the undesired waiting of estrus and conception. Further study with more sample size will reveal the more effective treatment for cows at the coastal areas of Bangladesh.


1968 ◽  
Vol 71 (2) ◽  
pp. 215-222 ◽  
Author(s):  
F. W. H. Elsley ◽  
R. M. MacPherson ◽  
I. McDonald

SUMMARYFifty-two Large White gilts, arranged in thirteen groups of four litter sisters, were allocated at mating to one of four treatment groups. These treatments, arranged as a 2 × 2 factorial, allowed daily energy intakes in pregnancy of 8·3 Meal (H(e)) or 5·2 Meal (L(e)) and of average daily intake during lactation of 20 Meal (h(e)) or 13·8 Meal (m(e)). Daily intakes of protein, minerals and vitamins were similar for all treatments. There were no consistent differences in health or breeding regularity between the treatments.The H(e) sows had a net gain in pregnancy 22 kg greater than the L(e) sows. Feeding the higher level in lactation (h(e) resulted in a reduced sow weight loss during lactation of 20, 12 and 10 kg in the first, second and third parities respectively, as compared with the m(e) sows. There were no consistent effects on the numbers of pigs born apart from the first litter in which L(e) sows produced significantly larger litters at birth. The higher energy intakes in pregnancy and lactation led to very similar increases in the weights of the piglets, of the order of 0.1 kg at birth, 0.4 kg at 3 weeks of age, and 1.1 kg at 8 weeks of age. Sowsgiven the same overall energy intake throughout their reproductive life (H(e) m(e), L(e) h(e)) at the end of the third litter had similar live weights and had produced a similar total weight of weaned pigs. The practical implications of these results are discussed.


Author(s):  
Abubakr O. Ismail ◽  
◽  
Erneo B. Ochi ◽  
Ambrose S. Jubara ◽  
Bakri Ahmed A/Rahim ◽  
...  

A comparative hormonal study of prostaglandin, gonadotropin releasing hormone and their combination was conducted on 30 randomly selected cross-bred dairy cows of SEMEX project for artificial insemination in Hillat Kuku, Khartoum North, Sudan. The study attempts to determine the response of the animals to hormonal induction and synchronization of estrous as well as fertility following fixed time artificial insemination. Three treatment groups of 10 cows each were undertaken. Groups 1, 2 and 3 involved administration of prostaglandin F2α (PGF2α), gonadotropin releasing hormone (GnRH) and a combination of PGF2α + GnRH, respectively. Descriptive statistical analysis was performed. The results revealed that all the three protocols induced and synchronized estrous were almost synchronous in all the treated groups. However, protocol 3 revealed a significantly (p<0.05) better result of 70% pregnancy rate compared to other two protocols that equally provided 50% pregnancy rates. The study resolves that protocol 3 be highly recommended to change the mindset of farmers about the spread of reproductive technology in Sudan. Protocols 1 and 2 provided acceptable pregnancy rates that can be enhanced with the improvement of management.


Author(s):  
I KOMANG EVAN WIJAKSANA ◽  
Lambang Bargowo ◽  
Shafira Kurnia Supandi

During the Covid-19 pandemic, there was an increase in pregnancy rates in East Java. Besides efforts to suppress pregnancy rates, it is also necessary to ensure the health of the mother and fetus in order to be born healthy. The prevalence of periodontal diseases in pregnant women is high, while the dental visit of pregnant women is low. Periodontal disease is one of the risk factors of preterm birth as well as low-birth-weight (LBW) infants. In this community service, socialization held through lecture methods, QnA, discussion and self-practice procedures to maintain oral health procedures. The goal is to improve pregnant women's knowledge of the importance of dental and oral care, especially periodontal tissue during pregnancy and enhance skills of pregnant women in detecting early symptoms of periodontal diseases and to perform oral dental care independently at home. The knowledge and skills of pregnant women can be improved during this community service. By healthy periodontal tissue and teeth, it is expected that the risk of LBW infants and also preterm birth can be decreased.abstrakSelama masa pandemi Covid-19, terjadi peningkatan tingkat kehamilan di Jawa Timur. Selain usaha menekan tingkat kehamilan, perlu juga dipastikan kesehatan ibu dan janin agar lahir dengan sehat. Prevalensi kelainan periodontal pada ibu hamil tinggi sementara kunjungan ibu hamil ke dokter gigi rendah. Penyakit periodontal merupakan salah satu faktor resiko bayi lahir prematur dan juga berat badan lahir rendah (BBLR). Dalam pengabdian masyarakat ini dilakukan sosialisasi melalui metode ceramah, tanya jawab, diskusi dan praktek mandiri prosedur menjaga kesehatan rongga mulut. Tujuannya adalah meningkatkan pengetahuan ibu hamil mengenai pentingnya perawatan gigi dan mulut khususnya jaringan periodontal selama kehamilan dan meningkatnya ketrampilan ibu hamil dalam mendeteksi gejala awal kelainan periodontal dan cara melakukan perawatan gigi mulut mandiri di rumah. Pada pengabdian masyarakat ini pengetahuan dan ketrampilan ibu hamil dapat ditingkatkan. dengan jaringan periodontal dan gigi serta rongga mulut yang sehat, diharapkan resiko BBLR maupun kelahiran prematur dapat diturunkan.


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