Influence of postnatal prolactin modulation on the development and maturation of ventral prostate in young rats

2018 ◽  
Vol 30 (7) ◽  
pp. 969
Author(s):  
Ana C. L. Camargo ◽  
Flávia B. Constantino ◽  
Sérgio A. A. Santos ◽  
Ketlin T. Colombelli ◽  
Maeli Dal-Pai-Silva ◽  
...  
Keyword(s):  
Serum Testosterone ◽  
Receptor Expression ◽  
Ventral Prostate ◽  
Sprague Dawley ◽  
Differentiated Cells ◽  
Cell Height ◽  
Lack Of Information ◽  
Rat Ventral Prostate ◽  
Sprague Dawley Rats ◽  
Acinar Lumen

Besides androgenic dependence, other hormones also influence the prostate biology. Prolactin has been described as an important hormone associated with maintenance of prostatic morphophysiology; however, there is a lack of information on the involvement of prolactin during prostate development and growth. This study aimed to evaluate whether perinatal prolactin modulation interferes with rat ventral prostate (VP) development and maturation. Therefore, prolactin or bromocriptine (an inhibitor of prolactin release from the pituitary) were administered to Sprague Dawley rats from postnatal Day (PND) 12 to PND 21 or 35. Animals were then killed and serum hormonal quantification, VP morphological–stereological and immunohistochemical analyses and western blotting reactions were employed. Our results demonstrate that prolactin blockage increased serum testosterone on PND 21, which reflected an increase in anogenital distance. Although prolactin modulation did not interfere with VP weight, it modified VP morphology by dilating the acinar lumen and reducing epithelial cell height. Prolactin activated the signal transducer and activator of transcription (STAT) downstream pathway, increased androgen receptor expression and epithelial proliferation. In addition, prolactin and bromocriptine also increased expression of cytokeratin 18, a marker of luminal-differentiated cells. In conclusion, the VP responds to prolactin modulation through a mechanism of increasing the epithelial proliferative response and dynamics of cell differentiation, especially in animals treated for a more prolonged period.

scholarly journals NIFEDIPINEON;

2019 ◽  
Vol 26 (02) ◽  
Author(s):  
Sidra Hamid ◽  
Qaiser Aziz ◽  
Aneela Jamil ◽  
Lubna Meraj ◽  
Shazia Muazam ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Luteinizing Hormone ◽  
Serum Testosterone ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Channel Blockers ◽  
Sprague Dawley ◽  
Study Objective ◽  
Serum Luteinizing Hormone ◽  
Sprague Dawley Rats

Background: The most potent and effective drugs used for the management of blood pressure in hypertensive patients are Calcium channel blockers (CCBs). Nifedipine, a CCB, acts by blocking entry of calcium ions all the way through the voltage gated calcium channels (VGCCs) of L-type present in the smooth muscle cells of blood vesselsand reducing the blood pressure by decreasing the peripheral vascular resistance. Objectives: The study objective was to determine the effect of nifedipine on serum luteinizing hormone (LH) and serum testosterone in male Sprague Dawley rats. Study Design: Animal experimental study. Setting: All experiments were conducted at the Research laboratory of Shifa College of Medicine, Islamabad along with National Institute of Health (NIH), Islamabad. Period: October, 2012 to April, 2014. Methods: The study was done on adult male Sprague-Dawley rats (N= 60) aged 90-120 days old and their body weights varied between 200 + 50 grams. Rats were divided intotwo groups (n=30). Group A was administered0.5 ml distilled water/rat daily orally, group B was administered orally with nifedipine 50 mg/kg/rat dissolved in 1ml of DMSO. All the doses were given to rats for 8 weeks. After 8 weeks, serum luteinizing hormone and serum testosterone were measured in both groups. Results: In Nifedipine treated group, serum testosterone was significantly decreasedand serum LH was unaffected as compared to the control group. Conclusion: Nifedipine has adverse effects on male fertility as it decreases serum testosterone level.

Chronic fructose intake does not induce liver steatosis and inflammation in female Sprague–Dawley rats, but causes hypertriglyceridemia related to decreased VLDL receptor expression

2018 ◽  
Vol 58 (3) ◽  
pp. 1283-1297 ◽  
Author(s):  
Gemma Sangüesa ◽  
José Carlos Montañés ◽  
Miguel Baena ◽  
Rosa María Sánchez ◽  
Núria Roglans ◽  
...  
Keyword(s):  
Liver Steatosis ◽  
Receptor Expression ◽  
Sprague Dawley ◽  
Vldl Receptor ◽  
Fructose Intake ◽  
Sprague Dawley Rats

Testosterone, dihydrotestosterone and androstanediols in plasma, testes and prostates of rats during development

1981 ◽  
Vol 96 (1) ◽  
pp. 127-135 ◽  
Author(s):  
Colette Corpéchot ◽  
Etienne-Emile Baulieu ◽  
Paul Robel
Keyword(s):  
Plasma Concentration ◽  
Sharp Peak ◽  
Ventral Prostate ◽  
Sprague Dawley ◽  
Adult Level ◽  
Broad Maximum ◽  
Young Rats ◽  
Sprague Dawley Rats ◽  
Radio Immunoassay ◽  
Specific Radio

Abstract. Testosterone (T). 5α-dihydrotestosterone (DHT), 5α-androstane-3α, 17β-diol (3α-diol), and 5α-androstane-3β,17β-diol (3β-diol) have been measured in the plasma, ventral prostate, and testes of Sprague-Dawley rats with a specific radio-immunoassay, at birth, and then weekly until the 12th week. In plasma, the T concentration is ∼ 0.3 ng/ml at birth and up to the 4th week; thereafter it increases steadily and reaches the adult level of ∼ 3 ng/ml by the 8th week. In male foetuses of 18 days gestation, the plasma concentration is 0.6 ng/ml. The 5α-metabolites taken together are at a higher concentration than that of T until the 7th week, and each one, after a broad maximum between 5–9 weeks, decreases promptly by the 10th week. The larger formation of 5α-metabolites from T in young rats has been confirmed by injection of the hormone to castrated males, which showed relatively more conversion at 5 than at 10 weeks. In the ventral prostate, DHT is already high at 4 weeks (11 ng/g), and remain between 7 and 15 ng/g until the 12th week. T concentration is also larger than in plasma at 4th week (2 ng/g) and becomes approximately equal to the plasma value at puberty. A sharp peak of 3α-diol and 3β-diol is observed at the 8th week. In the testes, T concentration is high at birth (183 ng/g), then declines rapidly, and increases again from the 5th week onward in parallel with plasma concentration. The concentrations of 5α-metabolites per testis vary in parallel to those in plasma, with the exception of sustained elevation of 3β-diol.

scholarly journals Inhalation of welding fumes reduced sperm counts and high fat diet reduced testosterone levels; differential effects in Sprague Dawley and Brown Norway rats

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Astrid Skovmand ◽  
Aaron Erdely ◽  
James M. Antonini ◽  
Timothy R. Nurkiewicz ◽  
Mohammad Shoeb ◽  
...  
Keyword(s):  
Systemic Inflammation ◽  
High Fat Diet ◽  
Serum Testosterone ◽  
Brown Norway ◽  
Welding Fumes ◽  
Sperm Production ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Norway Rats

Abstract Background Previous studies have shown that inhalation of welding fumes may induce pulmonary and systemic inflammation and organ accumulation of metal, to which spermatogenesis and endocrine function may be sensitive. Also obesity may induce low-grade systemic inflammation. This study aimed to investigate the effects on sperm production of inhaled metal nanoparticles from stainless steel welding, and the potential exacerbation by intake of a high fat diet. Both the inbred Brown Norway and the outbred Sprague Dawley rat strains were included to study the influence of strain on the detection of toxicity. Rats were fed regular or high fat (HF) diet for 24 weeks and were exposed to 20 mg/m3 of gas metal arc-stainless steel (GMA-SS) welding fumes or filtered air for 3 h/day, 4 days/week for 5 weeks, during weeks 7–12. Outcomes were assessed upon termination of exposure (week 12) and after recovery (week 24). Results At week 12, the GMA-SS exposure induced pulmonary inflammation in both strains, without consistent changes in markers of systemic inflammation (CRP, MCP-1, IL-6 and TNFα). GMA-SS exposure lowered daily sperm production compared to air controls in Sprague Dawley rats, but only in GMA-SS Brown Norway rats also fed the HF diet. Overall, HF diet rats had lower serum testosterone levels compared to rats on regular diet. Metal content in the testes was assessed in a limited number of samples in Brown Norway rats, but no increase was obsedrved. At week 24, bronchoalveolar lavage cell counts had returned to background levels for GMA-SS exposed Sprague Dawley rats but remained elevated in Brown Norway rats. GMA-SS did not affect daily sperm production statistically significantly at this time point, but testicular weights were lowered in GMA-SS Sprague Dawley rats. Serum testosterone remained lowered in Sprague Dawley rats fed the HF diet. Conclusion Exposure to GMA-SS welding fumes lowered sperm production in two strains of rats, whereas high fat diet lowered serum testosterone. The effect on sperm counts was likely not mediated by inflammation or lowered testosterone levels. The studied reproductive outcomes seemed more prone to disruption in the Sprague Dawley compared to the Brown Norway strain.

scholarly journals Glucocorticoids act in the dorsal hindbrain to modulate baroreflex control of heart rate

2006 ◽  
Vol 290 (4) ◽  
pp. R1003-R1011 ◽  
Author(s):  
Andrea G. Bechtold ◽  
Deborah A. Scheuer
Keyword(s):  
Heart Rate ◽  
Arterial Pressure ◽  
Logistic Function ◽  
Receptor Expression ◽  
Arterial Baroreflex ◽  
Sprague Dawley ◽  
Baroreflex Control ◽  
Sprague Dawley Rats ◽  
Corticosteroid Receptor ◽  
Dorsal Hindbrain

Systemic corticosterone (Cort) modulates arterial baroreflex control of both heart rate and renal sympathetic nerve activity. Because baroreceptor afferents terminate in the dorsal hindbrain (DHB), an area with dense corticosteroid receptor expression, we tested the hypothesis that prolonged activation of DHB Cort receptors increases the midpoint and reduces the gain of arterial baroreflex control of heart rate in conscious rats. Small (3–4 mg) pellets of Cort (DHB Cort) or Silastic (DHB Sham) were placed on the surface of the DHB, or Cort was administered systemically by placing a Cort pellet on the surface of the dura (Dura Cort). Baroreflex control of heart rate was determined in conscious male Sprague Dawley rats on each of 4 days after initiation of treatment. Plots of arterial pressure vs. heart rate were analyzed using a four-parameter logistic function. After 3 days of treatment, the arterial pressure midpoint for baroreflex control of heart rate was increased in DHB Cort rats (123 ± 2 mmHg) relative to both DHB Sham (108 ± 3 mmHg) and Dura Cort rats (109 ± 2 mmHg, P < 0.05). On day 4, baseline arterial pressure was greater in DHB Cort (112 ± 2 mmHg) compared with DHB Sham (105 ± 2 mmHg) and Dura Cort animals (106 ± 2 mmHg, P < 0.05), and the arterial pressure midpoint was significantly greater than mean arterial pressure in the DHB Cort group only. Also on day 4, maximum baroreflex gain was reduced in DHB Cort (2.72 ± 0.12 beats·min−1·mmHg−1) relative to DHB Sham and Dura Cort rats (3.51 ± 0.28 and 3.37 ± 0.27 beats·min−1·mmHg−1, P < 0.05). We conclude that Cort acts in the DHB to increase the midpoint and reduce the gain of the heart rate baroreflex function.

scholarly journals Potential of Moringa oleifera in the Treatment of Benign Prostate Hyperplasia: Role of Antioxidant Defence Systems

2017 ◽  
Vol 27 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Ismail O. Ishola ◽  
Kayode O. Yemitan ◽  
Olasunmbo O. Afolayan ◽  
Charles C. Anunobi ◽  
Tobi E. Durojaiye
Keyword(s):  
Prostate Specific Antigen ◽  
Moringa Oleifera ◽  
Specific Antigen ◽  
Serum Testosterone ◽  
Peak Effect ◽  
Sprague Dawley ◽  
Antioxidant Defence ◽  
Prostate Hyperplasia ◽  
Sprague Dawley Rats

Objective: This study sought to evaluate the protective effect of ethanolic leaf extract of Moringa oleifera on testosterone-induced benign prostatic hyperplasia (BPH) in male Sprague-Dawley rats. Materials and Methods: BPH was induced in rats by the administration of testosterone propionate (3 mg/kg, s.c., in olive oil) for 4 weeks. M. oleifera (50, 100, or 200 mg/kg), celecoxib (20 mg/kg), or M. oleifera (50 mg/kg) + celecoxib (20 mg/kg) were orally administered daily 15 min before testosterone. On day 29, blood was collected to measure the levels of serum testosterone and prostate-specific antigen before the animals were sacrificed. The prostates were weighed, assayed, and histologically examined. Results: M. oleifera significantly reduced the testosterone-induced increase in prostate weight (20.16%), prostate index (65.85%), serum testosterone (72.86%), and prostate-specific antigen (48.49%). Testosterone caused a significant increase in malondialdehyde (73%) as well as a reduction in glutathione (62.5%), superoxide dismutase (50%), and catalase (64%) activities which were attenuated by M. oleifera with a peak effect obtained at 100 mg/kg. The disruption of prostate histoarchitecture by testosterone was also ameliorated by M. oleifera. Conclusion: M. oleifera prevented testosterone-induced BPH through enhancement of antioxidant defence mechanisms, and hence could be used as an adjunct in the treatment of BPH.

SERUM 5α-ANDROSTANE-3α,17β-DIOL, ANDROSTERONE AND TESTOSTERONE CONCENTRATIONS IN THE IMMATURE MALE RAT: INFLUENCE OF TIME OF DAY

1977 ◽  
Vol 75 (1) ◽  
pp. 177-178 ◽  
Author(s):  
W. H. MOGER ◽  
P. R. MURPHY
Keyword(s):  
Serum Testosterone ◽  
Temporal Variations ◽  
Time Of Day ◽  
Male Rats ◽  
Male Rat ◽  
Sprague Dawley ◽  
Circadian Variations ◽  
Adult Rat ◽  
Immature Male ◽  
Sprague Dawley Rats

Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada, B3H 4H7 (Received 22 April 1977) The concentration of testosterone in the serum of the adult rat varies significantly over a 24 h period (Kinson & Lui, 1973; Howland, 1975; Wilson, McMillan, Seal & Ahmed, 1976). However, studies on circadian variations in serum testosterone concentrations in immature male rats have yielded conflicting results. Grotjan & Johnson (1976) reported significant changes with time in 25–26-day-old Holtzman rats, whereas Döhler & Wuttke (1976) did not observe significant changes in 13–18 or 25–30-day-old Sprague–Dawley rats maintained on the same lighting schedule (14 h light: 10 h darkness). Recently we reported that from 20–35 days of age, 5α-androstane-3α,17β-diol (androstanediol) is the predominant androgen in the circulation of male rats (Moger, 1977). This study was undertaken to determine temporal variations in the concentrations of androstanediol, androsterone and testosterone. Forty-nine male Sprague

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