Production of inbred offspring by intracytoplasmic sperm injection of oocytes from juvenile female mice

2018 ◽  
Vol 30 (3) ◽  
pp. 451 ◽  
Author(s):  
Jie Zhu ◽  
Wei Cui ◽  
Yan-Feng Dai
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Inbred Mice ◽  
Inbred Mouse ◽  
Mouse Strains ◽  
Control Group ◽  
Juvenile Female ◽  
Female Mice ◽  
Adult Mice ◽  
Significant Difference ◽  
Pronuclear Formation

The aim of the present study was to determine whether the use of oocytes from juvenile female mice would improve the efficiency of intracytoplasmic sperm injection (ICSI). In the present study, 15 adult and 14 juvenile C57BL6/J female mice were superovulated, with 17.8 oocytes per mouse harvested from adults, significantly lower than the 40.2 harvested from juveniles (P < 0.01). Sixty and 233 oocytes were harvested from C57BL/6J adult and juvenile mice respectively, activated in 10 mM SrCl2 + 5 μg mL−1 cytochalasin B for 5–6 h and cultured in potassium simplex optimisation medium (KSOM) for 3.5 days, with no differences in morula and blastocyst rates between groups (91.7% vs 96.6%; P > 0.05). Twelve hours after injection of human chorionic gonadotrophin, oocytes were harvested from C57BL/6J juvenile mice into KSOM, randomly divided into groups and activated with the same method mentioned above at 0, 2, 4 or 6 h and then cultured in KSOM for 3.5 days. There was no significant difference in morula and blastocyst rates among the different groups (P > 0.05). Oocytes from juvenile mice activated in 10 mM SrCl2 for 2 h were subjected to ICSI and the rates of pronuclear formation and Day 1 cleavage were significantly improved compared with the control group (P < 0.01). ICSI combined with activation of oocytes from inbred mouse strains (C57BL/6J, C57BL/6N and 129Svev) successfully produced pups. The fertility of some these mice resulting from ICSI was tested, and the animals proved fertile. In conclusion, superovulated juvenile mice can yield more useable oocytes than adult mice, but additional activation is essential for full development of ICSI oocytes harvested from juvenile inbred mice.

scholarly journals Terc Gene Cluster Variants Predict Liver Telomere Length in Mice

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2623
Author(s):  
Dana Zeid ◽  
Sean Mooney-Leber ◽  
Laurel R. Seemiller ◽  
Lisa R. Goldberg ◽  
Thomas J. Gould
Keyword(s):  
Linkage Disequilibrium ◽  
Telomere Length ◽  
Gene Cluster ◽  
Inbred Mice ◽  
Inbred Mouse ◽  
Mouse Strains ◽  
Chromosome 3 ◽  
Human Populations ◽  
Nucleotide Polymorphisms ◽  
Initial Finding

Variants in a gene cluster upstream-adjacent to TERC on human chromosome 3, which includes genes APRM, LRRC31, LRRC34 and MYNN, have been associated with telomere length in several human populations. Currently, the mechanism by which variants in the TERC gene cluster influence telomere length in humans is unknown. Given the proximity between the TERC gene cluster and TERC (~0.05 Mb) in humans, it is speculated that cluster variants are in linkage disequilibrium with a TERC causal variant. In mice, the Terc gene/Terc gene cluster are also located on chromosome 3; however, the Terc gene cluster is located distantly downstream of Terc (~60 Mb). Here, we initially aim to investigate the interactions between genotype and nicotine exposure on absolute liver telomere length (aTL) in a panel of eight inbred mouse strains. Although we found no significant impact of nicotine on liver aTL, this first experiment identified candidate single nucleotide polymorphisms (SNPs) in the murine Terc gene cluster (within genes Lrrc31, Lrriq4 and Mynn) co-varying with aTL in our panel. In a second experiment, we tested the association of these Terc gene cluster variants with liver aTL in an independent panel of eight inbred mice selected based on candidate SNP genotype. This supported our initial finding that Terc gene cluster polymorphisms impact aTL in mice, consistent with data in human populations. This provides support for mice as a model for telomere dynamics, especially for studying mechanisms underlying the association between Terc cluster variants and telomere length. Finally, these data suggest that mechanisms independent of linkage disequilibrium between the Terc/TERC gene cluster and the Terc/TERC gene mediate the cluster’s regulation of telomere length.

scholarly journals Role of Genetic Resistance in Invasive Pneumococcal Infection: Identification and Study of Susceptibility and Resistance in Inbred Mouse Strains

2001 ◽  
Vol 69 (1) ◽  
pp. 426-434 ◽  
Author(s):  
Neill A. Gingles ◽  
Janet E. Alexander ◽  
Aras Kadioglu ◽  
Peter W. Andrew ◽  
Alison Kerr ◽  
...  
Keyword(s):  
Inbred Mice ◽  
Inbred Mouse ◽  
Genetic Resistance ◽  
Pneumococcal Infection ◽  
Mouse Strains ◽  
Inflammatory Lesions ◽  
Invasive Pneumococcal Infection ◽  
Susceptibility And Resistance

ABSTRACT From a panel of nine inbred mice strains intranasally infected withStreptococcus pneumoniae type 2 strain, BALB/c mice were resistant and CBA/Ca and SJL mice were susceptible to infection. Further investigation revealed that BALB/c mice were able to prevent proliferation of pneumococci in the lungs and blood, whereas CBA/Ca mice showed no bacterial clearance. Rapidly increasing numbers of bacteria in the blood was a feature of CBA/Ca but not BALB/c mice. In the lungs, BALB/c mice recruited significantly more neutrophils than CBA/Ca mice at 12 and 24 h postinfection. Inflammatory lesions in BALB/c mice were visible much earlier than in CBA/Ca mice, and there was a greater cellular infiltration into the lung tissue of BALB/c mice at the earlier time points. Our data suggest that resistance or susceptibility to intranasal pneumococci may have an association with recruitment and/or function of neutrophils.

scholarly journals Ketamine exposure in adult mice leads to increased cell death in C3H, DBA2 and FVB inbred mouse strains

2008 ◽  
Vol 92 (1-3) ◽  
pp. 217-227 ◽  
Author(s):  
Chalon R. Majewski-Tiedeken ◽  
Cara R. Rabin ◽  
Steven J. Siegel
Keyword(s):  
Cell Death ◽  
Inbred Mouse ◽  
Mouse Strains ◽  
Inbred Mouse Strains ◽  
Adult Mice

scholarly journals Effects of chitooligosaccharide-zinc on mice ovarian function in premature ovarian failure via regulating the sestrin2/nrf2 signaling pathway

2020 ◽  
Author(s):  
Jia Li ◽  
Yuhang Chen ◽  
Jiayu Xu ◽  
Jiangying Liu ◽  
Jiacheng Fu ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Ovarian Function ◽  
Treated Group ◽  
Control Group ◽  
Chitosan Oligosaccharide ◽  
Protective Effects ◽  
Developmental Defects ◽  
Adult Mice ◽  
Nrf2 Signaling Pathway ◽  
Significant Difference

Abstract BackgroundThe chitosan oligosaccharide-zinc (COS·Zn) is also a powerful antioxidant and anti-aging scavenger, whose anti-oxidative ability immensely exceeds that of vitamin C. Therefore, this study aimed to investigate the protective effects and potential mechanism by which COS·Zn improves ovarian function and delays aging in POF.MethodsThe female KM adult mice and POF mice were divided into treated and prevention groups; these were prophylactically or therapeutically administered COS·Zn (150mg/kg·d, 300mg/kg·d) for 21 days, respectively. ResultsThe number of antral follicles in COS·Zn-treated groups was lower in the treated and prevention groups than that in the control group, respectively. Obviously, the ovarian index, the level of FSH and LH all increased notably during the 300 mg/kg·d treated group and the prevention group compared with cy/bus groups. The expression of MVH, OCT4 and PCNA in the 300 mg/kg·d treated groups and MVH in the prevention groups were remarkably increased compared with the CY/BUS group. Meanwhile, the protein levels of P53 and P16 were markedly down-regulated in the treated groups and prevention groups compared with CY/BUS, except for the expression of P53 in the prevention groups. Furthermore, the Sestrin2 (SESN2) and SOD2 proteins were significantly higher in the 150 mg/kg·d treated group compared with the CY/BUS group, but the 300 mg/kg·d and CY/BUS group showed no significant difference in the level of SESN2. Similarly, the NRF2 and SESN2 proteins were up-regulated in the prevention groups, and the change in SOD2 was not significant. The results revealed increased GSH levels in the 150 mg/kg·d and 300 mg/kg·d treated groups compared with CY/BUS group, and the same trend was also shown in the prevention groups.1Conclusion COS·Zn improves ovarian and follicular developmental defects caused by regulating the sestrin2-nrf2 signaling pathway, and these results suggest a novel product that is effective a POF prevention and treatment drug.

Differential Maintenance and Frequency-Dependent Tuning of LTP at Hippocampal Synapses of Specific Strains of Inbred Mice

2000 ◽  
Vol 84 (5) ◽  
pp. 2484-2493 ◽  
Author(s):  
Peter V. Nguyen ◽  
Steven N. Duffy ◽  
Jennie Z. Young
Keyword(s):  
Genetic Background ◽  
Pyramidal Neurons ◽  
Inbred Mice ◽  
Inbred Mouse ◽  
Mouse Strains ◽  
Synaptic Facilitation ◽  
Ca1 Pyramidal Neurons ◽  
Hippocampal Synapses ◽  
Strain Dependent

Transgenic and knockout mice are used extensively to elucidate the molecular mechanisms of hippocampal synaptic plasticity. However, genetic and phenotypic variations between inbred mouse strains that are used to construct genetic models may confound the interpretation of cellular neurophysiological data derived from these models. Using in vitro slice stimulation and recording methods, we compared the membrane biophysical, cellular electrophysiological, and synaptoplastic properties of hippocampal CA1 neurons in four specific strains of inbred mice: C57BL/6J, CBA/J, DBA/2J, and 129/SvEms/J. Hippocampal long-term potentiation (LTP) induced by theta-pattern stimulation, and by repeated multi-burst 100-Hz stimulation at various interburst intervals, was better maintained in area CA1 of slices from BL/6J mice than in slices from CBA and DBA mice. At an interburst interval of 20 s, maintenance of LTP was impaired in CBA and DBA slices, as compared with BL/6J slices. When the interburst interval was reduced to 3 s, induction of LTP was significantly enhanced in129/SvEms slices, but not in DBA and CBA slices. Long-term depression (LTD) was not significantly different between slices from these four strains. For the four strains examined, CA1 pyramidal neurons showed no significant differences in spike-frequency accommodation, membrane input resistance, and number of spikes elicited by current injection. Synaptically-evoked glutamatergic postsynaptic currents did not significantly differ among CA1 pyramidal neurons in these four strains. Since the observed LTP deficits resembled those previously seen in transgenic mice with reduced hippocampal cAMP-dependent protein kinase (PKA) activity, we searched for possible strain-dependent differences in cAMP-dependent synaptic facilitation induced by forskolin (an activator of adenylate cyclase) and IBMX (a phosphodiesterase inhibitor). We found that forskolin/IBMX-induced synaptic facilitation was deficient in area CA1 of DBA/2J and CBA/J slices, but not in BL/6J and 129/SvEms/J slices. These defects in cAMP-induced synaptic facilitation may underlie the deficits in memory, observed in CBA/J and DBA/2J mice, that have been previously reported. We conclude that hippocampal LTP is influenced by genetic background and by the temporal characteristics of the stimulation protocol. The plasticity of hippocampal synapses in some inbred mouse strains may be “tuned” to particular temporal patterns of synaptic activity. From a broader perspective, our data support the notion that strain-dependent variation in genetic background is an important factor that can influence the synaptoplastic phenotypes observed in studies that use genetically modified mice to explore the molecular bases of synaptic plasticity.

216 EFFECT OF SPERM PRETREATMENT WITH LYSOLECITHIN AND Triton X-100 ON PRONUCLEAR FORMATION AND QUALITY OF BOVINE EMBRYOS PRODUCED BY INTRACYTOPLASMIC SPERM INJECTION

2016 ◽  
Vol 28 (2) ◽  
pp. 239
Author(s):  
F. Zambrano ◽  
M. E. Arias ◽  
T. Vargas ◽  
L. Aguila ◽  
R. Felmer
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Cell Mass ◽  
Harmful Effect ◽  
Hydrolytic Enzymes ◽  
Control Group ◽  
Cleavage Rate ◽  
Blastocyst Rate ◽  
Triton X ◽  
Pronuclear Formation

In cattle, intracytoplasmic sperm injection (ICSI) has low efficiency and has application for sexed semen, semen of poor quality of high genetic value, or semen of endangered breeds. The acrosome content has a harmful effect within the oocyte because of the presence of hydrolytic enzymes. With the aim of removing the acrosome and destabilising the membranes, cryopreserved bovine spermatozoa were treated with lysolecithin (LL) and Triton X-100 (TX). Frozen straws of bull semen were thawed and the spermatozoa were selected by Percoll gradient. The selected spermatozoa were incubated at concentrations of 0.05% LL (ICSI-LL), 0.05% TX (ICSI-TX), and a control group (without treatment) for 1 min with vortex, and were then immediately washed by centrifugation at 400 × g in Sp-TALP medium. Following ICSI, oocytes were activated with 5 mM ionomycin and 5 µg mL–1 cycloheximide. Embryos were cultured in KSOM medium at 38.5°C, 5% CO2, 5% O2, and 90% N2, to Day 8 to record blastocyst rate. Pronuclear formation was evaluated at 19 h after activation, and the presumptive zygotes were fixed in methanol-glacial acetic acid (3 : 1), stained with Hoechst, and analysed with a fluorescence microscope. The results were evaluated by a chi-squared test with Bonferroni’s correction. Significance was set at P < 0.05. The quality of embryos was evaluated by counting the total cell numbers (T), inner cell mass cells (ICM), and trophectoderm cells (TE) after staining with Hoechst and propidium iodide. We injected 345 oocytes in the control group, 335 in ICSI-LL, 304 in ICSI-TX, and 351 were parthenogenetically activated (12 replicates for each group). A higher cleavage rate was observed in ICSI-TX (66%; 231/351) and ICSI-LL (62%; 207/335) compared the control group (53%; 183/345). In addition, a significantly higher blastocyst rate was observed in both treatments: 27% (82/304) and 27.5% (92 of 335) for ICSI-TX and ICSI-LL groups, respectively, compared with the control group (22.6%; 78/345). In the parthenogenetic activation group without ICSI, the cleavage rate was 41.9% (147/351) and blastocyst rate was 15.4% (54/351). Differences among treatments were analysed using one-way ANOVA after arcsine transformation, and significance was set at P < 0.05. No differences were observed in pronuclear formation (with male and female pronuclei and 2 polar bodies) for ICSI-LL (77%; 48/53), ICSI-TX (76.4%; 39/51), and the control group (71.6%; 38/53) with 3 replicates for each group. No significant differences were observed in the quality of the embryos, ICM cell number (control: 35.7 ± 2.8, ICSI-LL: 38 ± 4.5, and ICSI-TX: 40.8 ± 8.2), TE (control: 139 ± 31.5, ICSI-LL: 124 ± 10.4, and ICSI-TX: 129 ± 18.5), T (control: 170 ± 25.6, ICSI-LL: 156 ± 14.3, and ICSI-TX: 176 30.3), with 8 replicates for each group. In conclusion, this study proposed treatments able to improve the rate of development without affecting the quality of embryos produced by ICSI.

Integrative analysis reveals mouse strain-dependent responses to acute ozone exposure associated with airway macrophage transcriptional activity

2021 ◽  
Author(s):  
Adelaide Tovar ◽  
Wesley L. Crouse ◽  
Gregory J. Smith ◽  
Joseph M. Thomas ◽  
Benjamin P. Keith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Cell ◽  
Inbred Mouse ◽  
Chromatin Accessibility ◽  
Ozone Exposure ◽  
Mouse Strains ◽  
Human Populations ◽  
Transcriptional Responses ◽  
Adult Mice ◽  
Strain Dependent

Acute ozone (O3) exposure is associated with multiple adverse cardiorespiratory outcomes, the severity of which varies across individuals in human populations and inbred mouse strains. However, molecular determinants of response, including susceptibility biomarkers that distinguish who will develop severe injury and inflammation, are not well characterized. We and others have demonstrated that airway macrophages (AMs) are an important resident immune cell type that are functionally and transcriptionally responsive to O3 inhalation. Here, we sought to explore influences of strain, exposure, and strain-by-O3 exposure interactions on AM gene expression and identify transcriptional correlates of O3-induced inflammation and injury across 6 mouse strains, including 5 Collaborative Cross (CC) strains. We exposed adult mice of both sexes to filtered air (FA) or 2 ppm O3 for 3 hours, and measured inflammatory and injury parameters 21 hours later. Mice exposed to O3 developed airway neutrophilia and lung injury with strain-dependent severity. In AMs, we identified a common core O3 response signature across all strains, as well as a set of genes exhibiting strain-by-O3 exposure interactions. In particular, a prominent gene expression contrast emerged between a low- (CC017/Unc) and high-responding (CC003/Unc) strain, as reflected by cellular inflammation and injury. Further inspection indicated that differences in their baseline gene expression and chromatin accessibility profiles likely contributes to their divergent post-O3 exposure transcriptional responses. Together, these results suggest that aspects of O3-induced respiratory responses are mediated through altered AM transcriptional signatures, and further confirms the importance of gene-environment interactions in mediating differential responsiveness to environmental agents.

scholarly journals Strain variation in spermatozoal glycosidases in inbred mice

1978 ◽  
Vol 32 (2) ◽  
pp. 183-193 ◽  
Author(s):  
Steven J. Self ◽  
Bryan G. Winchester ◽  
James R. Archer
Keyword(s):  
Significant Variation ◽  
Inbred Mice ◽  
Inbred Mouse ◽  
Mouse Strains ◽  
Sperm Cells ◽  
Inbred Mouse Strains ◽  
Strain Variation ◽  
Genetic Studies ◽  
Optimal Activity ◽  
Genetical Variation

SUMMARYTen glycosidases were measured in suspensions of spermatozoa from the vasa deferentia of two inbred mouse strains and their intercrosses. Eight of these glycosidases were associated with the sperm cells and all of these showed genetical variation between the strains except α-l-fucosidase with optimal activity at pH 5·4. In contrast liver enzyme activities showed no significant variation except α-l-fucosidase. Genetic studies indicated that the variation of spermatozoal β-d-hexosaminidase, α-d-mannosidase, α-l-fucosidase and β-d-galactosidase are inherited at autosomal loci and α-d-galactosidase variation shows X-linked inheritance. We propose a new provisional gene symbol (Afuc-2) for a spermatozoal variant of α-l-fucosidase.

scholarly journals Substrains of Inbred Mice Differ in Their Physical Activity as a Behavior

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Dario Coletti ◽  
Emanuele Berardi ◽  
Paola Aulino ◽  
Eleonora Rossi ◽  
Viviana Moresi ◽  
...  
Keyword(s):  
Physical Activity ◽  
Wheel Running ◽  
Average Distance ◽  
Inbred Mice ◽  
Inbred Mouse ◽  
Inbred Mouse Strain ◽  
Time Frame ◽  
Mouse Strains ◽  
Muscle Adaptation ◽  
Voluntary Activity

Recent studies strengthen the belief that physical activity as a behavior has a genetic basis. Screening wheel-running behavior in inbred mouse strains highlighted differences among strains, showing that even very limited genetic differences deeply affect mouse behavior. We extended this observation to substrains of the same inbred mouse strain, that is, BALB/c mice. We found that only a minority of the population of one of these substrains, the BALB/c J, performs spontaneous physical activity. In addition, the runners of this substrain cover a significantly smaller distance than the average runners of two other substrains, namely, the BALB/c ByJ and the BALB/c AnNCrl. The latter shows a striking level of voluntary activity, with the average distance run/day reaching up to about 12 kilometers. These runners are not outstanders, but they represent the majority of the population, with important scientific and economic fallouts to be taken into account during experimental planning. Spontaneous activity persists in pathological conditions, such as cancer-associated cachexia. This important amount of physical activity results in a minor muscle adaptation to endurance exercise over a three-week period; indeed, only a nonsignificant increase in NADH transferase+ fibers occurs in this time frame.

Effect of sperm pretreatment with sodium hydroxide and dithiothreitol on the efficiency of bovine intracytoplasmic sperm injection

2014 ◽  
Vol 26 (6) ◽  
pp. 847 ◽  
Author(s):  
M. E. Arias ◽  
R. Sánchez ◽  
J. Risopatrón ◽  
L. Pérez ◽  
R. Felmer
Keyword(s):  
Embryonic Development ◽  
Intracytoplasmic Sperm Injection ◽  
Membrane Integrity ◽  
Control Group ◽  
Developmental Potential ◽  
Bovine Spermatozoa ◽  
Pronuclear Formation ◽  
First Time ◽  
In Vitro Embryonic Development

The efficiency of intracytoplasmic sperm injection (ICSI) in bovines is lower than in other species due, in part, to a lack of optimal conditions for its implementation; this has hindered the achievement of high rates of embryonic development and the birth of live offspring. The aim of the present study was to evaluate the effects of pretreatment of bovine spermatozoa with NaOH and dithiothreitol (DTT) on the viability, plasma membrane integrity, DNA fragmentation and in vitro developmental potential of embryos generated by ICSI. Following pretreatment of spermatozoa with 5 mM DTT for 20 min and a low concentration of NaOH (1 mM for 60 min), there were fewer live and acrosome reacted spermatozoa (44% and 34%, respectively) than in the control group without treatment (82%). Spermatozoa subjected to higher alkali concentrations (10–50 mM) were mostly dead and reacted. However, pronuclear formation, cleavage, blastocyst rate and embryo quality did not differ between these pretreatment groups and the untreated control group. In conclusion, we have described, for the first time, the effects of NaOH treatment on bovine spermatozoa and subsequent in vitro embryonic development after ICSI, and have demonstrated that pretreatment of bovine spermatozoa with NaOH or DTT is not necessary for an appropriate in vitro embryo development in this species.

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